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diversity and phylogeny of plant growth-promoting bacilli from moderately acidic soil.the molecular diversity of aerobic endospore-forming bacteria, typically bacillus and its derived genera, has been investigated in various environments. however, there have been few investigations concerning bacillus in acidic soils. in this study, the genotypic diversity and phylogenetic relationships among plant growth-promoting (pgp) bacilli isolated from the rice rhizosphere growing in acidic soils of kerala (ph varying from 6.3 to 6.8) were investigated. for assessing their biocontrol poten ...201021077114
thermotolerant bacillus kokeshiiformis sp. nov. isolated from marine animal resources compost.a novel gram-staining-positive, endospore-forming, rod-shaped, facultatively anaerobic, thermotolerant bacterium, designated strain mo-04(t), was isolated from a marine animal resources (mar) compost. the 16s rrna gene sequence of strain mo-04(t) showed 99.4% similarity with bacillus thermolactis r-6488(t), 94.1% similarity with bacillus thermoamylovorans cncm i-1378(t), 93.3% similarity with bacillus humi lmg 22167(t), 93.2% similarity with bacillus niacini ifo 15566(t) and the similarities wit ...201424835163
isolation of thermophilic l-lactic acid producing bacteria showing homo-fermentative manner under high aeration condition.by applying non-sterile open fermentation of food waste, various thermotolerant l-lactic acid-producing bacteria were isolated and identified. the predominant bacterial isolates showing higher accumulation of l-lactic acid belong to 3 groups of bacillus coagulans, according to their 16s rrna gene sequence similarities. b. coagulans strains m21 and m36 produced high amounts of l-lactic acid of high optical purity and lactic acid selectivity in model kitchen refuse medium and glucose-yeast extract ...201424119530
development of a systematic feedback isolation approach for targeted strains from mixed culture systems.elucidation of functions of bacteria in a mixed culture system (mcs) such as composting, activated sludge system is difficult, since the system is complicating with many unisolated bacteria. here, we developed a systematic feedback isolation strategy for the isolation and rapid screening of multiple targeted strains from mcs. six major strains (corynebacterium sphenisci, bacillus thermocloacae, bacillus thermoamylovorans, bacillus smithii, bacillus humi, and bacillus coagulans), which are detect ...201727570222
bacillus luteolus sp. nov., a halotolerant bacterium isolated from a salt field.a novel gram-stain-positive, motile, strictly aerobic bacterium, designated yim 93174(t), was isolated from a salt field in korea. cells of this strain were rod-shaped and formed pale tangerine colonies and grew at ph 6.0-8.0 (optimal growth at ph 7.0), at 15-45 °c (optimum 28-37 °c) and at salinities of 0-10 % (w/v) nacl (optimum 0-2 % nacl). some phenotypic characters allowed differentiation of strain yim 93174(t) from its nearest phylogenetic relatives. comparative 16s rrna gene sequence anal ...201020584813
culture-independent analysis of microbial succession during composting of swine slurry and mushroom cultural wastes.bacterial diversity and the composition of individual communities during the composting process of swine and mushroom cultural wastes in a field-scale composter (hazaka system) were examined using a pcr-based approach. the composting process was divided into six stages based on recorded temperature changes. phylogenetic analysis of eighty 16s rrna sequences from uncultured composting bacterial groups revealed the presence of representatives from three divisions, including plant pathogenic bacter ...200819131687
bacillus alkalitelluris sp. nov., an alkaliphilic bacterium isolated from sandy soil.a gram-positive, alkaliphilic bacterium, designated strain ba288(t), was isolated from sandy soil. cells were facultatively anaerobic, endospore-forming rods that were motile by means of peritrichous flagella. the strain grew at 15-40 degrees c and ph 7.0-11.0 (optimally at 30 degrees c and ph 9.0-9.5) and at salinities of 0-4 % (w/v) nacl. phylogenetic analyses based on 16s rrna gene sequences showed that strain ba288(t) belonged to the genus bacillus and that bacillus herbersteinensis d-1,5a(t ...200818984705
bacillus arenosi sp. nov., bacillus arvi sp. nov. and bacillus humi sp. nov., isolated from soil.a group of nine gram-positive endospore-forming bacteria was isolated from soil of the drentse a agricultural research area in the netherlands. using (gtg)5-pcr genomic fingerprinting and fatty acid analysis, the nine isolates were divided into three consistent groups. on the basis of 16s rrna gene sequence similarity of representative strains, the nine isolates were shown to belong to the genus bacillus. the first group of four isolates was most closely related to bacillus carboniphilus (95.5 % ...200515653863
bacillus nitroreducens sp. nov., a humus-reducing bacterium isolated from a compost.a gram-staining-positive, facultative anaerobic, motile and rod-shaped bacterium, designated gss08(t), was isolated from a windrow compost pile and characterized by means of a polyphasic approach. growth occurred with 0-4 % (w/v) nacl (optimum 1 %), at ph 6.5-9.5 (optimum ph 7.5) and at 20-45 °c (optimum 37 °c). anaerobic growth occurred with anthraquinone-2,6-disulphonate, fumarate and no3 (-) as electron acceptor. the main respiratory quinone was mk-7. the predominant polar lipids were diphosp ...201626832132
bacillus pervagus sp. nov. and bacillus andreesenii sp. nov., isolated from a composting reactor.two strains, 8-4-e12(t) and 8-4-e13(t), were isolated from a biowaste composting reactor. based on 16s rrna gene sequences, both strains belong to the genus bacillus. strain 8-4-e12(t) was most closely related to the type strains of bacillus shackletonii, b. acidicola, b. sporothermodurans and b. oleronius (96.4, 96.3, 96.0 and 95.6 % 16s rrna gene similarity, respectively), whereas strain 8-4-e13(t) was most closely related to the type strain of bacillus humi (96.5 % sequence similarity). strai ...201424021730
bacillus onubensis sp. nov., isolated from the air of two andalusian caves.two gram-positive, catalase-positive, oxidase-negative, motile, endospore-forming, rod-shaped bacteria, designated as 0911mar22v3t and 0911tes10j4, were isolated from air samples collected in two show caves, located in andalusia, southern spain. phylogenetic analysis based on 16s rrna gene sequences indicated that both strains were indistinguishable and they were most closely related to bacillus humi dsm 16318t (98%). dna-dna hybridization values of the strain 0911mar22v3t with respect to strain ...201829395538
draft genome sequence of bacillus humi lmg 22167t (dsm 16318), an endospore-forming bacterium isolated from soil.bacillus humi lmg 22167(t) is a gram-positive, aerobic, and spore-forming bacterium here, we report the 4.80-mb draft genome sequence of b. humi lmg 22167(t), which is the first genome sequence of this species and will promote its fundamental research.201626847898
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