| [effect of bradkinin-potentiating snake venom peptides and c-terminal pentapeptide fragment of bradkinin on carboxypeptidase n and kiniase activities of human blood serum]. | effects of bradikinin-potentiating peptides (bpp5a and bppb), which are identical to peptides from bothrops jararaca and agkistrodon halys blomhoffii snake venom, and pentapeptide c-terminal bradikinin fragment on carboxypeptidase n and kinase activities in human blood serum are studied. it is found that bpp5a and bppb at concentrations of 5-10(-4) m and 2.5-10(-4) m respectively do not inhibit the hydrolysis of hippuryl-l-lysine and hippuryl-l-argininic acid by carboxypeptidase n and at a conce ... | 1975 | 173428 |
| [the effect of potentiating factors on the bradykinine effect in vitro]. | bradykinin potentiating factors from the venom of bothrops jajaraca and agkistrodon halys blomhoffii potentiate the action of bradykinin at several smooth muscles. this potentiation is specific for bradykinin and has to be distinguished from an unspecific potentiation. the potentiation induced by bpf is not due to an indirect cholinergic mechanism or to a kininase inhibition in vitro. the results suggest that there would be an allosteric transition of the bradykinin receptor. | 1976 | 1033653 |
| synthesis and properties of new bradykinin potentiating peptides. | in a study of the relationship between structure and activity of bradykinin potentiating peptides (bpp), six analogs and homologs of peptides occurring in the venoms of bothrops jararaca and agkistrodon halys blomhoffii were synthesized and assayed in the isolated guinea pig ileum and rat uterus. none of the peptides had bradykinin-like activity and their bradykinin potentiating activity was much greater in the guinea pig ileum than in the uterus. the following observations were made with the gu ... | 1975 | 1120978 |
| structure of acidic phospholipase a2 for the venom of agkistrodon halys blomhoffii at 2.8 a resolution. | the crystal structure of acidic phospholipase a2 from the venom of agkistrodon halys blomhoffii has been determined by molecular replacement methods based on the known structure of crotalus atrox pla2, a same group ii enzyme. the overall structures, except the calcium-binding regions, are very similar to each other. a calcium ion is pentagonally ligated to two carboxylate oxygen atoms of asp-49 and each carbonyl oxygen atoms of tyr-28, gly-30 and ala-31. a reason why the former enzyme functions ... | 1992 | 1567418 |
| [effect of cepharanthin on the lethality and cardiovascular disorder by mamushi, agkistrodon halys blomhoffi, snake venom]. | effects of cephranthin on the lethality to mice and the cardiovascular disorder in rat induced by mamushi (agkistrodon halys blomhoffi) snake venom were studied. the ld50 of mamushi snake venom was 1.22 mg/kg in mice by i.p. administration. cepharanthin activated slightly the lethal activity when it was administered just after injection of the venom. however, this drug was effective against the venom at 4 to 5 times the lethal dose, if it was administered at the optimal interval. mamushi snake v ... | 1991 | 1813364 |
| immunochemical cross-reactivity of two phospholipase a2 neurotoxins, agkistrodotoxin and crotoxin. | polyclonal rabbit antisera were raised against the phospholipase a2 neurotoxin agkistrodotoxin (agtx) from agkistrodon blomhoffii brevicaudus venom and against the phospholipase a2 subunit (component-b, cb) of crotoxin from crotalus durissus terrificus venom. anti-agtx antibodies cross-reacted strongly with crotoxin and crotoxin-like molecules and more weakly with other phospholipases a2 from the venoms of viperidae and crotalidae. on the other hand, anti-cb antibodies cross-reacted with agtx, a ... | 1991 | 1862520 |
| halysin, an antiplatelet arg-gly-asp-containing snake venom peptide, as fibrinogen receptor antagonist. | by means of sephadex g-75 and cm-sephadex c-50 column chromatography and reverse-phase hplc, a low molecular weight (mr = 7500), cysteine-rich peptide, halysin, was purified from agkistrodon halys (mamushi) snake venom. halysin is a potent platelet aggregation inhibitor that concentration-dependently inhibited human platelet aggregation stimulated by adp, thrombin and collagen (ic50 = 0.16 to 0.36 microm) without affecting platelet secretion. it was active in inhibiting platelet aggregation of p ... | 1991 | 1888330 |
