| studies on the mycoflora of aswan high dam lake, egypt: monthly variations. | fifty-one species and one variety appertaining to twenty one genera of mesophilic fungi were recovered from the monthly samples of marginal water (44 species, 1 variety and 18 genera) and submerged mud (78 species, 1 variety and 30 genera) of aswan high dam lake during the period from july 1985 to december 1986. the most common species were aspergillus fumigatus, a. flavus, a. terreus, a. niger and penicillium funiculosum. the highest fungal populations were almost detected either in october, in ... | 1990 | 2352135 |
| metabolism of ferulic and syringic acids by micromycetes. | the ability of 814 strains of micromycetes to grow on ferulic and syringic acids was investigated. after cultivation on solid media, 106 and 108 strains were selected and cultivated in liquid synthetic medium. chromatographic analysis allowed classification of fungi into different groups according to the consumption of phenolic compounds and the appearance of new metabolites. finally, paecilomyces variotii and pestalotia palmarum were chosen and cultivated in the presence of ferulic acid in two ... | 1990 | 2273981 |
| phylogenetic relationships of five species of aspergillus and related taxa as deduced by comparison of sequences of small subunit ribosomal rna. | the nucleotide sequences of the genes encoding the 18s rrna of aspergillus flavus, a. nidulans, a. terreus and a. niger were elucidated and aligned to the sequences of a. fumigatus. in addition, the 18s rrna sequences of the v4-v9 region of morphologically similar filamentous fungi, e.g. penicillium chrysogenum, p. marneffei and paecilomyces variotii, were elucidated. phylogenetic analysis and comparison showed a very close intergeneric relationship of the genus aspergillus to species of the gen ... | 1995 | 7666299 |
| hyalohyphomycosis by paecilomyces lilacinus in a renal transplant patient and a review of human paecilomyces species infections. | a case of hyalohyphomycosis caused by paecilomyces lilacinus is described in a renal transplant patient. infection was localized on the left forearm. two separate cultures of the lesions yielded the same organism. histological sections disclosed hyaline elements in the tissue. the infection responded well to therapy with oral griseofulvin (500 mg daily) and the patient was cured within 45 days. forty-six cases of human infection due to paecilomyces species have been reported previously in the li ... | 1990 | 2194015 |
| cornexistin: a new fungal metabolite with herbicidal activity. | cornexistin, a new compound demonstrating promising herbicidal activity, was purified from the culture filtrate of a newly-isolated fungus identified as paecilomyces variotii sank 21086. the compound was extracted with organic solvents from the culture filtrate, purified using column chromatography on sephadex lh-20 and finally crystallized from methylene chloride. following analysis of its physico-chemical properties it was identified to be a new compound belonging to the nonadride group. chemi ... | 1991 | 1955388 |
| a bold mold? paecilomyces variotii peritonitis during continuous ambulatory peritoneal dialysis. | a patient on peritoneal dialysis developed peritonitis due to paecilomyces variotii. it appears that this relatively rare organism may be starting to assert itself as a more important human pathogen. a plea is made for speciation. | 1991 | 1951344 |
| peritoneal eosinophilia associated with paecilomyces variotii infection in continuous ambulatory peritoneal dialysis. | a 65-year-old woman maintained on continuous ambulatory peritoneal dialysis (capd) presented with a 5-month history of intermittent cloudy bags and sterile peritoneal and peripheral blood eosinophilia, which failed to clear despite conventional antibiotics. impaired catheter inflow and delayed effluent drainage gradually occurred and intracatheter streptokinase, administered to rectify catheter dysfunction, dislodged a catheter cast composed of fungal hyphae of paecilomyces variotii. fungal peri ... | 1991 | 1951343 |
| contaminated commercial charcoal as a source of fungi in the respiratory tract. | to investigate the possibility that contaminated commercial activated charcoal may serve as a source for fungal colonization or infection of the lower respiratory tract. | 1991 | 1813580 |
| thermophilic and thermotolerant fungi of animals' hair. | nine thermophilic genera and 17 species in addition to one variety of aspergillus flavus, malbranchea pulchella and humicola grisea were collected from hair samples in riyadh, saudi arabia at 45 degrees c. fifty-one hair specimens of rabbit, sheep, camel and horse were examined for the presence of thermophilic fungi. the most frequent species were aspergillus fumigatus, aspergillus niger, thermoascus aurantiacus and malbranchea pulchella var. sulfurea. in low frequency, aspergillus flavus, asper ... | 1991 | 1805498 |
| relationships between exposure to spores from rhizopus microsporus and paecilomyces variotii and serum igg antibodies in wood trimmers. | a longitudinal study of wood trimmers exposed to mould spores was carried out from 1985 to 1988. exposure to airborne spores from rhizopus microsporus ssp. rhizopodiformis and paecilomyces variotii was measured by scanning electron microscopy of filter samples. specific igg antibodies to these moulds were measured by elisa in serum samples collected at half-year intervals. antibody levels to r. microsporus and p. variotii were higher in wood trimmers than in other sawmill workers whose jobs had ... | 1992 | 1597347 |
| sphingofungins e and f: novel serinepalmitoyl transferase inhibitors from paecilomyces variotii. | | 1992 | 1474000 |
| [the transformation of the saprotrophic mycelial form of the fungus paecilomyces variotii into the tissue parasitic form and its morphological characteristics]. | the fungus paecilomyces variotii is known to develop as a saprotrophic mycelial form in the soil. when penetrating into the human organism or placing into a medium containing cultured cells it transforms into a parasitic flesh form. this form of the fungus was shown to undergo 3 morphological stages: endospore, juvenile (intermediate) form and mature sphaerula. the transformation of the mycelial form into parasitic one occurs in the biological media containing cells of the host, inside or outsid ... | 1992 | 1435563 |
| a model for the combined effects of temperature and salt concentration on growth rate of food spoilage molds. | we modeled mold growth on a solid culture medium at various temperatures and nacl concentrations by using five common food spoilage molds (penicillium roqueforti, trichoderma harzianum, paecilomyces variotii, aspergillus niger, and emericella nidulans). for the description of the growth rate (expressed as the increase in colony diameter per unit of time) as a function of temperature and nacl concentration, a six-parameter model has been developed. the model combines either the rosso-type or the ... | 1997 | 9327540 |
