| vibrio agarivorans sp. nov., a novel agarolytic marine bacterium. | it is proposed that the new vibrio species vibrio agarivorans accommodates two agarolytic, halophilic, fermentative bacterial strains isolated from mediterranean sea water. the cells were gram-negative, oxidase-positive, polarly flagellated bacilli that fermented glucose without gas production and that produced no decarboxylases. they used a wide range of compounds as sole carbon and energy sources. the dna g+c content was 44.8 mol%. phylogenetic analysis based on complete 16s and 23s rdna seque ... | 2001 | 11760944 |
| pseudoalteromonas agarivorans sp. nov., a novel marine agarolytic bacterium. | the phenotypic, genomic and phylogenetic characteristics of four aerobic, gram-negative, non-fermentative, motile, non-pigmented, agarolytic pseudoalteromonas-like bacteria, isolated from marine environments, have been investigated. these bacteria share dna-dna similarities above 86%. comparative 16s rdna sequence analysis of strain kmm 255t revealed its membership of the genus pseudoalteromonas; it shares 99.9% sequence similarity with pseudoalteromonas distincta, pseudoalteromonas elyakovii, p ... | 2003 | 12656163 |
| agarivorans albus gen. nov., sp. nov., a gamma-proteobacterium isolated from marine animals. | six bacterial strains were isolated from healthy marine organisms that were collected from the coast of the kanto area in japan. phylogenetic analysis based on 16s rdna sequence similarity showed that the six isolates formed a separate cluster in the gamma-proteobacteria and were related to the genera alteromonas and glaciecola (<91.6 % similarity). the isolates were related closely to each other (dna-dna reassociation values of 74-93 %). the isolates had a polar flagellum and were gram-negative ... | 2004 | 15143010 |
| high-level expression of a neoagarobiose-producing beta-agarase gene from agarivorans sp. jamb-a11 in bacillus subtilis and enzymic properties of the recombinant enzyme. | the structural gene for a neoagarobiose-producing beta-agarase of an agarivorans isolate was expressed in bacillus subtilis. high-level production of the recombinant enzyme was achieved corresponding to a level of 1.9 x 10(4) units/l of the culture broth. the efficiency of the production is thus 30-fold greater than that of the original strain. the recombinant enzyme (ragaa11) had a molecular mass of 105 kda and a specific activity of 371 units/mg. the optimal ph and temperature of the enzyme we ... | 2005 | 15307821 |
| salegentibacter agarivorans sp. nov., a novel marine bacterium of the family flavobacteriaceae isolated from the sponge artemisina sp. | a sponge-associated strain, kmm 7019t, was investigated in a polyphasic taxonomic study. the bacterium was strictly aerobic, heterotrophic, gram-negative, yellow-pigmented, motile by gliding and oxidase-, catalase-, beta-galactosidase- and alkaline phosphatase-positive. a phylogenetic analysis based on 16s rrna gene sequences revealed that strain kmm 7019t is closely related to members of the genus salegentibacter, namely salegentibacter holothuriorum, salegentibacter mishustinae and salegentiba ... | 2006 | 16585710 |
| thalassomonas agarivorans sp. nov., a marine agarolytic bacterium isolated from shallow coastal water of an-ping harbour, taiwan, and emended description of the genus thalassomonas. | a marine agarolytic bacterium, designated strain tma1(t), was isolated from a seawater sample collected in a shallow-water region of an-ping harbour, taiwan. it was non-fermentative and gram-negative. cells grown in broth cultures were straight or curved rods, non-motile and non-flagellated. the isolate required nacl for growth and exhibited optimal growth at 25 degrees c and 3 % nacl. it grew aerobically and was incapable of anaerobic growth by fermenting glucose or other carbohydrates. predomi ... | 2006 | 16738099 |
| cloning, expression, and characterization of a glycoside hydrolase family 50 beta-agarase from a marine agarivorans isolate. | the gene for a thermostable beta-agarase from agarivorans sp. ja-1 was cloned and sequenced. it comprised an open reading frame of 2,988 base pairs, which encode a protein of 109,450 daltons consisting of 995 amino acid residues. a comparison of the entire sequence showed that the enzyme has 98.8% sequence similarities to beta-agarase from vibrio sp. jt1070, indicating that it belongs to the family glycoside hydrolase (gh)-50. the gene corresponding to a mature protein of 976 amino acids was ins ... | 2006 | 17028783 |
