| halocidin: a new antimicrobial peptide from hemocytes of the solitary tunicate, halocynthia aurantium. | from hemocytes of the tunicate halocynthia aurantium we purified a new antimicrobial peptide named halocidin. the native peptide had a mass of 3443 da and comprised two different subunits containing 18 amino acid residues (wlnallhhglncakgvla) and 15 residues (allhhglncakgvla), which were linked covalently by a single cystine disulfide bond. two different monomers were separately synthesized and used to make three additional isoforms (15 residue homodimer, 18 residue homodimer, heterodimer). in a ... | 2002 | 12067731 |
| halomonas halocynthiae sp. nov., isolated from the marine ascidian halocynthia aurantium. | the marine bacterium strain kmm 1376t was isolated from gill tissue of the ascidian halocynthia aurantium, an inhabitant of the coastal waters of the sea of japan. strain kmm 1376t is an aerobic, gram-negative, non-motile, encapsulated, non-pigmented, slightly halophilic bacterium that is characterized by fimbria-like structures, growth in 0.5-15% nacl at 7-35 degrees c and absence of acid production from many carbohydrates. the dna g+c content is 54 mol%. the main fatty acids are c16:0, c16.1om ... | 2002 | 12361285 |
| biological activities of synthetic analogs of halocidin, an antimicrobial peptide from the tunicate halocynthia aurantium. | halocidin is a heterodimer antimicrobial peptide previously isolated from the tunicate halocynthia aurantium. based on the larger monomer (18hc) of halocidin, nine halocidin congeners, including a series of 6 peptides truncated successively from the carboxyl-terminal end of 18hc and 3 analogs (18hckk, k19hc, and k19hckk), which have lysine residues in place of two internal histidines or have a lysine added to the amino terminus of the 18hc molecule, were prepared. each peptide was also converted ... | 2003 | 12878508 |
| characterization of surfactin-like cyclic depsipeptides synthesized by bacillus pumilus from ascidian halocynthia aurantium. | a marine bacterium (kmm 1364), identified as bacillus pumilus, was isolated from the surface of ascidian halocynthia aurantium. structural analysis revealed that the strain kmm 1364 produced a mixture of lipopeptide surfactin analogs with major components with molecular masses of 1035, 1049, 1063, and 1077. the variation in molecular weight represents changes in the number of methylene groups in the lipid and/or peptide portions of the compounds. structurally, these lipopeptides differ from surf ... | 2002 | 14961278 |
| investigations into the ability of the peptide, hal18, to interact with bacterial membranes. | halocidin was isolated from hemocytes, halocynthia aurantium as a heterodimeric peptide consisting of two alpha-helical subunits, hal15 and hal18. hal18 was shown to have antibacterial properties against bacillus subtilis (mlc = 15 microm) and escherichia coli (mlc = 100 microm). the peptide was shown to produce stable monolayers, which were characteristic of alpha-helical peptides predicted to orientate parallel to the surface of the interface. constant area assays showed that hal18 was surface ... | 2008 | 18600320 |
| dicynthaurin: an antimicrobial peptide from hemocytes of the solitary tunicate, halocynthia aurantium. | we isolated a novel antimicrobial peptide, dicynthaurin, from hemocytes of a tunicate, halocynthia aurantium. the native peptide had a mass of approximately 6.2 kda and was composed of two 30-residue monomers without sequence homology to any previously identified peptides (ilqkavldclkaagsslskaaitaiynkit). most cynthaurin molecules were c-terminally amidated and were linked covalently by a single cystine disulfide bond. when performed in membrane-mimetic environments, circular dichroism studies o ... | 2001 | 11479030 |
| description of lutimonas halocynthiae sp. nov., isolated from a golden sea squirt (halocynthia aurantium), reclassification of aestuariicola saemankumensis as lutimonas saemankumensis comb. nov. and emended description of the genus lutimonas. | a gram-stain-negative, non-motile, coccoid, ovoid or rod-shaped bacterial strain, designated rss3-c1(t), was isolated from a golden sea squirt (halocynthia aurantium) collected from the east sea, south korea. strain rss3-c1(t) was found to grow optimally at 20-25 °c, at ph 7.0-8.0 and in the presence of 2.0% (w/v) nacl. phylogenetic trees based on 16s rrna gene sequences revealed that strain rss3-c1(t) clustered with the type strains of lutimonas vermicola and aestuariicola saemankumensis. strai ... | 2014 | 24614845 |
