| shewanella decolorationis sp. nov., a dye-decolorizing bacterium isolated from activated sludge of a waste-water treatment plant. | a highly efficient dye-decolorizing bacterium, strain s12t, was isolated from activated sludge of a textile-printing waste-water treatment plant in guangzhou, china. the cells were gram-negative and motile by means of a single polar flagellum. the strain was capable of anaerobic growth either by fermentation of glucose or by anaerobic respiration and utilized a variety of electron acceptors, including nitrate, iron oxide and thiosulfate. the physiological properties, tested by using the biolog g ... | 2005 | 15653901 |
| decolorization of anthraquinone dye by shewanella decolorationis s12. | a new species of genus shewanella, shewanella decolorationis s12, from activated sludge of a textile-printing wastewater treatment plant, can decolorize reactive brilliant blue k-gr, one kind of anthraquinone dye, with flocculation first. although s. decolorationis displayed good growth in an aerobic condition, color removal was the best in an anaerobic condition. for color removal, the most suitable ph values and temperatures were ph 6.0-8.0 and 30-37 degrees c under anaerobic culture. more tha ... | 2006 | 16160829 |
| effects of electron donors and acceptors on anaerobic reduction of azo dyes by shewanella decolorationis s12. | shewanella decolorationis s12 was able to reduce various azo dyes in a defined medium with formate, lactate, and pyruvate or h(2) as electron donors under anaerobic conditions. purified membranous, periplasmic, and cytoplasmic fractions from strain s12 analyzed, respectively, only membranous fraction was capable of reducing azo dye in the presence of electron donor, indicating that the enzyme system for anaerobic azoreduction was located on cellular membrane. respiratory inhibitor cu(2+), dicuma ... | 2007 | 17043816 |
| respiration and growth of shewanella decolorationis s12 with an azo compound as the sole electron acceptor. | the ability of shewanella decolorationis s12 to obtain energy for growth by coupling the oxidation of various electron donors to dissimilatory azoreduction was investigated. this microorganism can reduce a variety of azo dyes by use of formate, lactate, pyruvate, or h(2) as the electron donor. furthermore, strain s12 grew to a maximal density of 3.0 x 10(7) cells per ml after compete reduction of 2.0 mm amaranth in a defined medium. this was accompanied by a stoichiometric consumption of 4.0 mm ... | 2007 | 17085710 |
| shewanella spongiae sp. nov., isolated from a marine sponge. | a psychrophilic bacterium, designated strain hj039(t), was isolated from a marine sponge collected in the east sea of korea (also known as the sea of japan). cells were gram-negative, motile and rod-shaped (1.8-3.54 microm x 0.27-0.73 microm). growth was observed between 5 and 26 degrees c (optimum 15 degrees c), at ph 5.0-8.5 (optimum ph 6.0-6.5) and in the presence of 0-6.0 % nacl (optimum 2.0 %). the 16s rrna gene sequence of strain hj039(t) showed high levels of similarity (93.7-95.4 %) with ... | 2006 | 17158991 |
| fe(iii)-enhanced azo reduction by shewanella decolorationis s12. | shewanella decolorationis s12 is capable of high rates of azo dye decolorization and dissimilatory fe(iii) reduction. under anaerobic conditions, when fe(iii) and azo dye were copresent in s12 cultures, dissimilatory fe(iii) reduction and azo dye biodecolorization occurred simultaneously. furthermore, the dye decolorization was enhanced by the presence of fe(iii). when 1 mm fe(iii) was added, the methyl red decolorizing efficiency was 72.1% after cultivation for 3 h, whereas the decolorizing eff ... | 2007 | 17216448 |
| reduction and partial degradation mechanisms of naphthylaminesulfonic azo dye amaranth by shewanella decolorationis s12. | reduction and biodegradation mechanisms of naphthylaminesulfonic azo dye amaranth using a newly isolated shewanella decolorationis strain s12 were investigated. under anaerobic conditions, amaranth was reduced by strain s12, and a stoichiometric amount of two reduction products rp-1 and rp-2 were generated. uv/visible spectrophotometric and high performance liquid chromatography (hplc) analysis indicated that rp-1 and rp-2 were 1-aminenaphthylene -4-sulfonic acid and 1-aminenaphthylene-2-hydroxy ... | 2007 | 17260140 |
