| suppressor of rna silencing encoded by the monopartite tomato leaf curl java begomovirus. | we previously isolated the monopartite begomovirus tomato leaf curl java virus (tolcjav) and satellite dnabeta02 from the same naturally infected tomato source in indonesia. tolcjav induced mild leaf curl symptoms in nicotiana benthamiana plants; dnabeta02 encoded the betac1 gene and produced severe leaf curl symptoms when co-inoculated with tolcjav in n. benthamiana. however, dnabeta02mbetac1, which contains a frame shift mutation, did not induce severe symptoms in n. benthamiana when co-inocul ... | 2007 | 17385070 |
| characterization of signals that dictate nuclear/nucleolar and cytoplasmic shuttling of the capsid protein of tomato leaf curl java virus associated with dna beta satellite. | transport of the viral genome into the nucleus is an obligatory step in the replication cycle of geminiviruses. capsid proteins (cps) of geminiviruses are multifunctional proteins thought to be involved in this process. the cp of monopartite geminiviruses is absolutely essential for virus movement. to more precisely examine the role of cp, we have constructed a series of single and double deletions into the coding sequence of tomato leaf curl java virus (tolcjav) cp and examined sub-cellular loc ... | 2009 | 19409945 |
| tomato leaf curl java virus v2 protein is a determinant of virulence, hypersensitive response and suppression of posttranscriptional gene silencing. | we previously identified the tomato leaf curl java virus-a (tolcjv-a[id]) from southeast asia as a new member of the emerging group of monopartite begomoviruses that require a betasatellite component for symptom induction. in this study, the role of v2 in viral pathogenesis and posttranscriptional gene silencing (ptgs) was studied. our results showed v2 of tolcjv-a[id] elicits a reaction resembling the hypersensitive response (hr) associated with the induction of necrosis and a systemic burst of ... | 2010 | 19896687 |
| subcellular localization of v2 protein of tomato leaf curl java virus by using green fluorescent protein and yeast hybrid system. | tomato leaf curl java virus-a (tolcjv-a[id]) from southeast asia is a new member of the emerging group of monopartite begomoviruses that require a betasatellite component for symptom induction. previously, we have elucidated the role of v1 orf encoded by tolcjv-a[id] in cell-to-cell movement. in this study, the role of v2 (precp) in localization was determined. subcellular localization of tolcjv-a[id] v2 in plant tissues showed that this protein is co-localized to the cell cytoplasm, perinuclear ... | 2011 | 20549267 |
| the natural occurrence of two distinct begomoviruses associated with dnabeta and a recombinant dna in a tomato plant from indonesia. | abstract two begomoviruses (java virus-1 and java virus-2), two satellite dnas (dnabeta01 and dnabeta02), and a recombinant dna (recdna) were cloned from a single tomato plant from indonesia with leaf curl symptoms, and the role of these satellite dnas in the etiology of begomovirus disease was investigated. the genome organizations of the two viruses were similar to those of other old world monopartite begomoviruses. comparison of the sequences with other begomoviruses revealed that java virus- ... | 2006 | 18944312 |
| a begomovirus associated with ageratum yellow vein disease in indonesia: evidence for natural recombination between tomato leaf curl java virus and ageratum yellow vein virus-[java]. | a begomovirus (2747 nucleotides) and a satellite dna beta component (1360 nucleotides) have been isolated from ageratum conyzoides l. plants with yellow vein symptoms growing in java, indonesia. the begomovirus is most closely related to tomato leaf curl java virus (tolcjv) (91 and 98% in the total nucleotide and coat protein amino acid sequences, respectively), although the products of orfs c1 and c4 are more closely related to those of ageratum yellow vein virus-[java] (91 and 95% identity, re ... | 2007 | 17294343 |