| litoreibacter ascidiaceicola sp. nov., isolated from the golden sea squirt halocynthia aurantium. | a gram-stain-negative, aerobic, non-motile and coccoid, ovoid or rod-shaped bacterial strain, designated rss4-c1(t), was isolated from a golden sea squirt (halocynthia aurantium) collected from the east sea, south korea. the novel strain grew optimally at 25 °c, at ph 7.0-8.0 and in the presence of 1.0-3.0% (w/v) nacl. phylogenetic trees based on 16s rrna gene sequences revealed that strain rss4-c1(t) fell within the clade comprising the type strains of species of the genus litoreibacter . strai ... | 2014 | 24812362 |
| ascidiaceihabitans donghaensis gen. nov., sp. nov., isolated from the golden sea squirt halocynthia aurantium. | a gram-stain-negative, aerobic, non-motile and coccoid, ovoid or rod-shaped bacterial strain, designated rss1-m3(t), was isolated from a golden sea squirt (halocynthia aurantium) collected from the east sea, south korea. strain rss1-m3(t) grew optimally at 30 °c, at ph 7.0-8.0 and in presence of 2.0 % (w/v) nacl. strain rss1-m3(t) exhibited the highest 16s rrna gene sequence similarity (96.55 %) to the type strain of pelagicola litoralis. neighbour-joining and maximum-likelihood phylogenetic tre ... | 2014 | 25205799 |
| molecular systematics of marine gregarine apicomplexans from pacific tunicates, with descriptions of five novel species of lankesteria. | the eugregarines are a group of apicomplexan parasites that mostly infect the intestines of invertebrates. the high level of morphological variation found within and among species of eugregarines makes it difficult to find consistent and reliable traits that unite even closely related lineages. based mostly on traits observed with light microscopy, the majority of described eugregarines from marine invertebrates has been classified into a single group, the lecudinidae. our understanding of the o ... | 2015 | 25985834 |
| thermotropic behavior of major phospholipids from marine invertebrates: changes with warm-acclimation and seasonal acclimatization. | the crystal-liquid crystal-isotropic melt phase transitions of phosphatidylcholine (pc) and phosphatidylethanolamine (pe) from muscle tissue of five species (actinia metridium senile fimbriatum, mussel crenomytilus grayanus, sea-urchin strongylocentrotus intermedius, starfish distolasterias nipon and the ascidian halocynthia aurantium) of marine invertebrates, collected in winter at 0 degrees c and then acclimated to 18.5 degrees c for 5 days, were studied by differential scanning calorimetry an ... | 2002 | 12381376 |
| tenacibaculum ascidiaceicola sp. nov., isolated from the golden sea squirt halocynthia aurantium. | a gram-stain-negative, non-flagellated, non-spore-forming bacterial strain motile by gliding, designated rss1-6t, was isolated from a golden sea squirt halocynthia aurantium and its taxonomic position was investigated by using a polyphasic approach. strain rss1-6t grew optimally at 30-37 °c and in the presence of 1.0-4.0 % (w/v) nacl. phylogenetic trees based on 16s rrna gene sequences showed that strain rss1-6t fell within the clade comprising species of the genus tenacibaculum, clustering with ... | 2016 | 26674528 |
| structure of polysaccharide moiety of pseudomonas xanthomarina kmm 1447(t) lipopolysaccharide. | the structural analysis of a polysaccharide moiety of pseudomonas xanthomarina kmm 1447(t) lipopolysaccharide (lps) was carried out. mild acid degradation of lps resulted in identification of two polysaccharides. the major one was built of β-d-glcpnaca residues amidated with l-ala and gly residues randomly. the minor polysaccharide was composed of branched tetrasaccharide repeating units constituted by two d-galр, d-galpnac and ether of d-glc with (2r,4r)-2,4-dihydroxypentanoic acid lactone (2r, ... | 2016 | 27509200 |
| antifungal activity of synthetic peptide derived from halocidin, antimicrobial peptide from the tunicate, halocynthia aurantium. | halocidin is an antimicrobial peptide isolated from the hemocytes of the tunicate. among the several known synthetic halocidin analogues, di-k19hc has been previously confirmed to have the most profound antibacterial activity against antibiotic-resistant bacteria. this peptide has been considered to be an effective candidate for the development of a new type of antibiotic. in this study, we have assessed the antifungal activity of di-k19hc, against a panel of fungi including several strains of a ... | 2006 | 16469314 |
| monomeric analogues of halocidin. | halocidin is a heterodimeric antimicrobial peptide isolated from a tunicate, halocynthia aurantium. we used the most active of the two monomers, an 18 residue amidated peptide, as lead structure and determined the role of each amino acid with alanine scanning. the results obtained led to the synthesis of a first generation of analogues with antimicrobial activity. the selectivity towards bacterial versus mammalian cells has been explored, as well as the specificity for gram positive (staphylococ ... | 2004 | 15455147 |