Publications

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the lethal and hepatocarcinogenic effects of dimethylnitrosamine injection in the newt triturus helveticus. 19725047143
palmate newt mortality at an english breeding site. 200312733567
[modifications induced by ultrasonics on the structure of striated muscles of the larvae of bufo bufo l. and of triturus helveticus raz]. 195513305076
tetrodotoxin and its analogue 6-epitetrodotoxin in newts (triturus spp.; urodela, salamandridae) from southern germany.tetrodotoxin (ttx) and its analogue 6-epitetrodotoxin (6-epittx) were quantitatively assayed in 59 newts representing four triturus species (triturus alpestris, triturus cristatus, triturus helveticus, triturus vulgaris) from southern germany by a post-column fluorescent-hplc system. both toxins were detected in only 15 specimens of the four species. the toxins levels varied considerably among individuals (ttx: 0.11-9.0microg/g; 6-epittx: 0.05-17.0microg/g). 6-epittx was found to be the major co ...200717507070
[action of caproic and caprylic acids on segmentation mitoses of triturus helveticus razoumowsky]. 20164734148
[vitellogenesis in the ovocyte of the triturus helveticus raz. ultrastructural study]. 20144335977
[a newt (triturus helveticus) with black eyes]. 20164802818
experimental evidence for a cost of resistance to the fungal pathogen, batrachochytrium dendrobatidis, for the palmate newt, lissotriton helveticus.batrachochytrium dendrobatidis (bd), the causative agent of chytridiomycosis, is decimating amphibians worldwide. unsurprisingly, the majority of studies have therefore concentrated on documenting morbidity and mortality of susceptible species and projecting population consequences as a consequence of this emerging infectious disease. currently, there is a paucity of studies investigating the sub-lethal costs of bd in apparently asymptomatic species, particularly in controlled experimental condi ...201323866033
impact of both desiccation and exposure to an emergent skin pathogen on transepidermal water exchange in the palmate newt lissotriton helveticus .amphibians are the vertebrate group most affected by global change. their highly permeable skin is involved in maintaining homeostasis (e.g. water and electrolyte equilibrium), which makes them particularly vulnerable to climate warming and skin pathogens. this study focused on the impacts of both desiccation (as a potential consequence of climate warming) and exposure to batrachochytrium dendrobatidis (bd), an emergent skin pathogen of amphibians. bd causes chytridiomycosis, a lethal skin disea ...201323759559
pathological and phylogenetic characterization of amphibiothecum sp. infection in an isolated amphibian (lissotriton helveticus) population on the island of rum (scotland).outbreaks of cutaneous infectious disease in amphibians are increasingly being attributed to an overlooked group of fungal-like pathogens, the dermocystids. during the last 10 years on the isle of rum, scotland, palmate newts (lissotriton helveticus) have been reportedly afflicted by unusual skin lesions. here we present pathological and molecular findings confirming that the pathogen associated with these lesions is a novel organism of the order dermocystida, and represents the first formally r ...201727873566
[relative antigenicity of tegument embryonic heterografts in urodele amphibians: ambystoma mexicanum shaw, triturus helveticus raz., triturus alpestris laur. and pleurodeles waltlii michah]. 19694979133
what remains from a 454 run: estimation of success rates of microsatellite loci development in selected newt species (calotriton asper, lissotriton helveticus, and triturus cristatus) and comparison with illumina-based approaches.the development of microsatellite loci has become more efficient using next-generation sequencing (ngs) approaches, and many studies imply that the amount of applicable loci is large. however, few studies have sought to quantify the number of loci that are retained for use out of the thousands of sequence reads initially obtained. we analyzed the success rate of microsatellite loci development for three amphibian species using a 454 ngs approach on tetra-nucleotide motif-enriched species-specifi ...201324198952
divergence of species-specific protein sex pheromone blends in two related, nonhybridizing newts (salamandridae).in animals that use chemical communication during courtship and reproduction, speciation is often associated with divergence of their sex pheromones. in multicomponent pheromone systems, divergence can be obtained either by adding or deleting components, or by altering the relative contribution of individual components to the mixture. protein pheromone systems can additionally evolve by amino acid sequence divergence to produce pheromones with a species-specific effect. the sodefrin precursor-li ...201729087032
