| isolation of acholeplasma granularum from porcine faeces. | | 1976 | 945929 |
| [mycoplasms of the swine--a review]. | the mycoplasmas constitute a group of microorganisms placed between bacteria and virus. the name, mycoplasma, is derived from the mycelial morphology of the organisms. the minimal reproductive unit, the elementary body, measures 0.2-0.5 mum. unlike bacteria, mycoplasmas are not confined by a rigid cell wall, but just by a thin membrane. for their cultivation, though common bacteriological technique is adequate, especially enriched media are required. antibiotics, as a rule penicillins, are added ... | 1975 | 1153279 |
| preparation of mycoplasma hyopneumoniae antigen for the enzyme-linked immunosorbent assay. | methods of preparation of mycoplasma hyopneumoniae antigens for the enzyme-linked immunosorbent assay to detect specific antibody, and properties of the antigens, are described. the reactivity and specificity of antigen prepared by sephacryl s-300 column chromatography after treatment of m. hyopneumoniae cells with tween 20 (s-300 antigen) were superior to those of antigen prepared by sephadex g-25 column chromatography after treatment with tween 20, or to lipid antigen. there were no difference ... | 1989 | 2523756 |
| [generation of pro-coagulation activity by leukocytes stimulated in vitro with lipoglycans from mycoplasmas]. | lipoglycans represent a special type of lipopoly-saccharide that differs in structure from the well-known gram-negative bacteria lipopolysaccharide. after briefly describing their most important characteristics, the authors take into consideration the in vitro interaction between lipoglycans from acholeplasma granularum, acholeplasma oculi, and acholeplasma axanthum and human leukocytes in terms of production of procoagulant activity. the results obtained show that the examined lipoglycans posse ... | 1985 | 3843073 |
| physiological and serological comparisons among strains of mycoplasma granularum and mycoplasma laidlawii. | mycoplasma granularum strains grew on a medium devoid of animal serum or of serum fractions containing sterols; all strains possessed properties, including carotenoid biosynthesis, similar to those described for m. laidlawii. some common antigenic components were noted among m. granularum and m. laidlawii strains by indirect fluorescent-antibody tests. the growth of m. granularum strains was slightly inhibited by antiserum to m. laidlawii pg-8, and the electrophoretic patterns of cell proteins o ... | 1968 | 4870272 |
| the passive haemagglutination test for th detection of mycoplasma suipneumoniae and the possible diagnosis of enzootic pneumonia of pigs. | fourteen cases of enzootic pneumonia, nearly all of which had presented diagnostic difficulties using the metabolic-inhibition test, were re-examined using specific pig antisera in the passive haemagglutination test (pha). all proved positive for mycoplasma suipneumoniae, indicating that the test, used in this manner, might be particularly valuable for routine diagnosis.the pha test was also used to demonstrate antibody to m. suipneumoniae in pneumonic tissue and the associated bronchial lymph n ... | 1970 | 4988048 |
| heterogeneity among strains of mycoplasma granularum and identification of mycoplasma hyosynoviae, sp. n. | twelve filtrable, pleomorphic organisms isolated from swine joints and respiratory tracts had typical colonial and microscopic characteristics of mycoplasmas. they resisted penicillin and did not revert to cell wall-producing bacterial forms in media devoid of bacterial inhibitors. the morphological and growth characteristics of these mycoplasmas were similar to those described previously for mycoplasma granularum. however, a new name, m. hyosynoviae, is proposed for them since they differed bio ... | 1970 | 4991614 |
| intrapulmonary lymphoid tissue of pigs exposed to aerosols of carbon particles, of salmonella oranienburg, of mycoplasma granularum, and to an oral inoculum of larvae of metastrongylus apri. | | 1971 | 5090884 |
| [serologic differentiation between acholeplasma laidlawii (mycoplasma laidlawii) and acholeplasma granularum (mycoplasma granularum) strains using agglutination and precipitation]. | | 1971 | 5169114 |
| mycoplasma granularum of swine origin as a tissue culture contaminant. | | 1966 | 5342691 |
| isolation and characterization of the sheep erythrocyte receptor for acholeplasmal lipoglycans. | a receptor specific for lipoglycans from acholeplasma axanthum and acholeplasma granularum was isolated from sheep erythrocyte stroma by extraction with n-pentanol and permeation chromatography. the purified receptor appeared as one band on sodium dodecyl sulfate-polyacrylamide gels and stained with coomassie blue, periodate-schiff reagent, and sudan black. it was distinct from the erythrocyte receptor for gram-negative lipopolysaccharides and the glycophorin receptor for certain species of myco ... | 1982 | 6185420 |
