Publications

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viruses recovered from mosquitoes and wildlife serum collected in the murray valley of south-eastern australia, february 1974, during an epidemic of encephalitis.pools of mosquitoes collected in the murray valley in february, 1974, during an encephalitis epidemic yielded 239 isolates of 11 distinct viruses. these included 39 isolates of mve virus, an incriminated causative agent of encephalitis in man, and 111 isolates of kunjin virus, a probable causative agent. an additional isolate of mve virus was recovered from the serum of a white-faced heron, ardea novaehollandiae. the other 9 viruses comprised the alpha-viruses ross river and sindbis, the flavivi ...19826299258
vector competence of mosquitoes (diptera: culicidae) from maroochy shire, australia, for barmah forest virus.mosquitoes were collected in light traps from maroochy shire and fed on blood containing the sympatric bf1611 strain of barmah forest virus (bf). saltmarsh aedes vigilax (skuse) and freshwater aedes procax (skuse) were highly susceptible to infection, with id50s of 10(1.7) and 10(1.5) african green monkey kidney (vero) cell culture infectious dose, 50% endpoint (ccid50) per mosquito, respectively, followed by aedes multiplex (theobald) and aedes funereus (theobald) with 10(2.5) and 10(3.2) ccid5 ...199910593091
mosquito (diptera: culicidae) dispersal: implications for the epidemiology of japanese and murray valley encephalitis viruses in australia.one hypothesis to explain the southern extension of japanese encephalitis (je) virus from papua new guinea into the torres strait islands in 1995 and to mainland australia in 1998 is the dispersal of infected mosquitoes, particularly culex annulirostris skuse from which je virus has been isolated repeatedly. to investigate whether this species disperses in this manner, mosquitoes were identified from 368 aerial kite trap collections operated at 50-310 m (altitude) at inland new south wales betwe ...200011126532
definition of ross river virus vectors at maroochy shire, australia.evidence of ross river (rr) virus infection in field-collected mosquitoes and data from laboratory vector competence experiments incriminated a range of mosquito species as important vectors of rr virus in maroochy shire, queensland, australia. nine rr and 2 barmah forest virus isolates were recovered from 27,529 mosquitoes collected in light traps from maroochy shire during 1996. nine of the 10 most abundant mosquito species collected in light traps were fed on blood containing the b94/20 strai ...200015218919
entomopathogenic fungi for mosquito control: a review.fungal diseases in insects are common and widespread and can decimate their populations in spectacular epizootics. virtually all insect orders are susceptible to fungal diseases, including dipterans. fungal pathogens such as lagenidium, coelomomyces and culicinomyces are known to affect mosquito populations, and have been studied extensively. there are, however, many other fungi that infect and kill mosquitoes at the larval and/or adult stage. the discovery, in 1977, of the selective mosquito-pa ...200415861235
mycobacterium ulcerans in mosquitoes captured during outbreak of buruli ulcer, southeastern australia.buruli ulcer (bu) occurs in >30 countries. the causative organism, mycobacterium ulcerans, is acquired from the environment, but the exact mode of transmission is unknown. we investigated an outbreak of bu in a small coastal town in southeastern australia and screened by pcr mosquitoes caught there. all cases of bu were confirmed by culture or pcr. mosquitoes were trapped in multiple locations during a 26-month period. bu developed in 48 residents of point lonsdale/queenscliff and 31 visitors fr ...200718217547
blood feeding patterns of mosquitoes: random or structured?abstract:201020205866
"Bird biting" mosquitoes and human disease: a review of the role of Culex pipiens complex mosquitoes in epidemiology.The transmission of vector-borne pathogens is greatly influenced by the ecology of their vector, which is in turn shaped by genetic ancestry, the environment, and the hosts that are fed on. One group of vectors, the mosquitoes in the Culex pipiens complex, play key roles in the transmission of a range of pathogens including several viruses such as West Nile and St. Louis encephalitis viruses, avian malaria (Plasmodium spp.), and filarial worms. The Cx. pipiens complex includes Culex pipiens pipi ...201121875691
a review of the status and significance of the species within the culex pipiens group in australia.the mosquito known in the northern hemisphere as culex pipiens (a.k.a. culex pipiens pipiens or culex pipiens form pipiens) is not known from australia. however, there are four species of the culex pipiens group: two indigenous, culex australicus and culex globocoxitus, and two introduced, culex quinquefasciatus and what is known locally as culex molestus (? = culex pipiens molestus or culex pipiens form molestus), all four being members of the pipiens subgroup (= 'pipiens complex'). the species ...201223401942
