| galactosylation at side chains of branched cyclodextrins by various beta-galactosidases. | the galactosyl transfer reaction to branched cyclodextrins (cds) was investigated using lactose as a donor substrate and branched cds as acceptors by various beta-galactosidases. bacillus circulans beta-galactosidase synthesized galactosyl transfer products to branched cds, of which the galactose residues were linked at side chains of branched cds, not directly at cd rings. aspergillus oryzae and penicillium multicolor beta-galactosidases also produced derivatives galactosylated at side chains o ... | 1992 | 1368300 |
| isochromophilones iii-vi, inhibitors of acyl-coa:cholesterol acyltransferase produced by penicillium multicolor fo-3216. | new azaphilones named isochromophilones iii-vi were isolated from the culture broth of penicillium multicolor fo-3216 as inhibitors of acyl-coa: cholesterol acyltransferase (acat). their structures were elucidated by nmr and other spectroscopic analyses. the ic50 values of isochromophilones iii, iv, v and vi for acat activity in an enzyme assay using rat liver microsomes were calculated to be 110, 50, 50 and 120 microm, respectively. | 1995 | 7649870 |
| isochromophilones i and ii, novel inhibitors against gp120-cd4 binding produced by penicillium multicolor fo-2338. i. screening, taxonomy, fermentation, isolation and biological activity. | isochromophilones i and ii, the first novel gp120-cd4 binding inhibitors of microbial origin, were isolated from a cultured broth of a soil fungus designated as penicillium multicolor fo-2338. these compounds were obtained as yellow powders from the cultured broth together with the known related compounds sclerotiorin, ochrephilone and rubrorotiorin. isochromophilones i and ii (c23h25o5cl and c22h27o4cl, respectively) have an azaphilone skeleton and a chlorine atom. isochromophilones strongly in ... | 1995 | 7649871 |
| preparation, isolation, and characterization of novel heterogeneous branched cyclomalto-oligosaccharides having beta-d-galactosyl residue(s) on the side chain. | transgalactosylated products of branched cyclodextrins (glucosyl-alpha cd, -beta cd, -gamma cd, and maltosyl-alpha cd, -beta cd, -gamma cd) were synthesized by beta-d-galactosidases from bacillus circulans and penicillium multicolor using lactose as a donor substrate and branched cds as acceptors. eighteen beta-d-galactosylated branched cds were isolated and purified by hplc. their structures were elucidated by fabms and 13c nmr spectroscopies, and methylation analysis. the chromatographic behav ... | 1993 | 8431940 |
| the synthesis of galactopyranosyl derivatives with beta-galactosidases of different origins. | beta-galactosidases from four different sources were used to catalyze the transfer of beta-d-galactopyranosyl from 4-nitrophenyl-beta-d-galactopyranoside to a hydroxyl group of 2-acetamido-2-deoxy-galactopyranose in the synthesis of gal beta (1-3)galnac (1), gal beta (1-4)galnac (2) and gal beta (1-6)galnac (3), in triethyl phosphate buffered solutions (20-60%). when beta-galactosidases from penicillium multicolor and aspergillus oryzae were used as the catalysts, the beta (1-6)-linked disacchar ... | 1996 | 8870243 |
| enzymatic synthesis, isolation, and analysis of novel alpha- and beta-galactosyl-cycloisomalto-octaoses. | novel branched cycloisomalto-octaoses (ci8s) were enzymatically synthesized by transgalactosylation with alpha-galactosidase from coffee bean and beta-galactosidase preparations from penicillium multicolor and bacillus circulans, using melibiose and lactose as donor substrates, and ci8 which is a cyclic homogeneous oligosaccharide composed of eight glucose units bound by alpha-(1-->6)-linkages, as an acceptor. alpha-galactosyl-ci8s and beta-galactosyl-ci8s obtained were isolated and purified by ... | 1997 | 9648258 |
