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characterization of staphylococcus spp. and micrococcus spp. isolated from iberian ham throughout the ripening process.the iberian dry cured ham is an uncooked meat product highly appreciated because of its characteristic flavour. this product is obtained from highly marbled iberian pig hindlegs after 18-24 months of maturation under natural environmental conditions. the role of micrococcaceae in the development of the aroma characteristics of this products remains unclear. identification of gram-positive, catalase-positive cocci isolated from mannitol salt agar plates showed that staphylococcus xylosus followed ...19947703026
characterization of the most frequently encountered staphylococcus sp. in cats.ninety three staphylococci isolated from clinical specimens from cats were characterized and identified. because the biochemical characteristics of staphylococcus felis were very similar to those of staphylococcus simulans, results were submitted to numerical analysis and dna homology. forty-two isolates (45%) were identified as s. felis, and 4 isolates (4%) as s. simulans. the other species identified, in order of their frequency were, 12 staphylococcus aureus (13%), 9 staphylococcus intermediu ...19948042273
tandem duplication in ermc translational attenuator of the macrolide-lincosamide-streptogramin b resistance plasmid pses6 from staphylococcus equorum.a tandem duplication of 23 bp in the ermc gene translational attenuator of plasmid pses6 from staphylococcus equorum which mediated constitutive resistance to macrolide-lincosamide-streptogramin b antibiotics was identified. this duplication included the ribosome binding site for the ermc gene as well as the first 5 bp of the ermc coding sequence. it was postulated that this sequence duplication affects the possible rna conformations so that the ribosome binding site for ermc synthesis is readil ...19968787908
identification and ribotyping of staphylococcus xylosus and staphylococcus equorum strains isolated from goat milk and cheese.twenty-five strains of staphylococci isolated from goat milk and cheese were identified as belonging to the staphylococcus xylosus/equorum group using the id 32 staph system (biomérieux, marcy-l'etoile, france). this system, however, was not able to discriminate between these two species for 19 of the strains tested. ribotyping was performed on these 25 strains, as well as on three reference strains of each of these two species. hybridization membranes were scanned and analyzed using the taxotro ...19968880319
staphylococcus succinus sp. nov., isolated from dominican amber.two bacterial isolates, designated amg-d1t and amg-d2, were recovered from 25-35-million-year-old dominican amber. amg-d1t and amg-d2 biochemically most closely resemble staphylococcus xylosus; they differ physiologically from other staphylococci. fatty acid analysis and comparisons with extensive databases were unable to show relatedness to any specific taxon. moreover, amg-d1t and amg-d2 contain tuberculostearic acid and meso-diaminopimelic acid, characteristic of the g + c-rich coryneform bac ...19989731292
identification of staphylococcus species by 16s-23s rdna intergenic spacer pcr analysis.to investigate whether 16s-23s rdna (rdna) spacer region length polymorphisms are suitable for the identification of staphylococcus strains, the 16s-23s rdna intergenic spacer region lengths of 221 strains belonging to 31 species were studied by using a pcr-based method. each species presented a specific 16s-23s pattern made of 1-8 fragments ranging from 104-771 bp, with the exception of the species staphylococcus warnei, staphylococcus caprae and staphylococcus piscifermentans, which presented ...19989734063
the macrocyclic peptide antibiotic micrococcin p(1) is secreted by the food-borne bacterium staphylococcus equorum ws 2733 and inhibits listeria monocytogenes on soft cheese.staphylococcus equorum ws 2733 was found to produce a substance exhibiting a bacteriostatic effect on a variety of gram-positive bacteria. the metabolite was purified to homogeneity by ammonium sulfate precipitation and semipreparative reversed-phase high-performance liquid chromatography. electrospray mass spectrometry confirmed the high purity of the compound and revealed a molecular mass of 1,143 da. by two-dimensional nuclear magnetic resonance spectroscopy the substance was identified as mi ...200010831414
molecular techniques open up new vistas for typing of coagulase-negative staphylococci.several methods were used to type 64 clinical isolates of coagulase-negative staphylococci (cns) derived from hospitals in morocco. the clinical isolates originated principally from blood cultures and wound sources. these isolates provided the opportunity to substantially compare the proficiency of developing molecular techniques with conventional phenotypic tests for use in the identification of clinical staphylococci. the following molecular methods were examined: utility ribotyping analysis ( ...200111367554
pyridinyl polythiazole class peptide antibiotic micrococcin p1, secreted by foodborne staphylococcus equorum ws2733, is biosynthesized nonribosomally.recently, foodborne staphylococcus equorum ws2733 was isolated from a french red smear cheese on account of its strong inhibitory activity against gram-positive pathogens such as listeria. the antagonistic substance was identified as macrocyclic peptide antibiotic micrococcin p1, which had previously not been reported for the genus staphylococcus. micrococcin p1, also a potent inhibitor of the malaria parasite plasmodium falciparum, is structurally related to thiostrepton, thiocillins and nosihe ...200111737193
l-methionine degradation potentialities of cheese-ripening microorganisms.volatile sulphur compounds are major flavouring compounds in many traditional fermented foods including cheeses. these compounds are products of the catabolism of l-methionine by cheese-ripening microorganisms. the diversity of l-methionine degradation by such microorganisms, however, remains to be characterized. the objective of this work was to compare the capacities to produce volatile sulphur compounds by five yeasts, geotrichum candidum, yarrowia lipolytica, kluyveromyces lactis, debaryomyc ...200111928962
peptidolytic, esterolytic and amino acid catabolic activities of selected bacterial strains from the surface of smear cheese.enzymes produced by bacteria present on the surface of smear cheeses play essential roles in flavour development during cheese ripening. in this study, strains including brevibacteria, corynebacteria, staphylococci and brachybacteria, from the surface of two smear cheese (tilsit and gubeen) were screened for a range of enzyme activities including aminopeptidase (substrates: leu-pna and his-pna), dipeptidase (met-ala, ala-met, pro-ala, his-leu and pro-leu), tripeptidase (phe-gly-gly, gly-gly-gly ...200212051480
staphylococcus equorum subsp. linens, subsp. nov., a starter culture component for surface ripened semi-hard cheeses.two staphylococcal strains, rp29t and rp33, were isolated from the main microflora of a surface ripened swiss mountain cheese made from raw milk. these two strains were differentiated from the most closely related species staphylococcus equorum on the basis of dna-dna hybridisation and phenotypic characteristics and are proposed as staphylococcus equorum subsp. linens subsp. nov. they could be distinguished phenotypically from s. equorum by their sensitivity to all 14 tested antibiotics, especia ...200312747407
