| effect of extracts of orthosiphon stamineus benth, hieracium pilosella l., sambucus nigra l. and arctostaphylos uva-ursi (l.) spreng. in rats. | aqueous extracts of sambucus nigra and arctostaphylos uva-ursi and hydroalcohol extracts of orthosiphon stamineus and hieracium pilosella were tested for their diuretic activities in rats; pharmacological evaluation revealed that they led to an increase in urine flow. urinary sodium excretion in rats was increased with o. stamineus and s. nigra. | 1999 | 10353162 |
| four highly oxygenated isopimarane-type diterpenes of orthosiphon stamineus. | four highly oxygenated isopimarane-type diterpenes, named orthosiphols o, p and q and nororthosiphonolide a, have been isolated from the aerial parts of orthosiphon stamineus from myanmar, together with three known diterpenes, orthosiphols d and e and orthosiphonone a. their structures were determined on the basis of extensive spectral analysis. all the isolated compounds displayed mild antiproliferative activities against highly liver metastatic colon 26-l5 carcinoma and human ht-1080 fibrosarc ... | 2002 | 11914974 |
| hplc and anti-inflammatory studies of the flavonoid rich chloroform extract fraction of orthosiphon stamineus leaves. | the aim of the present study was to verify the anti-inflammatory activity of orthosiphon stamineus leaf extracts and to identify the active compound(s) contributing to its anti-inflammatory activity using a developed hplc method. active chloroform extract of o. stamineus was fractionated into three fractions using a dry flash column chromatography method. these three fractions were investigated for anti-peritoneal capillary permeability, in vitro nitric oxide scavenging activity, anti-inflammato ... | 2010 | 20657453 |
| on the effects of a plant extract of orthosiphon stamineus on sebum-related skin imperfections. | overproduction of sebum is very common and results in an undesirable oily, shiny complexion with enlarged pores. sebum secretion is basically under the control of 5-α reductase, and more particularly under that of type 1 isozyme. but it is also highly sensitive to environmental factors such as temperature, humidity and food. moreover, in asia, the edicts of a flawless facial skin turn oily skin into a major concern for asian women. we identified orthosiphon stamineus leaf extract as an interesti ... | 2011 | 20807263 |
| toxicity evaluation of a standardised 50% ethanol extract of orthosiphon stamineus. | the present investigation was carried out to evaluate the safety of standardised 50% ethanol extract of orthosiphon stamineus plant by determining its potential toxicity after acute and subchronic administration in rats. | 2010 | 20937371 |
| in vitro modulatory effects of andrographis paniculata, centella asiatica and orthosiphon stamineus on cytochrome p450 2c19 (cyp2c19). | ethno pharmacological relevance: andrographis paniculata (ap), centella asiatica (ca) and orthosiphon stamineus (os) are three popular herbs traditionally used worldwide. ap is known for the treatment of infections and diabetes and ca is good for wound healing and healthy skin while os is usually consumed as tea to treat kidney and urinary disorders. interaction of these herbs with human cytochrome p450 2c19 (cyp2c19), a major hepatic cyp isoform involved in metabolism of many clinical drugs has ... | 2010 | 21093571 |
| effects of andrographis paniculata and orthosiphon stamineus extracts on the glucuronidation of 4-methylumbelliferone in human ugt isoforms. | the effects of andrographis paniculata and orthosiphon stamineus extracts on the in vitro glucuronidation of 4-methylumbelliferone (4mu) by recombinant human ugts, ugt1a1, ugt1a3, ugt1a6, ugt1a7, ugt1a8, ugt1a10, ugt2b7 and ugt2b15 were determined. the potential inhibitory effects of both of the extracts on the activity of each of the ugt isoforms were investigated using 4mu as the substrate. incubations contained udp-glucuronic acid (udpga) as the cofactor, mgcl(2), cell lysate of respective is ... | 2010 | 20657500 |
| orthosiphon stamineus leaf extract protects against ethanol-induced gastropathy in rats. | orthosiphon stamineus benth., which is used as a gastroprotective herbal remedy in malaysia, was assessed for its anti-ulcerogenic activity against ethanol-induced ulcers in rats. fifty percent methanol was used to extract the oven-dried o. stamineus leaves. the extract was then lyophilized with a rotary evaporator and freeze-dried. oral administration of o. stamineus methanolic extract (osme) (125, 250, 500, and 1,000 mg/kg) was found to significantly decrease the ulcer index (p < .01, p < .001 ... | 2009 | 19857074 |
