| action of a cysteine proteinase from pupae of the blowfly aldrichina grahami on the oxidized b-chain of bovine insulin. | a cysteine proteinase purified from pupae of the blowfly (a. grahami) was tested for its peptide-bond specificity against the oxidized b-chain of insulin. fifteen peptides were separated on hplc using both gradient and isocratic elution methods. analyses of amino acid content and n-terminal amino acids indicated that these were eleven homogeneous peptides produced by digestion and undigested insulin b-chain. glu13-ala14 and tyr26-thr27 were the major cleavage sites, and asn3-gln4, cys7-gly8, tyr ... | 1985 | 3912099 |
| identification of forensically important fly eggs using a potassium permanganate staining technique. | fly eggs found in corpses can be utilized as entomological evidence in forensic investigations of deaths if the species of fly and the developmental rate at a temperature similar to the death scene are known. the species identification of fly eggs is particularly important, and previously, scanning electron microscope has been used for this purpose. herein, we report a simple technique, using light microscopy, to identify forensically important eggs of chrysomya rufifacies (macquart), chrysomya ... | 2004 | 15006363 |
| detection and isolation of highly pathogenic h5n1 avian influenza a viruses from blow flies collected in the vicinity of an infected poultry farm in kyoto, japan, 2004. | during the outbreak of highly pathogenic avian influenza that occurred in tamba town, kyoto prefecture in 2004, a total of 926 flies were collected from six sites within a radius of 2.3 km from the poultry farm. the h5 influenza a virus genes were detected from the intestinal organs, crop, and gut of the two blow fly species, calliphora nigribarbis and aldrichina grahami, by reverse transcription-polymerase chain reaction for the matrix protein (m) and hemagglutinin (ha) genes. the ha gene encod ... | 2006 | 16896143 |
| identification of necrophagous fly species using issr and scar markers. | necrophagous fly is the most common insect evidence collected during a death investigation. we analyzed the dna polymorphism among the five forensically important fly species, namely, lucilia sericata, aldrichina grahami, chrysomya megacephala, parasarcophaga crassipalpis and musca domestica using inter simple sequence repeat (issr) method. nine issr primers selected from 18 primers could amplify 105 clear and stable bands, of which 95 bands were polymorphic. some primers produced completely dif ... | 2007 | 16949234 |
| cuticular hydrocarbon composition in pupal exuviae for taxonomic differentiation of six necrophagous flies. | gas chromatography-mass spectrometry was used to analyze the cuticular hydrocarbons extracted from the pupal exuviae of six necrophagous flies: aldrichina grahami (aldrich), chrysomya megacephala (f.), lucilia sericata (meigen), achoetandrus rufifacies (macquart), boettcherisca peregrina (robineau-desvoidy), and parasarcophaga crassipalpis (macquart). a discriminant model including the variables of peak 1 (tricosane), peak 7 (9-,11-,13-methyl-pentacosane), peak 21 (11,12-;9,13-dimethyl-hexacosan ... | 2007 | 17547230 |
| sequences of the cytochrome c oxidase subunit i (coi) gene are suitable for species identification of korean calliphorinae flies of forensic importance (diptera: calliphoridae). | calliphorinae fly species are important indicators of the postmortem interval especially during early spring and late fall in korea. although nucleotide sequences of various calliphorinae fly species are available, there has been no research on the cytochrome c oxidase subunit i (coi) nucleotide sequences of korean calliphorinae flies. here, we report the full-length sequences of the coi gene of four calliphorinae fly species collected in korea (five individuals of calliphora vicina, five callip ... | 2009 | 19674236 |
| [sequencing of mitochondrial dna co ii gene in carrion-breeding flies and larvae in zhengzhou]. | using co ii sequences to identify common species of carrion-breeding flies and larvae. | 2010 | 20653135 |
| Mitochondrial DNA and STR analyses for human DNA from maggots crop contents: a forensic entomology case from central-southern China. | Insect larvae and adult insects found on human corpses can provide important forensic evidence however it is useful to be able to prove evidence of association. Without this, it could be claimed that the insect evidence was a contaminant or had been planted on the body. This paper describes how mitochondrial DNA (mtDNA) and STR analysis of the crop contents of larvae of the blowfly Aldrichina grahami collected from separated body parts was used to provide evidence of association. | 2011 | 22041753 |
