| complete genome sequence of the marine planctomycete pirellula sp. strain 1. | pirellula sp. strain 1 ("rhodopirellula baltica") is a marine representative of the globally distributed and environmentally important bacterial order planctomycetales. here we report the complete genome sequence of a member of this independent phylum. with 7.145 megabases, pirellula sp. strain 1 has the largest circular bacterial genome sequenced so far. the presence of all genes required for heterolactic acid fermentation, key genes for the interconversion of c1 compounds, and 110 sulfatases w ... | 2003 | 12835416 |
| novel protein domains and motifs in the marine planctomycete rhodopirellula baltica. | the planctomycetes are a phylum of bacteria that have a unique cell compartmentalisation and yeast-like budding cell division and peptidoglycan-less proteinaceous cell walls. we wished to further our understanding of these unique organisms at the molecular level by searching for conserved amino acid sequence motifs and domains in the proteins encoded by rhodopirellula baltica. using blast and single-linkage clustering, we have discovered several new protein domains and sequence motifs in this pl ... | 2004 | 15251216 |
| taxonomic heterogeneity within the planctomycetales as derived by dna-dna hybridization, description of rhodopirellula baltica gen. nov., sp. nov., transfer of pirellula marina to the genus blastopirellula gen. nov. as blastopirellula marina comb. nov. and emended description of the genus pirellula. | ninety-seven strains of budding bacteria originating from various aquatic habitats and morphologically resembling planctomycetes were investigated taxonomically. taxonomic differentiation was based on dna-dna hybridization, physiological properties and chemotaxonomic tests. nineteen hybridization groups, containing 79 of the tested strains, were established. eighteen strains, however, did not fit into any of these groups. rhodopirellula baltica gen. nov., sp. nov. is described, with strain sh 1t ... | 2004 | 15388712 |
| the transcriptional regulator pool of the marine bacterium rhodopirellula baltica sh 1t as revealed by whole genome comparisons. | rhodopirellula baltica (strain sh 1t) is a free-living marine representative of the phylogenetically independent and environmentally relevant phylum planctomycetes. little is known about the regulatory strategies of free-living bacteria with large (7.15 mb) genomes. therefore, a consistent, quantitative and qualitative description was produced by comparing r. baltica's transcriptional regulator pool with that of 123 publicly available bacterial genomes. the overall results are congruous with ear ... | 2005 | 15621430 |
| archaea-like genes for c1-transfer enzymes in planctomycetes: phylogenetic implications of their unexpected presence in this phylum. | the unexpected presence of archaea-like genes for tetrahydromethanopterin (h4mpt)-dependent enzymes in the completely sequence geiome of the aerobic marine planctomycete pirellula sp. strain 1 ("rhodopirellula baltica") and in the currently sequenced genome of the aerobic freshwater planctomycete gemmata obscuriglobus strain uqm2246 revives the discussion on the origin of these genes in the bacterial domain. we compared the genomic arrangement of these genes in planctomyetes and methylotrophic p ... | 2004 | 15693614 |
| growth phase dependent regulation of protein composition in rhodopirellula baltica. | growth phase dependent changes of protein composition in the marine bacterium rhodopirellula baltica were quantitatively monitored by applying the two-dimensional difference gel electrophoresis (2d dige) technology. the number of regulated proteins (fold changes in protein abundance > absolute value(2)) increased from early (10) to late stationary growth phase (179), with fold changes reaching maximal values of 40. about 110 of these regulated protein spots were analysed by maldi-tof-ms and iden ... | 2005 | 16011746 |
| proteomic analysis of carbohydrate catabolism and regulation in the marine bacterium rhodopirellula baltica. | the marine bacterium rhodopirellula baltica is a model organism for aerobic carbohydrate degradation in marine systems, where polysaccharides represent the dominant components of biomass. on the basis of the genome sequence and a 2-d map of soluble proteins, the central catabolic routes of r. baltica were reconstructed. almost all enzymes of glycolysis and tca cycle were identified. in addition, almost all enzymes of the oxidative branch of the pentose phosphate cycle were detected. this proteom ... | 2005 | 16127727 |