| biodegradation of textile azo dye by shewanella decolorationis s12 under microaerophilic conditions. | the complete biodegradation of azo dye, fast acid red gr, was observed under microaerophilic conditions by shewanella decolorationis s12. although the highest decolorizing rate was measured under anaerobic condition and the highest biomass was obtained under aerobic condition, a further biodegradation of decolorizing products can only be achieved under microaerophilic conditions. under microaerophilic conditions, s. decolorationis s12 could use a range of carbon sources for azo dye decolorizatio ... | 2007 | 17589840 |
| humic substances act as electron acceptor and redox mediator for microbial dissimilatory azoreduction by shewanella decolorationis s12. | the potential for humic substances to serve as terminal electron acceptors in microbial respiration and the effects of humic substances on microbial azoreduction were investigated. the dissimilatory azoreducing microorganism shewanella decolorationis s12 was able to conserve energy to support growth from electron transport to humics coupled to the oxidation of various organic substances or h2. batch experiments suggested that when the concentration of anthraquinone-2-sulfonate (aqs), a humics an ... | 2007 | 18050946 |
| biodegradation of crystal violet by a shewanella sp. ntou1. | a bacterial isolate, strain ntou1, originally isolated from the cooling system in an oil refinery could decolorize and detoxify crystal violet under anaerobic conditions. the strain was characterized and identified as a member of shewanella decolorationis based on gram staining, morphology characters, biochemical tests, the 16s rrna gene and the gyrase subunit beta gene (gyrb). the optimum ph value and temperature for decolorization of crystal violet by this strain under anaerobic conditions wer ... | 2008 | 18582917 |
| identification of an uptake hydrogenase for hydrogen-dependent dissimilatory azoreduction by shewanella decolorationis s12. | shewanella decolorationis s12, a representative dissimilatory azo-reducing bacterium of shewanella genus, can grow by coupling the oxidation of hydrogen to the reduction of azo compounds as the sole electron acceptor, indicating that an uptake hydrogenase is an important component for electron transfer for azoreduction. for searching to the uptake hydrogenase in the genome of s. decolorationis, two operons, hyd and hya, were cloned and sequenced, which encode periplasmically oriented fe-only hyd ... | 2008 | 18651140 |
| energy generation coupled to azoreduction by membranous vesicles from shewanella decolorationis s12. | previous studies have demonstrated that shewanella decolorationis s12 can grow on the azo compound amaranth as the sole electron acceptor. thus, to explore the mechanism of energy generation in this metabolism, membranous vesicles (mvs) were prepared and the mechanism of energy generation investigated. the membrane, which was fragmentized during preparation, automatically formed vesicles ranging from 37.5-112.5 nm in diameter under electron micrograph observation. energy was conserved when coupl ... | 2009 | 19190406 |
| effects of oxytetracycline and sulfachloropyridazine residues on the reductive activity of shewanella decolorationis s12. | effects of oxytetracycline (otc) and sulfachloropyridazine (scp), two of the widely used antibiotics in livestock production, on beneficial environmental microorganisms were studied. shewanella decolorationis s12 was selected as the target bacteria for the role in reduction of fe(iii) and dye under anaerobic conditions. the results showed that the antibiotics significantly inhibited fe(iii) reduction and dye decoloration in the reduction system. the rates of fe(ii) formed (-r) were 3.6 and 0.2 m ... | 2009 | 19527026 |
| a polypyrrole/anthraquinone-2,6-disulphonic disodium salt (ppy/aqds)-modified anode to improve performance of microbial fuel cells. | this study reports a new approach of improving performance of microbial fuel cells (mfcs) by using a polypyrrole/anthraquinone-2,6-disulphonic disodium salt (ppy/aqds)-modified anode. the immobilization of aqds on a carbon felt anode was accomplished by electropolymerization of pyrrole while using aqds as the dopant. the dual-chamber mfc operated with this modified anode in the presence of shewanella decolorationis s12 showed the maximum power density of 1303 mw m(-2), which was 13 times larger ... | 2010 | 19889528 |
