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klebsiella singaporensis sp. nov., a novel isomaltulose-producing bacterium.cells of strain lx3(t), isolated from soil, were gram-negative, facultatively anaerobic, non-motile, capsulated and non-endospore-forming straight rods, able to grow at 10 degrees c, unable to produce gas from lactose at 45 degrees c and unable to produce indole. the isolate converted sucrose to isomaltulose and did not produce detectable glucose by-products. the g+c content of the dna was 56.4 mol%. furthermore, comparison of 16s rrna and rpob gene sequences showed that the isolate clearly belo ...200415545446
an investigation into laboratory misidentification of a bloodstream klebsiella variicola infection. 201526063851
single amino acid substitution in the pullulanase of klebsiella variicola for enhancing thermostability and catalytic efficiency.based on conserved sites and homology modeling analysis, the residue phe581 in the klebsiella variicola shn-1 pullulanase was selected as the potential thermostability-related site and its role on thermostability and activity was investigated by site-saturated mutagenesis. compared with the wild-type pullulanase, the optimum temperature of the mutants including f581l, f581q, f581r, f581t, f581v, and f581y was increased from 53 to 56 °c, and correspondingly the half lives of these mutants at 55 ° ...201526018345
draft genome sequence of the first hypermucoviscous klebsiella variicola clinical isolate.an antibiotic-susceptible and hypermucoviscous clinical isolate of klebsiella variicola (k. variicola 8917) was obtained from the sputum of an adult patient. this work reports the complete draft genome sequence of k. variicola 8917 with 103 contigs and an annotation that revealed a 5,686,491-bp circular chromosome containing a total of 5,621 coding dna sequences, 65 trna genes, and an average g+c content of 56.98%.201525858850
ammonia-oligotrophic and diazotrophic heavy metal-resistant serratia liquefaciens strains from pioneer plants and mine tailings.mine tailings are man-made environments characterized by low levels of organic carbon and assimilable nitrogen, as well as moderate concentrations of heavy metals. for the introduction of nitrogen into these environments, a key role is played by ammonia-oligotrophic/diazotrophic heavy metal-resistant guilds. in mine tailings from zacatecas, mexico, serratia liquefaciens was the dominant heterotrophic culturable species isolated in n-free media from bulk mine tailings as well as the rhizosphere, ...201627138047
complete genome sequence of klebsiella variicola strain hkuopla, a cellulose-degrading bacterium isolated from giant panda feces.we report here the complete genome sequence of klebsiella variicola strain hkuopla, isolated from a giant panda feces sample collected from ocean park, hong kong. the complete genome of this bacterium may contribute toward the discovery of efficient cellulose-degrading pathways.201526472841
first detection of klebsiella variicola producing oxa-181 carbapenemase in fresh vegetable imported from asia to switzerland.the emergence and worldwide spread of carbapenemase-producing enterobacteriaceae is of great concern to public health services. the aim of this study was to investigate the occurrence of carbapenemase-producing enterobacteriaceae in fresh vegetables and spices imported from asia to switzerland.201526448862
emergence of klebsiella variicola positive for ndm-9, a variant of new delhi metallo-β-lactamase, in an urban river in south korea.to examine the presence of pathogenic bacteria carrying new delhi metallo-β-lactamase in the environment and to characterize the genome structures of these strains.201728087584
erratum to: development of a multiplex-pcr probe system for the proper identification of klebsiella variicola. 201626984789
draft genome sequence of klebsiella variicola strain kv321 isolated from rhizosphere soil of pisolithus tinctorius-eucalyptus mycorrhiza.the draft genome sequences of klebsiella variicola strain kv321, which was isolated from rhizosphere soil of pisolithus tinctorius-eucalyptus mycorrhiza, are reported here. the genome sequences contain genes involved in abc transporter function in multiple-antibiotic drug resistance and colonization. this genomic analysis will help understand the genomic basis of k. variicola virulence genes and how the genes play a part in its interaction with other living organisms.201627445373