skin microbiota differs drastically between co-occurring frogs and newts.diverse microbial assemblages inhabit amphibian skin and are known to differ among species; however, few studies have analysed these differences in systems that minimize confounding factors, such as season, location or host ecology. we used high-throughput amplicon sequencing to compare cutaneous microbiotas among two ranid frogs (rana dalmatina, r. temporaria) and four salamandrid newts (ichthyosaura alpestris, lissotriton helveticus, l. vulgaris, triturus cristatus) breeding simultaneously in ...201728484639
love is blind: indiscriminate female mating responses to male courtship pheromones in newts (salamandridae).internal fertilization without copulation or prolonged physical contact is a rare reproductive mode among vertebrates. in many newts (salamandridae), the male deposits a spermatophore on the substrate in the water, which the female subsequently takes up with her cloaca. because such an insemination requires intense coordination of both sexes, male newts have evolved a courtship display, essentially consisting of sending pheromones under water by tail-fanning towards their potential partner. beha ...201323457580
effects of water contamination on site selection by amphibians: experiences from an arena approach with european frogs and newts.pesticide residues in breeding ponds can cause avoidance by at least some amphibian species. so far, outdoor experiments have been performed only with artificial pools in areas where the focus species usually occur and new colonization has been observed. results of this kind of study are potentially influenced by natural disturbances and therefore are of limited comparability. we used an easily manufactured and standardizable arena approach, in which animals in reproductive condition for some ho ...201323377318
[inversion of the sexual phenotype (feminization) of triturus helveticus raz. and triturus alpestris laur, following gynogenous treatment by estradiol benzoate administered during the larval stage]. 196013826151
widespread introgression does not leak into allotopy in a broad sympatric zone.species that overlap over a large part of their range and habitat requirements are challenging for the study of speciation and hybridization. in this respect, the study of broadscale introgressive hybridization has raised recent interest. here we studied hybridization between two closely related amphibians lissotriton helveticus and lissotriton vulgaris that reproduce over a wide sympatric zone. we used mitochondrial and microsatellite markers on 1272 individuals in 37 sites over europe to detec ...201121081968
a case of reproductive character displacement in female palmate newts (lissotriton helveticus).reproductive character displacement is commonly described as larger phenotypic differences between species living in sympatry rather than in allopatry. we investigated this phenomenon on two amphibians found in their contact zone where syntopic and allotopic sites alternate. to test the effect of syntopy with lissotriton vulgaris on the lissotriton helveticus phenotype, we studied the morphology of adult males and females while controlling for environmental factors (i.e. land use and flood risk) ...200919520318
effects of ph and aluminium on the growth and feeding behaviour of smooth and palmate newt larvae.: the distribution of smooth and palmate newts (triturus vulgaris and triturus helveticus) may be related to water quality. this study compared the larval growth and feeding behaviour of both species under sublethal levels of aluminium and low ph. the growth of both species was inhibited to a similar degree under acidic conditions with aluminium toxicity dependent upon both low ph and developmental stage. the two species were differentially affected by low ph in terms of feeding behaviour. t. vu ...199524197797
are there insertions in the ribosomal dna of vertebrates?we have analysed the eco ri restriction pattern of rdna of the newt triturus vulgaris and of some other amphibian species by southern blotting and hybridization with nick-translated xenopus rdna prepared from the recombinant plasmids pxlr11 and pxlr12 (21). after hybridization with r11, the 28s coding fragments become visible in two bands, a prominent one of 5.3 kb and a weak band of 5.9 kb representing about 8% of the 28s genes. the evidence obtained so far by additional digestions with bam hi ...19852991856
a reference dna barcode library for austrian amphibians and reptiles.in the last few years, dna barcoding became an established method for species identification in biodiversity inventories and monitoring studies. such studies depend on the access to a comprehensive reference data base, covering all relevant taxa. here we present a comprehensive dna barcode inventory of all amphibian and reptile species native to austria, except for the putatively extinct vipera ursinii rakosiensis and lissotriton helveticus, which has been only recently reported for the very wes ...202032163447
