| identification and molecular characteristics of mutant alleles at the du281 locus in parthenogenetic progeny of darevskia unisexualis. | | 2017 | 16986429 |
| molecular characterization of allelic variants of (gata)n microsatellite loci in parthenogenetic lizards darevskia unisexualis (lacertidae). | populations of parthenogenetic lizards of the genus darevskia consist of genetically identical animals, and represent a unique model for studying the molecular mechanisms underlying the variability and evolution of hypervariable dna repeats. as unisexual lineages, parthenogenetic lizards are characterized by some level of genetic diversity at microsatellite loci. we cloned and sequenced a number of (gata)n microsatellite loci of darevskia unisexualis. pcr products from these loci were also seque ... | 2007 | 17258407 |
| [thermodynamic characterization of di-, tri-, and tetranucleotide loci in parthenogenetic lizards darevskia unisexualis]. | allelic polymorphism of three microsatellite loci from the genome of parthenogenetic lizard darevskia unisexualis was characterized using analysis of free energy (gibbs energy) of the dna/dna duplex formation within the stepwise mutational model. it was demonstrated that the number of microsatellite cluster monomericic units would change to decrease the mean free energy of the locus. in addition, based on the analysis of nucleotide composition, the gc content of each locus was evaluated, and bel ... | 2009 | 19769305 |
| [characterization of retrotransposon bov-b line reverse transcriptase gene sequences in parthenogenetic lizards darevskia unisexualis and bisexual species d. nairensis and d. valentini]. | cloning and sequencing of the partial reverse transcriptase gene (750 bp) of the bov-b line retrotransposon have been held in parthenogenetic lizards darevskia unisexualis and its assumed parental bisexual species d. nairensis and d.valentini. it was shown that the percentage of transcriptionally active copies of this gene, which does not contain a stop codon, is almost the same in the three species and is about 75%. the intragenomic divergence level of these sequences is low and was found to be ... | 2015 | 26107894 |
| [nucleotide sequences of the microsatellite locus du215 (arm) allelic variants in the parthenospecies darevskia armeniaca (lacertidae)]. | using monolocus pcr analysis with the pairs of primers designed for the du215 locus of darevskia unisexualis, allelic polymorphism at the orthologous locus in the populations of the related parthenospecies d. armeniaca was investigated. it was demonstrated that du215 (arm) locus was polymorphic and in the populations of parthenospecies d. armeniaca (n = 127) represented by at least three allelic variants, differing from each other by the size and composition of microsatellite cluster, and by sin ... | 2007 | 17385314 |
| [molecular and genetic characterization of allelic variants of microsatellite loci in parthenogenetic caucasian rock lizards darevskia armeniaca (lacertidae)]. | using a pair of primers selected for the loci deltau 215, deltau 281, and deltau 323 of darevskia unisexualis monolocus pcr analysis of orthologous loci was carried out in populations of the related parthenogenetic species d. armeniaca and in populations of bisexual parental species d. valentini and d. mixta. it was shown that the studied loci were polymorphic and represented in populations of d. armeniaca by several allelic variants. we cloned and sequenced pcr amplification products of the all ... | 2008 | 19172877 |
| instability of (gata)n microsatellite loci in the parthenogenetic caucasian rock lizard darevskia unisexualis (lacertidae). | mini- and microsatellites, comprising tandemly repeated short nucleotide sequences, are abundant dispersed repetitive elements that are ubiquitous in eukaryotic genomes. in humans and other bisexual species hypervariable mini- and microsatellite loci provide highly informative systems for monitoring of germline and somatic instability. however, little is known about the mechanisms by which these loci mutate in species that lack effective genetic recombination. here, multilocus dna fingerprinting ... | 2004 | 14618391 |
| the parthenogenetic rock lizard lacerta unisexualis: an example of limited genetic polymorphism | protein electrophoresis of lacerta unisexualis from three populations found that 21 of 36 allozyme loci were homozygous, while 14 expressed fixed heterozygotes and one locus was variable. three clones were detected at the locus cat-a. two individuals represent two rare clones while all others form a common clone. our favored explanation is the mutation of a preexisting common clone rather than multiple origins. | 1998 | 9419233 |
| molecular cloning and characteristics of allele variants (gata)n, the microsatellite locus du281 of parthenogenetic caucasian rock lizard (darevskia unisexualis) genome. | | 2017 | 15116568 |
| [variability of gata-microsatellite dna in populations of the parthenogenetic species of lizard lacerta unisexualis darevsky]. | multilocus dna fingerprinting was used to analyze the genome variation of mini- and microsatellite dna regions in parthenogenetic caucasian rock lizard lacerta unisexualis. the dna fingerprints obtained with probe m13 were nearly identical in all populations examined (the average similarity index s = 0.992). the fingerprints obtained with probe (gata)4 varied (s = 0.862). polymorphic fragments were assumed to correspond to allelic variants of genetically unstable gata loci. comparison of the fin ... | 2000 | 10867888 |