| diuretic properties of orthosiphon stamineus benth. | orthosiphon stamineus has been used in traditional medicine for centuries especially to treat diseases of the urinary system. | 2009 | 19375494 |
| evaluation of the anti-pyretic potential of orthosiphon stamineus benth standardized extract. | the anti-pyretic activity of a standardized methanol/water (50/50) extract of orthosiphon stamineus benth. (seos) was investigated for its effect on normal body temperature and yeast-induced pyrexia in sprague dawley (sd) rats. the seos showed no effect on normal body temperature. doses of 500 and 1000 mg/kg body weight of seos significantly reduced the yeast-induced elevation in body temperature. this effect persisted up to 4 h following the administration of the extract. the anti-pyretic effec ... | 2009 | 19127348 |
| determination of betulinic acid from orthosiphon stamineus leaf extract in rat plasma. | a simple high-performance liquid chromatography (hplc) method to determine the content of betulinic acid (ba) in rat plasma collected at different times (0-8 h) after oral administration of orthosiphon stamineus leaf extract was developed. the features of the assay include protein precipitation using acetonitrile and isocratic elution using reverse phase c-18 column with ultraviolet (uv) detection. the recovery of ba from plasma varied from 98.4 to 102.5%. the r.s.d of intra- and inter-day preci ... | 2008 | 19069237 |
| studies on diuretic and hypouricemic effects of orthosiphon stamineus methanol extracts in rats. | orthosiphon stamineus (labiatae) is a traditional folk medicine widely used in southeast asia for the treatment of several kidney disorders, gout and as a diuretic. this study was conducted to examine the diuretic and hypouricemic effects of orthosiphon stamineus leaf extracts. | 2008 | 18602231 |
| toxicity study of orthosiphon stamineus benth (misai kucing) on sprague dawley rats. | orthosiphon stamineus benth (family: lamiaceae) or locally known as misai kucing has been widely used in malaysia for treating kidney problems, gout, and diabetes. this study aims to evaluate the possible toxic effect after following fourteen days oral administration of methanol extract of o. stamineus in female sprague dawley (sd) rats. control groups were treated orally with distilled water (vehicle) while the four test groups were treated up to fourteen days with 0.5 g/kg, 1 g/kg, 3 g/kg and ... | 2008 | 18600199 |
| an investigation of the anti-inflammatory and analgesic effects of orthosiphon stamineus leaf extract. | anti-inflammatory and analgesic activities of a standardized orthosiphon stamineus methanol:water (50:50 vol/vol) leaf extract (seos) were evaluated in animal models. oral administration of seos at doses of 500 and 1,000 mg/kg significantly reduced the hind paw edema in rats at 3 and 5 hours after carrageenan administration (p < .01 and p < .01; p < .01 and p < .05, respectively). seos (1,000 mg/kg, p.o.) also produced significant (p < .05) analgesic activity in both the acetic acid-induced writ ... | 2008 | 18598181 |
| acute toxicity of orthosiphon stamineus benth standardized extract in sprague dawley rats. | the acute toxicity of standardized extract of orthosiphon stamineus was studied in sprague dawley rats. the rats were administered a single dose of 5000 mg/kg body weight (bw) orally on day 0 and observed for 14 days. there were no deaths recorded and the animals did not show signs of toxicity during the experimental period. the effect of the extract on general behavior, bw, food and water intake, relative organ weight per 100 g bw, hematology and clinical biochemistry were measured. all the par ... | 2009 | 17498941 |
| antioxidant and hepatoprotective effects of orthosiphon stamineus benth. standardized extract. | orthosiphon stamineus (os), benth. (lamiaceae) is widely used in malaysia for treatments of various kidney and liver ailments. in the experiment, dpph* radicals scavenging, fe(3+)-induced lipid peroxidation inhibiting activities and trolox equivalent antioxidant capacity (teac) of methanol/water extract of orthosiphon stamineus (seos) were determined. the results indicated that seos exhibited antioxidant, lipid peroxidation inhibition and free radical scavenging activities. the hepatoprotective ... | 2007 | 17265556 |
