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european origin of bradyrhizobium populations infecting lupins and serradella in soils of western australia and south africa.we applied a multilocus phylogenetic approach to elucidate the origin of serradella and lupin bradyrhizobium strains that persist in soils of western australia and south africa. the selected strains belonged to different randomly amplified polymorphic dna (rapd)-pcr clusters that were distinct from rapd clusters of applied inoculant strains. phylogenetic analyses were performed with nodulation genes (noda, nodz, noll, noei), housekeeping genes (dnak, reca, glnii, atpd), and 16s-23s rrna intergen ...200516269740
diversification of lupine bradyrhizobium strains: evidence from nodulation gene trees.bradyrhizobium strains isolated in europe from genisteae and serradella legumes form a distinct lineage, designated clade ii, on nodulation gene trees. clade ii bradyrhizobia appear to prevail also in the soils of western australia and south africa following probably accidental introduction with seeds of their lupine and serradella hosts. given this potential for dispersal, we investigated bradyrhizobium isolates originating from a range of native new world lupines, based on phylogenetic analyse ...200717400786
cytisus villosus from northeastern algeria is nodulated by genetically diverse bradyrhizobium strains.fifty-one rhizobial strains isolated from root nodules of cytisus villosus growing in northeastern algeria were characterized by genomic and phenotypic analyses. isolates were grouped into sixteen different patterns by pcr-rapd. the phylogenetic status of one representative isolate from each pattern was examined by multilocus sequence analyses of four housekeeping genes (16s rrna, glnii, reca, and atpd) and one symbiotic gene (nodc). analysis of 16s rrna gene sequences showed that all the isolat ...201424756908
revision of the taxonomic status of the species rhizobium lupini and reclassification as bradyrhizobium lupini comb. nov.the species rhizobium lupini was isolated from lupinus nodules and included in the approved lists of bacterial names in 1980. nevertheless, on the basis of the analysis of the type strain of this species available in dsmz, dsm 30140(t), whose 16s rrna gene was identical to that of the type strain of bradyrhizobium japonicum , r. lupini was considered a later synonym of this species. in this study we confirmed that the strain dsm 30140(t) belongs to the species b. japonicum , but also that it can ...201525609676
diversity of bradyrhizobium strains nodulating lupinus micranthus on both sides of the western mediterranean: algeria and spain.lupinus micranthus is a lupine distributed in the mediterranean basin whose nitrogen fixing symbiosis has not been described in detail. in this study, 101 slow-growing nodule isolates were obtained from l. micranthus thriving in soils on both sides of the western mediterranean. the diversity of the isolates, 60 from algeria and 41 from spain, was addressed by multilocus sequence analysis of housekeeping genes (16s rrna, atpd, glnii and reca) and one symbiotic gene (nodc). using genomic fingerpri ...201627236566
lupinus albus plants acquire mercury tolerance when inoculated with an hg-resistant bradyrhizobium strain.one strain of bradyrhizobium canariense (l-7ah) was selected for its metal-resistance and ability to nodulate white lupin (lupinus albus l.) plants, from a collection of rhizobial strains previously created from soils of the almadén mining district (spain) with varying levels of hg contamination. plants were inoculated with either strain l-7ah (hg-tolerant) or l-3 (hg-sensitive, used as control), and watered with nutrient solutions supplemented with various concentrations (0-200 μm) of hgcl2 in ...201324125840
bradyrhizobium canariense sp. nov., an acid-tolerant endosymbiont that nodulates endemic genistoid legumes (papilionoideae: genisteae) from the canary islands, along with bradyrhizobium japonicum bv. genistearum, bradyrhizobium genospecies alpha and bradyrhizobium genospecies beta.highly diverse bradyrhizobium strains nodulate genistoid legumes (brooms) in the canary islands, morocco, spain and the americas. phylogenetic analyses of its, atpd, glnii and reca sequences revealed that these isolates represent at least four distinct evolutionary lineages within the genus, namely bradyrhizobium japonicum and three unnamed genospecies. dna-dna hybridization experiments confirmed that one of the latter represents a new taxonomic species for which the name bradyrhizobium canarien ...200515774626
phylogeny of bradyrhizobia from chinese cowpea miscellany inferred from 16s rrna, atpd, glnii, and 16s-23s intergenic spacer sequences.the cowpea (vigna unguiculata l.), peanut (arachis hypogaea l.), and mung bean (vigna radiata l.) belong to a group of plants known as the "cowpea miscellany" plants, which are widely cultivated throughout the tropic and subtropical zones of africa and asia. however, the phylogeny of the rhizobial strains that nodulate these plants is poorly understood. previous studies have isolated a diversity of rhizobial strains from cowpea miscellany hosts and have suggested that, phylogenetically, they are ...201121491983
mercury-resistant rhizobial bacteria isolated from nodules of leguminous plants growing in high hg-contaminated soils.a survey of symbiotic bacteria from legumes grown in high mercury-contaminated soils (almadén, spain) was performed to produce a collection of rhizobia which could be well adapted to the environmental conditions of this region and be used for restoration practices. nineteen hg-tolerant rhizobia were isolated from nodules of 11 legume species (of the genera medicago, trifolium, vicia, lupinus, phaseolus, and retama) and characterized. based on their growth on hg-supplemented media, the isolates w ...201222246528
survival and competitiveness of bradyrhizobium japonicum strains 20 years after introduction into field locations in poland.it was previously demonstrated that there are no indigenous strains of bradyrhizobium japonicum forming nitrogen-fixing root nodule symbioses with soybean plants in arable field soils in poland. however, bacteria currently classified within this species are present (together with bradyrhizobium canariense) as indigenous populations of strains specific for nodulation of legumes in the genisteae tribe. these rhizobia, infecting legumes such as lupins, are well established in polish soils. the stud ...201526048934
