Publications

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complete genome sequence of an isolate of pepper veinal mottle virus and phylogenetic relationship with other potyviruses. 200818998046
virus surveys of capsicum spp. in the republic of benin reveal the prevalence of pepper vein yellows virus and the identification of a previously uncharacterised polerovirus species.surveys were conducted in 2014 and 2015 in southern and northern benin, respectively, to identify the viruses infecting peppers (capsicum spp.). the samples were screened by elisa for cucumber mosaic virus (cmv), pepper veinal mottle virus (pvmv), potato virus y (pvy) and tomato yellow leaf curl virus (tylcv). a generic reverse transcription pcr (rt-pcr) was used to test for the presence of poleroviruses. elisa tests confirmed the prevalence of all viruses, while the rt-pcr detected pepper vein ...201728220324
complete genome analysis of a novel recombinant isolate of pepper veinal mottle virus from mainland china.pepper veinal mottle virus (pvmv) was well established in africa, and also reported infecting pepper (capsicum annuum l) in taiwan and india. however, there is not available of pvmv in mainland china. here, the first complete genome sequence of pvmv isolated from pepper in mainland china was reported.201526573722
occurrence and distribution of pepper veinal mottle virus and cucumber mosaic virus in pepper in ibadan, nigeria.viral diseases constitute obstacles to pepper production in the world. in nigeria, pepper plants are primarily affected by pepper veinal mottle virus (pvmv), cucumber mosaic virus (cmv), pepper leaf curl virus (tlcv), tobacco mosaic virus (tmv), pepper mottle virus (pmv) and a host of other viruses. the experiment was carried out with a diagnostic survey on the experimental field of the national horticultural research institute, ibadan, nigeria and on pepper farms in six local government areas w ...201222495040
determination of the complete nucleotide sequence of a lupine potyvirus isolate from the czech republic reveals that it belongs to a new member of the genus potyvirus.the complete nucleotide sequence of the ssrna genome of a lupine potyvirus (lp) isolate was determined. it comprised 10,113 nucleotides excluding the poly(a) tail. phylogenetic analysis of cp protein sequences identified pepper veinal mottle virus, narcissus yellow stripe virus, and chili veinal mottle virus as the closest relatives, sharing coat protein amino acid sequence identities of only about 64% with the lp isolate. thus, lp can be regarded as a member of a newly described potyvirus speci ...201020981559
allele mining in the pepper gene pool provided new complementation effects between pvr2-eif4e and pvr6-eif(iso)4e alleles for resistance to pepper veinal mottle virus.molecular cloning of recessive resistance genes to potyviruses in a large range of host species identified the eukaryotic translation initiation factor 4e (eif4e) as an essential determinant in the outcome of potyvirus infection. resistance results from a few amino acid changes in the eif4e protein encoded by the recessive resistance allele that disrupt the direct interaction with the potyviral protein vpg. in plants, several loci encode two protein subfamilies, eif4e and eif(iso)4e. while most ...200919641047
serological, molecular, and pathotype diversity of pepper veinal mottle virus and chili veinal mottle virus.abstract variability within the pepper-infecting potyviruses pepper veinal mottle virus (pvmv) and chili veinal mottle virus (chivmv) in africa and asia was investigated. coat protein (cp) gene sequence diversity revealed three clades that corresponded to three geographic locations and there was no evidence of presence of the chivmv/asian group in western or central africa. these clades included closely related isolates that potentially belong to two viral species, which is consistent with curre ...200518943114
design and application of two novel degenerate primer pairs for the detection and complete genomic characterization of potyviruses.two pairs of degenerate primers were designed from sequences within the potyviral ci (cifor/cirev) and hc-pro-coding regions (hpfo/hprev), and these were shown to be highly specific to members of the genus potyvirus. using the cifor and cirev primers, three novel potyviruses infecting crop and weed species from vietnam were detected, namely telosma mosaic virus (telmv) infecting telosma (telosma cordata, asclepiadaceae), peace lily mosaic virus (pelmv) infecting peace lily (spathiphyllum patinii ...200817906831
simultaneous mutations in translation initiation factors eif4e and eif(iso)4e are required to prevent pepper veinal mottle virus infection of pepper.capsicum resistance to pepper veinal mottle virus (pvmv) results from complementation between the pvr2 and pvr6 resistance genes: recessive alleles at these two loci are necessary for resistance, whereas any dominant allele confers susceptibility. in line with previous results showing that pvr2 resistance alleles encode mutated versions of the eukaryotic translation initiation factor 4e (eif4e), the involvement of other members of the eif4e multigenic family in pvmv resistance was investigated. ...200616760413
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