| an alpha-l-fucosidase from penicillium multicolor as a candidate enzyme for the synthesis of alpha (1-->3)-linked fucosyl oligosaccharides by transglycosylation. | a new alpha-l-fucosidase was partially purified from the culture broth of penicillium multicolor, which was available commercially as a freeze dried powder by the name of lactase-p. this enzyme catalysed the transglycosylation of fucose residue of p-nitrophenyl-alpha-l-fucopyranoside to give alpha-l-fuc-(1-->3)-d-glc or alpha-l-fuc-(1-->3)-d-glcnac regioselectively. this enzyme was more stable in the organic co-solvents than the alpha-fucosidase from aspergillus niger, which was also proposed pr ... | 1998 | 9720243 |
| hydrolysis of beta-galactosyl ester linkage by beta-galactosidases. | p-hydroxybenzoyl beta-galactose (phb-gal) was synthesized chemically to examine the hydrolytic activity of beta-galactosyl ester linkage by beta-galactosidases. the enzyme from penicillium multicolor hydrolyzed the substrate as fast as p-nitrophenyl beta-galactoside (pnp-gal), a usual substrate with a beta-galactosidic linkage. the enzymes from escherichia coli and aspergillus oryzae hydrolyzed phb-gal with almost the same rates as pnp-gal. the enzymes from bacillus circulans, saccharomyces frag ... | 2000 | 10993159 |
| enzymatic synthesis of fucose-containing disaccharides employing the partially purified alpha-l-fucosidase from penicillium multicolor. | the alpha-l-fucp-(1 --> 3)-d-glcpnac disaccharide structure is a vital core unit of the oligosaccharide components of glycoconjugates isolated from human milk and blood group substances. alpha-l-fucosidase from penicillium multicolor catalyses the transfer of l-fucose from donor structures such as alpha-l-fucpopnp and alpha-l-fucpf to various glcpnac derivatives and glcp, forming alpha-(1 --> 3) linkages. the synthesis of several biologically relevant disaccharides including alpha-l-fucp-(1 --> ... | 2000 | 11072836 |
| toxicity evaluation of a beta-galactosidase preparation produced by penicillium multicolor. | tilactase is a beta-galactosidase enzyme preparation having lactase activity produced from the fungus penicillium multicolor. the safety of tilactase was investigated in rats, dogs, and rabbits. adult and juvenile rats administered 0, 500, 1000, or 4000 mg/kg bw/day of the enzyme preparation by gavage for 35 days, and dogs administered 0, 200, 500, or 1000 mg/kg bw/day in capsules for 30 days, exhibited no significant dose-related changes in body weights, feed consumption, organ weights, urinaly ... | 2004 | 15546682 |
| hyperinduction of nitrilases in filamentous fungi. | 2-cyanopyridine proved to act as a powerful nitrilase inducer in aspergillus niger k10, fusarium solani o1, fusarium oxysporum ccf 1414, fusarium oxysporum ccf 483 and penicillium multicolor ccf 2244. valeronitrile also enhanced the nitrilase activity in most of the strains. the highest nitrilase activities were produced by fungi cultivated in a czapek-dox medium with both 2-cyanopyridine and valeronitrile. the specific nitrilase activities of these cultures were two to three orders of magnitude ... | 2006 | 16909267 |
| enzymatic synthesis of gentiooligosaccharides by transglycosylation with beta-glycosidases from penicillium multicolor. | a crude enzyme preparation from penicillium multicolor efficiently produced mainly gentiotriose to gentiopentaose (d.p. 3-5) by transglycosylation using a high concentration of gentiobiose as the substrate. the resulting gentiotriose was examined in a gustatory sensation test using human volunteers, and was determined to have one-fifth of the bitterness of gentiobiose. the crude enzyme preparation was analyzed by chromatography to determine the enzyme responsible for formation of the gentiooligo ... | 2009 | 19362709 |
| discovery of secondary metabolites in an extractive liquid-surface immobilization system and its application to high-throughput interfacial screening of antibiotic-producing fungi. | an extractive liquid-surface immobilization (ext-lsi) system, which consists of a hydrophobic organic solvent (an upper phase), a fungal cell-ballooned microsphere layer (a middle phase) and a liquid medium (a lower phase), is a unique interfacial cultivation system for fungi. the fungal cells growing at the interface between the organic and aqueous phases efficiently produce hydrophobic metabolites, which are continuously extracted into the organic phase, and/or hydrophilic metabolites that mig ... | 2015 | 25966852 |