presence of a novel 16s-23s rrna gene intergenic spacer insert in bradyrhizobium canariense strains.seven slow-growing bacterial strains isolated from root nodules of yellow serradella (ornithopus compressus) that originated from asinara island on north western sardinia in italy were characterized by partial 16s rrna gene and intergenic spacer (its) sequencing as well as amplified fragment length polymorphism (aflp) genomic fingerprinting. the results indicated that the o. compressus isolates belong to the bradyrhizobium canariense species. the analysis of its sequences divided the branch of b ...200717241238
characterization of rhizobia from legumes of agronomic interest grown in semi-arid areas of central spain relates genetic differences to soil properties.a study of symbiotic bacteria from traditional agricultural legumes from central spain was performed to create a collection of rhizobia from soils differing in physicochemical, analytical and/or agroecological properties which could be well-adapted to the environmental conditions of this region, and be used for sustainable agricultural practices. thirty-six isolates were obtained from root-nodules of fifteen legume species (including cicer arietinum, lathyrus sativus, lens culinaris, lupinus spp ...201121953333
bradyrhizobium cytisi sp. nov. isolated from effective nodules of cytisus villosus in morocco.several strains isolated from cytisus villosus nodules have been characterized on the basis of diverse genetic, phenotypic and symbiotic approaches. according to their 16s rrna genes they formed a group closely related to bradyrhizobium canariense bta-1(t) with 99.4% identity. the analysis of three housekeeping genes reca, atpd and glnii, suggested that the c. villosus strains represent a new bradyrhizobium species whose closest relative is also b. canariense bta-1(t) with 94.2, 96.7 and 94.5% i ...201121257682
strains nodulating lupinus albus on different continents belong to several new chromosomal and symbiotic lineages within bradyrhizobium.in this work we analysed different chromosomal and symbiotic markers in rhizobial strains nodulating lupinus albus (white lupin) in several continents. collectively the analysis of their rrs and atpd genes, and 16s-23s intergenic spacers (its), showed that they belong to at least four chromosomal lineages within the genus bradyrhizobium. most isolates from the canary islands (near to the african continent) grouped with some strains isolated on mainland spain and were identified as bradyrhizobium ...201020135225
multilocus sequence analysis of the genus bradyrhizobium.the use of multilocus sequence analysis (mlsa) for the taxonomy of bradyrhizobium was assessed. we compared partial sequences for atpd, reca, gyrb, rpob and dnak for a set of reference strains representing named species and genospecies, and a number of new isolates from lupinus albus, arachis hypogaea and ornithopus compressus from spain. the phylogenies of the individual genes were compared with previous dna-dna hybridization results. high hybridization values were well reflected, but intermedi ...200919201125
characterization of bradyrhizobium species isolated from root nodules of cytisus villosus grown in morocco.from a total of 73 bacterial strains isolated from root nodules of cytisus villosus grown in soils of the central-western region of the moroccan rif, 68 strains clustered into 19 repetitive extragenic palindromic (rep)-polymerase chain reaction (pcr) groups. the nearly complete 16s rrna gene sequence from each strain showed they were closely related to members of the genus bradyrhizobium of the alphaproteobacteria, but affiliation at the species level was not clear. sequencing of the housekeepin ...201121757309
perturbation of an arctic soil microbial community by metal nanoparticles.technological advances allowing routine nanoparticle (np) manufacture have enabled their use in electronic equipment, foods, clothing and medical devices. although some nps have antibacterial activity, little is known about their environmental impact and there is no information on the influence of nps on soil in the possibly vulnerable ecosystems of polar regions. the potential toxicity of 0.066% silver, copper or silica nps on a high latitude (>78°n) soil was determined using community level ph ...201121546158
bradyrhizobium canariense and bradyrhizobium japonicum are the two dominant rhizobium species in root nodules of lupin and serradella plants growing in europe.forty three bradyrhizobium strains isolated in poland from root nodules of lupin species (lupinus albus, l. angustifolius and l. luteus), and pink serradella (ornithopus sativus) were examined based on phylogenetic analyses of three housekeeping (atpd, glnii and reca) and nodulation (noda) gene sequences. additionally, seven strains originating from root-nodules of yellow serradella (o. compressus) from asinara island (italy) were included in this study. phylogenetic trees revealed that 15 serra ...201121514760
stress tolerance, genetic analysis and symbiotic properties of root-nodulating bacteria isolated from mediterranean leguminous shrubs in central spain.nine root-nodulating bacterial isolates were obtained from the leguminous shrubs spartium junceum, adenocarpus hispanicus, cytisus purgans, cytisus laburnuum, retama sphaerocarpa and colutea arborescens in areas of central spain. a poliphasic approach analyzing phenotypic, symbiotic and genetic properties was used to study their diversity and characterize them in relation to mediterranean conditions. stress tolerance assays revealed marked variations in salinity, extreme ph and cadmium tolerance ...200918784916
diversity of rhizobia nodulating wild shrubs of sicily and some neighbouring islands.legume shrubs have great potential for rehabilitation of semi-arid degraded soils in mediterranean ecosystems as they establish mutualistic symbiosis with n-fixing rhizobia. eighty-eight symbiotic rhizobia were isolated from seven wild legume shrubs native of sicily (southern italy) and grouped in operational taxonomic units (otu) by analysis of the ribosomal internal transcribed spacer (its) polymorphism. partial sequencing of 16s rrna gene of representative isolates of each otu revealed that m ...200818566799
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