| metalloid reducing bacteria isolated from deep ocean hydrothermal vents of the juan de fuca ridge, pseudoalteromonas telluritireducens sp. nov. and pseudoalteromonas spiralis sp. nov. | five strains of gram-negative, rod, curved rod and spiral-shaped bacteria were isolated from the vicinity of deep ocean hydrothermal vents along the main endeavour segment of the juan de fuca ridge in the pacific ocean. all strains showed remarkable resistance to high levels of toxic metalloid oxyanions, and were capable of reducing the oxyanions tellurite and selenite to their less toxic elemental forms. phylogenetic analysis of four strains identified these isolates as close relatives of the g ... | 2006 | 17066332 |
| purification and characterization of a novel beta-agarase, agaa34, from agarivorans albus ykw-34. | an extracellular beta-agarase (agaa34) was purified from a newly isolated marine bacterium, agarivorans albus ykw-34 from the gut of a turban shell. agaa34 was purified to homogeneity by ion exchange and gel filtration chromatographies with a recovery of 30% and a fold of ten. agaa34 was composed of a single polypeptide chain with the molecular mass of 50 kda. n-terminal amino acid sequencing revealed a sequence of aslvtsfeea, which exhibited a high similarity (90%) with those of agarases from g ... | 2008 | 18071641 |
| over-production of a glycoside hydrolase family 50 beta-agarase from agarivorans sp. ja-1 in bacillus subtilis and the whitening effect of its product. | the gene for beta-agarase of an agarivorans sp. ja-1 was expressed in bacillus subtilis strain db104 for efficient and economical mass-production of the enzyme. we isolated 360 mg protein with a specific activity of 201 u/mg from the culture broth. the efficiency of production was approximately 130-fold higher than that in e. coli. the enzyme produced neoagarohexaose, neoagarotetraose and neoagarobiose from agar. neoagarooligosaccharides produced by the enzyme had a whitening effect and inhibite ... | 2008 | 18189120 |
| simiduia agarivorans gen. nov., sp. nov., a marine, agarolytic bacterium isolated from shallow coastal water from keelung, taiwan. | a gram-negative, heterotrophic, agarolytic, marine bacterium, designated strain sa1t, was isolated from a seawater sample collected in the shallow coastal region of keelung, taiwan. cells were straight to slightly curved rods. nearly all of the cells were non-motile and non-flagellated during the exponential phase of growth in broth cultures; a few cells (<1 %) were motile and were considered to have monotrichous flagella. the isolate required nacl for growth and grew optimally at 30-35 degrees ... | 2008 | 18398190 |
| tamlana agarivorans sp. nov., isolated from seawater off jeju island in korea. | a gram-negative, non-motile, rod-shaped, agarolytic bacterial strain, designated jw-26t, was isolated from seawater off jeju island in korea, and was subjected to a polyphasic taxonomic investigation. strain jw-26t grew optimally at ph 7.0-8.0, at 30 degrees c and in the presence of approximately 2 % (w/v) nacl. phylogenetic analyses based on 16s rrna gene sequences revealed that strain jw-26t joins tamlana crocina hst1-43t, with which it exhibited a 16s rrna gene sequence similarity value of 95 ... | 2008 | 18676475 |
| a new high-alkaline alginate lyase from a deep-sea bacterium agarivorans sp. | a high-alkaline, salt-activated alginate lyase is produced by agarivorans sp. jam-a1m from a deep-sea sediment off cape nomamisaki on kyushu island, japan. purified to homogeneity, as judged by sds-page, the enzyme (a1m) had a molecular mass of approximately 31 kda. the optimal ph was around 10 in glycine-naoh buffer, and the activity was increased to 1.8 times by adding 0.2 m nacl. however, when the optimal ph in the presence of 0.2 m nacl was shifted to ph 9.0, the activity was more than 10 ti ... | 2009 | 19002649 |
| production and purification of agarase from a marine agarolytic bacterium agarivorans sp. hz105. | isolation and characterization of an agarase-producing bacterium agarivorans sp. hz105. | 2009 | 19054239 |
