| natronolimnobius baerhuensis gen. nov., sp. nov. and natronolimnobius innermongolicus sp. nov., novel haloalkaliphilic archaea isolated from soda lakes in inner mongolia, china. | three novel isolates of haloalkaliphilic archaea, strains ihc-005t, ihc-010, and n-1311t, from soda lakes in inner mongolia, china, were characterized to elucidate their taxonomic positions. the three strains were aerobic, gram-negative chemoorganotrophs growing optimally at 37-45 degrees c, ph 9.0-9.5, and 15-20% nacl. cells of strains ihc-005t/ihc-010 were motile rods, while those of strain n-1311t were non-motile pleomorphic flats or cocci. the three strains contained diphytanyl and phytanyl- ... | 2004 | 15841343 |
| halopiger xanaduensis gen. nov., sp. nov., an extremely halophilic archaeon isolated from saline lake shangmatala in inner mongolia, china. | strain sh-6(t) was isolated from the sediment of lake shangmatala, a saline lake in inner mongolia (china). cells were pleomorphic. the organism was neutrophilic and required at least 2.5 m (15 %) nacl, but not mgcl(2), for growth; optimal growth occurred at 4.3 m (25 %) nacl. the g+c content of its dna was 63.1 mol%. 16s rrna gene sequence analysis revealed that strain sh-6(t) is a member of the family halobacteriaceae, but there was a low level of similarity with other members of this family. ... | 2007 | 17625165 |
| phylogenetic relationships within the family halobacteriaceae inferred from rpob' gene and protein sequences. | in order to clarify the current phylogeny of the haloarchaea, particularly the closely related genera that have been difficult to sort out using 16s rrna gene sequences, the dna-dependent rna polymerase subunit b' gene (rpob') was used as a complementary molecular marker. partial sequences of the gene were determined from 16 strains of the family halobacteriaceae. comparisons of phylogenetic trees inferred from the gene and protein sequences as well as from corresponding 16s rrna gene sequences ... | 2007 | 17911299 |
| phylogenetic analysis of the archaeal community in an alkaline-saline soil of the former lake texcoco (mexico). | the soil of the former lake texcoco is an extreme environment localized in the valley of mexico city, mexico. it is highly saline and alkaline, where na+, cl(-), hco3(-) and co3(2-) are the predominant ions, with a ph ranging from 9.8 to 11.7 and electrolytic conductivities in saturation extracts from 22 to 150 ds m(-1). metagenomic dna from the archaeal community was extracted directly from soil and used as template to amplify 16s ribosomal gene by pcr. pcr products were used to construct gene ... | 2008 | 18097633 |
| further refinement of the phylogeny of the halobacteriaceae based on the full-length rna polymerase subunit b' (rpob') gene. | a considerable number of species of the halobacteriaceae possess multiple copies of the 16s rrna gene that exhibit more than 5 % divergence, complicating phylogenetic interpretations. two additional problems have been pointed out: (i) the genera haloterrigena and natrinema show a very close relationship, with some species being shown to overlap in phylogenetic trees reconstructed by the neighbour-joining method, and (ii) alkaliphilic and neutrophilic species of the genus natrialba form definitel ... | 2010 | 19946058 |
| natronoarchaeum mannanilyticum gen. nov., sp. nov., an aerobic, extremely halophilic archaeon isolated from commercial salt. | strain ysm-123(t) was isolated from commercial salt made from japanese seawater in niigata prefecture. optimal nacl and mg(2+) concentrations for growth were 4.0-4.5 m and 5 mm, respectively. the isolate was a mesophilic and slightly alkaliphilic haloarchaeon, whose optimal growth temperature and ph were 37 °c and ph 8.0-9.0. phylogenetic analysis based on 16s rrna gene sequence analysis suggested that strain ysm-123(t) is a member of the phylogenetic group defined by the family halobacteriaceae ... | 2010 | 19965994 |
| temperature and ph optima of extremely halophilic archaea: a mini-review. | archaeal microorganisms that grow optimally at na(+) concentrations of 1.7 m, or the equivalent of 10% (w/v) nacl, and greater are considered to be extreme halophiles. this review encompasses extremely halophilic archaea and their growth characteristics with respect to the correlation between the extent of alkaline ph and elevated temperature optima and the extent of salt tolerance. the focus is on poly-extremophiles, i.e., taxa growing optimally at a na(+) concentration at or above 1.7 m (appro ... | 2011 | 21340748 |