context-dependent dispersal, public information, and heterospecific attraction in newts.dispersal is one of the main processes that determine community structure. individuals make dispersal decisions according to environmental and/or social cues that reflect the fitness prospects in a given patch. the presence and abundance of heterospecifics within the same ecological guild, and/or their breeding success, may act as public information that influences movement decisions. to date, most studies investigating the role of heterospecific attraction have focused on habitat choice, using ...201830315372
mosquito control based on bacillus thuringiensis israelensis (bti) interrupts artificial wetland food chains.the biocide bacillus thuringiensis israelensis (bti) has become the most commonly used larvicide to control mosquitoes in seasonal wetlands. although bti is considered non-toxic to most aquatic organisms, the non-biting chironomids show high susceptibilities towards bti. as chironomids are a key element in wetland food webs, major declines in their abundance could lead to indirect effects that may be passed through aquatic and terrestrial food chains. we conducted two mesocosm experiments to add ...201931412513
skin mucosome activity as an indicator of batrachochytrium salamandrivorans susceptibility in salamanders.recently emerged fungal diseases, batrachochytrium dendrobatidis (bd) and batrachochytrium salamandrivorans (bsal) are an increasing threat to amphibians worldwide. in europe, the threat of bsal to salamander populations is demonstrated by the rapid decline of fire salamander populations in germany, the netherlands and belgium. although most european urodelans are susceptible to infection in infection trials, recent evidence suggests marked interspecific differences in the course of infection, w ...201830020936
a pheromone by any other gene would smell as sweet.reproductive isolation is the result of either the inability to produce viable and fertile offspring or the avoidance of mating altogether. while these mechanisms can evolve either over time via genetic drift or natural selection, the genetic result is usually a complex set of traits that are often linked. explaining how reproductive isolation proceeds from the initiation of divergence to the complete prevention of mating is often a difficult task, as the underlying genes for traits associated w ...201829561075
on the identification of paedomorphic and overwintering larval newts based on cloacal shape: review and guidelines.paedomorphosis is an alternative process to metamorphosis in which adults retain larval traits at the adult stage. it is frequent in newts and salamanders, where larvae reach sexual maturity without losing their gills. however, in some populations, larvae overwinter in water, while remaining immature. these alternative ontogenetic processes are of particular interest in various research fields, but have different causes and consequences, as only paedomorphosis allows metamorphosis to be bypassed ...201729491974
recent findings of potentially lethal salamander fungus batrachochytrium salamandrivorans.the distribution of the chytrid fungus batrachochytrium salamandrivorans continues to expand in europe. during 2014-2018, we collected 1,135 samples from salamanders and newts in 6 countries in europe. we identified 5 cases of b. salamandrivorans in a wild population in spain but none in central europe or the balkan peninsula.201931211934
reproductive fitness consequences of progenesis: sex-specific pay-offs in safe and risky environments.progenesis is considered to have an important role in evolution because it allows the retention of both a larval body size and shape in an adult morphology. however, the cost caused by the adoption of a progenetic process in both males and females remains to be explored to explain the success of progenesis and particularly its biased prevalence across the sexes and environments. here, through an experimental approach, we used a facultative progenetic species, the palmate newt (lissotriton helvet ...201930927549
the role of predation risk in metamorphosis versus behavioural avoidance: a sex-specific study in a facultative paedomorphic amphibian.evolutionary theory predicts the evolution of metamorphosis over paedomorphosis (the retention of larval traits at the adult stage) in response to life in unfavourable habitats and to the benefits of dispersal. although many organisms are canalised into obligatory complex or simple life cycles, some species of newts and salamanders can express both processes (facultative paedomorphosis). previous research highlighted the detrimental effect of fish on both metamorphic and paedomorphic phenotypes, ...201930809707
the 'male escape hypothesis': sex-biased metamorphosis in response to climatic drivers in a facultatively paedomorphic amphibian.paedomorphosis is a major evolutionary process that bypasses metamorphosis and allows reproduction in larvae. in newts and salamanders, it can be facultative with paedomorphs retaining gills and metamorphs dispersing. the evolution of these developmental processes is thought to have been driven by the costs and benefits of inhabiting aquatic versus terrestrial habitats. in this context, we aimed at testing the hypothesis that climatic drivers affect phenotypic transition and the difference acros ...201728424346
