| identification of host-specificity determinants in betanodaviruses by using reassortants between striped jack nervous necrosis virus and sevenband grouper nervous necrosis virus. | betanodaviruses, the causal agents of viral nervous necrosis in marine fish, have bipartite positive-sense rnas as genomes. the larger genomic segment, rna1 (3.1 kb), encodes an rna-dependent rna polymerase, and the smaller genomic segment, rna2 (1.4 kb), codes for the coat protein. betanodaviruses have marked host specificity, although the primary structures of the viral rnas and encoded proteins are similar among betanodaviruses. however, no mechanism underlying the host specificity has yet be ... | 2004 | 14722280 |
| histopathological studies on viral nervous necrosis of sevenband grouper, epinephelus septemfasciatus thunberg, at the grow-out stage. | abstract viral nervous necrosis caused by sevenband grouper nervous necrosis virus (sgnnv) has occurred in grow-out stages (0-3 years old) of sevenband grouper, epinephelus septemfasciatus, since the 1980s. in the present study, based on histopathological features of the central nervous system (cns) in naturally diseased fish, pernasal infection experiments using grow-out fish were performed and pernasal infection was established as a putative invasion route of sgnnv. the definite sgnnv-targeted ... | 2004 | 15228608 |
| development of a recombinant protein vaccine based on cell-free protein synthesis for sevenband grouper epinephelus septemfasciatus against viral nervous necrosis. | sevenband grouper, epinephelus septemfasciatus, is becoming an important aquaculture species in korea. however, viral nervous necrosis disease is a large problem causing mass mortality in sevenband grouper aquaculture. recombinant protein vaccines are one of the best methods to reduce these economic losses. however, the cell-based expression method mainly produces inclusion bodies and requires additional procedures. in this study, we expressed a recombinant viral coat protein of sevenband groupe ... | 2015 | 26239013 |
| susceptibility of cultured juveniles of several marine fish to the sevenband grouper nervous necrosis virus. | piscine nodaviruses (betanodaviruses) have been tentatively divided into four genotypes (sjnnv, rgnnv, tpnnv and bfnnv) and it is suggested that host specificity is different among these genotypes. in the present study, a betanodavirus [sevenband grouper nervous necrosis virus (sgnnv)] belonging to the redspotted grouper nervous necrosis virus (rgnnv) genotype, to which most betanodaviruses from warm water fish are identified, was evaluated for its pathogenicity to hatchery-reared juveniles of s ... | 2003 | 12962219 |
| betanodavirus as a novel transneuronal tracer for fish. | in order to obtain a potential new tool to analyze networks of the central nervous system of teleost fishes, we tested a fish-pathogenic betanodavirus, sevenband grouper nervous necrosis virus (sgnnv), as a transneuronal tracer using the freshwater angelfish pterophyllum scalare as a test animal. intravitreous injections of sgnnv into the right eye resulted first in the labelings of neuronal cell bodies in the ganglion cell layers of the retina and then those in the inner and outer nuclear layer ... | 2002 | 12359322 |