| purification of a kininogenase (kininogenase-2) from the venom of agkistrodon caliginosus (kankoku-mamushi). | from the partially purified capillary permeability-increasing enzyme obtained from a. caliginosus venom, another kininogenase (kininogenase-2) was purified by gel filtration on sephadex g-100 and ion-exchange chromatography on cm-sephadex c-50, deae-sephadex a-50, s-sepharose fast flow and q-sepharose fast flow. the purified enzyme was homogeneous by polyacrylamide gel electrophoresis at ph 8.3 and sds-gel electrophoresis. the kininogenase-2 had arginine ester hydrolytic and capillary permeabili ... | 1991 | 2028474 |
| kinetics of the hydrolysis of mixed micelles of dipalmitoyllecithin with triton x-100 catalyzed by a phospholipase a2 from the venom of agkistrodon halys blomhoffii. | the ph dependence of kinetic parameters for the hydrolysis of mixed micelles of 1,2-dipalmitoyl-sn-glycero-3-phosphorylcholine (dic16pc) with triton x-100, catalyzed by the intact and the n-terminal alpha-nh2-modified phospholipases a2 (pla2s) of agkistrodon halys blomhoffii, was studied at 25 degrees c and ionic strength 0.1 in the presence of saturating amounts of ca2+. the ph dependence of the kinetic parameters for the hydrolysis of monodispersed dic6pc, catalyzed by the modified enzyme, was ... | 1990 | 2229006 |
| igg subclass distributions of anti-horse serum antibodies and natural venom-antibodies produced in response to antivenom injection or snake bite in humans. | the japanese mamushi (agkistrodon halys blomhoffi, boie) is the most common snake in japan. bite victims treated with antivenom (horse serum) can produce antibodies against the horse serum and the snake venom. we studied distributions of the igg subclasses of both these antibodies produced in response to antivenom injection and snake bite. we found that igg1 and igg4 of each antibody in the victims' serum were present for a long period of time. | 1990 | 2343468 |
| [pharmacological study on agkistrodon blomhoffii blomhoffii boie. ii. effect of 50% ethanolic extract on phagocytic activity of mouse reticuloendothelial system]. | effect of a 50% ethanolic extract ([m]) from dried whole body of agkistrodon blomhoffii blomhoffii boie on a phagocytic activity of reticuloendothelial system was studied by the carbon clearance method in mice. the clearance-rate of carbon was significantly shortened 1 h after the oral administration of [m] (250 or 500 mg/kg, one time/d for 3d). [m] activated the phagocytosis of carbon by macrophage (m phi) in the spleen and the phagocytosis of latex by peritoneal m phi. these results suggested ... | 1990 | 2376825 |
| isolation and fundamental properties of a phospholipase a2 inhibitor from the blood plasma of trimeresurus flavoviridis. | phospholipase a2 inhibitor was purified from the blood plasma of habu, trimeresurus flavoviridis, by sephadex g-200 gel filtration, deae-cellulose chromatography, and blue-sepharose cl-6b column chromatography. the purified inhibitor was shown to be a glycoprotein with a molecular weight of about 100k. it was found to consist of four subunits whose molecular weights were around 20-24k. in order to examine the inhibition mechanism of the inhibitor, the interaction of the inhibitor with a phosphol ... | 1989 | 2628432 |
| [pharmacological study on agkistrodon blomhoffii blomhoffii boie. i. effect of the 50% ethanolic extract on experimental gastric ulceration]. | anti-ulcerogenic activities of a 50% ethanolic extract [( m]) from whole body of agkistrodon blomhoffii blomhoffii boie were investigated in rats. [m] at oral doses of 250-500 mg/kg was found to show a prophylactic effect on the water-immersion stress- and ethanol-induced ulcer and a remedial effect on acetic acid-induced ulcer, but not shay-ulcer in pylorus-ligated rats and indomethacin-induced ulcer. [m] increased the gastric mucosal blood flow in intact rats and improved the decrease of the b ... | 1989 | 2810063 |
| purification of a kininogenase from the venom of agkistrodon caliginosus (kankoku-mamushi). | during the isolation of a capillary permeability-increasing enzyme from the venom of a. caliginosus, a kininogenase was also purified from the venom by gel filtration on sephadex g-100, ion-exchange chromatographies on cm-sephadex c-50 and deae-sephadex a-50, and gel filtration on sephadex g-75. by this procedure, 11 mg of the purified enzyme were obtained from 4 g of the venom. the purified enzyme was homogeneous by polyacrylamide disc gel electrophoresis at ph 8.3 and sodium dodecyl sulfate-po ... | 1988 | 3201479 |
| some properties of a kininogenase from the venom of agkistrodon caliginosus (kankoku-mamushi). | a kininogenase (bradykinin-releasing enzyme) from the venom of a. caliginosus, is a single polypeptide-chain glycoprotein with a mol.wt of about 33,500, which contains 10.1% carbohydrate. the isoelectric point of the enzyme is 3.5 and the enzyme has 274 amino acid residues based on the mol.wt of 33,500. the enzyme hydrolyzed arginine esters more readily than lysine esters, but did not hydrolyze tyrosine ester. the activity of the enzyme on hydrolysis of arginine ester or on liberation of kinin f ... | 1988 | 3201480 |