enhanced arbovirus surveillance with deep sequencing: identification of novel rhabdoviruses and bunyaviruses in australian mosquitoes.viral metagenomics characterizes known and identifies unknown viruses based on sequence similarities to any previously sequenced viral genomes. a metagenomics approach was used to identify virus sequences in australian mosquitoes causing cytopathic effects in inoculated mammalian cell cultures. sequence comparisons revealed strains of liao ning virus (reovirus, seadornavirus), previously detected only in china, livestock-infecting stretch lagoon virus (reovirus, orbivirus), two novel dimarhabdov ...201324314645
mosquito consumption by insectivorous bats: does size matter?insectivorous bats have often been touted as biological control for mosquito populations. however, mosquitoes generally represent only a small proportion of bat diet. given the small size of mosquitoes, restrictions imposed on prey detectability by low frequency echolocation, and variable field metabolic rates (fmr), mosquitoes may not be available to or profitable for all bats. this study investigated whether consumption of mosquitoes was influenced by bat size, which is negatively correlated w ...201324130851
role of enhanced vector transmission of a new west nile virus strain in an outbreak of equine disease in australia in 2011.in 2011, a variant of west nile virus kunjin strain (wnvkun) caused an unprecedented epidemic of neurological disease in horses in southeast australia, resulting in almost 1,000 cases and a 9% fatality rate. we investigated whether increased fitness of the virus in the primary vector, culex annulirostris, and another potential vector, culex australicus, contributed to the widespread nature of the outbreak.201425499981
ecological distribution and cq11 genetic structure of culex pipiens complex (diptera: culicidae) in italy.mosquitoes in the culex pipiens complex are considered to be involved in the transmission of a range of pathogens, including west nile virus (wnv). although its taxonomic status is still debated, the complex includes species, both globally distributed or with a more limited distribution, morphologically similar and characterised by different physiological and behavioural traits, which affect their ability as vectors. in many european countries, cx. pipiens and its sibling species culex torrentiu ...201626741494
using next-generation sequencing for dna barcoding: capturing allelic variation in its2.internal transcribed spacer 2 (its2) is a popular dna barcoding marker; however, in some animal species it is hypervariable and therefore difficult to sequence with traditional methods. with next-generation sequencing (ngs) it is possible to sequence all gene variants despite the presence of single nucleotide polymorphisms (snps), insertions/deletions (indels), homopolymeric regions, and microsatellites. our aim was to compare the performance of sanger sequencing and ngs amplicon sequencing in c ...201727799340
molecular identification of mosquitoes (diptera: culicidae) in southeastern australia.dna barcoding is a modern species identification technique that can be used to distinguish morphologically similar species, and is particularly useful when using small amounts of starting material from partial specimens or from immature stages. in order to use dna barcoding in a surveillance program, a database containing mosquito barcode sequences is required. this study obtained cytochrome oxidase i (coi) sequences for 113 morphologically identified specimens, representing 29 species, six trib ...201627217948
detection of theileria orientalis genotypes in haemaphysalis longicornis ticks from southern australia.theileria are blood-borne intracellular protozoal parasites belonging to the phylum apicomplexa. previously considered a benign parasite in australia, outbreaks of clinical disease resulting from theileria orientalis genotypes have been reported in australia since 2006. since this time, outbreaks have become widespread in south-eastern australia, resulting in significant adverse impacts on local dairy and beef industries. this paper provides the first investigation into the possible biological a ...201525889204
electron microscope detection of tubular aggregates in the mosquito cell line c6/36 treated with culex australicus (mosquito) homogenate.'tubular aggregates' are morphologically distinct cytoplasmic structures that have been linked to a variety of pathological conditions. this report documents the presence of tubular aggregates in an insect cell line (c6/36 cells derived from aedes albopictus) following inoculation of the cells with material derived from cell culture passaged homogenized culex australicus mosquitoes. the tubular aggregates were detected in ∼2% of treated cells and had three morphological forms that were termed pr ...201526323477
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