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assessment of phylloplane yeasts on selected mediterranean plants by fish with group- and species-specific oligonucleotide probes.a previous culture-dependent survey of phylloplane yeasts from selected mediterranean plants showed that a few species were present in high densities in almost all leaf samples, regardless of the plant type, location or sampling season. however, a few species appeared to be restricted to cistus albidus leaves, namely cryptococcus cistialbidi. here, we describe a culture-independent fish assay to detect and quantify whole yeast cells in leaf washings. after optimization, the technique was used to ...201019817863
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