| lytic myophage abp53 encodes several proteins similar to those encoded by host acinetobacter baumannii and phage phiko2. | acinetobacter baumannii is an important gram-negative opportunistic pathogen causing nosocomial infections. the emergence of multiple drug resistant a. baumannii isolates has increased in recent years. directed towards phage therapy, a lytic phage of a. baumannii, designated abp53, was isolated from a sputum sample in this study. abp53 has an isometric head and a contractile tail with tail fibers, belonging to myoviridae, a latent period of about 10 min, and a burst size of approximately 150 pfu ... | 2011 | 21821767 |
| the pko2 linear plasmid prophage of klebsiella oxytoca. | temperate bacteriophages with plasmid prophages are uncommon in nature, and of these only phages n15 and py54 are known to have a linear plasmid prophage with closed hairpin telomeres. we report here the complete nucleotide sequence of the 51,601-bp klebsiella oxytoca linear plasmid pko2, and we demonstrate experimentally that it is also a prophage. we call this bacteriophage phiko2. an analysis of the 64 predicted phiko2 genes indicate that it is a fairly close relative of phage n15; they share ... | 2004 | 14996813 |
| protelomerase uses a topoisomerase ib/y-recombinase type mechanism to generate dna hairpin ends. | protelomerases are enzymes responsible for the generation of closed hairpin ends in linear dna. it is proposed that they use a breaking-and-rejoin type mechanism to affect dna rearrangement on specific dna sequences. in doing so, one strand turns around and becomes the complementary strand. using the purified enzyme from the escherichia coli phage n15 and the klebsiella phage phiko2 and synthetic oligonucleotide substrates, we directly demonstrate the location where the cutting/re-ligation occur ... | 2004 | 15001353 |