| towards the proteome of the marine bacterium rhodopirellula baltica: mapping the soluble proteins. | the marine bacterium rhodopirellula baltica, a member of the phylum planctomycetes, has distinct morphological properties and contributes to remineralization of biomass in the natural environment. on the basis of its recently determined complete genome we investigated its proteome by 2-de and established a reference 2-de gel for the soluble protein fraction. approximately 1000 protein spots were excised from a colloidal coomassie-stained gel (ph 4-7), analyzed by maldi-ms and identified by pmf. ... | 2005 | 16127728 |
| highly enantioselective sec-alkyl sulfatase activity of the marine planctomycete rhodopirellula baltica shows retention of configuration. | | 2005 | 16161167 |
| biocatalytic approaches for the quantitative production of single stereoisomers from racemates. | strategies for the chemoenzymatic transformation of a racemate into a single stereoisomeric product in quantitative yield have been developed. a range of industrially relevant alpha-hydroxycarboxylic acids was deracemized in a stepwise fashion via lipase-catalysed enantioselective o-acylation, followed by mandelate racemase-catalysed racemization of the remaining non-reacted substrate enantiomer. alternatively, aliphatic alpha-hydroxycarboxylic acids were enzymatically isomerized using whole res ... | 2006 | 16545098 |
| analysis of the first genome fragment from the marine sponge-associated, novel candidate phylum poribacteria by environmental genomics. | the novel candidate phylum poribacteria is specifically associated with several marine demosponge genera. because no representatives of poribacteria have been cultivated, an environmental genomic approach was used to gain insights into genomic properties and possibly physiological/functional features of this elusive candidate division. in a large-insert library harbouring an estimated 1.1 gb of microbial community dna from aplysina aerophoba, a poribacteria-positive 16s rrna gene locus was ident ... | 2006 | 16584473 |
| whole-genome reciprocal blast analysis reveals that planctomycetes do not share an unusually large number of genes with eukarya and archaea. | the genome sequences of rhodopirellula baltica, formerly pirellula sp. strain 1, blastopirellula marina, gemmata obscuriglobus, and kuenenia stuttgartiensis were used in a series of pairwise reciprocal best-hit analyses to evaluate the contested evolutionary position of planctomycetes. contrary to previous reports which suggested that r. baltica had a high percentage of genes with closest matches to archaea and eukarya, we show here that these planctomycetes do not share an unusually large numbe ... | 2006 | 17021241 |
| a novel anticoagulant protein with high affinity to blood coagulation factor va from tegillarca granosa. | a novel inhibitory protein against blood coagulation factor va (fva) was purified from muscle protein of granulated ark (tegillarca granosa, order arcoida, marine bivalvia) by consecutive fplc method using anion exchange and gel permeation chromatography. in the results of esiqtof tandem mass analysis and database research, it was revealed that the purified t. granosa anticoagulant protein (tgap) has 7.7 kda of molecular mass and its partial sequence, hthlqraphpnalgyhgk, has a high identity (64% ... | 2007 | 17927919 |
| expression, purification, crystallization and preliminary x-ray analysis of the polysaccharide lyase rb5312 from the marine planctomycete rhodopirellula baltica. | polysaccharide lyases belonging to family pl1 act on pectins. these anionic polymers are usually produced by terrestrial plants and therefore pectinolytic enzymes are not frequently observed in marine microorganisms. the protein rb5312 from the marine bacterium rhodopirellula baltica is distantly related to family pl1 pectate lyases, but its exact function is unclear. in this study, the expression and purification of a recombinant form of rb5312 are described. this protein was crystallized using ... | 2008 | 18323615 |
| detailed proteome analysis of growing cells of the planctomycete rhodopirellula baltica sh1t. | rhodopirellula baltica sh1(t), which was isolated from the water column of the kieler bight, a bay in the southwestern baltic sea, is a marine aerobic, heterotrophic representative of the ubiquitous bacterial phylum planctomycetes. we analyzed the r. baltica proteome by applying different preanalytical protein as well as peptide separation techniques (1-d and 2-de, hplc separation) prior to ms. that way, we could identify a total of 1115 nonredundant proteins from the intracellular proteome and ... | 2008 | 18340632 |