bacterial community structure and location in stilton cheese.the microbial diversity occurring in stilton cheese was evaluated by 16s ribosomal dna analysis with pcr-denaturing gradient gel electrophoresis. dna templates for pcr experiments were directly extracted from the cheese as well as bulk cells harvested from a variety of viable-count media. the variable v3 and v4-v5 regions of the 16s genes were analyzed. closest relatives of lactococcus lactis, enterococcus faecalis, lactobacillus plantarum, lactobacillus curvatus, leuconostoc mesenteroides, stap ...200312788761
bacteriology and somatic cell counts in milk samples from ewes on a scottish farm.milk samples from 50 sheep on a single scottish research farm were collected weekly for 10 wk postpartum. samples were analyzed for somatic cell counts (scc) each week and bacteriologic culture was done for 7 of the 10 wk. a total of 492 udder half samples were cultured, of which 467 had corresponding cell count data. statistical analysis on complete scc and culture data showed no association between scc and bacterial isolation, even when more than 10 colonies of a single bacterial species were ...200415352543
rapid and reliable identification of staphylococcus equorum by a species-specific pcr assay targeting the soda gene.rapid and reliable identification of staphylococcus (s.) equorum was achieved by species-specific pcr assays. a set of primers targeting the manganese-dependent superoxide dismutase (soda) gene of s. equorum was designed. species-specificity of the primer set was evaluated by using a total of 112 strains (including 27 reference strains of the dsm collection), representing 26 different species of the genus staphylococcus, 3 species of the genus kocuria, and different strains of macrococcus caseol ...200415612627
design and application of a staphylococcus-specific single strand conformation polymorphism-pcr analysis to monitor staphylococcus populations diversity and dynamics during production of raw milk cheese.development of a nested-pcr single strand conformation polymorphism (sscp) assay targeting the 16s rrna genes of the staphylococcus genus, to monitor staphylococci in cheese.200516033516
surface microflora of four smear-ripened cheeses.the microbial composition of smear-ripened cheeses is not very clear. a total of 194 bacterial isolates and 187 yeast isolates from the surfaces of four irish farmhouse smear-ripened cheeses were identified at the midpoint of ripening using pulsed-field gel electrophoresis (pfge), repetitive sequence-based pcr, and 16s rrna gene sequencing for identifying and typing the bacteria and fourier transform infrared spectroscopy and mitochondrial dna restriction fragment length polymorphism (mtdna rflp ...200516269673
staphylococcus equorum and staphylococcus succinus isolated from human clinical specimens.a polyphasic identification approach was applied to a group of 11 novobiocin-resistant staphylococci isolated from human clinical materials. phenotypic characteristics obtained by both commercial and conventional tests assigned eight strains as staphylococcus xylosus and three strains as ambiguous s. xylosus/staphylococcus equorum. in contrast to biotyping, ribotyping with ecori and hindiii restriction endonucleases and whole-cell protein fingerprinting assigned six analysed strains as s. equoru ...200616585638
rapid analysis of two food-borne microbial communities at the species level by fourier-transform infrared microspectroscopy.the species composition of microbial communities in natural habitats may be extremely complex and therefore a quantitative analysis of the fraction each species contributes to the consortium has proven to be difficult. during recent years, the identification of bacterial pure cultures based on their infrared spectra has been established. fourier-transform infrared microspectroscopy now proceeds a step further and allows identification of microorganisms directly plated from community dilutions. i ...200616623742
invasive potential of bacterial isolates associated with subclinical bovine mastitis.this work describes differences in the invasive ability of bacterial isolates associated with mastitis. invasion ability was determined by the uptake and survival in a primary culture of bovine mammary epithelial cells (bmec). bmec were isolated from a healthy lactating cow and characterized by their morphology, immunostaining for cytokeratin and the detection of beta- and kappa-casein mrnas. ten bacterial isolates comprising the staphylococcal species staphylococcus aureus (3), staphylococcus e ...200616624358
monitoring of staphylococcal starters in two french processing plants manufacturing dry fermented sausages.the growth and survival of staphylococcus xylosus and staphylococcus carnosus were monitored during sausage manufacture in two processing plants.200717184340
effect of industrial and natural biocides on spoilage, pathogenic and technological strains grown in biofilm.this study aimed at investigating bactericide solutions effective on spoilage and pathogenic bacteria while preserving technological bacteria. two compounds of essential oil (thymol and eugenol), one essential oil of satureja thymbra and two industrial biocides (pe 270-30, brillo) were tested on technological strains (staphylococcus equorum, staphylococcus succinus and lactobacillus sakei) grown in monoculture biofilm and on a mixed biofilm of pathogenic bacteria (staphylococcus aureus, listeria ...200717188206
use of an insect cell culture growth medium to isolate bacteria from horses with effusive, fibrinous pericarditis: a preliminary study.effusive, fibrinous pericarditis is an uncommon disease entity in horses. in 2001, pericarditis occurred in conjunction with an epizootic in central kentucky that was associated with exposure to eastern tent caterpillars (etcs). bacterial isolation from equine pericardial fluid samples was attempted using an insect cell culture growth medium (iccgm). using previously cultured, stored frozen samples from four horses with fibrinous pericarditis, inoculation of 10% blood agar plates yielded no grow ...200717204376
stability of the biodiversity of the surface consortia of gubbeen, a red-smear cheese.a total of 1,052 bacteria and 828 yeasts were isolated from the surface flora of 6 batches of gubbeen cheese made in 1996-1997 and 2002-2003. stability of the microflora was evaluated over time and also during ripening at 4, 10, and 16 d (batches 4, 5, and 6) or at 4, 16, 23, and 37 d (batches 1, 2, and 3). bacteria were identified using pulsed-field gel electrophoresis, repetitive extragenic palindromic-pcr, and 16s rrna gene sequencing, and yeasts were identified by fourier transform infrared ...200717430918
stability of microbial communities in goat milk during a lactation year: molecular approaches.the microbial communities in milks from one herd were evaluated during 1-year of lactation, using molecular methods to evaluate their stability and the effect of breeding conditions on their composition. the diversity of microbial communities was measured using two approaches: molecular identification by 16s and 18s rdna sequencing of isolates from counting media (two milks), and direct identification using 16s rdna from clone libraries (six milks). the stability of these communities was evaluat ...200717604934