| identification of the major antigenic determinants on lipoglycans from acholeplasma granularum and acholeplasma axanthum. | the major antigenic determinants of the lipoglycans from acholeplasma granularum and acholeplasma axanthum were found to be the oligosaccharide sequences glcp(beta 1 leads to 2)-glcp(alpha 1 leads to 4)-glcp and glcp(beta 1 leads to 2)-glcp(beta 1 leads to 2)-glcp, respectively. the disaccharides sophorose and maltose inhibited hemagglutination by specific antiserum of sheep erythrocytes coated with lipoglycan from a. granularum. only sophorose possessed this capacity with the lipoglycan from a. ... | 1983 | 6188700 |
| emmy klieneberger-nobel award lecture: the protein fingerprints of mycoplasmas. | the proteins of 91 strains of mycoplasma from 19 established species and three groups of uncertain taxonomic position were separated by two-dimensional polyacrylamide gel electrophoresis. considerable variations in protein patterns were found among strains from the same species or subspecies; the percentage of matching spots (% of congruence) ranged from 42%-100%, but many proteins were strongly conserved in all strains of a given species. maps of these conserved proteins could be used to identi ... | 1982 | 6750757 |
| tylosin tartrate and tiamutilin effects on experimental piglet pneumonia induced with pneumonic pig lung homogenate containing mycoplasmas, bacteria and viruses. | the effects of tylosin tartrate and tiamutilin were examined in pneumonias induced experimentally in neonatal piglets with a homogenate of pneumonic pig lung, obtained from pigs with naturally acquired enzootic pneumonia. the homogenate contained mycoplasmas, including mycoplasma hyopneumoniae (m suipneumoniae) and m hyorhinis, and certain bacteria and viruses. the experimental pneumonias generally resembled mycoplasmal pneumonia histologically but were complicated by aspiration pneumonia in som ... | 1982 | 7134653 |
| respiration-associated components of mollicutes. | no cytochrome pigments were detected by difference (reduced minus oxidized) spectroscopy at liquid nitrogen temperature in whole-cell preparations or membrane fractions of acholeplasma axanthum s273, acholeplasma equifetale n93, acholeplasma granularum bts39, acholeplasma laidlawii b-pg9, acholeplasma modicum pg-49, acholeplasma oculi 19l, mycoplasma arginini g230, mycoplasma arthritidis 07, mycoplasma pneumoniae fh, and mycoplasma pulmonis jb. all ten mollicutes species examined contained iron ... | 1981 | 7240087 |
| isolation and identification of mycoplasmas from pig lungs in singapore. | a total of 656 lung specimens comprising 196 grossly pneumonic lungs from clinically diseased pigs and 230 grossly pneumonic and normal lungs from abattoirs were cultured for mycoplasmas. mycoplasmas or acholeplasmas were recovered from 102 lung specimens, of which 28 were serologically identified (by disc growth inhibition and agar gel diffusion tests) as mycoplasma suipneumonia, 14 as m hyorhinis, 35 as acholeplasma granularum and 4 as a laidlawii. in addition, 15 isolates were unclassified an ... | 1981 | 7257151 |
| structure of the oligosaccharide chain of lipoglycan from acholeplasma granularum. | the membrane associated lipoglycan from acholeplasma granularum is a linear oligosaccharide attached to a diacylglycerol. the polymer has a monomeric weight of 20000 and is composed of glucose, galactose, n-acetylglucosamine, n-acetylfucosamine, glycerol and fatty acid esters. the proposed structure of the oligosaccharide chain is 12 repeating units of 9 sugars: glcp(beta 1 leads to 2)-glcp(alpha 1 leads to 4)-glcp(alpha 1 leads to 3,4)-fcnac(beta 1 leads to 3)-galp(alpha 1 leads to 3)-galp(alph ... | 1981 | 7284419 |
| an aldehydophosphoglycolipid from acholeplasma granularum. | a novel phosphoglycolipid and a triglucosyl diacylglycerol were found among the lipids of acholeplasma granularum. the tentative structure of the phosphoglycolipid was determined to be a phosphotriester, [beta-d-glucopyranosyl-(1 leads to 1)-x-glycerol-3-o-] [d-glyceraldehyde-3-o-] [1,2-diacyl-x-glycerol-3-o-kojibiosyl-6-o-]phosphate. the structure assigned to the triglucosyl lipid was beta-d-glucopyranosyl-(1 leads to 3)-alpha-d-glucopyranosyl-(1 leads to 2)-alpha-d-glucopyranosyl-(1 leads to 1 ... | 1980 | 7370288 |
| endotoxin-like activities of mycoplasmal lipopolysaccharides (lipoglycans). | lipoglycans (previously designated lipopolysaccharides) from several species of acholeplasma and from thermoplasma acidophilum were examined for endotoxin-like activities as measured by the standard rabbit fever test and the limulus amoebocyte lysate assay. the lipoglycans from acholeplasma granularum, achloplasma laidlawii, acholeplasma modicum, and acholeplasma oculi caused a febrile response at concentrations of 1 ng/ml per kg or greater, whereas with control escherichia coli ec-2 lipopolysac ... | 1980 | 7429642 |
| antiserum raised against gyrase a of acholeplasma laidlawii reacts with phytoplasma proteins. | part of the gyrase a gene (gyra) of acholeplasma laidlawii was cloned and incorporated directly downstream from a 6 x his tag segment of the pqe expression vector. the 23-kda fusion protein was expressed as a 6 x his-tagged protein in escherichia coli. the fusion protein was purified and used as an antigen for rabbit immunization. western immunoblot analysis revealed that the antiserum raised against the gyrase a fragment had a specific affinity for a 108-kda protein of a. laidlawii and cross-re ... | 2002 | 11814658 |