| cdna, genomic sequence cloning, and overexpression of eif1 from the giant panda (ailuropoda melanoleuca) and the black bear (ursus thibetanus mupinensis). | eukaryotic initiation factor (eif) eif1 is a universally conserved translation factor that is involved in translation initiation site selection. the cdna and the genomic sequences of eif1 were cloned successfully from the giant panda (ailuropoda melanoleuca) and the black bear (ursus thibetanus mupinensis) using reverse transcription polymerase chain reaction (rt-pcr) technology and touchdown-polymerase chain reaction, respectively. the cdnas of the eif1 cloned from the giant panda and the black ... | 2010 | 20589574 |
| a complete mitochondrial genome sequence of asian black bear sichuan subspecies (ursus thibetanus mupinensis). | we obtained the complete mitochondrial genome of u.thibetanus mupinensis by dna sequencing based on the pcr fragments of 18 primers we designed. the results indicate that the mtdna is 16,868 bp in size, encodes 13 protein genes, 22 trna genes, and 2 rrna genes, with an overall h-strand base composition of 31.2% a, 25.4% c, 15.5% g and 27.9% t. the sequence of the control region (cr) located between trna-pro and trna-phe is 1422 bp in size, consists of 8.43% of the whole genome, gc content is 51. ... | 2006 | 17205108 |
| comparative analysis and molecular characterization of a gene banf1 encoded a dna-binding protein during mitosis from the giant panda and black bear. | barrier to autointegration factor 1 (banf1) is a dna-binding protein found in the nucleus and cytoplasm of eukaryotic cells that functions to establish nuclear architecture during mitosis. the cdna and the genomic sequence of banf1 were cloned from the giant panda (ailuropoda melanoleuca) and black bear (ursus thibetanus mupinensis) using rt-pcr technology and touchdown-pcr, respectively. the cdna of the banf1 cloned from giant panda and black bear is 297 bp in size, containing an open reading f ... | 2014 | 25009988 |
| nucleotide sequences of an important functional gene hnrnpa2/b1 from ailuropoda melanoleuca and ursus thibetanus mupinensis and its potential value in phylogenetic study. | the cdna fragments of hnrnpa2/b1 were cloned from the giant panda and black bear using rt-pcr method, which were, respectively, 1029bp and 1026bp in length encoding 343 and 341 amino acids. analysis indicated the cdna cloned from the giant panda encoded variant b1 while the cdna cloned from black bear encoded variant a2. analyzing the hnrnpa2b1 peptide of the giant panda and black bear, 76 glycine residues and 86 glycine residues were, respectively, found, and moreover, most glycine are concentr ... | 2014 | 24588753 |