production of volatile aroma compounds by bacterial strains isolated from different surface-ripened french cheeses.twelve bacterial strains belonging to eight taxonomic groups: brevibacterium linens, microbacterium foliorum, arthrobacter arilaitensis, staphylococcus cohnii, staphylococcus equorum, brachybacterium sp., proteus vulgaris and psychrobacter sp., isolated from different surface-ripened french cheeses, were investigated for their abilities to generate volatile aroma compounds. out of 104 volatile compounds, 54 volatile compounds (identified using dynamic headspace technique coupled with gas chromat ...200717701035
safety improvement and preservation of typical sensory qualities of traditional dry fermented sausages using autochthonous starter cultures.traditional dry fermented sausages are manufactured without addition of starter cultures in small-scale processing units, their fermentation relying on indigenous microflora. characterisation and control of these specific bacteria are essential for the sensory quality and the safety of the sausages. the aim of this study was to develop an autochthonous starter culture that improves safety while preserving the typical sensory characteristics of traditional sausages. an autochthonous starter compo ...200818573561
antibiotic resistance of coagulase-negative staphylococci associated with food and used in starter cultures.the resistance of 330 coagulase-negative staphylococci (cns) associated with food or used in starter cultures and belonging to the species staphylococcus carnosus, staphylococcus condimenti, staphylococcus piscifermentans, staphylococcus equorum, staphylococcus succinus and staphylococcus xylosus, against 21 antibiotics was determined using the disk diffusion method. the incidence and number of resistances was found to be species and source of isolation dependent. most strains of s. equorum (63% ...200818625535
characterization of toxin production of coagulase-negative staphylococci isolated from food and starter cultures.in this study a comprehensive analysis of toxin production of food associated coagulase-negative staphylococci (cns) was investigated. the strains belong to the following staphylococcal species, staphylococcus carnosus, staphylococcus condimenti, staphylococcus equorum, staphylococcus piscifermentans, staphylococcus succinus, and staphylococcus xylosus, which were isolated from fermented food and starter cultures. a collection of 330 strains were analyzed with respect to their hemolytic activity ...200818752861
performance of api staph id 32 and staph-zym for identification of coagulase-negative staphylococci isolated from bovine milk samples.in this study, the accuracy of two phenotypic tests, api staph id 32 and staph-zym, was determined for identification of coagulase-negative staphylococci (cns) from bovine milk samples in comparison with identification based on dna-sequencing. a total of 172 cns isolated from bovine milk were classified into 17 species. the most frequently isolated species based on rpob sequencing were staphylococcus chromogenes and staphylococcus epidermidis, followed by staphylococcus xylosus, staphylococcus w ...200919110383
genetic diversity and biofilm formation of staphylococcus equorum isolated from naturally fermented sausages and their manufacturing environment.s. equorum is often isolated from naturally fermented sausages and from the environment of processing units. the aim of this work was first to characterize the genetic diversity of this species in a single small processing unit manufacturing traditional sausages without the use of starter cultures. one hundred and eighteen s. equorum isolates were collected from meat products and surfaces of this unit. secondly, the capacity to form biofilm of 57 isolates of s. equorum selected from pulsed-field ...200919157614
biological control of grey mould in strawberry fruits by halophilic bacteria.grey mould caused by botrytis cinerea is an economically important disease of strawberries in tunisia and worldwide. the aim of this study was to select effective halophilic bacteria from hypersaline ecosystems and evaluate the abilities of antifungal bacteria to secrete extracellular hydrolytic enzymes, anti-botrytis metabolites and volatiles.200919191973
biodiversity of coagulase-negative staphylococci in french cheeses, dry fermented sausages, processing environments and clinical samples.in this study, the biodiversity of coagulase-negative staphylococci (cns) strains isolated in france from cheese related samples (227 isolates) and dry sausage related samples (204 isolates) was compared to the biodiversity of 297 clinical isolates. species identification was performed using different molecular methods (specific pcr, "staph array" hybridization and soda gene sequencing). infraspecific biodiversity of strains belonging to the main cns species found in both food and clinical sampl ...201020061042
biodiversity of indigenous staphylococci of naturally fermented dry sausages and manufacturing environments of small-scale processing units.the staphylococcal community of the environments of nine french small-scale processing units and their naturally fermented meat products was identified by analyzing 676 isolates. fifteen species were accurately identified using validated molecular methods. the three prevalent species were staphylococcus equorum (58.4%), staphylococcus saprophyticus (15.7%) and staphylococcus xylosus (9.3%). s. equorum was isolated in all the processing units in similar proportion in meat and environmental sample ...201020141949
population dynamics of two antilisterial cheese surface consortia revealed by temporal temperature gradient gel electrophoresis.surface contamination of smear cheese by listeria spp. is of major concern for the industry. complex smear ecosystems have been shown to harbor antilisterial potential but the microorganisms and mechanisms involved in the inhibition mostly remain unclear, and are likely related to complex interactions than to production of single antimicrobial compounds. bacterial biodiversity and population dynamics of complex smear ecosystems exhibiting antilisterial properties in situ were investigated by tem ...201020222967
comparative bioremediation potential of four rhizospheric microbial species against lindane.four microbial species (kocuria rhizophila, microbacterium resistens, staphylococcus equorum and staphylococcus cohnii subspecies urealyticus) were isolated from the rhizospheric zone of selected plants growing in a lindane contaminated environment and acclimatized in lindane spiked media (5-100 μg ml⁻¹). the isolated species were inoculated with soil containing 5, 50 and 100 mg kg⁻¹ of lindane and incubated at room temperature. soil samples were collected periodically to evaluate the microbial ...201021044795
ftir-based polyphasic identification of lactic acid bacteria isolated from traditional greek graviera cheese.this study used a combination of phenotypic, physical (fourier transformed infra-red [ftir] spectroscopy) and molecular (rflp and sscp analysis of 16s rrna genes) methods to identify the lactic acid bacteria (lab) flora present in traditional greek graviera cheese after five weeks of ripening. a total of 300 isolates collected from high dilution plates of tsaye (incubated at 30 °c), m-17 (22 °c) and m-17 (42 °c) agar media were clustered by ftir and then representative strains of each cluster we ...201021056778