genome sequences of klebsiella variicola isolates from dairy animals with bovine mastitis from newfoundland, canada.klebsiella variicola was recently reported as an emerging and/or previously misidentified species associated with opportunistic infections in humans. here, we report the draft genome sequences of k. variicola isolates from two animals with clinical mastitis from a dairy farm in newfoundland, canada.201526358587
draft genome sequences of klebsiella variicola plant isolates.three endophytic klebsiella variicola isolates-t29a, 3, and 6a2, obtained from sugar cane stem, maize shoots, and banana leaves, respectively-were used for whole-genome sequencing. here, we report the draft genome sequences of circular chromosomes and plasmids. the genomes contain plant colonization and cellulases genes. this study will help toward understanding the genomic basis of k. variicola interaction with plant hosts.201526358599
imi-2 carbapenemase in a clinical klebsiella variicola isolated in the uk. 201728379381
fatal sepsis caused by an unusual klebsiella species that was misidentified by an automated identification system.this is a description of fatal sepsis caused by infection with klebsiella variicola, which is an isolate genetically related to klebsiella pneumoniae. the patient's condition was incorrectly diagnosed as common sepsis caused by k. pneumoniae, which was identified using an automated identification system, but next-generation sequencing and the non-fermentation of adonitol finally identified the cause of sepsis as k. variicola.201323449877
description of klebsiella quasipneumoniae sp. nov., isolated from human infections, with two subspecies, klebsiella quasipneumoniae subsp. quasipneumoniae subsp. nov. and klebsiella quasipneumoniae subsp. similipneumoniae subsp. nov., and demonstration that klebsiella singaporensis is a junior heterotypic synonym of klebsiella variicola.strains previously classified as members of klebsiella pneumoniae phylogroups kpi, kpii-a, kpii-b and kpiii were characterized by 16s rrna (rrs) gene sequencing, multilocus sequence analysis based on rpob, fusa, gapa, gyra and leus genes, average nucleotide identity and biochemical characteristics. phylogenetic analysis demonstrated that kpi and kpiii corresponded to k. pneumoniae and klebsiella variicola, respectively, whereas kpii-a and kpii-b formed two well-demarcated sequence clusters disti ...201424958762
the genome of a clinical klebsiella variicola strain reveals virulence-associated traits and a pl9-like plasmid.klebsiella species frequently cause clinically relevant human infections worldwide. we report the draft genome sequence of a brazilian clinical isolate (bz19) of the recently recognized species klebsiella variicola. the comparison of bz19 genome content with the at-22 (environmental k. variicola) and several clinical klebsiella pneumoniae shows that these species share a set of virulence-associated determinants. of note, this k. variicola strain harbours a plasmid-like element that shares the sa ...201425135672
klebsiella variicola is a frequent cause of bloodstream infection in the stockholm area, and associated with higher mortality compared to k. pneumoniae.clinical isolates of klebsiella pneumoniae are divided into three phylogroups and differ in their virulence factor contents. the aim of this study was to determine an association between phylogroup, virulence factors and mortality following bloodstream infection (bsi) caused by klebsiella pneumoniae. isolates from all adult patients with bsi caused by k. pneumoniae admitted to karolinska university hospital, solna between 2007 and 2009 (n = 139) were included in the study. phylogenetic analysis ...201425426853
complete genome sequence of endophytic nitrogen-fixing klebsiella variicola strain dx120e.klebsiella variicola strain dx120e (=cgmcc 1.14935) is an endophytic nitrogen-fixing bacterium isolated from sugarcane crops grown in guangxi, china and promotes sugarcane growth. here we summarize the features of the strain dx120e and describe its complete genome sequence. the genome contains one circular chromosome and two plasmids, and contains 5,718,434 nucleotides with 57.1% gc content, 5,172 protein-coding genes, 25 rrna genes, 87 trna genes, 7 ncrna genes, 25 pseudo genes, and 2 crispr re ...201526203334
genomic identification of nitrogen-fixing klebsiella variicola, k. pneumoniae and k. quasipneumoniae.it was difficult to differentiate klebsiella pneumoniae, k. quasipneumoniae and k. variicola by biochemical and phenotypic tests. genomics increase the resolution and credibility of taxonomy for closely-related species. here, we obtained the complete genome sequence of the k. variicola type strain dsm 15968(t) (=f2r9(t)). the genome of the type strain is a circular chromosome of 5,521,203 bp with 57.56% gc content. from 540 klebsiella strains whose genomes had been publicly available as at 3 mar ...201626471769