| hyalohyphomycosis caused by paecilomyces variotii: a case report, animal pathogenicity and 'in vitro' sensitivity. | a case of cutaneous infection in a 25-year-old male caused by paecilomyces variotii is described. animal pathogenicity studies with normal and cortisone-treated mice revealed the predeliction of p. variotii for skin and liver in both normal and cortisone-treated mice and for lungs and heart only in immunosuppressed mice. 5-fluorocytosine gave the best mic value for p. variotii in vitro. this report documents for the first time that p. variotii causes cutaneous infection. | 1992 | 1416918 |
| chronic suppurative otitis media caused by paecilomyces variotii. | a case of chronic suppurative otitis media is described in a patient with a chronic cold and cough. the presenting symptoms were otorrhoea, perforation of the tympanic membrane and granulation in the air cells of the left mastoid cavity. x-ray showed sclerotic mastoid and partial cellular obliteration. histologic sections revealed aggregated fungal masses, filamentous hyphae and single-celled structures of paecilomyces variotii. the infection responded to topical ketoconazole therapy. this is th ... | 1995 | 7650580 |
| paecilomyces variotii contamination in the lumen of a saline-filled breast implant. | this report describes a case of gross contamination with the filamentous fungus p. variotii cultured from an intraluminal saline breast implant removed from a patient 14 months after implantation because of severe capsular contracture. we suspect the fungal contamination occurred when a container of saline was left open in the operating room prior to filling and placement of the implant. this case may be the first documented report of microbial growth and reproduction in the internal environment ... | 1995 | 7480245 |
| species-specific production of microbial volatile organic compounds (mvoc) by airborne fungi from a compost facility. | thirteen airborne fungal species frequently isolated in composting plants were screened for microbial volatile organic compounds (mvoc), i.e., aspergillus candidus, a. fumigatus, a. versicolor, emericella nidulans, paecilomyces variotii, penicillium brevicompactum, penicillium clavigerum, penicillium crustosum, penicillium cyclopium, penicillium expansum, penicillium glabrum, penicillium verruculosum, and tritirachium oryzae. air samples from pure cultures were sorbed on tenax gr and analyzed by ... | 1999 | 10448558 |
| cerebrospinal fluid shunt colonization and obstruction by paecilomyces variotii. case report. | a 57-year-old woman underwent ventriculoperitoneal shunt placement for noncommunicating hydrocephalus. she required several shunt revisions over a 2-year period for recurrent hydrocephalus. the shunt was subsequently found to be obstructed by growth of the saprophytic fungus, paecilomyces variotii, an infrequent human pathogen. paecilomyces infections have caused complications associated with prosthetic cardiac valves and synthetic lens implantation; this is the first reported association with a ... | 1981 | 7192726 |
| paecilomyces pyelonephritis complicating nephrolithiasis and review of paecilomyces infections. | we report a case of paecilomyces variotii isolated from the renal pelvis at ureterolithotomy. the patient presented with nephrolithiasis, acute flank pain, fever and pyuria, which resolved postoperatively. paecilomyces has infected the cornea, prosthetic lens implants, lacrimal sac, maxillary sinuses, prosthetic mitral and aortic valves, skin and a ventriculoperitoneal shunt. | 1983 | 6684177 |
| the tolerance and nutritional value of two microfungal foods in human subjects. | the tolerance of human subjects to two microfungal food products was studied in separate double-blind cross-over studies. as an addition to the subject's usual diets, cookies with and without 20 g of a product from fusarium graminearium were fed to a group of 100 individuals daily. in a second study, cupcakes with and without 10 g of paecilomyces variotii were given daily to 50 individuals. mild rashes possibly related to one of the microfungal food products occurred in two individuals fed p var ... | 1984 | 6547808 |
| [biomass production and biological purification of distillation slops in a 2-step process]. | in order to lower the chemical demand (cod) of slops from cane molasses alcohol a treatment of two steps which allows the production of single cell protein of candida utilis and paecilomyces variotii has been performed. its use reduces the treatment cost. in the first step the slops without sterilization supplemented with ammonium sulphate (5 g.l-1) and dipotassium phosphate (0.5 g.l-1) was inoculated with c. utilis and p. variotii. the yield was 24 and 18 g.l-1 of dry biomass and cod reduction ... | 1983 | 6400760 |
| detection of fungi in cerebrospinal fluid. | with the exception of cryptococcus neoformans, fungi are rarely detected in cerebrospinal fluid obtained from patients having or suspected of having fungal meningitis. a review of the literature reveals that several fungi have been either isolated, observed, or both in cerebrospinal fluid specimens. these fungi include acremonium species, aspergillus amstelodami, a. flavus, a. fumigatus, a. oryzae, a. terreus, blastomyces dermatitidis, candida albicans, c. tropicalis, c. viswanathii, coccidioide ... | 1983 | 6349340 |
| systemic paecilomycosis in a dog. | disseminated paecilomycosis was diagnosed in an adult dog without underlying immunosuppressive disease. during the 3-month illness (before euthanasia), the dog had ulcerative granulomatous inguinal lymphadenitis, fever, anorexia, dyspnea, generalized lymphadenopathy, retinochoroiditis, and seizures. fungal organisms isolated from inguinal and prescapular lymph nodes before the dog was euthanatized were identified histologically. paecilomyces variotii was isolated from the prescapular lymph node ... | 1987 | 3654321 |
| fungi associated with urea-formaldehyde foam insulation in canada. | sixty-eight fungal taxa were identified from samples of urea-formaldehyde foam insulation taken from canadian residences. mesophilic taxa were predominant, with penicillium spp., trichoderma harzianum and paecilomyces variotii observed most frequently. extensive or conspicuous growth also was seen for hormoconis resinae, stachybotrys chartarum and trichoderma viride in some samples. the potential for these fungi to have contributed to the adverse health effects reported in some homes containing ... | 1987 | 3627222 |
| paecilomyces variotii. an unusual cause of isolated sphenoid sinusitis. | isolated fungal sphenoid sinusitis is an unusual head and neck infection. we describe the first reported case (to our knowledge) of paecilomyces variotii sphenoid sinusitis. in addition, it represents one of the few reported cases of isolated fungal sphenoid sinusitis. diagnosis and management of this condition are described. aggressive surgical management is advocated when fungal sphenoid sinusitis is suspected. | 1988 | 3355698 |