identification of staphylococcus spp. using (gtg)₅-pcr fingerprinting.a group of 212 type and reference strains deposited in the czech collection of microorganisms (brno, czech republic) and covering 41 staphylococcus species comprising 21 subspecies was characterised using rep-pcr fingerprinting with the (gtg)₅ primer in order to evaluate this method for identification of staphylococci. all strains were typeable using the (gtg)₅ primer and generated pcr products ranging from 200 to 4500 bp. numerical analysis of the obtained fingerprints revealed (sub)species-spe ...201021095086
binding to extracellular matrix proteins and formation of biogenic amines by food-associated coagulase-negative staphylococci.in connection with a study on the dna microarray based detection of genes involved in safety and technologically relevant properties (seitter (née resch) et al., 2011), food-associated coagulase-negative staphylococci (cns) were investigated phenotypically with regard to their ability to bind to the extracellular matrix proteins (ecm) and to produce biogenic amines. the properties have been shown to be involved in the colonization of injured tissue and invasion into host cells as well as in phar ...201121333369
biodiversity and characterization of staphylococcus species isolated from a small manufacturing dairy plant in portugal.the level and the diversity of the staphylococcal community occurring in the environment and dairy products of a small manufacturing dairy plant were investigated. species identification was performed using different molecular methods, viz. multiplex-pcr, amplified ribosomal dna restriction analysis (ardra), and soda gene sequencing. the main species encountered corresponded to staphylococcus equorum (41 isolates, 39.0%), s. saprophyticus (28 isolates, 26.7%) and s. epidermidis (15 isolates, 14. ...201121411163
use of reca gene sequence analysis for the identification of staphylococcus equorum strains predominant on dry-cured hams.spanish dry-cured ham is an uncooked meat product highly appreciated due to its characteristics flavour. in this study, we examined the accuracy of biochemical tests and 16s rdna sequencing in the identification of 56 staphylococcal strains isolated during industrial spanish dry-cured ham processes. important differences were observed comparing genotypic and phenotypic data. staphylococcus xylosus was the prevalent species identified by biochemical methods (87.5%), however, sequencing of the 16s ...201121645821
identification of staphylococci and dominant lactic acid bacteria in spontaneously fermented swiss meat products using pcr-rflp.pathogenic, spoilage, and technologically important microorganisms were monitored in 21 spontaneously fermented swiss meat products manufactured with meat from wildlife or animals grown in natural habitat. thereby, pcr-restriction fragment length polymorphism (rflp) on rpob and 16s rrna gene sequences provided a powerful tool for fast and accurate identification of the main microbial population. lactobacillus sakei and lactobacillus curvatus dominated in fermented meat products followed by staph ...201222202869
catalase-positive cocci in fermented sausage: variability due to different pork breeds, breeding systems and sausage production technology.the aim of this study was to compare the ecology of catalase-positive cocci (cpc) present in traditional fermented sausages produced using different breeds of pork, each of which was raised in two different environments and processed using two different technologies. semi-quantitative molecular methods were used to determine bacterial identities. almost all fermentations were characterised by a significant increase in cpc during the first few days of fermentation, reaching values of 10(5)-10(6)  ...201222202871
biodiversity and characterization of indigenous coagulase-negative staphylococci isolated from raw milk and cheese of north italy.the aim of the current study was to detect coagulase-negative staphylococci (cns) in raw milk and cheeses produced in north italy, and to analyze isolates for their biodiversity, safety aspects and technological properties. molecular identification methods revealed a high biodiversity among isolates and assigned them to 17 species. the most recovered species were staphylococcus equorum (12%), staphylococcus lentus (12%), staphylococcus simulans (12%), staphylococcus sciuri (10%), and staphylococ ...201223498185
characterization of coagulase-negative staphylococci isolated from spanish dry cured meat products.technological and safety-related properties were analyzed in a coagulase-negative staphylococci (cns) collection isolated from spanish dry-cured meat products in order to use them as starter cultures. the highest nitrate reductase and proteolytic activity was showed by staphylococcus carnosus and staphylococcus equorum. only a few strains were able to form biofilms and the presence of the ica gene was analyzed on them. in relation to antibiotic resistance, all s. carnosus and most of the s. equo ...201223273441
characterization of a mazef toxin-antitoxin homologue from staphylococcus equorum.toxin-antitoxin (ta) systems encoded in prokaryotic genomes fall into five types, typically composed of two distinct small molecules, an endotoxic protein and a cis-encoded antitoxin of ribonucleic or proteinaceous nature. in silico analysis revealed seven putative type i and three putative type ii ta systems in the genome of the nonpathogenic species strain staphylococcus equorum se3. among these, a mazef system orthologue termed mazef(seq) was further characterized. 5' rapid amplification of c ...201323104807
microbiota of high-pressure-processed serrano ham investigated by culture-dependent and culture-independent methods.the microbiota of serrano dry-cured ham of different chemical composition, subjected or not to high-pressure processing (hpp), was investigated using culture-dependent and culture-independent methods. microbial counts were submitted to analysis of variance with physicochemical parameters (aw, nacl concentration, salt-in-lean ratio and intramuscular fat content) or hpp as main effects. in untreated hams, physicochemical parameters significantly affected counts of aerobic mesophiles, psychrotrophs ...201727835773
quaternary ammonium biocides as antimicrobial agents protecting historical wood and brick.quaternary ammonium compounds (qacs) are widely used in disinfection of water, surfaces and instruments as well as in textile, leather and food industries because of their relatively low toxicity, broad antimicrobial spectrum, non-volatility and chemical stability. due to these advantages, qacs are also used in restoration and can be applied on historical material. the aim of this study was to determine the usefulness of biocides based on quaternary ammonium salts and containing various excipien ...201626629794
[endophytic bacterial community analysis of catharanthus roseus and its association with huanglongbing pathogen].to analyze the microbial diversity in healthy and hlb-affected catharanthus roseus under manual-grafting conditions and to find the association between the endophytic bacteria and the hlb pathogen.201222799214
microbiota characterization of a belgian protected designation of origin cheese, herve cheese, using metagenomic analysis.herve cheese is a belgian soft cheese with a washed rind, and is made from raw or pasteurized milk. the specific microbiota of this cheese has never previously been fully explored and the use of raw or pasteurized milk in addition to starters is assumed to affect the microbiota of the rind and the heart. the aim of the study was to analyze the bacterial microbiota of herve cheese using classical microbiology and a metagenomic approach based on 16s ribosomal dna pyrosequencing. using classical mi ...201425064656