| two different electron transfer pathways may involve in azoreduction in shewanella decolorationis s12. | electron transfer pathways for azoreduction by s. decolorationis s12 were studied using a mutant s12-22 which had a transposon insertion in ccma. the results imply that there are two different pathways for electron transport to azo bonds. the colony of s12-22 was whitish and incapable of producing mature c-type cytochromes whose alpha-peak was at 553 nm in the wild type s12. the mutant s12-22 could not use formate as the sole electron donor for azoreduction either in vivo or in vitro, but intact ... | 2010 | 20012540 |
| enhanced reductive dechlorination of ddt in an anaerobic system of dissimilatory iron-reducing bacteria and iron oxide. | the transformation of ddt was studied in an anaerobic system of dissimilatory iron-reducing bacteria (shewanella decolorationis s12) and iron oxide (alpha-feooh). the results showed that s. decolorationis could reduce ddt into ddd, and ddt transformation rate was accelerated by the presence of alpha-feooh. ddd was observed as the primary transformation product, which was demonstrated to be transformed in the abiotic system of fe(2+)+alpha-feooh and the system of dirb+alpha-feooh. the intermediat ... | 2010 | 20031285 |
| partial degradation mechanisms of malachite green and methyl violet b by shewanella decolorationis ntou1 under anaerobic conditions. | this work demonstrated that shewanella decolorationis ntou1 decolorized 200 mg l(-1) of crystal violet, malachite green, or methyl violet b within 2-11h under anaerobic conditions at 35 degrees c. the initial color removal rate of malachite green was highest, while that of methyl violet was lowest. gc/ms analyses of the intermediate compounds produced during and after decolorization of malachite green and methyl violet b suggested that biodegradation of these dyes involved reduction to leuco for ... | 2010 | 20060225 |
| [expression and characterization of the nonspecific azoreducase of shewanella decolorationis s12]. | in order to identify the function of acpd in azoreduction, the gene was obtained from shewanella decolorationis s12 and expressed in escherichia coli top10 which showed little azoreduction activity. | 2009 | 20069878 |
| bio-electro-fenton process driven by microbial fuel cell for wastewater treatment. | in this study, we proposed a new concept of utilizing the biological electrons produced from a microbial fuel cell (mfc) to power an e-fenton process to treat wastewater at neutral ph as a bioelectro-fenton (bio-e-fenton) process. this process can be achieved in a dual-chamber mfc from which electrons were generated via the catalyzation of shewanella decolorationis s12 in its anaerobic anode chamber and transferred to its aerated cathode chamber equipped with a carbon nanotube (cnt)/gamma-feooh ... | 2010 | 20108963 |
| comparative analysis of membranous proteomics of shewanella decolorationis s12 grown with azo compound or fe (iii) citrate as sole terminal electron acceptor. | shewanella decolorationis s12 is capable of carrying out anaerobic respiration using azo dyes and fe (iii) citrate as electron acceptors. in the present study, proteomic techniques including two-dimensional gel electrophoresis (2-de) and matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry were used to analyze the similarity and the dissimilarity of the membrane proteins isolated from strain s12 cells grown in amaranth or fe (iii) citrate with defined inorg ... | 2010 | 20309544 |
| [effects of iron on azoreduction by shewanella decolorationis s12]. | the effects of soluble and insoluble fe(iii) on anaerobic azoreduction by shewanella decolorationis s12 were examined in a series of experiments. results showed that the effects of iron on anaerobic azoreduction depended on the solubility and concentration of the compounds. azoreduction was inhibited by insoluble fe(iii) and 0.05-2 mmol/l fe2 o3 all decelerated the azoreduction activity of 0.2 mmol/l amaranth, but the increase in the concentrations of fe2o3 did not cause an increasing inhibition ... | 2010 | 20329544 |