secondary compounds from exotic tree plantations change female mating preferences in the palmate newt (lissotriton helveticus).selection can favour phenotypic plasticity in mate choice in response to environmental factors that alter the costs and benefits of being choosy, or of choosing specific mates. human-induced environmental change could alter sexual selection by affecting the costs of mate choice, or by impairing the ability of individuals to identify preferred mates. for example, variation in mate choice could be driven by environmentally induced differences in body condition (e.g. health) that change the cost of ...201728419678
habitat-related variation in the plasticity of a uv-sensitive photoreceptor over a small spatial scale in the palmate newt.plastic phenotypes are expected to be favoured in heterogeneous environments compared with stable environments. sensory systems are interesting to test this theory because they are costly to produce and support, and strong fitness costs are expected if they are not tuned to the local environment. consistently, the visual system of several species changes with the conditions experienced during early development. however, there is little information on whether the amplitude of the change, that is ...201728370602
high gene flow between alternative morphs and the evolutionary persistence of facultative paedomorphosis.paedomorphosis and metamorphosis are two major developmental processes that characterize the evolution of complex life cycles in many lineages. whereas these processes were fixed in some taxa, they remained facultative in others, with alternative phenotypes expressed in the same populations. from a genetic perspective, it is still unknown whether such phenotypes form a single population or whether they show some patterns of isolation in syntopy. this has deep implications for understanding the e ...201627534370
condition-dependent trade-offs between sexual traits, body condition and immunity: the effect of novel habitats.the optimal allocation of resources to sexual signals and other life history traits is usually dependent on an individual's condition, while variation in the expression of sexual traits across environments depends on the combined effects of local adaptation, mean condition, and phenotypic responses to environment-specific cues that affect resource allocation. a clear contrast can often be drawn between natural habitats and novel habitats, such as forest plantations and urban areas. in some speci ...201627329759
intra-specific variability of hindlimb length in the palmate newt: an indicator of population isolation induced by habitat fragmentation?habitat fragmentation is one of the main drivers of global amphibian decline. anthropogenic landscape elements can act as barriers, hindering the dispersal that is essential for maintaining gene flow between populations. dispersal ability can be influenced by locomotor performance, which in turn can depend on morphological traits, such as hindlimb length (hll) in amphibians. here, we tested relationships between hll and environmental variables--road types, forests and agricultural lands--among 3 ...201627122009
using kernels and ecological niche modeling to delineate conservation areas in an endangered patch-breeding phenotype.efficient delineation of conservation areas is a great challenge in maintaining biodiversity. kernel density estimators (kdes) are a powerful tool in this perspective, but they have not been applied at the population level on patch-distributed organisms. this would be particularly worthy for species that need broad habitats beyond those where they can be sampled; such as terrestrial lands for pond-breeding amphibians. the aim of this study was to compare different approaches for the identificati ...201526591457
evidence of light-dependent magnetic compass orientation in urodele amphibian larvae.experiments were conducted to investigate whether larval palmate newts undertake orientation toward or away from the home shoreline (y-axis orientation) using the geomagnetic field to steer the most direct route, and if they accomplish this task through a light-dependent magnetoreception mechanism similar to that found in anuran tadpoles and adult newts. larval palmate newts trained and then tested under full-spectrum light showed bimodal magnetic compass orientation that coincided with the magn ...201525981491
expression of sexual ornaments in a polymorphic species: phenotypic variation in response to environmental risk.secondary sexual traits may evolve under the antagonistic context of sexual and natural selection. in some polymorphic species, these traits are only expressed during the breeding period and are differently expressed in alternative phenotypes. however, it is unknown whether such phenotypes exhibit phenotypic plasticity of seasonal ornamentations in response to environmental pressures such as in the presence of fish (predation risk). this is an important question to understand the evolution of po ...201525847588
