| the complete mitochondrial genome of the javeline goby acanthogobius hasta (perciformes, gobiidae) and phylogenetic considerations. | we isolated acanthogobius hasta mitochondrial dna by long-polymerase chain reaction (long-pcr) with conserved primers, and sequenced this mitogenome with primer walking. the resultant a. hasta mitochondrial dna sequence was found to consist of 16,663 bp with a structural organization conserved relative to that of other fish. in this paper, we report the basic characteristics of the a. hasta mitochondrial genome including structural organization, base composition of rrnas and the trnas and protei ... | 2004 | 15246526 |
| effect of waterborne copper exposure on growth, hepatic enzymatic activities and histology in synechogobius hasta. | the present study was conducted to determine growth, hepatic enzymatic activities and histology in synechogobius hasta exposed to waterborne copper concentrations of 0 (control), 0.15 and 0.3 mg cu/l, respectively, for 15 days, and explore whether waterborne copper exposure could induce the fatty liver syndrome for the fish species. growth (wg and sgr) declined, but hsi increased in s. hasta with increasing waterborne copper levels (p<0.05). waterborne copper exposure also significantly increase ... | 2010 | 20637506 |
| effects of waterborne acephate exposure on antioxidant responses and acetylcholinesterase activities in synechogobius hasta. | the present study was conducted to determine the 24, 48, 72, and 96-h median lethal concentration (lc50) of acephate and investigate the antioxidant response and acetylcholinesterase (ache) activities in liver, gill, and spleen of synechogobius hasta exposed to 0 (control), 5, and 10 mg/l acephate, at the fixed interval time of 24, 48, 72, and 96 h, respectively. lc50 value was 60.83 mg/l at 24 h, 51.36 mg/l at 48 h, 47.07 mg/l at 72 h and 40.13 mg/l at 96 h, respectively. dismutase (sod), catal ... | 2011 | 21462291 |
| effect of waterborne zinc exposure on metal accumulation, enzymatic activities and histology of synechogobius hasta. | the present study was conducted to determine the metal accumulation, antioxidant enzymatic response, hepatic intermediary metabolism and histological changes in synechogobius hasta exposed to 0.35 (control), 9.7 and 19.2mg/l zn, respectively, on the 0, 4th, 8th and 12th day. waterborne zn exposure significantly reduced hepatosomatic index, hepatic lipid contents and fatty liver occurrence rate, increased zn, fe and mn contents and reduced the contents of cu and ca in liver, and increased muscle ... | 2011 | 21764133 |
| effects of calcium and copper exposure on lipogenic metabolism, metal element compositions and histology in synechogobius hasta. | the present study was conducted to evaluate the effects of calcium (ca) and copper (cu) exposure on growth performance, metal element composition, enzymatic activities and histology in synechogobius hasta and tested the hypothesis that ca could protect against cu-induced toxicity in the fish species. three hundred sixty s. hasta (initial mean weight 22.6 ± 0.2 g, mean ± sem) were stocked in 18 circular fiberglass tanks (water volume: 300 l), 9 of which were pre-exposed to ca at a rate of 600 mg ... | 2013 | 23743594 |
| complete sequence of the mitochondrial genome of odontamblyopus rubicundus (perciformes: gobiidae): genome characterization and phylogenetic analysis. | odontamblyopus rubicundus is a species of gobiid fishes, inhabits muddy-bottomed coastal waters. in this paper, the first complete mitochondrial genome sequence of o. rubicundus is reported. the complete mitochondrial genome sequence is 17119 bp in length and contains 13 protein-coding genes, two rrna genes, 22 trna genes, a control region and an l-strand origin as in other teleosts. most mitochondrial genes are encoded on h-strand except for nd6 and seven trna genes. some overlaps occur in prot ... | 2013 | 24371164 |
| molecular cloning and mrna tissue expression of thyroid hormone receptors in yellow catfish pelteobagrus fulvidraco and javelin goby synechogobius hasta. | thyroid hormones (ths) play a pivotal role in many physiological functions in vertebrates, including fish. their effects are mediated by thyroid hormone receptors (trs), which are members of the nuclear hormone receptor superfamily. in this study, full-length cdna sequences of trs from yellow catfish pelteobagrus fulvidraco and javelin goby synechogobius hasta were cloned and their mrna tissue expression profiles were determined. in p. fulvidraco, the validated cdnas encoding for trα and trβ wer ... | 2014 | 24380707 |