| utility of multi-gene loci for forensic species diagnosis of blowflies. | contemporary studies in forensic entomology exhaustively evaluate gene sequences because these constitute the fastest and most accurate method of species identification. for this purpose single gene segments, cytochrome oxidase subunit i (coi) in particular, are commonly used. however, the limitation of such sequences in identification, especially of closely related species and populations, demand a multi-gene approach. but this raises the question of which group of genes can best fulfill the id ... | 2011 | 21864153 |
| applicability of partial characterization of cytochrome oxidase i in identification of forensically important flies (diptera) from china and egypt. | precise species identification of every insect sample collected from criminal scenes play an essential role in the accurate estimation of postmortem interval. the morphological similarity poses a great challenge for forensic entomologists. dna-based method can be used as a supplemental means of morphological method. in the present study, we demonstrate the applicability of the 304-bp cytochrome oxidase i gene fragment in molecular identification of forensically important diptera. we analyzed 75 ... | 2013 | 23661266 |
| using the developmental gene bicoid to identify species of forensically important blowflies (diptera: calliphoridae). | identifying species of insects used to estimate postmortem interval (pmi) is a major subject in forensic entomology. because forensic insect specimens are morphologically uniform and are obtained at various developmental stages, dna markers are greatly needed. to develop new autosomal dna markers to identify species, partial genomic sequences of the bicoid (bcd) genes, containing the homeobox and its flanking sequences, from 12 blowfly species (aldrichina grahami, calliphora vicina, calliphora l ... | 2013 | 23586044 |
| age-dependent changes in cuticular hydrocarbons of larvae in aldrichina grahami (aldrich) (diptera: calliphoridae). | necrophagous flies, comprising the first wave of insects present in a cadaver, provide a great potential for more accurate determination of the late postmortem interval (pmi) based on their age. cuticular hydrocarbons (chs) are a promising age indicator in some insect species, especially for the larvae of necrophagous flies. gas chromatography (gc) and gas chromatography-mass spectrometry (gc-ms) were used to characterize the age-dependent, quantitative changes in chs of larval aldrichina graham ... | 2014 | 25087108 |
| fine structure of the eggs of blowflies aldrichina grahami and chrysomya pacifica (diptera: calliphoridae). | we report here the fine structure of the eggs of blowflies aldrichina grahami (aldrich) and chrysomya pacifica kurahashi. for a. grahami, the plastron is wide and extends to almost the entire length of the eggs. the plastron near the micropyle is truncated. the polygonal patterns of chorionic sculpture bear a distinct swollen boundary. regarding c. pacifica, the plastron is narrow and extends to almost the entire length of the eggs. the plastron near the micropyle bifurcates to a y-shape, but th ... | 2004 | 15515972 |
| 3-hydroxykynurenine as o2-. scavenger in the blowfly, aldrichina grahami. | we studied the distribution of o2-.-scavenging activity in 6-day-old larvae of aldrichina grahami. total activity was highest in the muscle. the specific activity per milligram of protein in the malpighian tubules was highest, 10 times the highest elsewhere. most of the o2-. scavenging activity in muscle depended on superoxide dismutase. however, the activity in the malpighian tubules mostly depended on 3-hydroxykynurenine. | 1986 | 3767983 |
| proline synthesis from glutamate in the mitochondria isolated from a blowfly, aldrichina grahami. | proline biosynthesis from glutamate was demonstrated in a cell free system prepared from blowfly abdomen. the biosynthetic activity was found mainly in the mitochondrial fraction. the biosynthesis of proline from glutamate required atp, nadph and mg++ as cofactors. | 1980 | 7418833 |
| the distribution of pyrroline carboxylate reductase and proline oxidase in the larva of the blowfly, aldrichina grahami. | p5c reductase and proline oxidase in the larva of the blowfly, aldrichina grahami, were found to be localized mainly in the fat body mitochondrial matrix and the muscle, respectively. | 1980 | 6893438 |
| changes in activities of carbamoylphosphate synthase and aspartate carbamoyltransferase in the life cycle of the blowfly "aldrichina grahami". | | 1970 | 4097993 |
| occurrence of arginase in the blowfly, aldrichina grahami, and changes in its activity in the life cycle. | | 1968 | 4178033 |
| biosynthesis of fatty acids in the larvae of the blowfly, aldrichina grahami, reared aseptically. | | 1968 | 4178032 |
| the effect of biotin deficiency on the biosynthesis of the fatty acids in a blowfly, aldrichina grahami during metamorphosis under aseptic conditions. | | 1967 | 4168930 |
| [resistance to insecticides of aldrichina grahami. i. insecticide susceptibility in female adults of the field strains in kyoto prefecture]. | | 1966 | 5951265 |