| hydrocarboniphaga effusa gen. nov., sp. nov., a novel member of the gamma-proteobacteria active in alkane and aromatic hydrocarbon degradation. | novel alkane-degrading strains of bacteria were isolated from soil contaminated with fuel oil from a leaking underground tank in new jersey, usa. two phenotypically similar strains (designated ap102 and ap103t) possessed 16s rrna sequences unique among the majority of known hydrocarbon-degrading bacteria. the 16s rrna sequences showed a moderate but distant relationship to the genus nevskia and a substantial similarity to strains that had previously been isolated for growth on phenol (in japan) ... | 2004 | 15280292 |
| profiling mechanisms of alkane hydroxylase activity in vivo using the diagnostic substrate norcarane. | mechanistically informative chemical probes are used to characterize the activity of functional alkane hydroxylases in whole cells. norcarane is a substrate used to reveal the lifetime of radical intermediates formed during alkane oxidation. results from oxidations of this probe with organisms that contain the two most prevalent medium-chain-length alkane-oxidizing metalloenzymes, alkane omega-monooxygenase (alkb) and cytochrome p450 (cyp), are reported. the results--radical lifetimes of 1-7 ns ... | 2007 | 17317570 |
| solimonas soli gen. nov., sp. nov., isolated from soil of a ginseng field. | a micro-organism, dcy12t, comprising gram-negative, non-motile, pale-yellow rods was isolated from soil from a ginseng field in south korea and was investigated to determine its taxonomic status. it grew optimally at 30 degrees c and at ph 7.0, the g+c content of its dna was 40.5 mol%, the major components of the fatty acid profile were c16:0 and c18:1 and the major ubiquinone was q-8. a phylogenetic analysis based on the 16s rrna gene sequence revealed that the novel isolate was most closely re ... | 2007 | 17978223 |
| description of sinobacter flavus gen. nov., sp. nov., and proposal of sinobacteraceae fam. nov. | a novel gram-negative, non-motile, rod-shaped bacterium, designated cw-kd 4(t), was isolated from a polluted soil sample collected from jiangsu province, china, by using a classic enrichment method. based on 16s rrna gene sequence analysis, the novel strain represented a deep-rooting lineage within the class gammaproteobacteria that was clustered with the genera hydrocarboniphaga and nevskia and some other unidentified bacteria. polyphasic taxonomic studies revealed that strain cw-kd 4(t) showed ... | 2008 | 18175707 |
| steroidobacter denitrificans gen. nov., sp. nov., a steroidal hormone-degrading gammaproteobacterium. | a denitrifying bacterium, designated strain fs(t), was isolated from anoxic digested sludge on oestradiol [17beta-oestra-1,3,5(10)-triene-3,17-diol] or testosterone (17beta-hydroxyandrost-4-en-3-one) as the sole source of carbon and energy with nitrate as the electron acceptor. strain fs(t) represents the first known bacterium to grow anaerobically on both oestradiol (c-18) and testosterone (c-19). steroidal hormones were degraded completely by nitrate reduction to dinitrogen monoxide, which was ... | 2008 | 18768632 |
| hydrocarboniphaga daqingensis sp. nov., isolated from a freshwater lake. | a gram-negative bacterium, designated b2-9(t), was isolated from water of a slightly alkaline lake, longhu lake in daqing, north-east china. strain b2-9(t) was an aerobic rod, heterotrophic, catalase- and oxidase-positive and motile by means of a single polar flflagellum. the isolate grew well on n-alkanes c(9)-c(17) and weakly on c(6)-c(8), c(18) and c(19). growth occurred at 10-37 °c (optimum 20-25 °c), at ph 5.5-9.5 (optimum ph 7.0) and with ≤1.0 % (w/v) nacl. the major fatty acids were c(18 : ... | 2011 | 20348325 |
| panacagrimonas perspica gen. nov., sp. nov., a novel member of gammaproteobacteria isolated from soil of a ginseng field. | a taxonomic study was carried out on gsoil 142(t), a bacterial strain isolated from the soil collected in a ginseng field in pocheon province, south korea. comparative 16s rrna gene sequence studies showed a clear affiliation of this bacterium to the gammaproteobacteria, and it was most closely related to hydrocarboniphaga effusa atcc baa 332(t) (94.4%, 16s rrna gene sequence similarity), nevskia ramosa dsm 11499(t) (94.1%) and alkanibacter difficilis mn154.3(t) (92.0%). strain gsoil 142(t) was ... | 2010 | 20437162 |