side-by-side secretion of late palaeozoic diverged courtship pheromones in an aquatic salamander.males of the advanced salamanders (salamandroidea) attain internal fertilization without a copulatory organ by depositing a spermatophore on the substrate in the environment, which females subsequently take up with their cloaca. the aquatically reproducing modern eurasian newts (salamandridae) have taken this to extremes, because most species do not display close physical contact during courtship, but instead largely rely on females following the male track at spermatophore deposition. although ...201525694622
information-content of morphological and behavioural sexual traits in the palmate newt (lissotriton helveticus).the question of why females evaluate more than one sexual trait to choose their mates has received increasing attention in recent years. here, we investigated the information-content of both morphological and behavioural sexual traits that have been identified as predictors of male reproductive success in the palmate newt, lissotriton helveticus. we examined the co-variation of multiple traits with one aspect of male quality, the male body condition, using both a correlative study and an experim ...201425241307
heterochrony in a complex world: disentangling environmental processes of facultative paedomorphosis in an amphibian.heterochrony, the change in the rate or timing of development between ancestors and their descendants, plays a major role in evolution. when heterochrony produces polymorphisms, it offers the possibility to test hypotheses that could explain its success across environments. amphibians are particularly suitable to exploring these questions because they express complex life cycles (i.e. metamorphosis) that have been disrupted by heterochronic processes (paedomorphosis: retention of larval traits i ...201424180255
evolutionary history of lissotriton helveticus: multilocus assessment of ancestral vs. recent colonization of the iberian peninsula.the pleistocene was characterized by climatic changes that greatly altered the distribution of organisms. population extinctions, bottlenecks, isolation, range expansions and contractions were often associated with glaciations, leaving signatures in the spatial patterns of genetic diversity across species. lissotriton helveticus belongs to a pan-european lineage of newts that were strongly affected by glaciations and represent an excellent model to analyse the effect of generalized climatic chan ...201121530664
dermocystid infection and associated skin lesions in free-living palmate newts (lissotriton helveticus) from southern france.since the early 1900s, mesomycetozoan parasites have been reported in both european anuran and caudate species. these reports have primarily been descriptive, which has made assessing the impact of these parasites on host populations difficult. anecdotal reports of dermocystidium-like parasites are becoming widespread across europe, possibly indicating that these mesomycetozoan parasites are increasing in distribution and/or abundance. this highlights the need for further investigations into the ...201020450984
habitat partitioning in facultatively paedomorphic populations of palmate newts triturus helveticus. 200516201220
[thyroid incorporation of radioactive iodine in neotenic tritons (triturus helveticus raz.). in vivo studies by high resolution radioautography. comparison of iodine incorporation in adults and larvae].in vivo kinetics of radioiodine incorporation have been established for 70 newts, collected from triturus helveticus raz. populations where spontaneous neoteny occurs. fifty of these newts which appeared to present four degrees of neoteny (more or less metamorphosed), have been studied. main results obtained were: 1) the intensity of the iodine incorporation varies according to the degree of neoteny. the higher is this one, the lower is the intensity. 2) in general, iodine uptake rates are more ...1979475306
golgi-staining of "primary" and "secondary" motoneurons in the developing spinal cord of an amphibian.the golgi technique was used to study the morphology of spinal motoneurons at various stages in the early development of swimming behaviour in embryos and larvae of the palmate newt, triturus helveticus ((razoumowsky). the earliest motoneurons stained appeared to be associated with the mauthner-cell system. the overall morphology of these "primary" motoneurons seems to be similar throughout the lower vertebrates and the distinctive characteristics found in earlier descriptions of those from caud ...1978681545
electrophoretic patterns of larval, neotenic and adult haemoglobin of triturus helveticus raz. 1978747079