identification and characterization of imipenem-resistant klebsiella pneumoniae and susceptible klebsiella variicola isolates obtained from the same patient.klebsiella variicola, a bacterium closely genetically related to klebsiella pneumoniae, is commonly misidentified as k. pneumoniae by biochemical tests. to distinguish between the two bacteria, phylogenetic analysis of the rpob gene and the identification of unique genes in both bacterial species by multiplex-polymerase chain reaction (pcr) provide the means to reliably identify and genotype k. variicola. in recent years, k. variicola has been described both as the cause of an intrahospital outb ...201626571390
a one-step multiplex pcr to identify klebsiella pneumoniae, klebsiella variicola, and klebsiella quasipneumoniae in the clinical routine.klebsiella pneumoniae, klebsiella variicola and klebsiella quasipneumoniae are difficult to differentiate phenotypically, leading to misinterpretation of their infection prevalence. we propose a multiplex pcr for blashv, blalen and blaokp and their flanking gene (deor). since this scheme focuses only on chromosomal genes, it will be feasible for klebsiella identification in the clinical routine.201728139276
amoxicillin-clavulanic acid resistance in fecal enterobacteriaceae from patients with cystic fibrosis and healthy siblings.the present study set out to detect and identify amoxicillin-clavulanic acid (amc)-resistant enterobacteriaceae in fecal samples of two patients with cystic fibrosis (cf) and their respective siblings.201323867070
the overproduction of indole-3-acetic acid (iaa) in endophytes upregulates nitrogen fixation in both bacterial cultures and inoculated rice plants.endophytic bacteria from roots and leaves of rice plants were isolated and identified in order to select the diazotrophs and improve their nitrogen-fixing abilities. the nitrogen-fixing endophytes were identified by pcr amplification of the nifh gene fragment. for this purpose, two isolates, enterobacter cloacae rca25 and klebsiella variicola rca26, and two model bacteria (herbaspirillum seropedicae z67 and sinorhizobium fredii ngr234) were transformed to increase the biosynthesis of the main pl ...201728197647
development of a multiplex-pcr probe system for the proper identification of klebsiella variicola.klebsiella variicola was very recently described as a new bacterial species and is very closely related to klebsiella pneumoniae; in fact, k. variicola isolates were first identified as k. pneumoniae. therefore, it might be the case that some isolates, which were initially classified as k. pneumoniae, are actually k. variicola. the aim of this study was to devise a multiplex-pcr probe that can differentiate isolates from these sister species.201525886267
klebsiella alba sp. nov., a novel pesticide-tolerant bacterium from a heavily polluted environment.a novel gram-negative, aerobic, rod-shaped and pesticide (chlorpyrifos and atrazine) tolerant strain, designated cw-d 3(t), was isolated from a heavily polluted soil sample in jiangsu province, china. the strain could tolerate about 800 microg.ml(-1) atrazine or chlorpyrifos when added in tyb medium. a polyphasic taxonomy including phylogenetic and phenotypic analysis was performed on the new isolate. phylogenetic analysis and chemotaxonomic characteristics revealed that strain cw-d 3(t) belongs ...201020647681
signal peptide-independent secretory expression and characterization of pullulanase from a newly isolated klebsiella variicola shn-1 in escherichia coli.a strain with the power to produce extracellular pullulanase was obtained from the sample taken from a flour mill. by sequencing its 16s rdna, the isolate was identified as klebsiella variicola shn-1. when the gene encoding pullulanase, containing the n-terminal signal sequence, was cloned into escherichia coli bl21 (de3), extracellular activity was detected up to 10 u/ml, a higher level compared with the results in published literature. subsequently, the recombinant pullulanase was purified and ...201323129508
ethanol production from glycerol-containing biodiesel waste by klebsiella variicola shows maximum productivity under alkaline conditions.biodiesel fuel (bdf) waste contains large amounts of crude glycerol as a by-product, and has a high alkaline ph. with regard to microbial conversion of ethanol from bdf-derived glycerol, bacteria that can produce ethanol at alkaline ph have not been reported to date. isolation of bacteria that shows maximum productivity under alkaline conditions is essential to effective production of ethanol from bdf-derived glycerol. in this study, we isolated the klebsiella variicola tb-83 strain, which demon ...201424681408