| the primary structure of rat platelet phospholipase a2. | in our previous report (hayakawa, m., kudo, i., tomita, m., & inoue, k. (1988) j. biochem. 103, 263-266), we have shown that phospholipases a2 purified from rat platelet membrane fractions and an extracellular medium of thrombin-stimulated rat platelets were essentially identical to each other. both purified enzymes were digested with proteases, and the resulting peptides were subjected to primary sequence determination. the sequence analysis of the hplc-separated peptides and the alignment of t ... | 1988 | 3235451 |
| amino acid sequence of a basic agkistrodon halys blomhoffii phospholipase a2. possible role of nh2-terminal lysines in action on phospholipids of escherichia coli. | a basic (pi = 10.2) phospholipase a2 of the venom of the snake agkistrodon halys blomhoffii is one of a few phospholipases a2 capable of hydrolyzing the phospholipids of escherichia coli killed by a bactericidal protein purified from human or rabbit neutrophil granules. we have shown that modification of as many as 4 mol of lysine per mole of the phospholipase a2, either by carbamylation or by reductive methylation [forst, s., weiss, j., & elsbach, p. (1982) j. biol. chem. 257, 14055-14057], had ... | 1986 | 3530322 |
| [rheological properties of the blood and erythrocyte suspension after exposure to zootoxins]. | the rheological properties of blood (viscosity, suspension stability and deformation of red blood cells) have been studied under the influence of zootoxins. it has been shown that the toxins apis mellifera, mesobuthus eupeus, agkistrodon blomhoffii in vitro exert essential effect on haemorheological indexes. | 1987 | 3593804 |
| capillary permeability-increasing enzyme from the venom of agkistrodon caliginosus (kankoku-mamushi): activity due to the release of peptide material from a protein in bovine plasma. | when a mixture of the purified capillary permeability-increasing enzyme from a. caliginosus venom and bovine plasma or heated bovine plasma was injected into the depilated skin of the back of a rabbit, the capillary permeability-increasing activity was much greater than that induced by injection of the enzyme alone. the substance which increases capillary permeability was extracted from the incubated mixture of bovine plasma and enzyme with 50-70% ethanol. its activity was lost when treated with ... | 1985 | 4039478 |
| bradykinin-potentiating peptides from the venom of agkistrodon halys blomhoffi. isolation of five bradykinin potentiators and the amino acid sequences of two of them, potentiators b and c. | | 1971 | 4323853 |
| the bradykininase activities of extracts of dog lung. | 1. homogenates of dog lungs freed from blood inactivate bradykinin. the bradykininase activity is present in soluble and particulate subcellular fractions.2. the fraction with the highest relative specific activity is that sedimenting between 8,700 g and 78,000 g. this fraction has been studied in more detail.3. there is evidence for two bradykinin inactivating enzymes in this fraction with apparent k(m) values of 7.5 mum and 120 mum.4. the bradykininase activity is not dependent on the concentr ... | 1973 | 4723801 |
| structure of potentiator a, one of the five bradykinin potentiating peptides from the venom of agkistrodon halys blomhoffii. | | 1973 | 4730295 |
| some properties of proteinase b in the venom of agkistrodon halys blomhoffii. | | 1971 | 5004066 |
| structure of bradykinin-potentiating peptide containing tryptophan from the venom of agkistrodon halys blomhoffii. | | 1970 | 5530287 |
| bradykinin-potentiating peptides from the venom of agkistrodon halys blomhoffii. | | 1969 | 5801778 |
| rabbit platelet calcium atpase differs from the human erythrocyte (ca2+ + mg2+)-atpase in its response to three purified phospholipases a2, exogenous phospholipids and calmodulin. | human erythrocyte (ca2+ + mg2+)-atpase and calcium atpase of rabbit platelets were compared by their responses to a variety of treatments. these included three purified phospholipases a2 (acidic, neutral and basic) from agkistrodon halys blomhoffii, as well as several phospholipids and lysophospholipids. the erythrocyte enzyme was stimulated 2-3-fold by all three phospholipases with maximal stimulation occurring at different concentrations of the three enzymes. the basic phospholipase was the mo ... | 1984 | 6148104 |