| water relations of paecilomyces variotii, eurotium amstelodami, aspergillus candidus and aspergillus sydowii, xerophilic fungi isolated from indonesian dried fish. | the water relations of four xerotolerant fungi, paecilomyces variotii, eurotium amstelodami, aspergillus candidus and aspergillus sydowii, isolated from dried salt fish, were examined at 25 degrees c, on media in which water activity (aw) was controlled by nacl or a glucose/fructose mixture. all fungi were less tolerant of nacl than glucose/fructose at low aw. p. variotii grew 2 to 3 times faster on glucose/fructose media than on nacl. the minimum aw permitting germination varied from 0.753 for ... | 1988 | 3275312 |
| [paecilomyces infection]. | the clinical and experimental studies showed that etiology and pathogenesis of allergic alveolitis in the inhabitants of the samarkand region stem from mycosepsis caused by paecilomyces variotii bainier, that is immunocomplex affection of the small and middle vessels and cell-mediated granulomatous inflammation of the tissues. | 1989 | 2530574 |
| species-specific profiles of mycotoxins produced in cultures and associated with conidia of airborne fungi derived from biowaste. | the potential to produce mycotoxins and non-volatile secondary metabolites was investigated for approximately 250 freshly isolated fungal strains. among the eleven most relevant species, viz. aspergillus flavus, a. fumigatus, a. niger, a. parasiticus, a. versicolor, emericella nidulans, paecilomyces variotii, penicillium brevicompactum, p. clavigerum, p. crustosum, and p. polonicum, a wide range of metabolites partly of toxicological relevance was identified. several unknown metabolites were fou ... | 2000 | 11109562 |
| a cardinal model to describe the effect of water activity on the growth of moulds. | a simple model was proposed to describe the effect of water activity (aw) on the radial growth rate of moulds. this model is deduced from the cardinal model family proposed by rosso in 1995, which is only defined from cardinal values of environmental factors (minimum, optimum and maximum values), the growth rate observed at the optimal value of the environmental factors, and n, a shape parameter. for aw, a simple form of cardinal model is proposed. this form is obtained for n = 2 and aw(max) = 1 ... | 2001 | 11246910 |
| influence of formic acid on fungal flora of barley and on aflatoxin production in aspergillus flavus link. | in recent years aspergillus flavus and aflatoxin production have been noted on several occasions in grain preserved with formic acid. samples of mouldy barley treated with formic acid and stored in an open bin were investigated for the presence of fungi. in the lower part of the bin there was a clear dominance of fusarium sporotrichioides, and deoxynivalenol and neosolaniol were detected. a. flavus and a. fumigatus were also present. paecilomyces variotii occurred, almost as a pure culture, in t ... | 1989 | 2515436 |
| bacereutin, an antifungal antibiotic isolated from metabolites of bacillus cereus chu 130. | an antifungal metabolite, bacereutin, was isolated from culture filtrate of bacillus cereus chu 130. the bacterium was isolated from soils collected in changhwa county, taiwan, and was grown in soybean meal-mannitol broth for production of the antibiotic metabolite. the antibiotic metabolite was isolated by adsorption column chromatography of amberite xad-2 and was purified by passing through the chromatographic columns packed with dowex 50w-x8, sephadex lh 20 and biogel p-2. the antibiotic meta ... | 1989 | 2484044 |
| serum igg antibodies to mold spores in two norwegian sawmill populations: relationship to respiratory and other work-related symptoms. | wood trimmers and planing operators from two separate sawmill populations (n = 303 and 170) were studied by serology assessment and a self-administered questionnaire. igg antibodies to rhizopus microsporus ssp. rhizopodiformis, paecilomyces variotii, and aspergillus fumigatus were measured by elisa. the questionnaire included questions about general respiratory symptoms and symptoms after handling moldy timber. personal exposure of wood trimmers to mold spores and wood dust was measured in one p ... | 1993 | 8213847 |
| microbial, chemical and physical aspects of citrus waste composting. | citrus waste supplemented with calcium hydroxide and with a c/n ratio of 24:1, ph of 6.3 and moisture content of 60% was composted by piling under shelter. with regular turning over of the pile and replenishment of moisture, the thermic phase lasted for 65-70 days and composting was completed after 3 months. compost thus prepared had an air-filled porosity of 14%, water-holding capacity of 590 ml l(-1), bulk density of 1.05 g cm(-3) and conductivity of 480 ms m(-1). phosphorus content (in mg l(- ... | 2002 | 11708757 |
| growing incidence of cutaneous and ungual infections by non-dermatophyte fungi at jabalpur (m.p.). | a random survey of skin and nail infections was done at jabalpur (m.p.). out of the 80 suspected cases 60 were culture positive. out of these 25 were of dermatophyte infection followed by 21 cases of phaeohyphomycosis, 7 of aspergillosis, 4 of hyalohyphomycosis, 2 of mixed infection in which a non-dermatophyte was associated with a dermatophyte and 1 had yeast infection. trichophyton rubrum was the only dermatophyte isolated. among the non-dermatophyte infections cases of alternaria chlamydospor ... | 1993 | 8276472 |
| paecilomyces variotii in peritoneal dialysate. | four cases of peritonitis caused by the filamentous fungus paecilomyces variotii in patients on continuous ambulatory peritoneal dialysis are reported. removal of the tenckhoff catheter and antifungal chemotherapy led to resolution of symptoms in all cases. possible contaminating events are discussed, and reported infections with p. variotii are reviewed. | 1993 | 8408561 |
| hyalohyphomycosis caused by paecilomyces variotii in an obstetrical patient. | a case of hyalohyphomycosis, caused by paecilomyces variotii, has been described in a 31-year-old female, who had undergone a cesarean section in her 39th week of pregnancy for a trial of labour. five days following delivery, she complained of sharp, cramp-like pains, localized to the incisional site. she became febrile (38.2 degrees c). an ultrasound examination revealed a complex mass and fluid within the pelvis and upper abdomen. the fluid was drained by a needle aspiration and the patient wa ... | 1996 | 8817175 |
| paecilomyces variotii contamination in the lumen of a saline-filled breast implant. | | 1996 | 8942937 |
| specificity of a sandwich enzyme-linked immunosorbent assay for detecting aspergillus galactomannan. | the specificity of a sandwich enzyme-linked immunosorbent assay (elisa) for detecting aspergillus galactomannan was tested with exoantigens of 29 fungi cultured from clinical specimens. cross-reactivity was observed with penicillium chrysogenum, penicillium digitatum, and paecilomyces variotii. furthermore, 40 serum samples obtained from bacteremic patients with hematologic malignancies were retrospectively tested by sandwich elisa. false-positive reactions with the serum were reproducible but d ... | 1997 | 8968919 |