| an improved isolation method for attached-living planctomycetes of the genus rhodopirellula. | rhodopirellula baltica, an attached-living marine bacterium, was so far isolated as aerobic, heterotrophic bacterium forming pink-to-red colonies on ampicillin-containing plates. but many bacteria grow in the presence of ampicillin: marine samples from european seas contained on average 10,365 colony forming bacteria per ml water sample or sediment. therefore we developed an improved enrichment protocol to isolate rhodopirellula strains. to include attached-living bacteria, particles, plankton c ... | 2009 | 19303037 |
| biochemical characterization of haloalkane dehalogenases drba and dmbc, representatives of a novel subfamily. | this study focuses on two representatives of experimentally uncharacterized haloalkane dehalogenases from the subfamily hld-iii. we report biochemical characterization of the expression products of haloalkane dehalogenase genes drba from rhodopirellula baltica sh1 and dmbc from mycobacterium bovis 5033/66. the drba and dmbc enzymes show highly oligomeric structures and very low activities with typical substrates of haloalkane dehalogenases. | 2009 | 19502442 |
| detection and widespread distribution of the nrfa gene encoding nitrite reduction to ammonia, a short circuit in the biological nitrogen cycle that competes with denitrification. | degenerate primers to detect nrfa were designed by aligning six nrfa sequences including escherichia coli k-12, sulfurospirillum deleyianum and wolinella succinogenes. these primers amplified a 490 bp fragment of nrfa. the ability of these primers to detect nrfa was tested with chromosomal dna isolated from a variety of bacteria: they could distinguish between bacteria in which the gene is known to be present or absent. the positive reference organisms spanned the various classes of proteobacter ... | 2004 | 19712292 |
| transcriptional response of the model planctomycete rhodopirellula baltica sh1(t) to changing environmental conditions. | the marine model organism rhodopirellula baltica sh1(t) was the first planctomycete to have its genome completely sequenced. the genome analysis predicted a complex lifestyle and a variety of genetic opportunities to adapt to the marine environment. its adaptation to environmental stressors was studied by transcriptional profiling using a whole genome microarray. | 2009 | 19725962 |
| jane: efficient mapping of prokaryotic ests and variable length sequence reads on related template genomes. | ests or variable sequence reads can be available in prokaryotic studies well before a complete genome is known. use cases include (i) transcriptome studies or (ii) single cell sequencing of bacteria. without suitable software their further analysis and mapping would have to await finalization of the corresponding genome. | 2009 | 19943962 |
| determination of the diversity of rhodopirellula isolates from european seas by multilocus sequence analysis. | in the biogeography of microorganisms, the habitat size of an attached-living bacterium has never been investigated. we approached this theme with a multilocus sequence analysis (mlsa) study of new strains of rhodopirellula sp., an attached-living planctomycete. the development of an mlsa for rhodopirellula baltica enabled the characterization of the genetic diversity at the species level, beyond the resolution of the 16s rrna gene. the alleles of the nine housekeeping genes acsa, guaa, trpe, pu ... | 2010 | 19948850 |
| broad-spectrum antibiotic resistance of planctomycetes organisms determined by etest. | the in vitro susceptibility of planctomycetes organisms to antibiotics has seldom been studied and when it has, a variety of methods have been used. the objective of the study was to expand the knowledge of planctomycetes antibiotic susceptibility patterns. | 2010 | 20699245 |
| planctomycetes dominate biofilms on surfaces of the kelp laminaria hyperborea. | bacteria belonging to planctomycetes display several unique morphological and genetic features and are found in a wide variety of habitats on earth. their ecological roles in these habitats are still poorly understood. planctomycetes have previously been detected throughout the year on surfaces of the kelp laminaria hyperborea from southwestern norway. we aimed to make a detailed investigation of the abundance and phylogenetic diversity of planctomycetes inhabiting these kelp surfaces. | 2010 | 20950420 |