| a potent platelet aggregation inhibitor purified from agkistrodon halys (mamushi) snake venom. | by means of gel filtration on sephadex g-75, deae-sephadex a-50 column chromatography and three gel filtrations on sephadex g-75, a potent platelet aggregation inhibitor was purified from agkistrodon halys snake venom and shown to be a single peptide chain, as judged by sds-polyacrylamide gel electrophoresis. the purified platelet aggregation inhibitor was an acidic protein with a molecular weight of 14,000 and possessed phospholipase a2 activity. its inhibitory activity on platelet aggregation ... | 1983 | 6419392 |
| classification of agkistrodon species in china. | the wide geographical distribution of agkistrodon and the slight morphological differences among the snakes of the genus agkistrodon in china have posed a problem to taxonomists. we have employed polyacrylamide gel electrophoresis and immunological diffusion techniques for comparison of the venoms of different species and subspecies of agkistrodon from various localities. the electrophoretic patterns of the proteins of the venoms were different from each other, but showed certain relations withi ... | 1984 | 6426095 |
| mechanism of action of the platelet aggregation inhibitor purified from agkistrodon halys (mamushi) snake venom. | the platelet aggregation inhibitor purified from agkistrodon halys snake venom inhibited rabbit platelet aggregations induced by thrombin, sodium arachidonate, collagen or ionophore a-23187. the ic50 was about 11 micrograms/ml in platelet aggregation regardless of which aggregation inducer was used. beta-mercaptoethanol abolished both the phospholipase a enzymatic and platelet aggregation inhibitory activities of this venom inhibitor. p-bromophenacyl bromide-treated venom inhibitor lost almost c ... | 1984 | 6427979 |
| studies on phospholipase a inhibitor in blood plasma. i. purification and characterization of phospholipase a inhibitor in bovine plasma. | phospholipase a inhibitor was found in bovine, human and porcine plasma. the inhibitor was purified about 300-fold from bovine plasma by ammonium sulfate fractionation, deae-cellulose chromatography, sephadex g-200 gel filtration and anti-bsa antibody-agarose column chromatography to remove albumin. the purified material was homogeneous as judged by immunoelectrophoresis and 0.7% agarose-2% polyacrylamide gel electrophoresis. the inhibitor suppressed not only rat and human plasma phospholipase a ... | 1984 | 6501263 |
| purification of a capillary permeability increasing-enzyme from the venom of agkistrodon caliginosus (kankoku-mamushi). | a capillary permeability increasing-enzyme was purified from the venom of agkistrodon caliginosus by gel-filtration on sephadex g-100, ion exchange chromatographies on cm-sephadex c-50 and deae-sephadex a-50 and affinity chromatography on p-aminobenzamidine-epsilon-aminocaproic acid-sepharose 4b. by this procedure, 6.2 mg of the purified enzyme was obtained from 4 g of the venom. the purified enzyme was homogeneous by disc electrophoresis at ph 8.3 and ph 4.5, and did not show any caseinolytic, ... | 1983 | 6658808 |
| purification of two thrombin-like enzymes from the venom of agkistrodon caliginosus (kankoku-mamushi). | two thrombin-like enzymes were purified from the venom of agkistrodon caliginosus. both were homogeneous on polyacrylamide gel electrophoresis, hydrolyzed n-alpha-tosyl-l-arginine methylester and did not show any caseinolytic activity. the molecular weights of the enzymes were estimated to be about 34,000 - 37,000 and 42,000 - 43,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and by gel-filtration on sephadex g-100 column. one enzymes was purified from the venom by gel-filtrati ... | 1984 | 6719476 |
| the role of phospholipase a2 lysines in phospholipolysis of escherichia coli killed by a membrane-active neutrophil protein. | purified rabbit bactericidal/permeability-increasing protein at bactericidal concentrations is a membrane-perturbing agent that triggers hydrolysis of envelope phospholipids of a phospholipase a-less escherichia coli (s17) mutant by a highly basic (pi greater than 10) phospholipase a2, purified from agkistrodon halys blomhoffii snake venom. most other purified phospholipases a2 do not degrade the phospholipids of e. coli killed by the bactericidal protein. to study the role of enzyme charge in b ... | 1982 | 6815187 |