| associated mycoflora of rye bread. | penicillium roqueforti (27%), p. corylophilum (20%) and eurotium species (15%) made up the important mycoflora associated with rye bread on 3425 identified fungi isolates. these fungi were dominant as spoilers of packaged rye bread in almost every month of a 4 year investigation. penicillium decumbens (3%), paecilomyces variotti (8%) and aspergillus flavus (5%) were found more rarely, but were the major species found over periods of a few months. penicillium commune (5%), p. solitum (4%), a. nig ... | 1996 | 8987693 |
| microbial growth inside saline-filled breast implants. | in vitro and in vivo experiments were conducted to determine whether intraluminal saline in breast implants can support the growth of common wound-infecting microorganisms over a prolonged period of time. the bacteria tested were staphylococcus aureus, staphylococcus epidermidis, escherichia coli, corynebacterium jeikeium, enterobacter cloacae, klebsiella pneumoniae, and pseudomonas aeruginosa. three fungal species also were tested: aspergillus fumigatus, paecilomyces variotii, and candida albic ... | 1997 | 9207676 |
| in vitro antifungal activity of pneumocandin l-743,872 against a variety of clinically important molds. | the in vitro activity of the new antifungal drug pneumocandin l-743,872 against 55 isolates of clinically important molds was examined by an adapted macrobroth dilution method for yeasts. pneumocandin l-743,872 exhibited in vitro antifungal activity against alternaria sp., aspergillus flavus, aspergillus fumigatus, curvularia lunata, exophiala jeanselmei, fonsecaea pedrosoi, paecilomyces variotii, and scedosporium apiospermum. the drug appeared to lack significant in vitro inhibitory activity ag ... | 1997 | 9257774 |
| endogenous fungal endophthalmitis caused by paecilomyces variotii. | | 1999 | 10396397 |
| modelling the effect of ethanol on growth rate of food spoilage moulds. | the effect of ethanol (e) on the radial growth rate (mu) of food spoilage moulds (aspergillus candidus, aspergillus flavus, aspergillus niger, cladosporium cladosporioides, eurotium herbariorum, mucor circinelloides, mucor racemosus, paecilomyces variotii, penicillium chrysogenum, penicillium digitatum, rhizopus oryzae and trichoderma harzianum) was assessed in potato dextrose agar (pda) medium at a(w) 0.99, 25 degrees c. in order to model this effect, the monod type equation described previousl ... | 2005 | 15698687 |
| galactomannans from the cell walls of species of paecilomyces sect. paecilomyces and their teleomorphs as immunotaxonomic markers. | an alkali-extractable and water-soluble fraction (f1s) was obtained from cell walls of paecilomyces variotii and species of the related genera talaromyces, byssochlamys and thermoascus. the structure of the main polysaccharide of these fractions was studied and found to consist of a core of (1 --> 6)-alpha-mannopyranose partially substituted at 0.2 by chains of galactofuranose and shorter chains of mannopyranose. the differences in the regularity of the branching points and the length of the gal ... | 1999 | 10537200 |
| microbial populations, ammonification and nitrification in soil treated with urea and inorganic salts. | soil fertilization with urea at rates of 0.2 and 0.5 mg n/g soil was toxic for total counts of bacteria and fungi except with cellulolytic fungi where growth was promoted by addition of urea after 90-d incubation. also, the population numbers of both bacteria and fungi were significantly decreased when soil was amended with cacl2 and k2so4 applied at two levels (50 and 100 mumol/g soil). some alleviation of the toxic effect of either urea or the inorganic salts was observed when they were applie ... | 1999 | 10588055 |
| characterization of a novel, antifungal, chitin-binding protein from streptomyces tendae tü901 that interferes with growth polarity. | the afp1 gene, which encodes the antifungal protein afp1, was cloned from nikkomycin-producing streptomyces tendae tü901, using a nikkomycin-negative mutant as a host and screening transformants for antifungal activity against paecilomyces variotii in agar diffusion assays. the 384-bp afp1 gene has a low g+c content (63%) and a transcription termination structure with a poly(t) region, unusual attributes for streptomyces genes. afp1 was purified from culture filtrate of s. tendae carrying the af ... | 1999 | 10601197 |
| overview of spa-s-843 in vitro activity against filamentous fungi. | in this study, we investigated the in vitro antifungal activity of a new water-soluble partricin a derivative, n-dimethylaminoacetyl-partricin a 2-dimethylaminoethylamide diascorbate, coded spa-843, currently developed by società prodotti antibiotici. the activity of spa-s-843 was compared to that of amphotericin b against 13 strains of aspergillus spp., 4 strains of mucor sp., 4 strains of rhizopus oryzae, 2 strains paecilomyces variotii, 5 strains of penicillium spp., 1 strain of sporothrix sc ... | 2000 | 10601795 |
| [melanin pigments of the fungi paecilomyces variotii and aspergillus carbonarius]. | pigments synthesized by micromycetes paecilomyces variotii and aspergillus carbonarius are true melanins. copper ions and bicyclic phenolic compounds stimulated melaninogenesis, whereas benzotriazole inhibited this process. precursors of melanin pigments were obtained and identified. p-hydroxybenzoic acid was shown to be the main product of melanin degradation. melanins of these fungi are concluded to belong to the dihydronaphthalene group. | 2000 | 10780001 |
| paecilomyces variotii peritonitis in an infant on automated peritoneal dialysis. | fungal peritonitis is a serious complication of chronic peritoneal dialysis (cpd) and is frequently associated with cpd drop-out. paecilomyces variotii, a common saprophytic fungus, rarely causes human infection. to date, only nine adult or adolescent patients with p. variotii peritonitis during continuous ambulatory peritoneal dialysis have been reported. in all patients, successful treatment required antifungal therapy and removal of the peritoneal catheter. we report the first case of p. vari ... | 2000 | 10805461 |
| quality control and reference guidelines for clsi broth microdilution susceptibility method (m 38-a document) for amphotericin b, itraconazole, posaconazole, and voriconazole. | although standard conditions are available for testing the susceptibilities of filamentous fungi to antifungal agents by the clinical and laboratory standards institute (clsi; formerly national committee for clinical laboratory standards) broth microdilution assay, quality control (qc) mic limits have not been established for any mold-agent combination. this multicenter (eight-center) study documented the reproducibility of tests for one isolate of paecilomyces variotii atcc mya-3630 and 11 othe ... | 2005 | 16207990 |