| life cycle analysis of the model organism rhodopirellula baltica sh 1(t) by transcriptome studies. | the marine organism rhodopirellula baltica is a representative of the globally distributed phylum planctomycetes whose members exhibit an intriguing lifestyle and cell morphology. the analysis of r. baltica's genome has revealed many biotechnologically promising features including a set of unique sulfatases and c1-metabolism genes. salt resistance and the potential for adhesion in the adult phase of the cell cycle were observed during cultivation. to promote the understanding of this model organ ... | 2010 | 21255355 |
| ammonium and attachment of rhodopirellula baltica. | a dimorphic life cycle has been described for the planctomycete rhodopirellula baltica sh1(t), with juvenile motile, free-swimming cells and adult sessile, attached-living cells. however, attachment as a response to environmental factors was not investigated. we studied the response of r. baltica to nitrogen limitation. in batch cultures, ammonium limitation coincided with a dominance of free-swimming cells and a low number of aggregates. flow cytometry revealed a quantitative shift with increas ... | 2011 | 21340506 |
| Planctomycetes diversity associated with macroalgae. | Planctomycetes associated with 12 macroalgae from the north coast of Portugal were isolated, using an improved method. A total of 138 isolates were found to comprise 10 operational taxonomic units (OTUs), with 65% of the strains being closely related to the species Rhodopirellula baltica. The other strains are probably new species or genera related to Rhodopirellula, Blastopirellula and Planctomyces. Some of the OTUs isolated are unique and have never been found before in previous studies. Catal ... | 2011 | 21726244 |
| bioinformatic analyses of integral membrane transport proteins encoded within the genome of the planctomycetes species, rhodopirellula baltica. | rhodopirellula baltica (r. baltica) is a planctomycete, known to have intracellular membranes. because of its unusual cell structure and ecological significance, we have conducted comprehensive analyses of its transmembrane transport proteins. the complete proteome of r. baltica was screened against the transporter classification database (tcdb) to identify recognizable integral membrane transport proteins. 342 proteins were identified with a high degree of confidence, and these fell into severa ... | 2013 | 23969110 |
| mannosylglucosylglycerate biosynthesis in the deep-branching phylum planctomycetes: characterization of the uncommon enzymes from rhodopirellula baltica. | the biosynthetic pathway for the rare compatible solute mannosylglucosylglycerate (mgg) accumulated by rhodopirellula baltica, a marine member of the phylum planctomycetes, has been elucidated. like one of the pathways used in the thermophilic bacterium petrotoga mobilis, it has genes coding for glucosyl-3-phosphoglycerate synthase (gpgs) and mannosylglucosyl-3-phosphoglycerate (mgpg) synthase (mgga). however, unlike ptg. mobilis, the mesophilic r. baltica uses a novel and very specific mgpg pho ... | 2013 | 23921581 |
| developing techniques for the utilization of planctomycetes as producers of bioactive molecules. | planctomycetes are conspicuous, ubiquitous, environmentally important bacteria. they can attach to various surfaces in aquatic habitats and form biofilms. their unique ftsz-independent budding cell division mechanism is associated with slow growth and doubling times from 6 h up to 1 month. despite this putative disadvantage in the struggle to colonize surfaces, planctomycetes are frequently associated with aquatic phototrophic organisms such as diatoms, cyanobacteria or kelp, whereby planctomyce ... | 2016 | 27594849 |
| identification of a mammalian-type phosphatidylglycerophosphate phosphatase in the eubacterium rhodopirellula baltica. | cardiolipin is a glycerophospholipid found predominantly in the mitochondrial membranes of eukaryotes and in bacterial membranes. cardiolipin interacts with protein complexes and plays pivotal roles in cellular energy metabolism, membrane dynamics, and stress responses. we recently identified the mitochondrial phosphatase, ptpmt1, as the enzyme that converts phosphatidylglycerolphosphate (pgp) to phosphatidylglycerol, a critical step in the de novo biosynthesis of cardiolipin. upon examination o ... | 2013 | 23293031 |