| effects of copper and cadmium on lipogenic metabolism and metal element composition in the javelin goby (synechogobius hasta) after single and combined exposure. | the present study was performed to determine the effects of single and combined exposure of copper (cu) and cadmium (cd) on lipogenic metabolism and metal element composition of javelin goby synechogobius hasta. two hundred and forty uniform-sized s. hasta (initial mean weight 20.3 ± 0.3 g [mean ± sem throughout]; initial body length 15.2 ± 0.2 cm) were randomly assigned to 12 fiberglass tanks (water volume 300 l) with 20 fish/tank. the fish were exposed to four treatments with different cu and ... | 2014 | 24595737 |
| [characteristics and phylogenetic analysis of mitochondrial genome in the gobies]. | the vast number of species, small size and high variation of morphology make the morphological identification and classification of gobies very difficult. in this study, the complete mitochondrial genome (mitogenome) of 26 species of gobies was analyzed, aiming at accumulating the molecular information on the identification, classification and molecular evolution of gobies. the results showed that the gene composition and arrangement of mitogenome of gobies are similar to most vertebrates. due t ... | 2013 | 24645349 |
| time-dependent effects of waterborne copper exposure influencing hepatic lipid deposition and metabolism in javelin goby synechogobius hasta and their mechanism. | the present study was conducted to determine the time-course of waterborne chronic copper (cu) exposure effects influencing hepatic lipid deposition and metabolism in javelin goby synechogobius hasta and their mechanisms. s. hasta were exposed to four waterborne cu concentrations (2 (control), 18, 38 and 55 μg cu/l) for 60 days. sampling occurred on day 30 and day 60, respectively. survival decreased and hepatic cu content increased with increasing cu levels. on day 30, cu exposure increased hep ... | 2014 | 25087000 |
| [determination of 19 antibiotic and 2 sulfonamide metabolite residues in wild fish muscle in mariculture areas of laizhou bay using accelerated solvent extraction and high performance liquid chromatography-tandem mass spectrometry]. | a sample preparation and analytical method with accelerated solvent extraction (ase) and high performance liquid chromatography-tandem mass spectrometry (hplc-ms/ ms) was developed to detect 19 antibiotic (9 sulfonamides, 4 quinolones, 3 macrolides and 3 others) and 2 sulfonamide metabolite residues in fish muscle. the target compounds were extracted using ase and purified simultaneously by a c18 resin in the extraction cell. the extracts were evaporated to dryness, and redissolved with the init ... | 2014 | 25902638 |
| carnitine palmitoyltransferase i gene in synechogobius hasta: cloning, mrna expression and transcriptional regulation by insulin in vitro. | we cloned seven complete cpt i cdna sequences (cpt i α1a-1a, cpt i α1a-1b, cpt i α1a-1c, cpt i α1a-2, cpt i α2a, cpt i α2b1a, cpt i β) and a partial cdna sequence (cpt i α2b1b) from synechogobius hasta. phylogenetic analysis shows that there are four cpt i duplications in s. hasta, cpt i duplication resulting in cpt i α and cpt i β, cpt i α duplication producing cpt i α1 and cpt i α2, cpt i α2 duplication generating cpt i α2a and cpt i α2b, and cpt i α2b duplication creating cpt i α2b1a and cpt ... | 2016 | 26506441 |
| effect and mechanism of waterborne prolonged zn exposure influencing hepatic lipid metabolism in javelin goby synechogobius hasta. | the present study was conducted to determine the effect and mechanism of waterborne zn exposure influencing hepatic lipid deposition and metabolism in javelin goby synechogobius hasta. s. hasta were exposed to four waterborne zn concentrations (zn 0.005 [control], 0.18, 0.36 and 0.55 mg l(-1) , respectively) for 60 days. sampling occurred at days 20, 40 and 60, respectively. zn exposure increased zn content, declined hepatic lipid content and reduced viscerosomatic and hepatosomatic indices and ... | 2016 | 26602879 |
| de novo characterization of the liver transcriptome of javelin goby synechogobius hasta and analysis of its transcriptomic profile following waterborne copper exposure. | previous studies have investigated the physiological responses to chronic copper (cu) exposure in the liver of synechogobius hasta; however, little information is available on the underlying molecular mechanisms. in an effort to better understand the mechanisms of cu toxicity and to illuminate global gene expression patterns modulated by cu exposure, we obtained the liver transcriptome information of s. hasta by rna sequencing (rna-seq) technology and also investigated the differential expressio ... | 2016 | 26719065 |