| paecilomyces variotii fungemia in a patient with multiple myeloma: case report and literature review. | infectious complications are common in patient with multiple myeloma. however, paecilomyces variotii, a common saprophytic fungus, rarely causes human infection. we report the first case of p. variotii fungemia in this illness with good response with adapted anti-mycotic treatment. | 2005 | 16230212 |
| voriconazole-resistant disseminated paecilomyces variotii infection in a neutropenic patient with leukaemia on voriconazole prophylaxis. | paecilomyces variotii, an emerging hyalohyphomycetes, has been reported to be susceptible in vitro to voriconazole. we describe a case of disseminated p. variotii infection in a neutropenic child with relapsed leukaemia who was on voriconazole prophylaxis. the p. variotii isolate was resistant to voriconazole in vitro. the patient responded to liposomal amphotericin b. | 2005 | 16291275 |
| streptomyces halstedii k122 produces the antifungal compounds bafilomycin b1 and c1. | streptomyces halstedii k122 was previously found to produce antifungal compounds on solid substrates that inhibit radial growth of fungi among ascomycetes, basidiomycetes, deuteromycetes, oomycetes, and zygomycetes, and strongly affected hyphal branching and morphology. during growth of s. halstedii k122 in submerged culture, no antifungal activity could be detected. however, cultivation of s. halstedii in thin (1 mm) liquid substrate layers in large surface-area tissue culture flasks caused int ... | 2000 | 10941524 |
| in vitro susceptibility testing of filamentous fungi: comparison of etest and reference microdilution methods for determining itraconazole mics. | the performance of the etest for itraconazole susceptibility testing of 50 isolates of filamentous fungi was assessed in comparison with the national committee for clinical laboratory standards (nccls) proposed standard microdilution broth method. the nccls method employed rpmi 1640 broth medium, and mics were read after incubation for 48 h at 35 degrees c. etest mics were determined with rpmi agar containing 2% glucose and with casitone agar and were read after incubation for 24 h (aspergillus ... | 2000 | 10970383 |
| an outbreak of pulmonary mycosis in respiratory burst-deficient (gp91(phox-/-))mice with concurrent acidophilic macrophage pneumonia. | an outbreak of pulmonary abscessation due to the common environmental fungus paecilomyces variotii occurred in a colony of gp91(phox-/-)mice, which lack functional nadph oxidase, the enzyme complex responsible for generating the respiratory burst in phagocytes. parenchyma surrounding the abscesses showed intense acidophilic macrophage pneumonia, and similar but much milder lesions were present in each of 24 gp91(phox-/-)mice free from other pulmonary lesions. these findings indicate a high preva ... | 2000 | 11032674 |
| paecilomyces variotii in a pediatric patient with lung transplantation. | aspergillus has been noted to be the most common species of filamentous fungus isolated from the airways of lung transplantation (tx) patients. in general, the bronchi are colonized asymptomatically with aspergillus but this places such a patient population at greater risk of invasive infection. other filamentous fungal species may also assume importance in this patient population. here we report the post-transplant isolation of paecilomyces variotii from the airways of a pediatric patient with ... | 2000 | 11079275 |
| antifungal and antibacterial activities of araucaria araucana (mol.) k. koch heartwood lignans. | five lignans (secoisolariciresinol, pinoresinol, eudesmin, lariciresinol, and lariciresinol-4-methyl ether) were isolated from an meoh extract from araucaria araucana (mol.) k. koch wood for the first time in this species and their structures determined with spectroscopic methods. the antimicrobial activities of these compounds were determined for the bacteria citrobacter sp., bacillus subtilis, escherichia coli, micrococcus luteus, staphylococcus aureus, and pseudomonas aeruginosa, and for the ... | 2006 | 16610214 |
| deterioration and spoilage of peanuts and desiccated coconuts from two sub-saharan tropical east african countries due to the associated mycobiota and their degradative enzymes. | a broad variety of fungi (84 species belonging to 36 genera) were identified with more taxa infesting peanut seed samples from two tropical countries (29 genera and 61 species) compared to those found in desiccated coconuts (20 genera and 55 species) on both drbc and dg18 media. this may be due to the higher moisture levels in peanuts (5.07-7.97%) compared with coconuts (1.5-4.17%). more taxa and propagules were recovered on dg18 in both cases. the dominant fungi from both substrates on both iso ... | 2001 | 11407493 |
| fungal flora of the digestive tract of 5 species of triatomines vectors of trypanosoma cruzi, chagas 1909. | a study of the mycobiota in the digestive tract of 5 important species of triatomines, triatoma brasiliensis, t infestans, t. sordida, t. pseudomaculata and t. vitticeps, was made. the digestive tracts of 164 adults and 535 nymphs of those triatomines were studied and 393 fungal strains were isolated. the genera with the greatest number of species were penicillium (19 species), aspergillus (17 species) and acremonium (5 species) and the most frequent species, in decreasing order, were penicilliu ... | 2001 | 11502063 |
| air sampling of fungal spores on filters. an investigation on passive sampling and viability. | in this study, glycerol was tested as a collection substrate for passive bioaerosol sampling. filters (mixed cellulose acetate and nitrate) were soaked in glycerol and exposed for an aerosol from three different fungal species: penicillum commune, aspergillus versicolor and paecilomyces variotii. the passive sampling method was compared with a closed-face polycarbonate filter sampling method. exposure was performed in an exposure chamber. the total number of spores was determined by microscopic ... | 1999 | 11529137 |
| [the nature of melanin pigments from some micro- and macromycetes]. | new inhibitors of melanin formation by micromycetes aspergillus carbonarius, alternaria alternata, and paecilomyces variotii and basidiomycetes inonotus obliquus and phellinus robustus were found. precursors of melanin pigments were isolated and identified. p-hydroxybenzoic acid was identified among the products of alkaline degradation of melanin formed by micromycetes, whereas in the case of macromycetes this was protocatechuic acid. therefore, melanins of the former were found to belong to the ... | 2002 | 12068581 |
| unexplained increase in paecilomyces variotii blood culture isolates in the uk. | | 2006 | 17213552 |
| delayed sternotomy wound infection due to paecilomyces variotii in a lung transplant recipient. | we report a case of sternotomy wound infection caused by paecilomyces variotii in a previously bronchiectatic patient, occurring 10 months after bilateral sequential lung transplantation. the use of prophylactic antifungal therapy, the persistent colonization with paecilomyces, and sternal instability after clamshell incision may have contributed to the development of delayed deep sternal wound infection. besides antifungal therapy, vigorous surgical debridement is vital for treatment success. w ... | 2002 | 12398880 |