| heterologous carotenoid-biosynthetic enzymes: functional complementation and effects on carotenoid profiles in escherichia coli. | a limited number of carotenoid pathway genes from microbial sources have been studied for analyzing the pathway complementation in the heterologous host escherichia coli. in order to systematically investigate the functionality of carotenoid pathway enzymes in e. coli, the pathway genes of carotenogenic microorganisms (brevibacterium linens, corynebacterium glutamicum, rhodobacter sphaeroides, rhodobacter capsulatus, rhodopirellula baltica, and pantoea ananatis) were modified to form synthetic e ... | 2013 | 23144136 |
| a unique pool of compatible solutes on rhodopirellula baltica, member of the deep-branching phylum planctomycetes. | the intracellular accumulation of small organic solutes was described in the marine bacterium rhodopirellula baltica, which belongs to the globally distributed phylum planctomycetes whose members exhibit an intriguing lifestyle and cell morphology. sucrose, α-glutamate, trehalose and mannosylglucosylglycerate (mgg) are the main solutes involved in the osmoadaptation of r. baltica. the ratio and total intracellular organic solutes varied significantly in response to an increase in salinity, tempe ... | 2013 | 23826385 |
| internal loop photobiodegradation reactor (ilpbr) for accelerated degradation of sulfamethoxazole (smx). | the internal loop photobiodegradation reactor (ilpbr) was evaluated for the degradation of the pharmaceutical sulfamethoxazole (smx) using batch experiments following three protocols: photolysis alone (p), biodegradation alone (b), and intimately coupled photolysis and biodegradation (p&b). smx was removed more rapidly by p&b than by either p or b alone, and the corresponding dissolved organic carbon (doc) removals by p&b also were higher. the faster smx removal probably was due to a synergy bet ... | 2012 | 22215070 |
| the c-terminal regions of yidc from rhodopirellula baltica and oceanicaulis alexandrii bind to ribosomes and partially substitute for srp receptor function in escherichia coli. | the marine gram-negative bacteria rhodopirellula baltica and oceanicaulis alexandrii have, in contrast to escherichia coli, membrane insertases with extended positively charged c-terminal regions similar to the yidc homologues in mitochondria and gram-positive bacteria. we have found that chimeric forms of e. coli yidc fused to the c-terminal yidc regions from the marine bacteria mediate binding of yidc to ribosomes and therefore may have a functional role for targeting a nascent protein to the ... | 2014 | 24261830 |
| an overview of 2d dige analysis of marine (environmental) bacteria. | microbes are the "unseen majority" of living organisms on earth and main drivers of the biogeochemical cycles in marine and most other environments. their significance for an intact biosphere is bringing environmental bacteria increasingly into the focus of genome-based science. proteomics is playing a prominent role for providing a molecular understanding of how these microbes work and for identifying the key biocatalysts involved in the major biogeochemical processes. this overview describes t ... | 2012 | 22311773 |
| permanent draft genome of 'rhodopirellula islandica' strain k833. | the planctomycete strain k833 was isolated from cold waters at the coast of island and is tentatively named 'rhodopirellula islandica'. it has a lower temperature range for growth than other genome-sequenced rhodopirellula strains affiliating to rhodopirellula baltica and 'rhodopirellula europaea'. the permanent draft genome of strain k833 was obtained as part of a larger study on the biogeography of rhodopirellula species in european marine waters. the genome consists of 55 contigs with a genom ... | 2015 | 26319626 |
| roseimaritima ulvae gen. nov., sp. nov. and rubripirellula obstinata gen. nov., sp. nov. two novel planctomycetes isolated from the epiphytic community of macroalgae. | four isolates, belonging to the deep-branching phylum planctomycetes, were recovered from the biofilm of two marine macroalgae, ulva sp. and laminaria sp., from the northern coast of portugal. these strains were light pink- or red-pigmented; the cells were variable in shape and usually organized in rosettes. they had a dimorphic cell cycle with budding reproduction. the organisms were chemoheterotrophic, strictly aerobic and mesophilic. the 16s rrna gene sequence analysis showed that the strains ... | 2015 | 25487949 |