reduced growth of listeria monocytogenes in two model cheese microcosms is not associated with individual microbial strains.two model antilisterial microbial communities consisting of two yeasts, two gram positive and two gram negative bacteria, and originating from livarot cheese smear were previously designed. they were used in the present study to analyse the impact of microbial population dynamics on growth of listeria monocytogenes in cheese microcosm. specific culture media and pcr primers were developed for simultaneous culture-dependent and real-time pcr quantification of strains belonging to marinomonas sp., ...201323122498
selection of bioprotective cultures for preventing cold-smoked salmon spoilage.biopreservation is a natural technology of food preservation, which consists of inoculating food with microorganisms selected for their antibacterial properties. the objective of this study was to select lactic acid bacteria (lab) to improve the quality of cold-smoked salmon (css). in this work, different strains representative of the 4 dominant species, identified in a previous study by pyrosequencing the 16s rrna gene, were isolated and their spoiling potential in css blocks, sterilized by ion ...201526044337
characterization by culture-dependent and culture-independent methods of the bacterial population of suckling-lamb packaged in different atmospheres.this study offers insight into the dynamics of bacterial populations in fresh cuts of suckling lamb under four different atmospheric conditions: air (a), and three modified atmosphere packaging (map) environments, 15%o2/30%co2/55%n2 (c, commercial), 70%o2/30%co2 (o), and 15%o2/85%co2 (h) for 18 days. microbial analyses by both conventional methods and pcr-dgge were performed. controversial and surprising results emerged from comparing both methods in relation to the genus pseudomonas. thus, conv ...201324010600
carnobacterium divergens - a dominating bacterium of pork meat juice.nonspoiled food that nevertheless contains bacterial pathogens constitutes a much more serious health problem than spoiled food, as the consumer is not warned beforehand. however, data on the diversity of bacterial species in meat juice are rare. to study the bacterial load of fresh pork from ten different distributors, we applied a combination of the conventional culture-based and molecular methods for detecting and quantifying the microbial spectrum of fresh pork meat juice samples. altogether ...201222537055
survival of cheese-ripening microorganisms in a dynamic simulator of the gastrointestinal tract.a mixture of nine microorganisms (six bacteria and three yeasts) from the microflora of surface-ripened cheeses were subjected to in vitro digestive stress in a three-compartment "dynamic gastrointestinal digester" (didgi). we studied the microorganisms (i) grown separately in culture medium only (ii) grown separately in culture medium and then mixed, (iii) grown separately in culture medium and then included in a rennet gel and (iv) grown together in smear-ripened cheese. the yeasts geotrichum ...201626611167
isolation and characterization of three benzylisoquinoline alkaloids from thalictrum minus l. and their antibacterial activity against bovine mastitis.the roots of thalictrum minus are traditionally used in the treatment of inflammation and infectious diseases such as bovine mastitis. however, there are no reports available in literature till date regarding the antibacterial studies of t. minus against bovine mastitis.201627426505
characterization of mobile staphylococcus equorum plasmids isolated from fermented seafood that confer lincomycin resistance.the complete nucleotide sequences of lincomycin-resistance gene (lnua)-containing plasmids in staphylococcus equorum strains isolated from the high-salt-fermented seafood jeotgal were determined. these plasmids, designated pselnu1-3, are 2638-bp long, have two polymorphic sites, and encode typical elements found in plasmids that replicate via a rolling-circle mechanism including the replication protein gene (rep), a double-stranded origin of replication, a single-stranded origin of replication, ...201526448648
unique characteristics of recombinant hybrid manganese superoxide dismutase from staphylococcus equorum and s. saprophyticus.a recombinant hybrid of manganese dependent-superoxide dismutase of staphylococcus equorum and s. saprophyticus has successfully been overexpressed in escherichia coli bl21(de3), purified, and characterized. the recombinant enzyme suffered from degradation and aggregation upon storage at -20 °c, but not at room temperature nor in cold. chromatographic analysis in a size exclusion column suggested the occurrence of dimeric form, which has been reported to contribute in maintaining the stability o ...201626960678
two paralogous yefm-yoeb loci from staphylococcus equorum encode functional toxin-antitoxin systems.toxin-antitoxin (ta) systems are small genetic elements of prokaryotes associated with persister cell formation, phage defence, stress regulation and programmed cell arrest. in this study, we characterized two paralogues of the ribosome-dependent rnase yefm-yoeb ta system from the gram-positive organism staphylococcus equorum se3. 5' rapid amplification of cdna ends confirmed transcriptional activity in the exponential growth phase and revealed an extended 5' untranslated region upstream of the ...201323832005
bacterial ecology of pdo coppa and pancetta piacentina at the end of ripening and after map storage of sliced product.the objective of this study was to evaluate the microbiota of two typical italian pdo delicatessens coppa and pancetta piacentina, produced in piacenza area (italy). classical and molecular approaches were employed, in order to acquire knowledge on their bacterial ecology and its evolution after slicing and map storing; thus, the biodiversity of characteristic bacterial community, already present or introduced during such procedures, was studied in both full ripened and sliced samples from two p ...201424361828
process-driven bacterial community dynamics are key to cured meat colour formation by coagulase-negative staphylococci via nitrate reductase or nitric oxide synthase activities.the cured colour of european raw fermented meats is usually achieved by nitrate-into-nitrite reduction by coagulase-negative staphylococci (cns), subsequently generating nitric oxide to form the relatively stable nitrosomyoglobin pigment. the present study aimed at comparing this classical curing procedure, based on nitrate reductase activity, with a potential alternative colour formation mechanism, based on nitric oxide synthase (nos) activity, under different acidification profiles. to this en ...201525805616
community dynamics of coagulase-negative staphylococci during spontaneous artisan-type meat fermentations differ between smoking and moulding treatments.coagulase-negative staphylococci (cns) that are naturally present in the raw meat batter of fermented sausages or that originate from the addition of a starter culture play a role in flavour development. a wide species diversity of cns can be present in fermented meats, but it is not fully clear yet how specific process parameters select for specific cns by affecting their community dynamics. therefore, the influence of smoking and moulding treatments on the cns community dynamics in spontaneous ...201323880244