| paecilomyces variotii central nervous system infection in a patient with cancer. | paecilomyces variotii was isolated from two subsequent cerebrospinal fluid (csf) specimens of a cancer patient. identification was confirmed through beta-tubulin and rdna its sequencing. mics were determined for seven antifungal agents; the isolate was found to be susceptible to amphotericin b (amb), itraconazole (itz), ketaconazole (ktz) and 5-fluorocytosine (5fc) but resistant to fluconazole (flz) and miconazole (mcz). despite antimycotic therapy, the infection proved to be fatal. | 2003 | 12588483 |
| paecilomyces peritonitis: case report and review of the literature. | while filamentous fungi are a rare cause of peritonitis in peritoneal dialysis patients, there is increasing recognition of paecilomyces species as pathogens in such patients. we herein report a case of fungal peritonitis secondary to the filamentous paecilomyces variotii species. the patient had a long and ultimately fatal course of illness despite catheter removal, discontinuation of peritoneal dialysis, recurrent intraabdominal abscess drainage, and prolonged courses of antifungal therapy. ou ... | 2003 | 12708573 |
| purification and biochemical characterization of a thermostable extracellular glucoamylase produced by the thermotolerant fungus paecilomyces variotii. | an extracellular glucoamylase produced by paecilomyces variotii was purified using deae-cellulose ion exchange chromatography and sephadex g-100 gel filtration. the purified protein migrated as a single band in 7% page and 8% sds-page. the estimated molecular mass was 86.5 kda (sds-page). optima of temperature and ph were 55 degrees c and 5.0, respectively. in the absence of substrate the purified glucoamylase was stable for 1 h at 50 and 55 degrees c, with a t (50) of 45 min at 60 degrees c. th ... | 2008 | 17938981 |
| use of response surface methodology for selection of nutrient levels for culturing paecilomyces variotii in eucalyptus hemicellulosic hydrolyzate. | eucalyptus hemicellulose was hydrolyzed by treating eucalyptus wood chips with sulfuric acid. the hydrolyzate was used as the substrate to produce single-cell protein by growing paecilomyces variotii ioc-3764 for 72 or 96 h. the influences of rice bran, ammonium sulfate and fermentation time were verified by a 23 full-factorial central composite design. at the optimum process conditions, the cell concentration was 12.06 g/l, which was obtained when the microorganisms were cultivated for 89 h in ... | 2003 | 12733574 |
| fungal endophthalmitis caused by paecilomyces variotii following cataract surgery: a presumed operating room air-conditioning system contamination. | to report a case of delayed fungal endophthalmitis by paecilomyces variotii following uncomplicated cataract surgery. to our knowledge this is the first reported case of postoperative endophthalmitis by this species. | 2004 | 15043549 |
| in vitro interactions of approved and novel drugs against paecilomyces spp. | we have evaluated the in vitro activity of 15 combinations of antifungal drugs (amphotericin b, itraconazole, voriconazole, albaconazole, ravuconazole, terbinafine, and micafungin) against four isolates of paecilomyces variotii and three of p. lilacinus. the interaction of terbinafine with the four azoles was synergistic for 53% of the combinations, while the interactions of both amphotericin b and micafungin with the rest of antifungal agents were mainly indifferent. | 2004 | 15215136 |
| sexual reproduction as the cause of heat resistance in the food spoilage fungus byssochlamys spectabilis (anamorph paecilomyces variotii). | paecilomyces variotii is a common cosmopolitan species that is able to spoil various food- and feedstuffs and is frequently encountered in heat-treated products. however, isolates from heat-treated products rarely form ascospores. in this study we examined by using molecular techniques and mating tests whether this species can undergo a sexual cycle and form ascospores. the population structure of this species was examined by analyzing the nuclear ribosomal internal transcribed spacer 1 (its1) a ... | 2008 | 18192427 |
| saprophytic and cycloheximide resistant fungi isolated from golden hamster. | healthy hair samples from golden hamsters were examined for the presence of dermatophytes and non-dermatophytes using baiting technique and direct inoculation. thirty-four species and 2 varieties attributed to 17 genera were recovered. paecilomyces variotii (isolated from 84.4% of the examined hair) and aspergillus niger (81.3%) were the more frequent isolates on sabouraud's dextrose agar (sda) without cycloheximide. our results have clearly demonstrated that the hair of hamster was free from tr ... | 1998 | 9768288 |
| determination of itraconazole and hydroxy-itraconazole in sera using high-performance-liquid-chromatography and a bioassay. | the concentrations of itraconazole and its active metabolite, hydroxy-itraconazole, were determined in the sera of 49 patients having received itraconazole for therapeutic or prophylactic purposes. measurements by hplc were compared with a biological assay using a strain of paecilomyces variotii as test organism and itraconazole as standard. the concentration of hydroxy-itraconazole in serum was on average 1.6 times higher than the concentration of itraconazole. the sum of itraconazole and hydro ... | 1995 | 9810671 |
| biodegradation of toluene by the new fungal isolates paecilomyces variotii and exophiala oligosperma. | two new fungal strains, namely paecilomyces variotii and exophiala oligosperma, were isolated on toluene as the sole carbon and energy source, mineralizing the substrate into carbon dioxide. fungal strains isolated so far on such a pollutant and completely degrading it are very scarce. both fungi degraded the pollutant over the ph range 3.9-6.9 and temperature range 23-40 degrees c, but e. oligosperma was barely active at the highest temperature of 40 degrees c. fungal growth on alkylbenzenes at ... | 2005 | 15702332 |
| favolon b, a new triterpenoid isolated from the chilean mycena sp. strain 96180. | a new biologically active triterpenoid, favolon b (1), was isolated from fermentation broths of mycena sp. strain 96180. favolon b showed antifungal activities towards botrytis cinerea, mucor miehei, paecilomyces variotii and penicillium notatum. no activities were observed against bacteria and yeasts. the structure of favolon b was elucidated by spectroscopic techniques. | 2005 | 15813182 |
| the production and characterization of monoclonal antibodies to the fungus aspergillus versicolor. | fungal exposure measurements in indoor environments require accurate and precise monitoring methods. such techniques may be based on monoclonal antibodies (mabs) and enzyme-linked immunosorbent assays (elisa) and here we report the cross-reactivity patterns of mabs produced against aspergillus versicolor. balb/c mice were immunized with the particulate fraction of homogenized spores and 46 mabs (35 igm, nine igg3, two igg1) were produced and tested for cross-reactivity against 55 fungal species. ... | 2005 | 15910525 |
