| complete sequence of a picorna-like virus of the genus iflavirus replicating in the mite varroa destructor. | aggregations of 27 nm virus-like particles were observed in electron microscopy images of sectioned varroa destructor mite tissue. the scattered occurrence of individual particles and accumulation of the virions in lattices in the cytoplasm gave an apparent indication that the virus replicates in the mite. sequence analysis of the rna of the purified virus revealed a genome organization with high similarity to that of members of the genus iflavirus. phylogenetic analysis of the polymerase showed ... | 2004 | 15557248 |
| kakugo virus from brains of aggressive worker honeybees. | | 2005 | 16387192 |
| deformed wing virus is not related to honey bees' aggressiveness. | guards of cyprian honey bee colonies, apis mellifera cypria, display a great defensive behaviour against hornets' attacks. the deformed wing virus (dwv) and the kakugo virus (kv) genomes are very similar, but unlike kv, the presence of dwv is not related to honey bees' aggressiveness. this discrepancy is further discussed. | 2006 | 16942620 |
| prevalence and phylogeny of kakugo virus, a novel insect picorna-like virus that infects the honeybee (apis mellifera l.), under various colony conditions. | we previously identified a novel insect picorna-like virus, termed kakugo virus (kv), from the brains of aggressive worker honeybees that had counterattacked a giant hornet. to survey the prevalence of kv in worker populations engaged in various labors, we quantified kv genomic rna. kv was detected specifically from aggressive workers in some colonies, while it was also detected from other worker populations in other colonies where the amount of kv detected in the workers was relatively high, su ... | 2006 | 16971448 |
| phylogenetic analysis of deformed wing virus genotypes from diverse geographic origins indicates recent global distribution of the virus. | honeybees originating from 10 different countries (austria, poland, germany, hungary, slovenia, nepal, sri lanka, the united arab emirates, canada, and new zealand) located on four continents were analyzed for the presence of deformed wing virus (dwv) nucleic acid by reverse transcription-pcr. two target regions within the dwv genome were selected for pcr amplification and subsequent sequencing, i.e., a region within the putative vp2 and vp4 structural-protein genes and a region within the rna h ... | 2007 | 17435003 |
| the use of rna-dependent rna polymerase for the taxonomic assignment of picorna-like viruses (order picornavirales) infecting apis mellifera l. populations. | single-stranded rna viruses, infectious to the european honeybee, apis mellifera l. are known to reside at low levels in colonies, with typically no apparent signs of infection observed in the honeybees. reverse transcription-pcr (rt-pcr) of regions of the rna-dependent rna polymerase (rdrp) is often used to diagnose their presence in apiaries and also to classify the type of virus detected. | 2008 | 18211671 |
| detection of a honeybee iflavirus with intermediate characteristics between kakugo virus and deformed wing virus. | iflavirus rna was detected in honeybee colonies displaying unduly aggressive behavior and with no evidence of morphological alterations. sequence analysis of the rna-dependent rna polymerase (rdrp) revealed that the iflavirus strain was more similar (> 99% aa) to deformed wing virus (dwv), that has been associated with morphological alterations in bees, rather than to the newly-described kakugo virus (kv) (about 95% aa), that has been associated with increased aggressiveness. therefore, the ifla ... | 2008 | 19123297 |
| distribution of kakugo virus and its effects on the gene expression profile in the brain of the worker honeybee apis mellifera l. | we previously identified a novel insect picorna-like virus, termed kakugo virus (kv), obtained from the brains of aggressive honeybee worker bees that had counterattacked giant hornets. here we examined the tissue distribution of kv and alterations of gene expression profiles in the brains of kv-infected worker bees to analyze possible effects of kv infection on honeybee neural and physiological states. by use of in situ hybridization, kv was broadly detected in the brains of the naturally kv-in ... | 2009 | 19726502 |
| iridovirus and microsporidian linked to honey bee colony decline. | in 2010 colony collapse disorder (ccd), again devastated honey bee colonies in the usa, indicating that the problem is neither diminishing nor has it been resolved. many ccd investigations, using sensitive genome-based methods, have found small rna bee viruses and the microsporidia, nosema apis and n. ceranae in healthy and collapsing colonies alike with no single pathogen firmly linked to honey bee losses. | 2010 | 20949138 |
| [rt-pcr detection of deformed wing virus in the honey bee apis mellifera l. in the moscow region]. | deformed wing virus (dwv) was first detected in the honey bee apis mellifera by reverse transcriptase-polymerase chain reaction (rt-pct) in the moscow region. molecular phylogenetic analysis of the detected nucleotide sequence of the virus fragment vp2-vp1 of dwv demonstrated that the russian virus sequence is united in the common cluster with all earlier revealed nucleotide sequences of dwv in the genbank worldwide, which confirms the previous conclusions that this virus has recently distribute ... | 2010 | 21260995 |
| virus infections of honeybees apis mellifera. | the health and vigour of honeybee colonies are threatened by numerous parasites (such as varroa destructor and nosema spp.) and pathogens, including viruses, bacteria, protozoa. among honeybee pathogens, viruses are one of the major threats to the health and well-being of honeybees and cause serious concern for researchers and beekeepers. to tone down the threats posed by these invasive organisms, a better understanding of bee viral infections will be of crucial importance in developing effectiv ... | 2015 | 27800411 |
| molecular characterization and phylogenetic analysis of deformed wing viruses isolated from south korea. | deformed wing virus (dwv) is one of the most common viral infection in honeybees. phylogenetic trees were constructed for 16 partial nucleotide sequences of the structural polyprotein region and the rna helicase region of south korean dwvs. the sequences were compared with 10 previously reported dwv sequences from different countries and the sequences of two closely related viruses, kakugo virus (kgv) and varroa destructor virus-1 (vdv-1). the phylogeny based on these two regions, the korean dwv ... | 2013 | 24035266 |