use of molecular methods to characterize the bacterial community and to monitor different native starter cultures throughout the ripening of galician chorizo.the development of lactobacillus and staphylococcus strains used as starter cultures throughout the ripening of galician chorizo, a traditional dry fermented sausage from the north-west of spain, was monitored combining different molecular-based techniques. the bacterial diversity occurring in the inoculated sausages at the beginning and the end of the ripening was also studied and compared to the indigenous population in an uninoculated control batch. real-time pcr was used to monitor the lacto ...201323498201
monitoring the bacterial population dynamics during the ripening of galician chorizo, a traditional dry fermented spanish sausage.the dynamics of the bacterial population throughout the ripening of galician chorizo, a traditional dry fermented sausage produced in the north-west of spain, were investigated by using classical and molecular approaches. fermented sausages are certainly complex matrices with pcr inhibitors and background microbiota. therefore, two different dna preparation methods were performed to elaborate each standard curve and no significant differences were found, showing a very good correlation between b ...201323122504
microbial background flora in small-scale cheese production facilities does not inhibit growth and surface attachment of listeria monocytogenes.the background microbiota of 5 norwegian small-scale cheese production sites was examined and the effect of the isolated strains on the growth and survival of listeria monocytogenes was investigated. samples were taken from the air, food contact surfaces (storage surfaces, cheese molds, and brine) and noncontact surfaces (floor, drains, and doors) and all isolates were identified by sequencing and morphology (mold). a total of 1,314 isolates were identified and found to belong to 55 bacterial ge ...201323891302
evaluation of conjunctival bacterial flora in a herd of goats in the midwestern united states.to identify conjunctival bacterial flora in healthy adult and newborn kid goats in the midwestern united states and to compare vaginal and ocular surface flora in dam-kid pairs.201726778666
normal bacterial conjunctival flora in the huacaya alpaca (vicugna pacos).to describe the bacterial flora of the normal conjunctiva of huacaya alpacas (vicugna pacos) and to determine the effect of age and gender on this flora.201625581469
accelerated decolorization of reactive azo dyes under saline conditions by bacteria isolated from arabian seawater sediment.presence of huge amount of salts in the wastewater of textile dyeing industry is one of the major limiting factors in the development of an effective biotreatment system for the removal of azo dyes from textile effluents. bacterial spp. capable of thriving under high salt conditions could be employed for the treatment of saline dyecontaminated textile wastewaters. the present study was aimed at isolating the most efficient bacterial strains capable of decolorizing azo dyes under high saline cond ...201222290645
staphylococcal ecosystem of kitoza, a traditional malagasy meat product.kitoza is a traditional meat product from madagascar manufactured with strips of pork or beef. the process includes a first step of salting and mixing with spices followed by sun-drying or smoking step. as salting and drying select coagulase-negative staphylococci (cns), our aim was to identify the cns species in kitoza with the objective in the future of developing indigenous starters. microbial analyses revealed that the only pathogenic bacterium enumerated was staphylococcus aureus, which was ...201728187327
characterization of staphylococci in urban wastewater treatment plants in spain, with detection of methicillin resistant staphylococcus aureus st398.the objective of this study was to determine the prevalence of staphylococcus in urban wastewater treatment plants (uwtp) of la rioja (spain), and to characterize de obtained isolates. 16 wastewater samples (8 influent, 8 effluent) of six uwtps were seeded on mannitol-salt-agar and oxacillin-resistance-screening-agar-base for staphylococci and methicillin-resistant staphylococcus aureus recovery. antimicrobial susceptibility profile was determined for 16 antibiotics and the presence of 35 antimi ...201626840519
prevalence of antibiotic resistance in coagulase-negative staphylococci from spontaneously fermented meat products and safety assessment for new starters.to provide new meat starter strains lacking antibiotic (ab) resistances, we explored the ab susceptibility in 116 coagulase-negative staphylococcus (cns) isolates from traditionally fermented sausages (n=40) manufactured with meat from conventional animal breeding, and from meat products (n=76) made from meat of animals raised in natural habitats under low- or no-antibiotic pressure. less than 50% of these cns isolates showed phenotypic resistances to at least one antibiotic (ab) by using microd ...201223072691
coagulase-negative staphylococcus species in bulk milk: prevalence, distribution, and associated subgroup- and species-specific risk factors.coagulase-negative staphylococci (cns) have become the main pathogens causing bovine mastitis in recent years. a huge variation in species distribution among herds has been observed in several studies, emphasizing the need to identify subgroup- and species-specific herd-level factors to improve our understanding of the differences in ecological and epidemiological nature between species. the use of bulk milk samples enables the inclusion of a large(r) number of herds needed to identify herd-leve ...201727865514
teat apex colonization with coagulase-negative staphylococcus species before parturition: distribution and species-specific risk factors.coagulase-negative staphylococci (cns) are the main cause of bovine intramammary infections and are also abundantly present in extramammary habitats such as teat apices. teat apex colonization (tac) with cns has already been explored in lactating dairy cows at the species level, whereas this is not true for dry cows and end-term heifers. therefore, the aim of this observational study was to describe cns tac in nonlactating dairy cows and end-term heifers in flemish dairy herds and to identify as ...201626709171
the use of nucleosides and arginine as alternative energy sources by coagulase-negative staphylococci in view of meat fermentation.the ability of coagulase-negative staphylococci (cns) to use alternative energy sources in meat may partially explain their occurrence in fermented meats. of 61 cns strains tested, all metabolized adenosine and inosine in a meat simulation medium (msm). the ability to catabolize arginine via the arginine deiminase (adi) pathway varied between strains. all tested strains of staphylococcus carnosus and staphylococcus epidermidis possessed an arca gene and showed adi activity, whereas other species ...201424387852
characterization of coagulase-negative staphylococcus species from cows' milk and environment based on bap, icaa, and meca genes and phenotypic susceptibility to antimicrobials and teat dips.the aim of this study was to investigate whether the main coagulase-negative staphylococci (cns) species involved in bovine intramammary infections (imi) possess specific characteristics that promote colonization of the udder. virulence markers associated with biofilm formation, antimicrobial resistance, and biocide tolerance were compared between typically contagious cns species (staphylococcus chromogenes, staphylococcus epidermidis, staphylococcus haemolyticus, and staphylococcus simulans) an ...201222999285