| evaluation of quantitative pcr and culture methods for detection of house dust fungi and streptomycetes in relation to moisture damage of the house. | microbial concentrations in vacuumed house dust samples (n = 71) were analysed by culture and quantitative polymerase chain reaction (qpcr) methods and their association with extent of moisture damage in the house was studied. | 2008 | 19241524 |
| correlation of biological activity and reactor performance in biofiltration of toluene with the fungus paecilomyces variotii cbs115145. | a biofiltration system inoculated with the mold paecilomyces variotii cbs115145 showed a toluene elimination capacity (ec) of around 250 g/m3 of biofilter/h, which was higher than the values usually reported for bacteria. p. variotii assimilated m- and p-cresols but not the o isomer. initial toluene hydroxylation occurred both on the methyl group and through the p-cresol pathway. these results were corroborated by detecting benzyl alcohol, benzaldehyde, and p-cresol as volatile intermediates. in ... | 2005 | 16085815 |
| purification and characterization of tannase from paecilomyces variotii: hydrolysis of tannic acid using immobilized tannase. | an extracellular tannase (tannin acyl hydrolase) was isolated from paecilomyces variotii and purified from cell-free culture filtrate using ammonium sulfate precipitation followed by ion exchange and gel filtration chromatography. fractional precipitation of the culture filtrate with ammonium sulfate yielded 78.7% with 13.6-folds purification, and diethylaminoethyl-cellulose column chromatography and gel filtration showed 19.4-folds and 30.5-folds purifications, respectively. molecular mass of t ... | 2006 | 16133325 |
| successful treatment of paecilomyces variotii splenic abscesses: a rare complication in a previously unrecognized chronic granulomatous disease child. | a case of splenic abscesses caused by paecilomyces variotii in a 21-month-old child with previously undiagnosed chronic granulomatous disease (cgd) is described. an x-linked form of cgd with deficiency of gp91-phox was diagnosed. the fungal pathogen was isolated from the abscess contents. cure was achieved by a partial splenectomy and a prolonged fluconazole and flucytosine combination treatment regimen. p. variotii is an emerging fungus in children with cgd. every effort should be made to recov ... | 2005 | 16168619 |
| metabolism of ferulic acid by paecilomyces variotii and pestalotia palmarum. | ferulic acid metabolism was studied in cultures of two micromycetes producing different amounts of phenol oxidases. in cultures of the low phenol oxidase producer paecilomyces variotii, ferulic acid was decarboxylated to 4-vinylguaiacol, which was converted to vanillin and then either oxidized to vanillic acid or reduced to vanillyl alcohol. vanillic acid underwent simultaneously an oxidative decarboxylation to methoxyhydroquinone and a nonoxidative decarboxylation to guaiacol. methoxyhydroquino ... | 1989 | 16348018 |
| biotransformation of p-coumaric acid by paecilomyces variotii. | to investigate the biotransformation of p-coumaric acid into p-hydroxybenzoic acid (p-hba) by paecilomyces variotii bainier mtcc 6581. | 2006 | 16411917 |
| identification of volatile metabolites from five fungal species cultivated on two media. | five fungal species, aspergillus versicolor, penicillium commune, cladosporium cladosporioides, paecilomyces variotii, and phialophora fastigiata, were cultivated on two media, malt extract agar and dichloran glycerol agar. culture flasks provided with inlet and outlet tubes were used and purified, and humidified air was constantly led through the flasks. air samples from the cultures were sorbed on tenax gr and analyzed by thermal desorption-gas chromatography. the produced volatile metabolites ... | 1995 | 16535095 |
| in vitro efficacy of a buffered chelating solution as an antimicrobial potentiator for antifungal drugs against fungal pathogens obtained from horses with mycotic keratitis. | to determine whether a novel third-generation chelating agent (8 mm disodium edta dehydrate and 20 mm 2-amino-2-hydroxymethyl-1, 3-propanediol) would act as an antimicrobial potentiator to enhance in vitro activity of antifungal medications against fungal isolates obtained from horses with mycotic keratitis. | 2006 | 16579746 |
| characterization of filamentous fungi isolated from moroccan olive and olive cake: toxinogenic potential of aspergillus strains. | during the 2003 and 2004 olive oil production campaigns in morocco, 136 samples from spoiled olive and olive cake were analyzed and 285 strains were isolated in pure culture. strains included 167 mesophilic strains belonging to ten genera: penicillium, aspergillus, geotrichum, mucor, rhizopus, trichoderma, alternaria, acremonium, humicola, ulocladium as well as 118 thermophilic strains isolated in 2003 and 2004, mainly belonging to six species: aspergillus fumigatus, paecilomyces variotii, mucor ... | 2006 | 16715545 |
| conversion of sinapic acid to syringic acid by a filamentous fungus paecilomyces variotii. | | 2006 | 16778358 |
| tannase production by paecilomyces variotii. | surface response methodology was applied to the optimization of the laboratory scale production of tannase using a lineage of paecilomyces variotii. a preliminary study was conducted to evaluate the effects of variables, including temperature ( degrees c), residue (%) (coffee husk:wheat bran), tannic acid (%) and salt solutions (%) on the production of tannase during 3, 5 and 7 days of fermentation. among these variables, temperature, residues and tannic acid had significant effects on tannase p ... | 2007 | 17045475 |
| a new rapid technique for screening the potential of implanted microorganisms to tolerate and grow on petroleum oily sludges. | standard experimental protocols for biodegrading petroleum hydrocarbons employ microbiological and/or analytical analysis, and require advanced machines and specialized professionals to carry them out. furthermore, the obtained results involve a high margin of error. in this paper, a new experimental technique was developed to facilitate the study of microbial capability to degrade petroleum oily sludges. the technique is based on growing microorganisms on 0.22 microm filter membranes laid over ... | 2006 | 17144260 |
| fermentation and antimicrobial activities of extracts from different species of fungus belonging to genus, trichoderma. | the present paper discusses the effects of the crude extracts of trichoderma spp. on a number of fungal and bacterial organisms. these include paecilomyces variotii, penicillium notatum, nematospora corylii, mucor miehei, bacillus brevis, bacillus subtilis, enterobacter dissolvens and sarcina lutea. the culture broth extracts of different isolates of trichoderma harzianum, t. longibrachiatum and t. koningii cultured in different media were investigated individually for in-vitro antifungal and an ... | 2004 | 17298115 |
