| 4-(2'-carboxyphenyl)-4-oxobutyryl coenzyme a ester, an intermediate in vitamin k2 (menaquinone) biosynthesis. | enzyme preparations from mycobacterium phlei, escherichia coli and galium mollugo cell suspension cultures were incubated in the presence of 4-(2'-carboxyphenyl)-4-oxobutyrate (i.e. o-succinylbenzoic acid, osb, 1), atp, coenzyme a and mg2+. the main product isolated from the incubation mixture was 4-(2'-carboxyphenyl)-4-oxobutyryl coenzyme a ester (2) as determined by comparison with synthetic coenzyme a esters. synthetic and enzymically formed 4-(2'-carboxyphenyl)-4-oxobutyryl coenzyme a ester ... | 1987 | 2966501 |
| properties of isochorismate hydroxymutase from flavobacterium k3-15. | isochorismate hydroxymutase (isochorismate synthase, e.c. 5.4.99.6) catalyzes the interconversion of chorismic acid [1] and isochorismic acid [2]. the enzyme was extracted from a flavobacterium k3-15 that overproduces vitamin k2 (i.e., menaquinones) and was purified to homogeneity. the n-terminal amino acid sequence and the mol wt (36,240 +/- 100 daltons) were determined by ms following sds pag electrophoresis. the enzyme was characterized (stability, cofactor requirement, isoelectrical point), ... | 1993 | 8229014 |
| effect of mecoprop drift on some plant species of conservation interest when grown in standardized mixtures in microcosms. | there has been an increasing awareness of potential impacts of herbicide drift on to vegetation in nature reserves and field margin habitats adjacent to treated areas. previous work using single species bioassays has suggested that the effects of a single drift event are confined close to the sprayer (< 10 m). in the present study eight native dicotyledonous species, with and without a perennial grass (lolium perenne), were grown in standardized microcosms in order to study (1) the effects of he ... | 1991 | 15092089 |
| the site of the inhibition of the shikimate pathway by glyphosate: ii. interference of glyphosate with chorismate formation in vivo and in vitro. | in the presence of the nonselective herbicide glyphosate (n-[phosphonomethyl]glycine), buckwheat (fagopyrum esculentum moench) hypocotyls and cultured cells of galium mollugo l. accumulate an organic acid, which was identified as shikimate by mass-spectroscopy of its methyl ester. after growth in 0.5 millimolar glyphosate for 10 days, g. mollugo cells contained shikimate in amounts of up to 10% of their dry weight. synthesis of chorismate-derived anthraquinones in g. mollugo was blocked by glyph ... | 1980 | 16661535 |
| host-plant selectivity of rhizobacteria in a crop/weed model system. | belowground microorganisms are known to influence plants' performance by altering the soil environment. plant pathogens such as cyanide-producing strains of the rhizobacterium pseudomonas may show strong host-plant selectivity. we analyzed interactions between different host plants and pseudomonas strains and tested if these can be linked to the cyanide sensitivity of host plants, the cyanide production of bacterial strains or the plant identity from which strains had been isolated. eight strain ... | 2007 | 17786217 |
| examination of secondary metabolites and antioxidant capacity of anthyllis vulneraria, fuchsia sp., galium mollugo and veronica beccabunga. | anthyllis vulneraria l., fuchsia sp., galium mollugo l., and veronica beccabunga l. were selected to analyse the phenolic content and the antioxidant activity by ferric ion reducing antioxidant power (frap) and trolox equivalent antioxidant capacity (teac) assays. the highest polyphenol, tannin, and flavonoid contents were measured in fuchsia species (7.40 ± 0.8, 5.62 ± 0.7 and 0.72 ± 0.1 g/100 g dry weight), while the lowest values were detected in anthyllis vulneraria (0.68 ± 0.02, 0.17 ± 0.03 ... | 2016 | 28000504 |
| unusual compounds from galium mollugo and their inhibitory activities against ros generation in human fibroblasts. | three unusual dioxatricyclodecenone compounds, mollugoside a, e-mollugoside b and z-mollugoside b and, together with known flavonoids, were isolated from the aerial parts of galium mollugo collected in north-eastern algeria. their structures were elucidated by analysis of spectroscopic data, including 1d and 2d nmr. flavonoids and mollugoside a significantly reduced reactive oxygen species (ros) generation in human fibroblasts. | 2016 | 26344381 |
| kinetic modeling and optimization of maceration and ultrasound-extraction of resinoid from the aerial parts of white lady's bedstraw (galium mollugo l.). | in this paper, extraction of resinoid from the aerial parts of white lady's bedstraw (galium mollugo l.) using an aqueous ethanol solution (50% by volume) was studied at different temperatures in the absence and the presence of ultrasound. this study indicated that ultrasound-assisted extraction was effective for extracting the resinoid and gave better resinoid yields at lower extraction temperature and in much shorter time than the maceration. a phenomenological model was developed for modeling ... | 2013 | 22922037 |
| enzymic synthesis of o-succinylbenzoyl-coa in cell-free extracts of anthraquinone producing galium mollugo l. cell suspension cultures. | o-succinylbenzoic acid (osb) is an intermediate in the biosynthesis of shikimatederived anthraquinones. the cell free activation of o-succinylbenzoic acid in extracts of anthraquinone producing cells of galium mollugo l. is demonstrated for the first time. this activation depends on the presence of atp, coenzyme a and mg(2+). the o-succinylbenzoic acid coenzyme a ester was identified by converting it to 1,4-dihydroxy-2-naphthoic acid by a bacterial enzyme, viz. naphthoatesynthase. it is thus dem ... | 1982 | 24259139 |