Publications

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three closely related herpesviruses are associated with fibropapillomatosis in marine turtles.green turtle fibropapillomatosis is a neoplastic disease of increasingly significant threat to the survivability of this species. degenerate pcr primers that target highly conserved regions of genes encoding herpesvirus dna polymerases were used to amplify a dna sequence from fibropapillomas and fibromas from hawaiian and florida green turtles. all of the tumors tested (n = 23) were found to harbor viral dna, whereas no viral dna was detected in skin biopsies from tumor-negative turtles. the tis ...19989657957
herpesvirus-associated papillomatosis in a green lizard.papillomatous skin lesions from a green lizard (lacerta viridis) were examined histologically, using electron microscopy and dna was isolated from the lesions for herpes-viral dna detection. histology confirmed the lesions to be squamous epithelial papillomas. using electron microscopy, no virus particles were detected. the specific sequence of herpesviral dna-directed dna polymerase (ec 2.7.7.7) was amplified using degenerate primers in a nested format. the 235-base-pair (bp) sequence was seque ...201020090040
development and validation of a quantitative pcr assay for detection of emydoidea herpesvirus 1 in free-ranging blanding's turtles (emydoidea blandingii).blanding's turtles (emydoidea blandingii), an endangered species in illinois, have experienced range-wide declines because of habitat degradation and fragmentation, predation, and road mortality. while ongoing studies are crucial to a thorough understanding of the natural history and demographics in these disjointed illinois populations, infectious disease threats have been largely unevaluated. herpesvirus outbreaks have been associated with high morbidity and mortality in populations of captive ...201829360549
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