| beta-glycosylamidine as a ligand for affinity chromatography tailored to the glycon substrate specificity of beta-glycosidases. | an affinity adsorbent for beta-glycosidases has been prepared by using beta-glycosylamidine as a ligand. beta-glucosylamidine and beta-galactosylamidine, highly potent and selective inhibitors of beta-glucosidases and beta-galactosidases, respectively, were immobilized by a novel one-pot procedure involving the addition of a beta-glycosylamine and 2-iminothiolane.hcl simultaneously to a matrix modified with maleimido groups via an appropriate spacer to give an affinity adsorbent for beta-glucosi ... | 2003 | 12829393 |
| mode of action of a β-(1→6)-glucanase from penicillium multicolor. | β-(1→6)-glucanase from the culture filtrate of penicillium multicolor lam7153 was purified by ammonium sulfate precipitation, followed by cation-exchange and affinity chromatography using gentiotetraose (gen₄) as ligand. the hydrolytic mode of action of the purified protein on β-(1→6)-glucan (pustulan) was elucidated in real time during the reaction by hpaec-pad analysis. gentiooligosaccharides (dp 2-9, gen₂₋₉), methyl β-gentiooligosides (dp 2-6, gen₂₋₆ β-ome), and p-nitrophenyl β-gentiooligosid ... | 2013 | 23246473 |
| studies in the biochemistry of micro-organisms. metabolic products of penicillium multicolor g.-m. and p. with special reference to sclerotiorin. | | 1952 | 13018222 |
| studies in the biochemistry of micro-organisms. 113. pencolide, a nitrogen-containing metabolite of penicillium multicolor grigorieva-manilova and poradielova. | | 1963 | 13971137 |
| isochromophilones i and ii, novel inhibitors against gp120-cd4 binding produced by penicillium multicolor fo-2338. ii. structure elucidation. | the structures of isochromophilones i and ii, new gp120-cd4 binding inhibitors isolated from a cultured broth of penicillium multicolor fo-2338, were elucidated by nmr experiments. both of compounds have an azaphilone skeleton substituted by a chlorine atom at c-5 and a side chain, 3,5-dimethyl-1,3-heptadien at c-3. additionally, isochromophilone i has a gamma-lactone ring, and isochromophilone ii has 2-oxopropyl moiety instead of a gamma-lactone ring. | 1995 | 7649872 |
| new brominated and halogen-less derivatives and structure-activity relationship of azaphilones inhibiting gp120-cd4 binding. | novel brominated and halogen-less azaphilone (oxoisochromane) derivatives, 5-bromoochrephilone and dechloroisochromophilone iv, and known derivatives, dechloroisochromophilone iii and isorotiorin, were isolated from the culture broth of a producing organism of isochromophilones i and ii (azaphilones inhibiting gp120-cd4 binding), penicillium multicolor fo-2338, fermented in a medium containing potassium bromide. nineteen azaphilone-related compounds isolated from the above strain and from other ... | 1998 | 9918393 |
| 8-o-methylsclerotiorinamine, antagonist of the grb2-sh2 domain, isolated from penicillium multicolor. | a new secondary metabolite, 8-o-methylsclerotiorinamine (1), was isolated from a strain of penicillium multicolor, and its structure was established using nmr spectroscopy and chemical evidence. the metabolite inhibited significantly the binding between the grb2-sh2 domain and the phosphopeptide derived from the shc protein and also blocked the protein-protein interactions of grb2-shc in cell-based experiments, with ic(50) values of 5.3 and 50 microm, respectively. | 2000 | 11000046 |
| isolation and characterization of a beta-primeverosidase-like enzyme from penicillium multicolor. | p-nitrophenyl and eugenyl beta-primeveroside (6-o-beta-d-xylopyranosyl-beta-d-glucopyranoside) hydrolytic activity was found in culture filtrate from penicillium multicolor iam7153, and the enzyme was isolated. the enzyme was purified as a beta-primeverosidase-like enzyme by precipitation with ammonium sulfate followed by successive chromatographies on phenyl sepharose, mono q, and beta-galactosylamidine affinity columns. the molecular mass was estimated to be 50 kda by sds-page and gel filtrati ... | 2006 | 16556987 |