partial characterization and flocculating behavior of an exopolysaccharide produced in nutrient-poor medium by a facultative oligotroph klebsiella sp. pb12.a facultative oligotrophic strain from the water sample of river mahananda, siliguri india was selected for its property to produce exopolysaccharide (eps) in nutrient-poor (oligotrophic) medium. viability assay of the strain was performed in sterile liquid lb, r2a, river water and diluted (10(-3)) lb at 30°c and ph 7 to understand oligotrophy. the selected strain was identified by 16s rrna gene sequencing and designated as klebsiella sp. pb12. phylogenetic analysis showed its closest relationsh ...201322944200
improved ethanol tolerance and ethanol production from glycerol in a streptomycin-resistant klebsiella variicola mutant obtained by ribosome engineering.to improve the ethanol tolerance of the klebsiella variicola strain tb-83, we obtained the streptomycin-resistant, ethanol-tolerant mutant strain tb-83d by a ribosome engineering approach. strain tb-83d was able to grow in the presence of 7% (v/v) ethanol and it showed higher ethanol production than strain tb-83. examination of various culture conditions revealed that yeast extract was essential for ethanol production and bacterial growth. in addition, ethanol production was elevated to 32g/l by ...201525460997
sources of klebsiella and raoultella species on dairy farms: be careful where you walk.klebsiella spp. are a common cause of mastitis, milk loss, and culling on dairy farms. control of klebsiella mastitis is largely based on prevention of exposure of the udder to the pathogen. to identify critical control points for mastitis prevention, potential klebsiella sources and transmission cycles in the farm environment were investigated, including oro-fecal transmission, transmission via the indoor environment, and transmission via the outdoor environment. a total of 305 samples was coll ...201121257074
identification of gram-negative bacteria and genetic resistance determinants from positive blood culture broths by use of the verigene gram-negative blood culture multiplex microarray-based molecular assay.bloodstream infection is a serious condition associated with significant morbidity and mortality. the outcome of these infections can be positively affected by the early implementation of effective antibiotic therapy based on the identification of the infecting organism and genetic markers associated with antibiotic resistance. in this study, we evaluated the microarray-based verigene gram-negative blood culture (bc-gn) assay in the identification of 8 genus or species targets and 6 genetic resi ...201525994165
[growth-promoting effect of inoculating klebsiella variicola dx120e on different sugarcane cultivars].in order to investigate the growth promoting effect of inoculating klebsiella variicola dx120e, a bacterial strain with high activity of associative nitrogen fixation, on sugarcane, the strain was inoculated through roots into the pathogen free micropropagated seedlings of two sugarcane cultivars b8 and gt21. the bacterial numbers colonized in sugarcane plants, the activities of the key enzymes for nitrogen metabolism, the nitrate concentration and nutrient uptake were analyzed. the results indi ...201425345062
identification of clinical isolates of indole-positive and indole-negative klebsiella spp.biochemical methods employed to classify bacterial species have limitations and may have contributed to the taxonomic complexity recently reported for the genus klebsiella. the objective of the present study was to apply a simple biochemical test panel to classify a collection of human klebsiella isolates. we found that with only three additional tests, it is possible to place most isolates in a defined species. analysis of a 512-bp sequence of the rpob gene was used as the reference. a total of ...200616928968
peptide nucleic acid fluorescence in situ hybridization for rapid detection of klebsiella pneumoniae from positive blood cultures.this study evaluated a novel peptide nucleic acid (pna) probe targeting a region of the 23s rrna gene of klebsiella pneumoniae by fluorescence in situ hybridization (fish). analytical performance was determined using 39 reference strains and other well-characterized strains of klebsiella spp. and enterobacter aerogenes. the probe was found to be specific for the k. pneumoniae complex (k. pneumoniae including klebsiella ozaenae and klebsiella variicola). the diagnostic accuracy was evaluated with ...200717577055