| effects of orthosiphon stamineus aqueous extract on plasma glucose concentration and lipid profile in normal and streptozotocin-induced diabetic rats. | the objective of this study was to investigate the effects of orthosiphon stamineus benth. aqueous extract on plasma glucose concentration and lipid profile in normal and streptozotocin-induced diabetic rats. the chemical screening of the extract showed phenolic compound and flavonoid content were 13.24+/-0.33 mg/g and 1.73+/-0.14 microg/g, respectively. in oral glucose tolerance test, the extract (0.2-1.0 g/kg) significantly decreased plasma glucose concentration in a dose-dependent manner in b ... | 2007 | 17034971 |
| determination of flavonoids from orthosiphon stamineus in plasma using a simple hplc method with ultraviolet detection. | a simple liquid chromatographic method was developed for the simultaneous determination of flavonoids from orthosiphon stamineus benth, namely sinensitin, eupatorin and 3'-hydroxy-5,6,7,4'-tetramethoxyflavone, in plasma. prior to analysis, the flavonoids and the internal standard (naproxen) were extracted from plasma samples using a 1:1 mixture of ethyl acetate and chloroform. the detection and quantification limits for the three flavonoids were similar being 3 and 5 ng/ml, respectively. the wit ... | 2005 | 15664346 |
| phytochemical and pharmacological studies on orthosiphon stamineus benth. (lamiaceae) hydroalcoholic extracts. | the main components of orthosiphon stamineus benth. leaves and extracts are the pharmacologically active polyphenols: the polymethoxylated flavonoids and the caffeic acid derivatives. two tinctures, having different alcohol concentration, were studied from phytochemical and pharmacological point of view. the main polyphenols were identified and quantitatively determined by hplc. comparison of the retention parameters and uv-vis spectra of standards and those of the separated compounds performed ... | 2003 | 12946538 |
| chemical constituents and biological activities of vietnamese medicinal plants. | every country has its own traditional medicine system originated from ancient times. vietnam, a southeast asian country, has a unique traditional medicine system. several medicinal plants are still used by different ethnic societies of vietnam for treating several diseases due to their easy availability. combretum quadrangulare, panax vietnamensis (vietnamese ginseng), dracaena angustifolia (nam ginseng) and orthosiphon stamineus are among the popular medicinal plants used in vietnamese traditio ... | 2003 | 12570775 |
| five novel highly oxygenated diterpenes of orthosiphon stamineus from myanmar. | five novel highly oxygenated diterpenes, orthosiphols k (1), l (2), m (3), and n (4) and norstaminone a (5), were isolated from the aerial part of orthosiphon stamineus, together with three known diterpenes, orthosiphols a (6) and b (7) and neoorthosiphol a (8). orthosiphol l (2) is an isopimarane-type diterpene with a hydroxyl group at c-12, which supports the biogenesis of staminane-type diterpenes, i.e., migration of a vinylic group from c-13 of isopimarane to c-12. norstaminone a (5) has a s ... | 2001 | 11374950 |
| [adsorbed plant extracts, use of extracts of dried seeds of orthosiphon stamineus benth]. | the work presented is related to the elaboration and the control of spray dried vegetable extracts of orthosiphon stamineus benth. from these studies, it feasibility of the spray dried extracts. the best result concerning a pulverulent structure and the stability under moisture conditions is obtained using aerosil 200. pharmacological studies on rats have shown a diuretic activity under our experimental conditions with alcoholic and water extracts. | 1989 | 2780755 |
| studies on the individual and combined diuretic effects of four vietnamese traditional herbal remedies (zea mays, imperata cylindrica, plantago major and orthosiphon stamineus). | herbal remedies are widely used in vietnam alongside modern drugs. we assessed the diuretic effect of four traditional vietnamese herbal remedies from zea mays, imperata cylindrica, plantago major and orthosiphon stamineus, all claimed to produce an increase of diuresis. no influence was recorded for the 12- and 24-h urine output or on the sodium excretion for any of the drugs when tested under standardized conditions in a placebo controlled double-blind crossover model. the present study indica ... | 1992 | 1434681 |