| bioaccumulation characteristics of polybrominated diphenyl ethers in the marine food web of bohai bay. | in recent years, polybrominated diphenyl ethers (pbdes) are ubiquitous environmental contaminants, but its bioaccumulation and debromination in biota have remained largely unclear. in this study, we analyzed six pbdes (bde47, 99, 100, 153, 154, and 209) in various tissues (i.e., viscera, muscle, and gill) of 11 types of marine organisms including zooplankton, invertebrate and fish. the concentrations of six pbde including bde209 in marine organisms ranged from 0.75 to 7.29 ng/g dry weight, bde20 ... | 2016 | 26857988 |
| fe reduced hepatic lipid deposition in synechogobius hasta exposed to waterborne cu. | recent evidences suggested that fe influenced cu metabolism in vertebrates. the present study was conducted to test the hypothesis that fe could alleviate cu-induced change of lipid deposition in the fish species. synechogobius hasta were exposed to 0, 0.606 and 1.212μm cu, in combination with 0 and 1.128μm fe, respectively. sampling occurred on day 28 and day 56, respectively. growth performance, hepatic lipid deposition, fe and cu level, and activities and mrna expression of enzymes and genes ... | 2016 | 26945540 |
| endoplasmic reticulum stress and dysregulation of calcium homeostasis mediate cu-induced alteration in hepatic lipid metabolism of javelin goby synechogobius hasta. | the present study was conducted to investigate the effect of cu exposure on endoplasmic reticulum (er) stress and ca(2+) homeostasis, and also explore the underlying mechanism of the er stress and ca(2+) homeostasis in the cu-induced change of hepatic lipid metabolism in javelin goby synechogobius hasta. to this end, four experiments were conducted. in experiment 1, the full-length cdna sequences of two er molecular chaperones [glucose-regulated protein 78 (grp78) and calreticulin (crt)] and thr ... | 2016 | 26991751 |
| isolation and expression analysis of stat members from synechogobius hasta and their roles in leptin affecting lipid metabolism. | signal transducers and activators of transcription proteins (stats) act as important mediators in multiple biological processes induced by a large number of cytokines. in the present study, full-length cdna sequences of seven stat members, including some splicing variants different from those in mammals, were obtained from synechogobius hasta. the phylogenetic analysis revealed that the seven stat members were derived from paralogous genes that might have arisen by whole genome duplication (wgd) ... | 2016 | 27011172 |
| complete mitochondrial genome of javeline goby (synechogobius hasta). | in this study, we cloned and sequenced the complete mitochondrial dna (mtdna) of synechogobius hasta to characterize and compare their mitochondrial genomes. the total length of the mitochondrial genome is 16,655 bp with an accession number km891736. the organization of the mitochondrial genomes was similar to those reported from other fish mitochondrial genomes containing 37 genes (13 protein-coding genes, 2 ribosomal rna and 22 transfer rnas) and a major non-coding control region. except for n ... | 2016 | 27607305 |
| jak family members: molecular cloning, expression profiles and their roles in leptin influencing lipid metabolism in synechogobius hasta. | janus kinase (jak) is a family of non-receptor tyrosine kinases that participate in transducing cytokine signals from the external environment to the nucleus in various biological processes. currently, information about their genes structure and evolutionary history has been extensively studied in mammals as well as in several fish species. by contrast, limited reports have addressed potential role of diverse jak in signaling responses to leptin in fish. in this study, we identified and characte ... | 2017 | 27789245 |
| morphological and genetic characterization of kudoa whippsi (myxosporea: multivalvulida) from cheilodactylus zonatus in the western pacific ocean off japan, and two new kudoa spp. (k. akihitoi n. sp. and k. empressmichikoae n. sp.) from acanthogobius hasta in the sea of ariake, japan. | molecular genetic characterization using the ribosomal rna (rdna) gene accrues a wealth of knowledge regarding the true nature of species diversity of kudoa meglitsch, 1947 (myxozoa: myxosporea: multivalvulida) and the biogeographical relationships of isolates from different host fish and sea areas. in the present study, we characterized morphologically and genetically three kudoa spp. with four shell valves and polar capsules (sv/pc), forming pseudocysts in the myofiber of trunk muscles of chei ... | 2017 | 27928682 |
