| rapid detection of piper yellow mottle virus and cucumber mosaic virus infecting black pepper (piper nigrum) by loop-mediated isothermal amplification (lamp). | the loop-mediated isothermal amplification (lamp) assay for piper yellow mottle virus and the reverse transcription (rt) lamp assay for cucumber mosaic virus each consisted of a set of five primers designed against the conserved sequences in the viral genome. both rna and dna isolated from black pepper were used as a template for the assay. the results were assessed visually by checking turbidity, green fluorescence and pellet formation in the reaction tube and also by gel electrophoresis. the a ... | 2013 | 23791964 |
| influence of temperature on symptom expression, detection of host factors in virus infected piper nigrum l. | expression of symptoms in black pepper plants (piper nigrum) infected with piper yellow mottle virus (pymov) vary depending on the season, being high during summer months. here, we explored the influence of temperature on symptom expression in pymov infected p. nigrum. our controlled environment study revealed increase in virus titer, total proteins, iaa and reducing sugars when exposed to temperature stress. there was change in the 2-d separated protein before and after exposure. the 2-d proteo ... | 2016 | 27319055 |
| sequence diversity among badnavirus isolates infecting black pepper and related species in india. | the badnavirus, piper yellow mottle virus (pymov) is known to infect black pepper (piper nigrum), betelvine (p. betle) and indian long pepper (p. longum) in india and other parts of the world. occurrence of pymov or other badnaviruses in other species of piper and its variability is not reported so far. we have analysed sequence variability in the conserved putative reverse transcriptase (rt)/ribonuclease h (rnase h) coding region of the virus using specific badnavirus primers from 13 virus isol ... | 2014 | 25674613 |
| complete genome sequencing of piper yellow mottle virus infecting black pepper, betelvine, and indian long pepper. | the complete genome of the piper yellow mottle virus (pymov), a badnavirus belonging to the family caulimoviridae, was sequenced from three naturally infected hosts namely, black pepper, betelvine, and indian long pepper. the genome length of the three virus strains (one from each of the three host species) varied from 7,559 to 7,584 nucleotides, and all the three strains possessed four open reading frames (orfs) i to iv that potentially encode proteins of 15.67, 17.08, 218.6, and 17.22 kda, res ... | 2015 | 25331343 |
| the complete genome sequence of piper yellow mottle virus (pymov). | this study reports the first complete genome sequence of piper yellow mottle virus (pymov, kc808712) identified in black pepper. the genome is 7,622 nucleotides long, possessing four open reading frames (orfs). orf1, orf2 and orf4 of pymov are reported as hypothetical proteins of unknown function with a predicted molecular mass of 15.7, 17.1 and 17.9 kda, respectively. orf3 of pymov encodes a polyprotein of 218.6 kda and consists of a viral movement protein (mp), trimeric dutpase, zinc finger, r ... | 2014 | 24005374 |