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paenibacillus xylanilyticus sp. nov., an airborne xylanolytic bacterium.during a search for xylan-degrading micro-organisms, a sporulating bacterium was recovered from xylan-containing agar plates exposed to air in a research laboratory (salamanca university, spain). the airborne isolate (designated strain xil14t) was identified by 16s rrna gene sequencing as representing a paenibacillus species most closely related to paenibacillus illinoisensis jcm 9907t (99.3 % sequence similarity) and paenibacillus pabuli dsm 3036t (98 % sequence similarity). phenotypic, chemota ...200515653909
paenibacillus panacisoli sp. nov., a xylanolytic bacterium isolated from soil in a ginseng field in south korea.a gram-positive, facultatively anaerobic, motile, spore-forming bacterium, designated gsoil 1411t, was isolated from soil of a ginseng field in pocheon province (south korea) and was characterized using a polyphasic approach. comparative analysis of 16s rrna gene sequences revealed that strain gsoil 1411t belongs to the family paenibacillaceae, with closest sequence similarity to the type strains of paenibacillus xylanilyticus (95.7%), paenibacillus illinoisensis (95.2%) and paenibacillus pabuli ...200617082410
paenibacillus provencensis sp. nov., isolated from human cerebrospinal fluid, and paenibacillus urinalis sp. nov., isolated from human urine.gram-negative, spore-forming rods were isolated from a human urine sample (strain 5402403(t)) and a human cerebrospinal fluid sample (strain 4401170(t)). based on genotypic characteristics, these strains belonged to the genus paenibacillus and were closely related. phylogenetic analysis based on 16s rrna gene sequence comparison showed that they clustered with paenibacillus massiliensis 2301065(t) (95.9 and 94.3 % 16s rrna gene sequence similarity, respectively, for strains 5402403(t) and 440117 ...200818319478
saccharibacillus sacchari gen. nov., sp. nov., isolated from sugar cane.a bacterial strain designated gr21t was isolated from apoplastic fluid of saccharum officinarum (sugar cane). phylogenetic analysis based on 16s rrna gene sequences showed that the isolate forms a separate branch within the family 'paenibacillaceae', with paenibacillus as the closest related genus. within this genus, the closest related species is paenibacillus xylanilyticus, with 93.4 % similarity to the sequence of the type strain. the isolate has gram-variable, facultatively anaerobic, rod-sh ...200818676467
paenibacillus taichungensis sp. nov., from soil in taiwan.among a large collection of taiwanese soil isolates, a novel gram-variable, rod-shaped, motile, endospore-forming bacterial strain, strain v10537(t), was subjected to a polyphasic study including 16s rrna and gyrb gene sequence analysis, dna-dna hybridization experiments, cell wall peptidoglycan type, cellular fatty acid composition analysis and comparative phenotypic characterization. 16s rrna gene sequence analysis indicated that the organism belonged to the genus paenibacillus. strain v10537( ...200818984707
characterization of a cold-active β-glucosidase from paenibacillus xylanilyticus kj-03 capable of hydrolyzing isoflavones daidzin and genistin.paenibacillus xylanilyticus kj-03 isolated from konjac field, showed β-glucosidase activity on tryptic soy agar plate supplemented with 0.1 % esculin and 0.25 % ferric ammonium citrate. a genome library was constructed to obtain the β-glucosidase gene and a recombinant clone, pglc2-3 was selected. the 2,247 bp gene encoding kj-03 β-glucosidase consisted of 749 amino acids. the deduced amino acids of bgla were 61 % homologous with that of the β-glucosidase from bacillus cereus ah1272, which belon ...201324141566
an β-1,4-xylanase with exo-enzyme activity produced by paenibacillus xylanilyticus kj-03 and its cloning and characterization.paenibacillus xylanilyticus kj-03 was isolated from soil samples obtained from a field with amorphophallus konjac plants. a gene encoding xylanase was isolated from kj-03 and cloned using a fosmid library. the xyna gene encodes xylanase; it consists of 1,035 bp and encodes 345 amino acids. the amino acid sequence deduced from the p. xylanilyticus kj-03 xylanase showed 81% and 69% identities with those deduced from the p. polymyxa e681 and paenibacillus sp. hpl-001 xylanases, respectively. the xy ...201323462014
gene cloning of endoglucanase cel5a from cellulose-degrading paenibacillus xylanilyticus kj-03 and purification and characterization of the recombinant enzyme.the bacterial strain paenibacillus xylanilyticus kj-03 was isolated from a sample of soil used for cultivating amorphophallus konjac. the cellulase gene, cel5a was cloned using fosmid library and expressed in escherichia coli bl21 (trxb). the cel5a gene consists of a 1,743 bp open reading frame and encodes 581 amino acids of a protein. cel5a contains n-terminal signal peptide, a catalytic domain of glycosyl hydrolase family 5, and duf291 domain with unknown function. the recombinant cellulase wa ...201222350314
paenibacillus enshidis sp. nov., isolated from the nodules of robinia pseudoacacia l.a gram-positive, motile, endospore-forming, rod-shaped bacterium, designated rp-207(t), was isolated from the nodules of robinia pseudoacacia l. plants planted in enshi district, hubei, pr china. phylogenetic analyses based on the 16s rrna gene sequence showed that the novel strain was affiliated to the genus paenibacillus, with its closest relatives being paenibacillus xylanilyticus xil14(t) (95.6%), paenibacillus peoriae dsm8320(t) (95.3%) and paenibacillus polymyxa dsm 36(t) (95.3%). the dna ...201526063444
diversity of bacillus-like bacterial community in the sediments of the bamenwan mangrove wetland in hainan, china.members of the genus bacillus and related spore-forming genera are ubiquitous. however, bacillus-like species isolated from marine sediments have attracted less interest than their terrestrial relatives. here, we investigated the diversity of bacillus-like bacterial communities in the sediments of the bamenwan mangrove wetland in hainan, china, using culture-dependent and culture-independent methods, and present the first report on this subject. we also discovered some potential novel species fr ...201728177800
cr(vi) sorption by free and immobilised chromate-reducing bacterial cells in pva-alginate matrix: equilibrium isotherms and kinetic studies.chromate-resistant bacterial strain isolated from the soil of tannery was studied for cr(vi) bioaccumulation in free and immobilised cells to evaluate its applicability in chromium removal from aqueous solution. based on the comparative analysis of the 16s rrna gene, and phenotypic and biochemical characterization, this strain was identified as paenibacillus xylanilyticus mr12. mechanism of cr adsorption was also ascertained by chemical modifications of the bacterial biomass followed by fourier ...201323361177
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