| role and mechanism of the ampk pathway in waterborne zn exposure influencing the hepatic energy metabolism of synechogobius hasta. | previous studies have investigated the physiological responses in the liver of synechogobius hasta exposed to waterborne zinc (zn). however, at present, very little is known about the underlying molecular mechanisms of these responses. in this study, rna sequencing (rna-seq) was performed to analyse the differences in the hepatic transcriptomes between control and zn-exposed s. hasta. a total of 36,339 unigenes and 1,615 bp of unigene n50 were detected. these genes were further annotated to the ... | 2016 | 27934965 |
| complete mitochondrial genome of yellowfin goby (acanthogobius hasta). | yellowfin goby (acanthogobius hasta) belongs to the perciformes gobiidae. in this study, we sequenced the complete mitochondrial genome of a. hasta. the complete mitochondrial genome was determined to be 16,663 bp in length including 13 protein-coding genes, 22 transfer rnas (trnas), 2 ribosomal rnas (rrnas) and 1 control region. the complete mitochondrial genome of a. gueldenstaedti provides basic genome data for relative studies on acipenseriformes. | 2016 | 25082453 |
| three new species of pseudodactylogyrus (monogenea: pseudodactylogyridae) from australian eels. | three species of pseudodactylogyrus gusev, 1965 (monogenea: pseudodactylogyridae) were collected from the gills of anguilla reinhardtii steindachner and a. australis richardson from several localities in australia and eels imported to japan from australia. pseudodactylogyrus gusevi sp. n. from a. reinhardtii (type host) and a. australis in queensland, australia is most similar to p. bini (kikuchi, 1929), but can be differentiated by the shorter male copulatory tube, heavy sclerotisation of the v ... | 2015 | 26374824 |
| antioxidant responses, hepatic intermediary metabolism, histology and ultrastructure in synechogobius hasta exposed to waterborne cadmium. | the present study was conducted to determine antioxidant responses, hepatic intermediary metabolism, histology and ultrastructure in synechogobius hasta after a 15-days of waterborne cadmium (cd) exposure at the concentrations of 0 (control), 0.10, 0.17 and 0.29 mg cd/l (corresponding to 0, 12.6%, 21.5% and 36.7% of 96 h lcôéàôéç), respectively. growth performance and survival declined, but hepatosomatic index (hsi) increased with increasing waterborne cd levels (p < 0.05). waterborne cd exposur ... | 2011 | 21392825 |
| gross and fine anatomy of the respiratory vasculature of the mudskipper, periophthalmodon schlosseri (gobiidae: oxudercinae). | to illustrate vascular modification accompanying transition from aquatic to amphibious life in gobies, we investigated the respiratory vasculatures of the gills and the bucco-opercular cavities in one of the most terrestrially-adapted mudskippers, periophthalmodon schlosseri, using the corrosion casting technique. the vascular system of pn. schlosseri retains the typical fish configuration with a serial connection of the gills and the systemic circuits, suggesting a lack of separation of o(2)-po ... | 2011 | 21344480 |
| characteristics of trophic transfer of polychlorinated biphenyls in marine organisms in incheon north harbor, korea. | the trophic transfer of polychlorinated biphenyls (pcbs) was characterized for zooplankton (primarily paracalanus spp. and acartia spp.), pacific oyster (crassostrea gigas), shore crab (hemigrapsus penicillatus), and goby (acanthogobius hasta) in the aquatic system of incheon north harbor, korea. the congener pattern in the species was clearly divided by the main pcb uptake route. compared with zooplankton and oyster, the fraction of heavier homologues increased in crab and goby that take pcbs f ... | 2002 | 11951959 |
| protective effects of calcium pre-exposure against waterborne cadmium toxicity in synechogobius hasta. | the present study was performed to evaluate the effects of calcium (ca) pre-exposure and then waterborne cadmium (cd) exposure on metal element accumulation, enzymatic activities, histology, and ultrastructure in synechogobius hasta and test the hypothesis that ca could protect against cd-induced toxicity in the fish species. three hundred sixty fish [initial mean weight 25.5 ± 0.1 g (mean ± sem)] were stocked in 18 circular fiberglass tanks (water volume: 300 l), 9 of which were pre-exposed to ... | 2013 | 23467709 |