| effects of oxygen on shewanella decolorationis ntou1 electron transfer to carbon-felt electrodes. | to clarify the major factor caused by oxygen-enhancing charge production of shewanella decolorationis ntou1 towards a polarized anode, a series of experimental runs (i.e., with/without ambient air flushing and with/without ammonia addition as nitrogen source) were conducted in this study. within 6-day of operation at +0.4 v vs. ag|agcl and starting with 35 mm of lactate, consistently the electrical charge production under the aerobic condition was higher than that under the anaerobic condition. ... | 2010 | 20494569 |
| role of iron in azoreduction by resting cells of shewanella decolorationis s12. | to investigate the role of soluble and insoluble iron in azoreduction by resting cells of shewanella decolorationis s12. | 2010 | 21159097 |
| differential biofilms characteristics of shewanella decolorationis microbial fuel cells under open and closed circuit conditions. | biofilms formation capacities of shewanella species in microbial fuel cells (mfcs) and their roles in current generation have been documented to be species-dependent. understandings of the biofilms growth and metabolism are essential to optimize the current generation of mfcs. shewanella decolorationis s12 was used in both closed-circuit and open-circuit mfcs in this study. the anodic s. decolorationis s12 biofilms could generate fivefold more current than the planktonic cells, playing a dominan ... | 2011 | 21571526 |
| electron acceptor redox potential globally regulates transcriptomic profiling in shewanella decolorationis s12. | electron acceptor redox potential (earp) was presumed to be a determining factor for microbial metabolism in many natural and engineered processes. however, little is known about the potentially global effects of earp on bacteria. in this study, we compared the physiological and transcriptomic properties of shewanella decolorationis s12 respiring with different earps in microbial electrochemical systems to avoid the effects caused by the other physicochemical properties of real electron acceptor ... | 2016 | 27503002 |
| characteristics and kinetic analysis of aqs transformation and microbial goethite reduction:insight into "redox mediator-microbe-iron oxide" interaction process. | the characteristics and kinetics of redox transformation of a redox mediator, anthraquinone-2-sulfonate (aqs), during microbial goethite reduction by shewanella decolorationis s12, a dissimilatory iron reduction bacterium (dirb), were investigated to provide insights into "redox mediator-iron oxide" interaction in the presence of dirb. two pre-incubation reaction systems of the "strain s12- goethite" and the "strain s12-aqs" were used to investigate the dynamics of goethite reduction and aqs red ... | 2016 | 27020166 |
| from red to green: the propidium iodide-permeable membrane of shewanella decolorationis s12 is repairable. | viability is a common issue of concern in almost all microbial processes. fluorescence-based assays are extensively used in microbial viability assessment, especially for mixed-species samples or biofilms. propidium iodide (pi) is the most frequently used fluorescence indicator for cell viability based on the membrane permeability. our results showed that the accumulation of succinate from fumarate respiration could induce pi-permeability in shewanella decolorationis biofilm cells. confocal lase ... | 2015 | 26687136 |
| characterization of the enhancement of zero valent iron on microbial azo reduction. | the microbial method for the treatment of azo dye is promising, but the reduction of azo dye is the rate-limiting step. zero valent iron (fe(0)) can enhance microbial azo reduction, but the interactions between microbes and fe(0) and the potential mechanisms of enhancement remain unclear. here, shewanella decolorationis s12, a typical azo-reducing bacterium, was used to characterize the enhancement of fe(0) on microbial decolorization. | 2015 | 25888062 |
| electron acceptor-dependent respiratory and physiological stratifications in biofilms. | bacterial respiration is an essential driving force in biogeochemical cycling and bioremediation processes. electron acceptors respired by bacteria often have solid and soluble forms that typically coexist in the environment. it is important to understand how sessile bacteria attached to solid electron acceptors respond to ambient soluble alternative electron acceptors. microbial fuel cells (mfcs) provide a useful tool to investigate this interaction. in mfcs with shewanella decolorationis, azo ... | 2015 | 25495895 |
