| rabies and rabies virus in wildlife in mainland china, 1990-2013. | the number of wildlife rabies and wildlife-associated human and livestock rabies cases has increased in recent years, particularly in the southeast and northeast regions of mainland china. to better understand wildlife rabies and its role in human and livestock rabies, we reviewed what is known about wildlife rabies from the 1990s to 2013 in mainland china. in addition, the genetic diversity and phylogeny of available wildlife-originated rabies viruses (rabvs) were analyzed. several wildlife spe ... | 2014 | 24911887 |
| early diagnosis of irkut virus infection using magnetic bead-based serum peptide profiling by maldi-tof ms in a mouse model. | early diagnosis is important for the prompt post-exposure prophylaxis of lyssavirus infections. to diagnose irkut virus (irkv) infection during incubation in mice, a novel method using magnetic bead-based serum peptide profiling by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) has been established. for this test, serum peptides were concentrated by adsorption to and elution from the magnetic bead-based weak cation ion exchanger. mass spectrograms obt ... | 2014 | 24670473 |
| analysis of the complete genome of the first irkut virus isolate from china: comparison across the lyssavirus genus. | the genome of irkut virus, isolate irkv-thchina12, the first non-rabies lyssavirus from china (of bat origin), has been completely sequenced. in general, coding and non-coding regions of this viral genome are similar to those of other lyssaviruses. however, alignment of the deduced amino acid sequences of the structural proteins of irkv-thchina12 with those of other lyssavirus representatives revealed significant variability between viral species. the nucleoprotein and matrix protein were found ... | 2013 | 23872529 |
| [rabies in bats]. | rabies is a zoonosis ending fatally in all mammals, including humans. unlike the other mammals, this disease is usually not fatal in bats. rabies is caused by lyssaviruses which are divided into several distinct phylogroups comprising 15 known viruses. it is believed that the original hosts of all lyssaviruses are bats. classical rabies virus (rabv) occurs in bats across americas and represents the major cause of rabies in humans and domestic animals there. european bat lyssavirus type 1 (eblv-1 ... | 2016 | 27450525 |
| experimental infection of big brown bats (eptesicus fuscus) with west caucasian bat virus (wcbv). | big brown bats (eptesicus fuscus), either recently captured individuals or survivors from previous experimental infection with irkut virus (irkv), were inoculated with west caucasian bat virus (wcbv), intramuscularly into the masseter (n=7) or neck (n=8) muscles, or orally (n=6). three bats inoculated into the neck muscles developed rabies and died between days 10 and 18. viral rna was detected in a number of tissues but isolation was successful only from the brain. an oral swab of one of these ... | 2008 | 18634495 |
| experimental infection of big brown bats (eptesicus fuscus) with eurasian bat lyssaviruses aravan, khujand, and irkut virus. | here we describe the results of experimental infections of captive big brown bats (eptesicus fuscus) with three newly isolated bat lyssaviruses from eurasia (aravan, khujand, and irkut viruses). infection of e. fuscus was moderate (total, 55-75%). there was no evidence of transmission to in-contact cage mates. incubation periods for irkut virus infection were significantly shorter (p < 0.05) than for either aravan or khujand virus infections. in turn, quantification of viral rna by taqman pcr su ... | 2006 | 16705370 |
| phylogenetic relationships of irkut and west caucasian bat viruses within the lyssavirus genus and suggested quantitative criteria based on the n gene sequence for lyssavirus genotype definition. | the nucleoprotein (n), phosphoprotein (p) and glycoprotein (g) genes of irkut and west caucasian bat viruses (wcbv) were sequenced and compared with those of other lyssaviruses. n gene nucleotide identities provided unequivocal separation of all lyssavirus genotypes with an identity threshold of 82%. on this basis, irkut virus should be considered as a new genotype with particular relatedness to genotypes 4 and 5 (78.0-78.6% identity for n gene nucleotides and 90.4-92.6% for amino acids). furthe ... | 2005 | 15896400 |
| survey for bat lyssaviruses, thailand. | surveillance for lyssaviruses was conducted among bat populations in 8 provinces in thailand. in 2002 and 2003, a total of 932 bats of 11 species were captured and released after serum collection. lyssavirus infection was determined by conducting virus neutralization assays on bat serum samples. of collected samples, 538 were either hemolysed or insufficient in volume, which left 394 suitable for analysis. these samples included the following: pteropus lylei (n = 335), eonycteris spelaea (n = 45 ... | 2005 | 15752440 |
| evaluation of rabies biologics against irkut virus isolated in china. | an irkut virus (irkv) was recently isolated from a bat in china. the protective ability of rabies biologics available in the chinese market and experimental biologics against the rabies virus (rabv) and irkv were assessed in a hamster model via preexposure prophylaxis (prep) and postexposure prophylaxis (pep) experiments. the results demonstrated that a single dose of rabies vaccine did not induce adequate protection against irkv infection. however, routine prep with three doses of vaccine induc ... | 2013 | 23946522 |
| isolation of irkut virus from a murina leucogaster bat in china. | bats are recognized as a major reservoir of lyssaviruses; however, no bat lyssavirus has been isolated in asia except for aravan and khujand virus in central asia. all chinese lyssavirus isolates in previous reports have been of species rabies virus, mainly from dogs. following at least two recent bat-associated human rabies-like cases in northeast china, we have initiated a study of the prevalence of lyssaviruses in bats in jilin province and their public health implications. a bat lyssavirus h ... | 2013 | 23505588 |
| [construction of a recombinant replication-defective human adenovirus type 5 expressing g protein of irkut virus and the immune test in mouse]. | to develop a safe and effective new generation vaccine for irkv-thchina12 prevention, we constructed a non-replicative recombinant human adenovirus carrying the irkv-thchina12 g gene, named as rad5-irkv-g. the irkv-thchina12 g protein expressed by the recombinant human adenovirus in 293ad cells was detected by western blot and indirect immunofluorescence test. to evaluate the immunogenicity of the recombinant, mice were immunized with rad5-irkv-g by intramuscular (i. m.) or intraperitoneal (i. p ... | 2015 | 26951008 |