| mycobacterium pseudoshottsii sp. nov., a slowly growing chromogenic species isolated from chesapeake bay striped bass (morone saxatilis). | a group of slowly growing photochromogenic mycobacteria was isolated from chesapeake bay striped bass (morone saxatilis) during an epizootic of mycobacteriosis. growth characteristics, acid-fastness and 16s rrna gene sequencing results were consistent with those of the genus mycobacterium. biochemical reactions, growth characteristics and mycolic acid profiles (hplc) resembled those of mycobacterium shottsii, a non-pigmented mycobacterium also isolated during the same epizootic. sequencing of th ... | 2005 | 15879246 |
| globally distributed mycobacterial fish pathogens produce a novel plasmid-encoded toxic macrolide, mycolactone f. | mycobacterium ulcerans and mycobacterium marinum are closely related pathogens which share an aquatic environment. the pathogenesis of these organisms in humans is limited by their inability to grow above 35 degrees c. m. marinum causes systemic disease in fish but produces localized skin infections in humans. m. ulcerans causes buruli ulcer, a severe human skin lesion. at the molecular level, m. ulcerans is distinguished from m. marinum by the presence of a virulence plasmid which encodes a mac ... | 2006 | 16923788 |
| a sensitive fret probe assay for the selective detection of mycobacterium marinum in fish. | mycobacterium marinum is the causative agent of mycobacteriosis in wild and cultured fish and of atypical infection in humans. for the diagnosis of m. marinum, cultural and traditional polymerase chain reaction (pcr) methods are currently used. however, these protocols, although able to discriminate within mycobacterium spp., have proved to be time-consuming or difficult to carry out. for this reason, the aim of this study was to obtain a rapid and specific diagnostic tool to quantify fish mycob ... | 2010 | 19912457 |
| expanded range and new host species of mycobacterium shottsii and m. pseudoshottsii. | mycobacterium shottsii and m. pseudoshottsii are recently described mycobacteria commonly isolated from chesapeake bay striped bass morone saxatilis. however, their distribution in striped bass outside of the chesapeake region and their ability to infect alternative hosts have not been described. mycobacteria identified as m. shottsii (based on fatty acid methyl ester analysis and multigene sequencing) were isolated from striped bass collected in albemarle sound, north carolina, and white perch ... | 2009 | 20043404 |
| quantitative pcr assay for mycobacterium pseudoshottsii and mycobacterium shottsii and application to environmental samples and fishes from the chesapeake bay. | striped bass (morone saxatilis) in the chesapeake bay are currently experiencing a very high prevalence of mycobacteriosis associated with newly described mycobacterium species, mycobacterium pseudoshottsii and m. shottsii. the ecology of these mycobacteria outside the striped bass host is currently unknown. in this work, we developed quantitative real-time pcr assays for m. pseudoshottsii and m. shottsii and applied these assays to dna extracts from chesapeake bay water and sediment samples, as ... | 2010 | 20656856 |
| nineteen cases of buruli ulcer diagnosed in japan from 1980 to 2010. | the etiology, clinical manifestations, and treatment of 19 sporadic cases of buruli ulcer (bu) in japan are described. the cases originated in different regions of honshu island, with no evidence of patient contact with an aquatic environment. the majority (73.7%) of cases occurred in females, with an average age of 39.1 years for females and 56.8 years for males. all patients developed ulcers on exposed areas of the skin (e.g., face, extremities). most ulcers were <5 cm in diameter (category i) ... | 2011 | 21880966 |
| Mycobacterium pseudoshottsii isolated from 24 farmed fishes in western Japan. | Mycobacteria isolated from epizootics of farmed fishes in western Japan were examined for the first time using multi-genotypic analysis. By analysis of the sequences of the internal transcribed spacer between the 16S and 23S rRNA genes (ITS) region and the partial 16S rRNA, hsp65, and rpoB genes, M. pseudoshottsii was identified as the causative agent in these infections. Prior to this study, only M. marinum has been known as the causative agent of lethal mycobacterial disease in marine fishes i ... | 2011 | 21986278 |
| internalization of mycobacterium shottsii and mycobacterium pseudoshottsii by acanthamoeba polyphaga. | amoebae serve as environmental hosts to a variety of mycobacteria, including mycobacterium avium and mycobacterium marinum. mycobacterium shottsii and mycobacterium pseudoshottsii are waterborne species isolated from the spleens and dermal lesions of striped bass (morone saxatilis) from the chesapeake bay. the optimal growth temperature for these fish isolates is 25 °c. in the present study, amoebae were examined as a potential environmental reservoir for these fish pathogens. several studies de ... | 2013 | 23899000 |
| draft genome sequence of the fish pathogen mycobacterium pseudoshottsii strain jcm15466, a species closely related to m. marinum. | mycobacterium pseudoshottsii is a slowly growing photochromogenic mycobacterium and fish pathogen isolated from wild marine fishes. m. pseudoshottsii closely resembles m. marinum, which is a human and animal pathogen. here, we report the draft genome sequence of m. pseudoshottsii strain jcm15466, originally isolated from striped bass, morone saxatilis. | 2016 | 26868383 |