| the characteristics and two-step reaction model of p-nitroacetophenone biodegradation mediated by shewanella decolorationis s12 and electron shuttle in the presence/absence of goethite. | the current study mainly focused on the biodegradation process of p-nitroacetophenone (np) in the presence and absence of goethite mediated by iron-reducing microbe (shewanella decolorationis s12) and electron shuttle. the results showed that introduction of electron shuttle could obviously lead to an accumulation of biodegradation intermediate, especially in reaction systems containing high content of electron shuttle in the absence of goethite. goethite could enhance the degree and rate of np ... | 2014 | 25244139 |
| physiological and electrochemical effects of different electron acceptors on bacterial anode respiration in bioelectrochemical systems. | to understand the interactions between bacterial electrode respiration and the other ambient bacterial electron acceptor reductions, alternative electron acceptors (nitrate, fe2o3, fumarate, azo dye mb17) were added singly or multiply into shewanella decolorationis microbial fuel cells (mfcs). all the added electron acceptors were reduced simultaneously with current generation. adding nitrate or mb17 resulted in more rapid cell growth, higher flavin concentration and higher biofilm metabolic via ... | 2014 | 24862003 |
| draft genome sequence of shewanella decolorationis s12, a dye-degrading bacterium isolated from a wastewater treatment plant. | shewanella decolorationis is a valuable microorganism for degrading diverse synthetic textile dyes. here, we present an annotated draft genome sequence of s. decolorationis s12, which contains 4,219 protein-coding genes and 86 structural rnas. this information regarding the genetic basis of this bacterium can greatly advance our understanding of the physiology of this species. | 2013 | 24309738 |
| [zero-valent iron-enhanced azoreduction by the shewanella decolorationis s12]. | characteristics and optimal reaction conditions of anaerobic azoreduction by the shewanella decolorationis s12 in the presence of zero-valent iron (zvi) were evaluated in this study. the results showed that the presence of zvi significantly enhanced the decolorization rate of azo dye. in the presence of 20 mmol x l(-1) zvi, the decolorization rate of 1 mmol x l(-1) amaranth reached up to 100% by the shewanella decolorationis s12 after 30 h incubation, which was significantly higher than 23.16% a ... | 2013 | 24028023 |
| characterizing the snorkeling respiration and growth of shewanella decolorationis s12. | microbial electrochemical snorkel (mes) reactor is a simplified bioreactor based on microbial fuel cells (mfcs) and has been suggested to be a promising approach to solve many environmental problems. however, the microbial processes in mes reactors have not yet been characterized. this study shows that shewanella decolorationis s12 can use the conductive snorkel as direct electron acceptor for respiration and growth. similar with current-generating biofilms, cellular viability in mes biofilms de ... | 2013 | 23201531 |
| shewanella seohaensis sp. nov., isolated from a tidal flat sediment. | a gram-negative, motile and rod-shaped bacterial strain, designated s7-3(t), was isolated from a tidal flat sediment at saemankum on the western coast of korea. phylogenetic analyses based on 16s rrna gene and gyrb sequences showed that strain s7-3(t) belonged to the genus shewanella, clustering with shewanella decolorationis s12(t). strain s7-3(t) exhibited 98.8 % 16s rrna gene sequence similarity and 96.8 % gyrb sequence similarity to s. decolorationis s12(t), respectively. the 16s rrna gene s ... | 2012 | 22437441 |
| electricity generation by shewanella decolorationis s12 without cytochrome c. | bacterial extracellular electron transfer (eet) plays a key role in various natural and engineering processes. outer membrane c-type cytochromes (omcs) are considered to be essential in bacterial eet. however, most bacteria do not have omcs but have redox proteins other than omcs in their extracellular polymeric substances of biofilms. we hypothesized that these extracellular non-cytochrome c proteins (encp) could contribute to eet, especially with the facilitation of electron mediators. this st ... | 2017 | 28676795 |