| preferential pcr amplification of parasitic protistan small subunit rdna from metazoan tissues. | a "universal non-metazoan" polymerase chain reaction (unonmet-pcr) that selectively amplifies a segment of nonmetazoan small subunit (ssu) rdna gene was validated. the primers used were: 18s-euk581-f (5'-gtgccagcagccgcg-3') and 18s-euk1134-r (5'-tttaagtttcagccttgcg-3') with specificity provided by the 19-base reverse primer. its target site is highly conserved across the archaea, bacteria, and eukaryotes (including fungi), but not most metazoa (except porifera, ctenophora, and myxozoa) which hav ... | 2009 | 15218702 |