high prevalence of cfr-producing staphylococcus species in retail meat in guangzhou, china.the emergence and wide distribution of the transferable gene for linezolid resistance, cfr, in staphylococci of human and animal origins is of great concern as it poses a serious threat to the public health. in the present study, we investigated the emergence and presence of the multiresistance gene, cfr, in retail meat sourced from supermarkets and free markets of guangzhou, china.201424913069
amino acid conversions by coagulase-negative staphylococci in a rich medium: assessment of inter- and intraspecies heterogeneity.the ability of coagulase-negative staphylococci (cns) to convert amino acids into volatile compounds and biogenic amines was investigated after 24h and 48 h of incubation in a rich medium (brain heart infusion). volatile compounds were measured with static-headspace gas chromatography and mass spectrometry (sh-gc-ms); biogenic amine measurements were carried out with a newly developed method based on ultra-performance liquid chromatography coupled to tandem mass spectrometry (uplc-ms/ms). in tot ...201525991555
safety and technological characterization of staphylococcus equorum isolates from jeotgal, a korean high-salt-fermented seafood, for starter development.to select starters for jeotgal, a traditional korean high-salt-fermented seafood, the safety and technological properties of its predominant bacteria isolates, which were identified as staphylococcus equorum, were assessed. among the 185 s. equorum isolates from jeotgal, 126 ampicillin-sensitive strains were subjected to assessments for antibiotic susceptibility and safety hazards. sixty-six out of the 126 s. equorum strains exhibited phenotypic resistances to at least one antibiotic, and their ...201425106039
multiresistance of staphylococcus xylosus and staphylococcus equorum from slovak bryndza cheese.staphylococcus xylosus, staphylococcus equorum, and staphylococcus epidermidis strains were isolated from bryndza cheese and identified using pcr method. the antimicrobial susceptibility of these strains was assessed using disc diffusion method and broth microdilution method. the highest percentage of resistance was detected for ampicillin and oxacillin, and in contrary, isolates were susceptible or intermediate resistant to ciprofloxacin and chloramphenicol. fourteen of the s. xylosus isolates ...201424142792
technological and safety characteristics of staphylococcaceae isolated from spanish traditional dry-cured sausages.the aim of this study was to determine the technological properties (nitrate reductase, proteolytic and lipolytic activities; and the ability to grow at the temperature and ph values of fermenting sausage, and at high nacl concentrations) and safety characteristics (amino acid decarboxylase and enterotoxigenic activities) of 38 strains of staphylococcaceae (11 of staphylococcus epidermidis, 15 of staphylococcus equorum, 5 of staphylococcus pasteuri and 7 of staphylococcus saprophyticus) isolated ...201323122502
microbial diversity of different modified atmosphere packed pot-stewed duck wings products during 8°c storage.the objective of this study was to investigate the effect of spices and packaging films on the bacterial dynamics of pot-stewed duck wings (psdw) during storage at 8°c. the results showed that spices added at a 1 : 1 ratio (weight ratio of pungent spices and fragrant spices) could delay the lag period of bacterial growth and extend the shelf-life of psdw. denaturing gel gradient electrophoresis (dgge) analysis indicated that the predominant bacteria in psdw at the end of storage were weisella he ...201728191705
staphylococci isolated from ready-to-eat meat - identification, antibiotic resistance and toxin gene profile.the aim of this study was to analyse the staphylococci isolated from ready-to-eat meat products, including pork ham, chicken cold cuts, pork sausage, salami and pork luncheon meat, sliced in the store to the consumer's specifications, along with species identification and determination of antibiotic resistance. genes encoding staphylococcal enterotoxins, staphylococcal enterotoxin-like proteins, exfoliative toxins, and toxic shock syndrome toxin 1 were also investigated. from the 41 samples, 75 ...201627614422
characterization and technological features of autochthonous coagulase-negative staphylococci as potential starters for portuguese dry fermented sausages.the manufacture of dry fermented sausages is an important part of the meat industry in southern european countries. these products are usually produced in small shops from a mixture of pork, fat, salt, and condiments and are stuffed into natural casings. meat sausages are slowly cured through spontaneous fermentation by autochthonous microbiota present in the raw materials or introduced during manufacturing. the aim of this work was to evaluate the technological and safety features of coagulase- ...201627095684
staphylococci related to farm ostriches and their sensitivity to enterocins.in slovakia, ostriches are reared mainly for their meat. there is still limited information related to microflora of ostriches, including staphylococci. knowing the composition of microflora is very important for the recognition of potential pathogenic agents. recently, a frequent problem in animals is the occurrence of bacteria resistant to antibiotics. the aim of this study was to detect staphylococcal species in feces of farm ostriches and to test their sensitivity to antibiotics and enteroci ...201626854524
complete genome sequence of staphylococcus equorum ks1039 isolated from saeu-jeotgal, korean high-salt-fermented seafood.staphylococcus equorum ks1039 was isolated from a form of traditional korean high-salt-fermented seafood called saeu-jeotgal, and exhibited growth at a nacl (w/v) concentration of 25%. comparative genome analyses with two other strains revealed the presence of two potassium voltage-gated channel genes uniquely in ks1039, which might be involved in salt tolerance. this first complete genome sequence of the species will increase our understanding of the genetic factors allowing it to be safely con ...201626718562
bacterial community dynamics of salted and fermented shrimp based on denaturing gradient gel electrophoresis.the korean traditional seafood jeotgal is consumed directly or as an additive in other foods to improve flavor or fermentation efficiency. saeujot, made from salted and fermented tiny shrimp (sfs; acetes japonicus), is the best-selling jeotgal in korea. in this study, we reveal the microbial diversity and dynamics in naturally fermented shrimp by denaturing gradient gel electrophoresis (dgge). the population fingerprints of the predominant microbiota and its succession were generated by dgge ana ...201425393163
genetic diversity of staphylococcus equorum isolates from saeu-jeotgal evaluated by multilocus sequence typing.staphylococcus equorum, the predominant bacterial species detected in saeu-jeotgal, a korean high-salt fermented seafood, is a candidate starter bacterium for saeu-jeotgal fermentation. a multilocus sequence typing (mlst) scheme was developed to evaluate the genetic diversity and background of s. equorum strains isolated from saeu-jeotgal. a total of 135 strains, including 117 isolates from saeu-jeotgal, and others from myeolchi-jeotgal, sausage, cheese and horse skin, were subjected to mlst, an ...201425103946