| gene cloning, expression, and characterization of a beta-agarase, agab34,from agarivorans albus ykw-34. | a beta-agarase gene, agab34, was functionally cloned from the genomic dna of a marine bacterium, agarivorans albus ykw-34. the open reading frame of agab34 consisted of 1,362 bp encoding 453 amino acids. the deduced amino acid sequence, consisting of a typical n-terminal signal peptide followed by a catalytic domain of glycoside hydrolase family 16 (gh-16) and a carbohydrate-binding module (cbm), showed 37-86% identity to those of agarases belonging to family gh-16. the recombinant enzyme (ragab ... | 2009 | 19349750 |
| a novel beta-agarase with high ph stability from marine agarivorans sp. lq48. | a novel endo-type beta-agarase gene, agaa, was cloned from a newly isolated marine bacterium, agarivorans sp. lq48. it encodes a protein of 457 amino acids with a calculated molecular mass of 51.2 kda. the deduced protein contains a typical n-terminal signal peptide of 25 amino acid residues, followed by a catalytic module, which is homologous to that of glycoside hydrolase family 16. a sequence similar to a carbohydrate-binding module is found in the c-terminal region of the enzyme. the overall ... | 2010 | 19484308 |
| psychromonas agarivorans sp. nov., a novel agarolytic bacterium. | two heterotrophic, agarolytic bacteria were isolated from marine environments. a 16s rrna gene sequence analysis showed the isolates (designated strains j42-3a(t) and 04oz-as15-7a) to be positioned in a separate lineage within the genus psychromonas. members of the genus psychromonas are recognized as being psychrophilic or psychrotolerant, whereas none of the currently established psychromonas species is known to be agarolytic. the dna g+c contents of the isolates were about 42 mol% and the pre ... | 2009 | 19502298 |
| aliagarivorans marinus gen. nov., sp. nov. and aliagarivorans taiwanensis sp. nov., facultatively anaerobic marine bacteria capable of agar degradation. | two agarolytic strains of gram-negative, heterotrophic, facultatively anaerobic, marine bacteria, designated aam1t and aat1t, were isolated from seawater samples collected in the shallow coastal region of an-ping harbour, tainan, taiwan. cells grown in broth cultures were straight rods that were motile by means of a single polar flagellum. the two isolates required nacl for growth and grew optimally at about 25-30 degrees c, in 2-4% nacl and at ph 8. they grew aerobically and could achieve anaer ... | 2009 | 19567569 |
| vibrio hangzhouensis sp. nov., isolated from sediment of the east china sea. | strain cn83t, a gram-negative, aerobic, rod-shaped bacterium, was isolated from sediment of the east china sea. the isolate was catalase- and oxidase-positive and cells were motile by means of polar flagella. the dna g+c content was 44.9 mol%. the major fatty acids were c16:1omega7c and/or iso-c15:0 2-oh, c16:0, c18:1omega7c, c14:0 and c12:0. 16s rrna gene sequence analysis showed that strain cn83t belonged to the genus vibrio and had the highest sequence similarity to vibrio agarivorans (98.4%) ... | 2009 | 19605706 |
| umboniibacter marinipuniceus gen. nov., sp. nov., a marine gammaproteobacterium isolated from the mollusc umbonium costatum from the sea of japan. | two bacterial strains, kmm 3891(t) and kmm 3892, were isolated from internal tissues of the marine mollusc umbonium costatum collected from the sea of japan. the novel isolates were gram-negative, aerobic, faint pink-reddish-pigmented, rod-shaped, non-motile, stenohaline and psychrotolerant bacteria that were unable to degrade most tested complex polysaccharides. polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. fatty acid analysis revealed c(17 ... | 2010 | 19654350 |
| [isolation and characterization of a marine agarase]. | this study was carried out to isolate and characterize an agarase from a marine bacterium agarivorans albus qm38. | 2009 | 19873753 |
| cloning and sequencing of alginate lyase genes from deep-sea strains of vibrio and agarivorans and characterization of a new vibrio enzyme. | four alginate lyase genes were cloned and sequenced from the genomic dnas of deep-sea bacteria, namely members of vibrio and agarivorans. three of them were from vibrio sp. jam-a9m, which encoded alginate lyases, a9mt, a9mc, and a9ml. a9mt was composed of 286 amino acids and 57% homologous to alxm of photobacterium sp. a9mc (221 amino acids) and a9ml (522 amino acids) had the highest degree of similarity to two individual alginate lyases of vibrio splendidus with 74% and 84% identity, respective ... | 2010 | 19941025 |