[determination of the relative iodine concentration in the lumens of thyroid follicles of normal and neotenic triturus helveticus, and evaluation of the halogen content of the thyroid colloid. electron microprobe study (author's transl)].the stable, bound iodine in the thyroid colloid of triturus helveticus has been studied with the electron microprobe. some animals show a normal development. others are accidentally neotenic due to certain ecological conditions. the values of punctate iodine concentrations (cpi) have been computed and expressed in relative units (counts/s). the mean cpi per lumen is very variable from one follicle to another in the same thyroid section. during normal development, the mean cpi per animal is gener ...1977871209
[trifluralin, an inhibitor of the achromatic apparatus which modifies the chromosomes].trifluralin weakens or destroys polarity in segmentation mitoses of triturus helveticus raz. and pleurodeles waltlii michah. previously it inhibits the cytokinesis, by reducing the activity of asters which are necessary to it. it produces chromosome abnormalities, specially involving their condensation, and makes appear discontinuities at prophase. the local thickenings of metaphase chromosomes predominate at heterochromatic regions. the action is stronger in animal than in vegetative half and t ...1977567039
ultrastructure of segmentation mitoses in cleaving newt eggs after blocking of centrospheres by quinoline.in quinoline treated blastomeres of triturus helveticus raz. and pleurodeles waltlii michah., microtubules disappear and around the centrioles markedly enlarged dense bodies accompanied by striated bodies are accumulated, from prophase beyond metaphase. it is concluded that these bodies in untreated cells play a role in the formation of the spindle microtubules. - during metaphase the chromosome are smooth-surfaced and show a pronounced tendency to stickiness. during ana-telophase they become su ...1976986934
[electron microscopy study of pycnotic degeneration in spermatogonia of triturus helveticus razoumowsky]. 19744418646
[incorporation of i-125 iodine in morphologic structures of the ovary in triturus helveticus raz. study by high resolution autoradiography]. 19744282312
[tooth morphology and dental arrangement in neotenic newts (triturus helveticus raz.)]. 19734804700
[decrease in thyroid activity in neotenic triturus helveticus raz. individuals. cytologic and autoradiographic study]. 19724640396
[development of a reticular complex during the evolution of primary spermatogonia in triturus helveticus razoumowsky]. 20045104271
[the formation and role of nucleoli in the oocytes of triturus helveticus raz--an autoradiographic and ultrastructural study]. 19695808093
[nucleocytoplasmic relations in primary spermatogonia of triturus helveticus raz. during their development]. 19694977773
[membranaceous formations in the nucleus and perinuclear cytoplasm of the early ovocyte of triturus helveticus raz]. 19684236005
[effect of butyric acid on the mitotic apparatus of the segmentation mitoses in triturus helveticus raz]. 19685676108
[relationship between the follicle cells and the ovocyte at the beginning of atresia in triturus helveticus ras]. 19684241528
[the rna and protein synthesis in larvae of triturus helveticus in normal condition and during or after temporary oxygen deficiency (histoautoradiographic studies)]. 19675590931
[dna metabolism of the triturus helveticus embryo in late development and its disturbance by temporary respiratory inhibition (based on historadiographic serial studies)]. 19665991197
[the differentiation of the fine structure of triturus helveticus from the ovum to the functional neural cell under normal conditions and its disturbances under temporary oxygen deficiency]. 19666011724
[the dna metabolism of triturus helveticus embryos in early development and its disturbance by temporary respiratory inhibition (after histoautoradiographic studies)]. 19666011586
[the maps of the "lampbrush" chromosomes of triturus (amphibia urodela). ii. triturus helveticus helveticus]. 20155993481
[historadioautographic studies on the dna synthesis in normal larvae of triturus helveticus and following oxygen deficiency]. 19665982943
[the action of vincaleukoblastine on mitosis in triturus helveticus raz]. 196414204906
a study of environmental influences on the size of the pronephros and related structures in triturus helveticus. 195913824245
[antimitotic and teratogenic action of sodium para-aminosalicylate in triturus helveticus raz]. 201813471023
[effect of a localized burn on the egg of triturus helveticus; reaction of the cortex; regulation of the healthy part]. 195713473270
[effects of deacetyl-n-methylcolchicine on mitosis in the segmental egg, the larva and the spermatogenesis of triturus helveticus raz]. 195613414246
[effect of nitrogen mustard on segmentation mitosis, epithelial and germinal cells in triturus helveticus raz]. 195514390865
[the diagnostic value of the light crescent and the possibility of neurulation of the ventral fragment of the egg of triturus helveticus]. 195213020266
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