| permanent draft genome of rhodopirellula rubra swk7. | the genome of rhodopirellula rubra strain swk7 was sequenced as a permanent draft to complement the full genome sequence of the type strain rhodopirellula baltica sh1(t). this isolate is part of a larger study to infer the biogeography of rhodopirellula species in european marine waters, as well as to amend the genus description of r. baltica. this genomics resource article is the fourth among a series of five publications reporting in a total of eight new permanent draft genomes of rhodopirellu ... | 2014 | 24291061 |
| permanent draft genomes of the two rhodopirellula europaea strains 6c and sh398. | the genomes of two rhodopirellula europaea strains were sequenced as permanent drafts to study the genomic diversity within this genus, especially in comparison with the closed genome of the type strain rhodopirellula baltica sh1(t). the isolates are part of a larger study to infer the biogeography of rhodopirellula species in european marine waters, as well as to amend the genus description of r. baltica. this genomics resource article is the second of a series of five publications describing a ... | 2014 | 24286834 |
| permanent draft genomes of the rhodopirellula maiorica strain sm1. | the genome of rhodopirellula maiorica strain sm1 was sequenced as a permanent draft to complement the full genome sequence of the type strain rhodopirellula baltica sh1(t). this isolate is part of a larger study to infer the biogeography of rhodopirellula species in european marine waters, as well as to amend the genus description of r. baltica. this genomics resource article is the fifth of a series of five publications reporting in total eight new permanent daft genomes of rhodopirellula speci ... | 2014 | 24286833 |
| permanent draft genome of rhodopirellula sallentina sm41. | the genome of rhodopirellula sallentina sm41 was sequenced as a permanent draft to supplement the full genome sequence of the type strain rhodopirellula baltica sh1(t). this isolate is part of a larger study to gain insights into the biogeography of rhodopirellula species in european marine waters, as well as to amend the genus description of r. baltica. this genomics resource article is the third of a series of five publications reporting in total eight new permanent daft genomes of rhodopirell ... | 2014 | 24286832 |
| permanent draft genomes of the three rhodopirellula baltica strains sh28, swk14 and wh47. | the genomes of three rhodopirellula baltica strains were sequenced as permanent drafts to complement the full genome sequence of the type strain r. baltica sh1(t). the isolates are part of a larger study to infer the biogeography of rhodopirellula species in european marine waters, as well as to amend the genus description of r. baltica. this genomics resource article is the first of a series of five publications reporting in total eight new permanent daft genomes of rhodopirellula species. | 2014 | 24286831 |
| high ultraviolet c resistance of marine planctomycetes. | planctomycetes are bacteria with particular characteristics such as internal membrane systems encompassing intracellular compartments, proteinaceous cell walls, cell division by yeast-like budding and large genomes. these bacteria inhabit a wide range of habitats, including marine ecosystems, in which ultra-violet radiation has a potential harmful impact in living organisms. to evaluate the effect of ultra-violet c on the genome of several marine strains of planctomycetes, we developed an easy a ... | 2013 | 24052365 |
| diversity of planctomycetes in iron-hydroxide deposits from the arctic mid ocean ridge (amor) and description of bythopirellula goksoyri gen. nov., sp. nov., a novel planctomycete from deep sea iron-hydroxide deposits. | planctomycetes form a deep branching and distinct phylum of the domain bacteria, and represent a fascinating group due to their unusual features such as intracellular compartmentalization and lack of peptidoglycan in their cell walls. the phylum planctomycetes was described already in 1924, but still the diversity of this phylum represents an enigma and unexploited resource. in this study the diversity of the phylum planctomycetes in low temperature iron-hydroxide deposits at the mohns ridge, a ... | 2013 | 24018702 |
| from genome mining to phenotypic microarrays: planctomycetes as source for novel bioactive molecules. | most members of the phylum planctomycetes share many unusual traits that are unique for bacteria, since they divide independent of ftsz through asymmetric budding, possess a complex life cycle and comprise a compartmentalized cell plan. besides their complex cell biological features planctomycetes are environmentally important and play major roles in global matter fluxes. such features have been successfully employed in biotechnological applications such as the anaerobic oxidation of ammonium in ... | 2013 | 23982431 |