| quality control and reference guidelines for clsi broth microdilution method (m38-a document) for susceptibility testing of anidulafungin against molds. | the clsi (formerly nccls) m38-a document for antifungal susceptibility testing of filamentous fungi does not describe guidelines for echinocandins. a multicenter study (eight centers) evaluated inter- and intralaboratory reproducibilities of two reading times (24 and 48 h or 48 and 72 h) and two end points (mics and minimum effective concentrations [mecs]) for evaluating anidulafungin against molds. anidulafungin mics (>or=50% inhibition) and mecs (morphological hyphal changes) were determined f ... | 2007 | 17475760 |
| [lung infection by paecilomyces variotii in a patient with breast cancer]. | | 2007 | 17927941 |
| purification and characterization of alcohol oxidase from paecilomyces variotii isolated as a formaldehyde-resistant fungus. | paecilomyces variotii iri017 was isolated as a formaldehyde-resistant fungus from wastewater containing formaldehyde. the fungus grew in a medium containing 0.5% formaldehyde and had consumed formaldehyde completely after 5 days. alcohol oxidase was purified from the fungus grown on methanol. a 20-fold purification was achieved with a yield of 44%. the molecular mass of the purified enzyme was estimated to be 73 and 450 kda by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and gel fil ... | 2008 | 17985128 |
| combinations of antimycotics to inhibit the growth of molds capable of producing 1,3-pentadiene. | some species of molds are capable of degrading sorbic acid to produce 1,3-pentadiene, a volatile compound with an unpleasant hydrocarbon-like odor. the effectiveness of reduced concentrations of sorbate, in combination with other antimycotics, to control the growth of these molds has not been described. we did a study to evaluate potassium sorbate, sodium benzoate, calcium propionate, disodium ethylenediaminetetraacetic acid (edta), and natamycin, alone and in combination, for their effectivenes ... | 2008 | 17993388 |
| quantifying fungal viability in air and water samples using quantitative pcr after treatment with propidium monoazide (pma). | a method is described to discriminate between live and dead cells of the infectious fungi aspergillus fumigatus, aspergillus flavus, aspergillus terreus, mucor racemosus, rhizopus stolonifer and paecilomyces variotii. to test the method, conidial suspensions were heat inactivated at 85 degrees c or held at 5 degrees c (controls) for 1 h. polycarbonate filters (25 mm diameter, 0.8 microm pore size) were placed on "welled" slides (14 mm diameter) and the filters treated with either pbs or pma. pro ... | 2008 | 18160156 |
| prevalence of allergic sensitization to indoor fungi in west virginia. | exposure to indoor fungi is of growing concern in residential and occupational environments in the united states. the purpose of this study was to determine the prevalence of sensitization to common indoor fungal species in an atopic population. we evaluated 102 patients (73 female and 29 male patients) for immunoglobulin e (ige) reactivity to a panel of skin-prick test (spt) reagents used for routine allergy testing. patients also were tested for six additional fungi that are common indoor cont ... | 2008 | 18302835 |
| airway toxicity of house dust and its fungal composition. | house dust is an important source of different toxic metabolites as well as allergens, including those of fungal origin, in the indoor environment. a bio-assay employing 1-day-old chick tracheas was used to characterize airway effects of 2-butanone and dimethylsulphoxide (me2so) extracts of 23 dust samples collected from water damaged (13) and control (10) danish schools. direct microscopical analysis of samples, followed by cultivation on dichloran 18 % glycerol agar at 25 degrees c for 10 days ... | 2004 | 15236501 |
| biofiltration of waste gases with the fungi exophiala oligosperma and paecilomyces variotii. | two biofilters fed toluene-polluted air were inoculated with new fungal isolates of either exophiala oligosperma or paecilomyces variotii, while a third bioreactor was inoculated with a defined consortium composed of both fungi and a co-culture of a pseudomonas strain and a bacillus strain. elimination capacities of 77 g m(-3) h(-1) and 55 g m(-3) h(-1) were reached in the fungal biofilters (with removal efficiencies exceeding 99%) in the case of, respectively, e. oligosperma and paecilomyces va ... | 2005 | 15647938 |
| generalized calcinosis cutis associated with disseminated paecilomycosis in a dog. | a 4-year-old spayed female mixed breed dog was referred to the michigan state university, veterinary teaching hospital (msu-vth) with vomiting, lethargy and anorexia of 2 weeks duration. abdominal radiographs and ultrasonography showed hepatosplenomegaly. cytological evaluation of ultrasound-guided fine needle aspirates of the liver and spleen revealed fungal organisms and pyogranulomatous inflammation; fungal culture documented paecilomyces variotii infection. the dog received antifungal therap ... | 2008 | 19037918 |
| allantofuranone, a new antifungal antibiotic from allantophomopsis lycopodina ibwf58b-05a. | in a screening for new bioactive compounds, the extract of allantophomopsis lycopodina strain ibwf58b-05a, an imperfect ascomycete, was found to exhibit strong but rather selective antibiotic activity against paecilomyces variotii. the bioactivity-guided isolation yielded allantofuranone, a new and uncommon gamma-lactone. this compound showed antifungal activity against p. variotii and penicillium species. this paper describes the isolation, structure elucidation and biological characteristics o ... | 2009 | 19198630 |
| activation of the promoter of the fengycin synthetase operon by the up element. | bacillus subtilis f29-3 produces an antifungal peptidic antibiotic that is synthesized nonribosomally by fengycin synthetases. our previous work established that the promoter of the fengycin synthetase operon is located 86 nucleotides upstream of the translational initiation codon of fenc. this investigation involved transcriptional fusions with a dna fragment that contains the region between positions -105 and +80 and determined that deleting the region between positions -55 and -42 reduces the ... | 2009 | 19447911 |
| microbial content of house dust samples determined with qpcr. | this study was designed to produce information about microbial concentrations using qpcr and their variation in different seasons and home environments with analyses of two types of house dust samples. also the correlations between the two types of samples and the reproducibility of the parallel subsamples were studied. two types of vacuumed house dust samples, rug dust and vacuum cleaner bag dust, were collected in 5 normal urban homes in four different seasons (n=20+20). from all dust samples, ... | 2009 | 19473690 |