| diagnosis of polyomavirus infection in seedcrackers (pyrenestes sp.) and blue bills (spermophaga haematina) using dna in situ hybridization. | avian polyomavirus (apv) infection was diagnosed in a closed research colony of seedcrackers (pyrenestes sp.) and blue-bills (spermophaga haematina) using dna in situ hybridization. the dna probe was a 1-kbp double-stranded pcr-generated probe that recognized a conserved nucleotide sequence within the vp-1 gene. using this technique, apv infection was diagnosed, in 25 of 45 birds based upon examination of formalin-fixed paraffin embedded tissues. birds infected with apv apparently had a higher i ... | 1994 | 18671119 |