microbiological and physicochemical characterization of dry-cured halal goat meat. effect of salting time and addition of olive oil and paprika covering.the objective of this work was to define a simple technological process for dry-cured halal goat meat elaboration. the aims of this study were to analyze physicochemical parameters and to enumerate the microbial population at the end of the different manufacturing processes (two salting times and the addition of olive oil and paprika covering) on 36 units of meat product. a total of 532 strains were isolated from several selective culture media and then identified using classical and molecular m ...201424950081
halophilic bacteria are colonizing the exhibition areas of the capuchin catacombs in palermo, italy.the capuchin catacombs of palermo, italy, contain over 1800 mummies dating from the 16th to 20th centuries ad. their environment is not conducive to the conservation of the remains due to, among other factors, water infiltration, which is producing salt efflorescences on the walls. a multiphasic approach was applied to investigate the halophilic microbiota present in the catacombs. enrichment cultures were conducted on media containing different nacl concentrations, ranging from 3 to 20 %. for s ...201424863363
application of different molecular techniques for characterization of catalase-positive cocci isolated from sucuk.this study was carried out for the characterization and discrimination of the indigenous gram positive, catalase-positive cocci (gcc) population in sucuk, a traditional turkish dry-fermented sausage. sucuk samples, produced by the traditional method without starter culture were collected from 8 local producers in kayseri/turkey and a total of 116 gcc isolates were identified by using different molecular techniques. two different molecular fingerprinting methods; namely, randomly amplified polymo ...201424410408
genome sequence analysis of staphylococcus equorum bovine mastitis isolate umc-cns-924.intramammary infections in dairy cattle are frequently caused by staphylococci, resulting in mastitis and associated economic losses. a draft genome sequence was determined for staphylococcus equorum umc-cns-924, isolated from the milk of a holstein cow, to better understand the genetic basis of its pathogenesis and adaptation to the bovine mammary gland.201324136848
a proposal to unify two subspecies of staphylococcus equorum: staphylococcus equorum subsp. equorum and staphylococcus equorum subsp. linens.twelve isolates from jeotgal, a korean high-salt-fermented seafood, identified as staphylococcus equorum were compared by phenotypic and genotypic methods to determine their precise taxonomic identities at the subspecies level. four strains and three strains had complete 16s rrna gene sequence matches with s. equorum subsp. equorum dsm 20674(t) and s. equorum subsp. linens dsm 15097(t), respectively. five strains showed 99.9 % identity with the sequences of both type strains. in our dna-dna hybr ...201324057981
cervical spondylodiscitis due to staphylococcus equorum. 201323849315
unraveling the microbiota of teat apices of clinically healthy lactating dairy cows, with special emphasis on coagulase-negative staphylococci.swab samples (n=72) obtained from the teat apex of lactating dairy cows without visual signs of inflammation (n=18) were gathered on 2 well-managed flemish dairy herds (herds 1 and 2) during the same month to assess the bacterial diversity of teat apices before milking. a combination of both culture-dependent [plating and (gtg)(5)-pcr fingerprinting of the colonies] and culture-independent [denaturing gradient gel electrophoresis (pcr-dgge)] techniques indicated that the teat apices contain a wi ...201323313004
genome sequence of staphylococcus equorum subsp. equorum mu2, isolated from a french smear-ripened cheese.staphylococcus equorum subsp. equorum is a member of the coagulase-negative staphylococcus group and is frequently isolated from fermented food products and from food-processing environments. it contributes to the formation of aroma compounds during the ripening of fermented foods, especially cheeses and sausages. here, we report the draft genome sequence of staphylococcus equorum subsp. equorum mu2 to provide insights into its physiology and compare it with other staphylococcus species.201222933766
rna-based amplicon sequencing reveals microbiota development during ripening of artisanal versus industrial lard d'arnad.valle d'aosta lard d'arnad is a protected designation of origin (pdo) product produced from fat of the shoulder and back of heavy pigs. its manufacturing process can be very diverse, especially regarding the maturation temperature and the nacl concentration used for the brine; thereby, the main goal of this study was to investigate the impact of those parameters on the microbiota developed during curing and ripening. three farms producing lard d'arnad were selected. two plants, reflecting the in ...201728600315
biosynthesis of silver nanoparticles using artemisia annua callus for inhibiting stem-end bacteria in cut carnation flowers.a biological method for synthesising silver nanoparticles (agnps) was developed using the callus extracts from artemisia annua l. under sunlight at 25,000 lx. the agnps were characterised using transmission electron microscopy, atomic force microscope, x-ray diffraction and fourier transform infrared spectroscopy. the agnps were mostly spherical with the size of 2.1 to 45.2 nm (average 10.9 nm). pulse treatments of agnps at 125, 250 and 500 mg/l for 1 h extended vase life of cut carnation (diant ...201728477002
microbial analysis of map pot-stewed duck wings under different conditions during 15 °c storage.this study was to investigate the changes of microbial community and counts of map pot-stewed duck wing (psdw) under different packaging films and spices ratio during 15 °c storage, using the traditional bacterial cultivation and pcr-dgge. results of microbial counting showed that the shelf-life of pdsw during 15 °c storage for recommendation was within six days, and the packaging films and spices ratio didn't affect the change of microbial numbers in psdw during storage. pcr-dgge analysis revea ...201728416856
distribution of non-aureus staphylococci species in udder quarters with low and high somatic cell count, and clinical mastitis.the effect of non-aureus staphylococci (nas) in bovine mammary health is controversial. overall, nas intramammary infections (imi) increase somatic cell count (scc), with an effect categorized as mild, mostly causing subclinical or mild to moderate clinical mastitis. however, based on recent studies, specific nas may affect the udder more severely. some of these apparent discrepancies could be attributed to the large number of species that compose the nas group. the objectives of this study were ...201728456402
genomic insights into the virulence and salt tolerance of staphylococcus equorum.to shed light on the genetic background behind the virulence and salt tolerance of staphylococcus equorum, we performed comparative genome analysis of six s. equorum strains. data on four previously published genome sequences were obtained from the ncbi database, while those on strain km1031 displaying resistance to multiple antibiotics and strain c2014 causing haemolysis were determined in this study. examination of the pan-genome of five of the six s. equorum strains showed that the conserved ...201728710456
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