| [increased variability of (tcc)n microsatelline loci in populations of the parthenogenetic lizard lacerta unisexualis darevsky]. | in four isolated populations of parthenogenetic caucasian rock lizard lacerta unisexualis, variability of (tcc)n loci was examined using multilocus dna fingerprinting. unexpectedly high variability of (tcc)n microsatellites was found in all four populations. the mean similarity index was 0.825, which is higher than similarity estimates obtained for other mini- and microsatellite loci in l. unisexualis and parthenogenetic species l. dahli and l. armeniaca studied earlier. the high variation level ... | 2000 | 11094767 |
| allozyme variation patterns and multiple hybridization origins: clonal variation among four sibling parthenogenetic caucasian rock lizards. | allozyme electrophoresis of four sibling parthenogenetic caucasian rock lizards darevskia unisexualis, d. uzzelli, d. sapphirina, and d. bendimahiensis found seven clones and five variable loci. the data supported the hypothesis that d. raddei and d. valentini are the parental species of all four parthenogens. variation patterns in darevskia were summarized. species that originated from a single f1 typically consisted of one widespread clone with a few rare clones. species with multiple origins ... | 2000 | 11138937 |
| genetic variation and de novo mutations in the parthenogenetic caucasian rock lizard darevskia unisexualis. | unisexual all-female lizards of the genus darevskia that are well adapted to various habitats are known to reproduce normally by true parthenogenesis. although they consist of unisexual lineages and lack effective genetic recombination, they are characterized by some level of genetic polymorphism. to reveal the mutational contribution to overall genetic variability, the most straightforward and conclusive way is the direct detection of mutation events in pedigree genotyping. earlier we selected ... | 2008 | 18648496 |
| [molecular genetic characteristic of dinucleotide loci in parthenogenetic lizards darevskia unisexualis]. | in the present study, the first molecular genetic investigation of dinucleotide (gt)n microsatellite loci in parthenogenetic lizards darevskia unisexualis was performed. new polymorphic locus, du214, (genebank ac no. eu252542) was identified and characterized in detail. it was demonstrated that allele of this locus differed in the size and structure of microsatellite locus, as well as in point mutations, the combinations of which enabled the isolation of stabile fixed double nucleotide substitut ... | 2009 | 19334618 |
| polymorphism of microsatellite dinucleotide loci in parthenogenetic lizards darevskia unisexualis. | | 2017 | 19341096 |
| [molecular structure of the allelic variants of (aat)n microsatellite locus du47d in the parthenogenetic species darevskia unisexualis and bisexual parental species d. valentini and d. raddei]. | microsatellite repeats are one of the most widespread elements of the eukaryotic genome, but are poorly studied in species with clonal reproduction. pcr analysis and dna sequencing were used to study the molecular structure of the allelic variants of microsatellite locus du47d in the parthenogenetic species darevskia unisexualis and its evolutionary ancestors, bisexual species d. raddei and d. valentini, of the genus darevskia (lacerta saxicola complex). sequencing showed that the allelic varian ... | 2010 | 20583609 |
| reticulate evolution of the rock lizards: meiotic chromosome dynamics and spermatogenesis in diploid and triploid males of the genus darevskia. | knowing whether triploid hybrids resulting from natural hybridization of parthenogenetic and bisexual species are fertile is crucial for understanding the mechanisms of reticulate evolution in rock lizards. here, using males of the bisexual diploid rock lizard speciesdarevskia raddei nairensisanddarevskia valentiniand a triploid hybrid maledarevskia unisexualis × darevskia valentini, we performed karyotyping and comparative immunocytochemistry of chromosome synapsis and investigated the distribu ... | 2017 | 28538689 |
| [superinstability (tct/tcc)n of microsatellite dna in parthenogenetic lizards darevskia unisexualis (family lacertidae)]. | using multilocus dna fingerprinting, we have examined variability of (tct)n microsatellite and m13 minisatellite dna repeats in populations, families, and tissues of caucasian parthenogenetic rock lizards darevskia unisexualis (lacertidae). it has been shown for the first time that population and family dna samples of d. unisexualis (75 samples in total) have individually specific dna fingerprinting patterns of (tct)n fragments. analysis of inheritance of (tct)n microsatellites in 46 first-gener ... | 2003 | 14582385 |
| genetic instability of (gata)n microsatellite dna repeats and somatic mosaicism in the unisexual lizards darevskia unisexualis. | | 2017 | 12741137 |
| origin, clonal diversity, and evolution of the parthenogenetic lizard darevskia unisexualis. | the hybridization of female d. raddei and male d. valentini gave rise to the parthenogenetic caucasian rock lizard darevskia unisexualis. a previously identified genetic polymorphism in the species consisted of one common and two allozyme clones. analysis of microsatellites and single nucleotide polymorphisms (snps) from the three species yields estimates of clonal diversity and tests the hypothesis of a single origin for d. unisexualis. | 2020 | 32393253 |
| [study of allelic polymorphism of (gata)n-containing loci in parthenogenetic lizards darevskia unisexualis (lacertidae)]. | the genesis of mini- and microsatellite loci, which is under extensive study in humans and some other bisexual species, have been virtually overlooked in species with clonal mode of reproduction. earlier, using multilocus dna fingerprinting, we have examined variability of some mini- and microsatellite dna markers in parthenogenetic lizards from the genus darevskia. in particular, mutant (gata)n-restrictive dna fragments were found in darevskia unisexualis. in the present study, we examined intr ... | 2004 | 15575501 |