| agarivorans gilvus sp. nov. isolated from seaweed. | a novel agarase-producing, non-endospore-forming marine bacterium, wh0801(t), was isolated from a fresh seaweed sample collected from the coast of weihai, china. preliminary characterization based on 16s rrna gene sequence analysis showed that wh0801(t) shared 96.1 % similarity with agarivorans albus mkt 106(t), the type species of the genus agarivorans. a polyphasic taxonomic study was conducted and confirmed the phylogenetic affiliation of strain wh0801(t) to the genus agarivorans. isolate wh ... | 2011 | 20363930 |
| structure of an acidic o-specific polysaccharide of the marine bacterium pseudoalteromonas agarivorans kmm 232 (r-form). | an acidic o-specific polysaccharide containing l-rhamnose, 2-acetamido-2-deoxy-d-galactose, 2,6-dideoxy-2-(n-acetyl-l-threonine)amino-d-galactose, and 2-acetamido-2-deoxy-d-mannuronic acid was obtained by mild acid degradation of the lipopolysaccharide of the marine bacterium pseudoalteromonas agarivorans kmm 232 (r-form) followed by gel-permeation chromatography. the polysaccharide was subjected to smith degradation to give a modified polysaccharide with trisaccharide repeating unit containing ... | 2010 | 20632942 |
| culture-independent study of the diversity of microbial populations in brines during fermentation of naturally-fermented aloreña green table olives. | aloreña table olives are naturally fermented traditional green olives with a denomination of protection (dop). the present study focused on aloreña table olives manufactured by small and medium enterprises (smes) from valle del guadalhorce (southern spain) under three different conditions (cold storage, and ambient temperature fermentations in small vats and in large fermentation tanks). the microbial load of brines during fermentation was studied by plate counting, and the microbial diversity w ... | 2010 | 21122933 |
| thalassomonas agariperforans sp. nov., an agarolytic bacterium isolated from marine sand. | a gram-negative, motile, agarolytic bacterium, designated m-m1(t), was isolated from marine sand obtained from geoje island, south sea, korea, and its taxonomic position was investigated using a polyphasic taxonomic approach. strain m-m1(t) grew optimally at ph 7.0-8.0, at 30 °c and in the presence of 2 % (w/v) nacl. it did not grow in the presence of > 7 % (w/v) nacl. phylogenetic analysis based on 16s rrna gene sequences showed that strain m-m1(t) fell within the clade comprising members of th ... | 2010 | 21131503 |
| catenovulum agarivorans gen. nov. sp. nov., a peritrichously flagellated, catenated, agar-hydrolyzing {gamma}-proteobacterium isolated from the yellow sea. | a novel gram-negative, strictly aerobic, agar-hydrolyzing bacterium designated ym01(t) was isolated from sea water samples collected from the yellow sea (coastal region of qingdao, p.r. china). cells were rod-shaped, peritrichously flagellated and formed long chains by end-to-end. this isolate absolutely required na(+) ions but not sea water for growth, and grew optimally at about 28° c, 2 % nacl and ph 8-9. this isolate could not be cultured in marine broth 2216 without agar. ym01(t) was able t ... | 2011 | 21257691 |
| bioactivity, chemical profiling, and 16s rrna-based phylogeny of pseudoalteromonas strains collected on a global research cruise. | one hundred one antibacterial pseudoalteromonas strains that inhibited growth of a vibrio anguillarum test strain were collected on a global research cruise (galathea 3), and 51 of the strains repeatedly demonstrated antibacterial activity. here, we profile secondary metabolites of these strains to determine if particular compounds serve as strain or species markers and to determine if the secondary metabolite profile of one strain represents the bioactivity of the entire species. 16s rrna gene ... | 2011 | 21305330 |
| simiduia areninigrae sp. nov., a novel agarolytic bacterium isolated from sea sand. | during a study intended to screen for agar degrading bacteria, strain m2-5(t), was isolated from black sand off the shore of jeju island, republic of korea. strain m2-5(t) exhibited agarase activity and had a ╬▓-agarase gene with 62% amino acid sequence identity to the ╬▓-agarase gene of microbulbifer thermotolerans jamb-a94. the isolate was closely related to the genus simiduia but was clearly discernible from the reported simiduia species, according to polyphasic analysis. strain m2-5(t) was g ... | 2011 | 21669928 |