| genetic diversity of rhodopirellula strains. | rhodopirellula baltica sh1(t) is a marine planctomycete with 7,325 genes in its genome. ten strains of the genus rhodopirellula were studied in whole genome microarray experiments to assess the extent of their genetic relatedness to r. baltica sh1(t). dna of strains which were previously affiliated with the species r. baltica (otu a) hybridized with 3,645-5,728 genes of the type strain on the microarray. strains sh398 and 6c (otu b), representing a closely related species with an average nucleot ... | 2013 | 23975513 |
| rhodopirellula rosea sp. nov., a novel bacterium isolated from an ark clam scapharca broughtonii. | a novel gram-negative, motile, and ovoid-shaped strain, lhwp3(t), which belonged to the family planctomycetaceae in the phylum planctomycetes, was isolated from a dead ark clam scapharca broughtonii collected during a mass mortality event on the south coast of korea. phylogenetic analysis based on the 16s rrna gene sequences indicated that the isolate was most closely related to the type strain of rhodopirellula baltica, with a shared 16s rrna gene sequence similarity of 94.8%. the isolate grew ... | 2013 | 23812809 |
| expression of sulfatases in rhodopirellula baltica and the diversity of sulfatases in the genus rhodopirellula. | the whole genome sequence of rhodopirellula baltica sh1(t), published nearly 10years ago, already revealed a high amount of sulfatase genes. so far, little is known about the diversity and potential functions mediated by sulfatases in planctomycetes. we combined in vivo and in silico techniques to gain insights into the ecophysiology of planktomycetal sulfatases. comparative genomics of nine recently sequenced rhodopirellula strains detected 1120 open reading frames annotated as sulfatases (enzy ... | 2013 | 23273849 |
| cell surface proteome of the marine planctomycete rhodopirellula baltica. | the surface proteome (surfaceome) of the marine planctomycete rhodopirellula baltica sh1(t) was studied using a biotinylation and a proteinase k approach combined with sds-page and mass spectrometry. 52 of the proteins identified in both approaches could be assigned to the group of potential surface proteins. among them are some high molecular weight proteins, potentially involved in cell-cell attachment, that contain domains shown before to be typical for surface proteins like cadherin/dockerin ... | 2012 | 22623273 |
| isolation and characterization of planctomycetes from the sediments of a fish farm wastewater treatment tank. | the increasing ecological significance of planctomycetes and the still limited knowledge of this group prompted us to obtain cultured isolates from the sediment of a treatment water recycling tank of a marine fish farm. presence of strains from this group was assessed in the sediments and water column of the tank. eleven isolates were obtained from the sediment sample by exploiting planctomycetes natural resistance to several antibiotics and their capacity to degrade organic matter. based on mor ... | 2012 | 22622812 |
| determination of zeta potential in planctomycetes and its application in heavy metals toxicity assessment. | zeta potential of planctomycetes was evaluated under different environmental conditions and correlated to cell viability. phylogenetically distinct strains of the planctomycetes presented different negative zeta potential values. more negative values were associated with rhodopirellula spp. and related to the great amount of fimbriae in these species. milli-q water was chosen as the best dispersion media to perform the measurements. zeta potential increased with ionic strength and varied with ph ... | 2012 | 22576374 |
| matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for the identification of environmental organisms: the planctomycetes paradigm. | we have developed a matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms)-based identification technique for planctomycetes organisms, which are used here as bacteria of suitable diversity at genus and species level for testing resolution of the method. planctomyces maris atcc 29201, planctomyces brasiliensis atcc 49424(t) , p. brasiliensis atcc 49425, planctomyces limnophilus atcc 43296(t) , blastopirellula marina atcc 49069(t) , rhodopirellula baltica dsm ... | 2010 | 23766281 |