| production of antiserum to a non-structural potyviral protein and its use to detect narcissus yellow stripe and other potyviruses. | a protein, of apparent molecular weight 72,000, was purified from experimentally infected narcissus plants with yellow stripe symptoms utilising sds-polyacrylamide gel electrophoresis. this protein was excised from the gels and used to prepare antiserum, which reacted specifically with cytoplasmic cylindrical inclusions in ultra-thin sections of virus-infected cells and, in immunoblots, with the 72 kda protein in preparations containing cytoplasmic inclusions. the antiserum reacted in elisa with ... | 1989 | 2778031 |
| nucleotide sequence of the coat protein genes of alstroemeria mosaic virus and amazon lily mosaic virus, a tentative species of genus potyvirus. | the nucleotide sequences of the 3' terminal region of the genomes of alstroemeria mosaic virus (alsmv) and the amazon lily mosaic virus (alimv) have been determined. these sequences contain the complete coding region of the viral coat protein (cp) gene followed by a 3'-untranslated region (3'-utr). alsmv and alimv share 74.9% identity in the amino acid sequence of the cp, and 55.6% identity in the nucleotide sequence of the 3'-utr. phylogenetic analysis of these cp genes and 3'-utrs in relation ... | 2004 | 15593424 |