klebsiella variicola, a novel species with clinical and plant-associated isolates.a new klebsiella species, k. variicola, is proposed on the basis of total dna-dna hybridization, on the monophyly observed in the phylogenetic analysis derived from the sequences of rpob, gyra, mdh, infb, phoe and nifh genes and on distinct phenotypic traits. the bacteria from this new species seem to be genetically isolated from k. pneumoniae strains, do not ferment adonitol and were obtained from plants (such as banana, rice, sugar cane and maize) and hospitals. the type strain is f2r9t (= atc ...200415053318
metataxonomic profiling and prediction of functional behaviour of wheat straw degrading microbial consortia.mixed microbial cultures, in which bacteria and fungi interact, have been proposed as an efficient way to deconstruct plant waste. the characterization of specific microbial consortia could be the starting point for novel biotechnological applications related to the efficient conversion of lignocellulose to cello-oligosaccharides, plastics and/or biofuels. here, the diversity, composition and predicted functional profiles of novel bacterial-fungal consortia are reported, on the basis of replicat ...201424955113
distribution of the putative type vi secretion system core genes in klebsiella spp.the type vi secretion system (t6ss) is a recently characterized secretion system which appears to be involved in bacterial pathogenesis as a potential nano-syringe for the translocation of effector proteins into the eukaryotic host cell cytoplasm. until now no evidence was provided for the presence of t6ss in the genomes of the sequenced representatives of klebsiella spp., including the human opportunistic pathogen klebsiella pneumoniae. however, in a previous study by lawlor et al. (2005), were ...201020932940
effects of ensiling fermentation and aerobic deterioration on the bacterial community in italian ryegrass, guinea grass, and whole-crop maize silages stored at high moisture content.the effects of storage period and aerobic deterioration on the bacterial community were examined in italian ryegrass (ir), guinea grass (gg), and whole-crop maize (wm) silages. direct-cut forages were stored in a laboratory silo for 3, 7, 14, 28, 56, and 120 d without any additives; live counts, content of fermentation products, and characteristics of the bacterial community were determined. 2,3-butanediol, acetic acid, and lactic acid were the dominant fermentation products in the ir, gg, and w ...201325049913
degradation of euptox a by tannase-producing rumen bacteria from migratory goats.the gut microbiota capable of degrading plant biomass and anti-nutritional phytometabolites are of immense importance. this study reports isolation and characterization of tannase-producing rumen bacteria that could also degrade euptox a (9-oxo-10,11-dehydroageraphorone) present in eupatorium adenophorum (spreng).201728801990
enhanced bioremoval of lead by earthworm-lumbricus terrestris co-cultivated with bacteria-klebsiella variicola.lead is a toxic heavy metal having devastating effects on the environment. the current study was focussed on bioremoval of lead using earthworm and lead resistant bacteria. earthworms were subjected to various concentrations of lead in the soil bioaugmented with lead resistant bacteria (vitmvcj1) to enhance the uptake of lead from the contaminated soil. significant increase was observed in the length and body weight of the earthworms supplemented with lead resistant bacteria. similarly, there wa ...201728850872
whole-genome sequencing of human clinical klebsiella pneumoniae isolates reveals misidentification and misunderstandings of klebsiella pneumoniae, klebsiella variicola, and klebsiella quasipneumoniae.klebsiella pneumoniae is a major threat to public health, causing significant morbidity and mortality worldwide. the emergence of highly drug-resistant strains is particularly concerning. there has been a recognition and division of klebsiella pneumoniae into three distinct phylogenetic groups: klebsiella pneumoniae, klebsiella variicola, and klebsiella quasipneumoniae. k. variicola and k. quasipneumoniae have often been described as opportunistic pathogens that have less virulence in humans tha ...201728776045
novel consortium of klebsiella variicola and lactobacillus species enhances the functional potential of fermented dairy products by increasing the availability of branched-chain amino acids and the amount of distinctive volatiles.identify novel bacterial taxa that could increase the availability of branched-chain amino acids and the amount of distinctive volatiles during skim milk fermentation.201728815819
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