| nutraceuticals for blood pressure control in patients with high-normal or grade 1 hypertension. | current hypertension management guidelines do not recommend drug treatment in subjects with blood pressure (bp) in the high-normal range due to the risk of side effects of the currently available antihypertensive agents that overcomes the possible benefit. nutraceuticals are free from relevant side effects and could be a valuable strategy for the treatment of these patients. | 2012 | 22994579 |
| antiadhesion as a functional concept for protection against uropathogenic escherichia coli: in vitro studies with traditionally used plants with antiadhesive activity against uropathognic escherichia coli. | investigation of medicinal plant extracts traditionally used against uncomplicated urinary tract infections (uti) and identification of antiadhesive effects under in vitro conditions against binding of uropathogenic escherichia coli (upec) on bladder cell surface. | 2013 | 23211661 |
| phytochemical analysis of andrographis paniculata and orthosiphon stamineus leaf extracts for their antibacterial and antioxidant potential. | leaves of andrographis paniculata and orthosiphon stamineus were extracted with water, ethanol, methanol and chloroform to assess their potential as antibacterial and antioxidant agents. high performance liquid chromatography analysis showed that the methanolic extracts of a. paniculata and o. stamineus leaves gave the highest amounts of andrographolide and rosmarinic acid, respectively. these leaf extracts exhibited antimicrobial and antioxidant activities and, at the highest concentration test ... | 2013 | 24189677 |
| cat's whiskers tea (orthosiphon stamineus) extract inhibits growth of colon tumor in nude mice and angiogenesis in endothelial cells via suppressing vegfr phosphorylation. | cat's whiskers (orthosiphon stamineus) is commonly used as java tea to treat kidney stones including a variety of angiogenesis-dependent diseases such as tumorous edema, rheumatism, diabetic blindness, and obesity. in the present study, antitumor potential of standardized 50% ethanol extract of o. stamineus leaves (eos) was evaluated against colorectal tumor in athymic mice and antiangiogenic efficacy of eos was investigated in human umbilical vein endothelial cells (huvec). eos at 100 mg/kg cau ... | 2011 | 22136553 |
| hepatoprotective effects of orthosiphon stamineus extract on thioacetamide-induced liver cirrhosis in rats. | orthosiphon stamineus as medicinal plant is commonly used in malaysia for treatment of hepatitis and jaundice; in this study, the ethanol extracts were applied to evaluate the hepatoprotective effects in a thioacetamide-induced hepatotoxic model in sprague dawley rats. five groups of adult rats were arranged as follows: group 1 (normal control group), group 2 thioacetamide (taa) as positive control (hepatotoxic group), group 3 silymarin as a well-known standard drug (hepatoprotective group), and ... | 2011 | 21647311 |
| antihyperglycemic effect of orthosiphon stamineus benth leaves extract and its bioassay-guided fractions. | preliminary investigations were carried out to evaluate the antidiabetic effects of the leaves of o. stamineus extracted serially with solvents of increasing polarity (petroleum ether, chloroform, methanol and water); bioassay-guided purification of plant extracts using the subcutaneous glucose tolerance test (sbgtt) was also carried out. only the chloroform extract, given at 1 g/kg body weight (b.w.), significantly reduced (p < 0.05) the blood glucose level of rats loaded subcutaneously with 15 ... | 2011 | 21544041 |
| in vitro effects of active constituents and extracts of orthosiphon stamineus on the activities of three major human cdna-expressed cytochrome p450 enzymes. | orthosiphon stamineus (os) has been traditionally used to treat diabetes, kidney and urinary disorders, high blood pressure and bone or muscular pain. to assess the possibility of drug-herb interaction via interference of metabolism, effects of four os extracts of different polarity and three active constituents (sinensetin, eupatorin and rosmarinic acid) on major human cdna-expressed cytochrome p450 (cyp) enzymes were investigated. three substrate-probe based high-performance liquid chromatogra ... | 2011 | 21276781 |
| evaluation of the genotoxicity of orthosiphon stamineus aqueous extract. | orthosiphon stamineus, benth, also known as misai kucing in malaysia and java tea in indonesia, is traditionally used in southeastern asia to treat kidney dysfunctions, diabetes, gout and several other illnesses. recent studies of orthosiphon stamineus pharmacological profile have revealed antioxidant properties and other potentially useful biological activities thereby lending some scientific support to its use in folk medicine. so far the genotoxicity of orthosiphon stamineus extracts has not ... | 2010 | 21044879 |
