Publications
| cloning of circular dnas from microorganisms using a novel plasmid capture system. | plasmid capture system (pcs) facilitates cloning and manipulation of circular double-stranded dna. we recently developed an improved pcs (pcs-lz) to clone relatively large dna molecules of 30-150 kb. the pcs-lz donor consists of a mini-f replicon and a kanamycin resistance marker between tn7 left and tn7 right ends. both the replicon and marker gene of the pcs-lz donor are transferred into target plasmid dnas by in vitro transposition, followed by replication in e. coli. colonies are tested for ... | 2010 | 19838822 |
| identification of three host translation inhibitory factors encoded in cotesia glomerata bracovirus. | an endoparasitoid wasp, cotesia glomerata (braconidae: hymenoptera), parasitizes larvae of the diamondback moth, plutella xylostella (yponomeutidae: lepidoptera). parasitized p. xylostella exhibits immunosuppression and developmental alteration. in this study, the parasitized larvae exhibited a marked suppression in storage proteins (sps) especially at sp1 out of three sps, although there was significant mrna level of sp1. in addition, the parasitization significantly inhibited spreading behavio ... | 2009 | 20403759 |