| trans-10,cis-12 conjugated linoleic acid improved growth performance, reduced lipid deposition and influenced cpt i kinetic constants of juvenile synechogobius hasta. | trans-10,cis-12 (t10c12) conjugated linoleic acid (cla) reduced body lipid deposition in various experimental animals, but the mechanisms involved were still emerging. carnitine palmitoyltransferase i (cpt i) catalyzes an important regulatory step in lipid metabolism. at present, no studies, to our knowledge, have evaluated the kinetic constants influenced by dietary cla in fish. in the present study, we tested the hypothesis that changes in body lipid content in fish as a response to dietary t1 ... | 2013 | 23322076 |
| effects of waterborne chronic copper exposure on hepatic lipid metabolism and metal-element composition in synechogobius hasta. | the present study was conducted to determine hepatic lipid metabolism and metal-element composition in synechogobius hasta exposed to waterborne chronic copper (cu) concentrations of control, 57, and 118 μg cu/l, respectively, for 30 days. growth decreased, but hepatosomatic index, viscerosomatic index, and hepatic lipid content increased with increasing waterborne cu levels. staining with oil red o showed extensive steatosis in liver of cu-exposed fish. cu exposure increased hepatic 6-phosphogl ... | 2013 | 23229194 |
| complete mitochondrial genome of the asian freshwater goby synechogobius ommaturus (perciformes, gobioidei). | the asian freshwater goby, synechogobius ommaturus (perciformes, gobiidae) is a big goby inhabiting shallow sandy-muddy bottoms in temperate riverine estuaries and inner bays, mainly distributed in china, japan, and the korean peninsula. in this paper, the complete mitochondrial genome of s. ommaturus was firstly determined. the genome is 16,660 bp in length and consists of 13 protein-coding genes, 22 trna genes, 2 ribosomal rna genes, and 2 main non-coding regions (the control region and the or ... | 2013 | 22963663 |
| seventeen polymorphic microsatellite markers developed for the javelin goby, synechogobius hasta (gobiidae). | synechogobius hasta is an important commercial marine fish with distinctive features of rapid growth and short lifespan. we isolated and characterized 17 microsatellite markers for s. hasta using a (gt)(13)-enriched genomic library. polymorphism was assessed in 48 individuals from a single population collected from the northern coastal waters of the yellow sea. the number of alleles per locus ranged from 2 to 23, with a mean of 11.3. the observed and expected heterozygosities ranged from 0.130 t ... | 2012 | 22653595 |
| population genetic structure and larval dispersal potential of spottedtail goby synechogobius ommaturus in the north-west pacific. | larval dispersal may have an important effect on genetic structure of benthic fishes. to examine the population genetic structure of spottedtail goby synechogobius ommaturus, a 478 base pair (bp) fragment of the hypervariable portion of the mtdna control region was sequenced and used to interpret life-history characteristics and larval dispersal strategy. individuals (n = 186) from 10 locations on the coasts of china and korea were analysed and 44 haplotypes were obtained. the levels of haplotyp ... | 2010 | 20646163 |
| five metal elements homeostasis-related genes in synechogobius hasta: molecular characterization, tissue expression and transcriptional response to cu and fe exposure. | two isoforms of cu transporter (ctr1 and ctr2) and metallothionein (mt1 and mt2), and divalent metal ion transporter 1 (dmt1) were cloned and characterized in synechogobius hasta, respectively. the protein sequences of s. hasta ctrs possessed two methionine-rich regions (mxm and mxxxm) and three transmembrane regions. at the c-terminus, ctr1 contained a sequence of conserved cysteine and histidine residues (hch), while ctr2 did not contain the conserved sequence. the protein sequence of s. hasta ... | 2016 | 27323292 |
| effects of waterborne cu exposure on intestinal copper transport and lipid metabolism of synechogobius hasta. | the present study was conducted to explore the effects of waterborne cu exposure on intestinal cu transport and lipid metabolism of synechogobius hasta. s. hasta were exposed to 0, 0.4721 and 0.9442μm cu, respectively. sampling occurred on days 0, 21 and 42, respectively. growth performance, intestinal lipid deposition, cu content, and activities and mrna expression of enzymes and genes involved in cu transport and lipid metabolism were analyzed. cu exposure decreased wg and sgr on days 21 and 4 ... | 2016 | 27509383 |