| free radical scavenging, antimicrobial and immunomodulatory activities of orthosiphon stamineus. | orthosiphon stamineus is considered an important traditional folk medicine. in this study ethanol and aqueous extracts of o. stamineus were evaluated in vitro for their antioxidant, antimicrobial as well as for their immunomodulatory properties on human peripheral blood mononuclear cells (pbmcs). the dpph radical scavenging method was used for the determination of antioxidant activity, while the antibacterial efficacy was investigated by both disc diffusion method and minimum inhibitory concentr ... | 2012 | 22569417 |
| complementary alternative medicine use among patients with type 2 diabetes mellitus in the primary care setting: a cross-sectional study in malaysia. | limited study on the use of complementary alternative medicine (cam) among patients with diabetes mellitus (dm), particularly in primary -care settings. this study seeks to understand the prevalence, types, expenditures, attitudes, beliefs, and perceptions of cam use among patients with dm visiting outpatient primary care clinics. | 2013 | 23802882 |
| tongkat ali (eurycoma longifolia): a possible therapeutic candidate against blastocystis sp. | in the local malaysian context, herbal plants such as eurycoma longifolia (tongkat ali), orthosiphon stamineus (misaikucing), ficus deltoidea (mas cotek), zingiber officinale (halia bara) and barringtonia racemosa (putat) are known and widely used for its therapeutic properties. the first part of this study aims to screen for the anti-protozoal activity of these herbal plant extracts against blastocystis sp. isolate subtype (st) 3. herbal extract with the highest efficacy was further fractionize ... | 2015 | 26082155 |
| inhibition of cytosolic phospholipase a2α (cpla2α) by medicinal plants in relation to their phenolic content. | the cytosolic phospholipase a2α(cpla2α) is one of the potential targets for anti-inflammatory drugs, since this enzyme plays a key role in the inflammation processes seen in health disorders, like asthma, allergic reactions, arthritis and neuronal diseases. in this study, cpla2α inhibition by 43 methanol extracts from medicinal plants rich in polyphenols was determined. the eight most active extracts were derived from ribes nigrum (ic50 of 27.7 μg/ml), ononis spinosa (ic50 of 39.4 μg/ml), urtica ... | 2015 | 26287155 |
| enhancement of anti-candidal activity of endophytic fungus phomopsis sp. ed2, isolated from orthosiphon stamineus benth, by incorporation of host plant extract in culture medium. | this study examined the effect of host extract in the culture medium on anti-candidal activity of phomopsis sp. ed2, previously isolated from the medicinal herb orthosiphon stamineus benth. interestingly, upon addition of aqueous host extract to the culture medium, the ethyl acetate extract prepared from fermentative broth exhibited moderate anti-candidal activity in a disc diffusion assay. the minimal inhibitory concentration of this extract was 62.5 μg/ml and it only exhibited fungistatic acti ... | 2012 | 22923105 |
| discovery of potential anti-infectives against staphylococcus aureus using a caenorhabditis elegans infection model. | the limited antibiotic options for effective control of methicillin-resistant staphylococcus aureus infections has led to calls for new therapeutic approaches to combat this human pathogen. an alternative approach to control mrsa is through the use of anti-infective agents that selectively disrupt virulence-mediated pathways without affecting microbial cell viability or by modulating the host natural immune defenses to combat the pathogen. | 2014 | 24393217 |
| orthosiphon stamineus protects caenorhabditis elegans against staphylococcus aureus infection through immunomodulation. | amidst growing concerns over the spread of antibiotic-resistant staphylococcus aureus strains, the identification of alternative therapeutic molecules has become paramount. previously, we utilized a caenorhabditis elegans-s. aureus screening platform to identify potential anti-infective agents from a collection of natural extracts and synthetic compounds. one of the hits obtained from the screen was the aqueous extract of orthosiphon stamineus leaves (ue-12) that enhanced the survival of infecte ... | 2014 | 24972867 |