| identification of domains of phosphatidylcholine in human erythrocyte plasma membranes. differential action of acidic and basic phospholipases a2 from agkistrodon halys blomhoffii. | highly purified acidic (pi 4.9) and basic (pi 8.7) phospholipases a2 from snake (agkistrodon halys blomhoffii) venom hydrolyzed approximately 20% and 60%, respectively, of the phosphatidylcholine (pc) of intact human erythrocytes prior to hemolysis. sequential use of the acidic enzyme followed by the basic phospholipase a2 or vice versa manifested a characteristic pc hydrolysis pattern. for example, when acidic enzyme had hydrolyzed nearly 20% of this substrate, a subsequent treatment with the b ... | 1982 | 7061456 |
| topological location and biological significance of phospholipids in the membrane of newcastle disease virus. hydrolysis of phospholipids in intact virion with pure phospholipases a2, c, and d. | the composition, topological distribution and biological significance of phospholipids in the membrane of newcastle disease virus (ndv) grown in embryonated chicken eggs were investigated. phosphatidylethanolamine and sphingomyelin were the predominant phospholipids in ndv membrane. the location of phospholipids in the lipid bilayer of the membrane was studied by assessing their reactivities with highly purified phospholipase a2 (agkistrodon halys blomhoffi) and phospholipase d (streptomyces chr ... | 1982 | 7130158 |
| [isolation of phospholipase a2 on biospecific supports]. | the procedure of isolation of phospholipase a2 from agkistrodon halys blomhoffii venom and porcine pancreas on biospecific supports of an organo-silica type with immobilized phospholipid is described. the purity of isolated enzymes was controlled by polyacrylamide gel electrophoresis, determination of the n-terminal amino acid and isoelectrofocusing. the enzyme activity was equal to 80 mumole/mg/min and 1400 mumole/mg/min for snake venom phospholipase and for pancreatic phospholipase a2, respect ... | 1982 | 7138970 |
| the isolation and characterization of a third or neutral phospholipase a2 in the venom of agkistrodon halys blomhoffii. an improved fractionation procedure for all three enzymes. | the isolation of a new, third phospholipase a2 from agkistrodon halys blomhoffi is described. on the basis of a pi value of 6.9, it is termed a neutral phospholipase a2. it was characterized as to its amino acid content, activity towards phosphatidylcholine, heat stability, and hemolytic behavior on human erythrocytes. a comparison of these characteristics with those of the acidic and basic phospholipases a2 established the uniqueness of the neutral enzyme. two particularly important observation ... | 1980 | 7459370 |
| comparative study of fibrinogen degradation by four arginine ester hydrolases from the venom of agkistrodon caliginosus (kankoku-mamushi). | when purified capillary permeability-increasing (cpi)-enzyme-1, cpi-enzyme-2, kininogenase-1 or kininogenase-2 was incubated with human fibrinogen at a ratio of 1:100 by weight, a loss of fibrinogen coagulability was seen at prolonged incubation times. cpi-enzyme-2 and kininogenase-1 caused a rapid loss of coagulability, while cpi-enzyme-1 and kininogenase-2 acted more slowly. when human fibrinogen and each enzyme were incubated at 37 degrees c, cpi-enzyme-2 first cleaved the a alpha-chain then ... | 1995 | 7597721 |
| chemical modification and inactivation of phospholipases a2 by a manoalide analogue. | chemical modification and inactivation of bovine pancreatic, porcine pancreatic, naja naja atra and pseudechis australis phospholipases a2 (pla2s), belonging to group i, and of trimeresurus flavoviridis, vipera russelli russelli and agkistrodon halys blomhoffii pla2s, belonging to group ii, were investigated by the use of a manoalide (mld)-analogue, 1-(2,5-dihydro-hydroxy-5-oxo-3-furanyl)-8,12-dimethyl-4-formyl-3,7, 11-tridecatrienol. at appropriate time intervals, residual pla2 activities towar ... | 1995 | 7755577 |
| binding mode of phospholipase a2 with a new type of phospholipid analog having an oxazolidinone ring. | inhibition of phospholipases a2 (pla2s) by a new type of monodispersed phospholipid analog, 3-dodecanoyl-4-phosphatidylcholinohydroxymethyl-2-oxazolidinone (oxazolidinone-pc), was investigated by the ph stat assay method using monodispersed 1,2-dihexanoyl-sn-glycero-3-phosphorylcholine (dic6pc) as the substrate. the pla2s used were those from bovine pancreas and cobra (naja naja atra) venom (group i) and from japanese mamushi (agkistrodon halys blomhoffii) venom (group ii). this new-type substra ... | 1995 | 7775386 |
| role of ca2+ in the binding of phospholipase a2 with a monomeric substrate and with its amide-type analog. | effects of ca2+ on the kinetic parameters for the hydrolysis of monodispersed 1,2-dihexanoyl-sn-glycero-3-phosphorylcholine (dic6pc), catalyzed by group i phospholipases a2 (pla2s) from pseudechis australis, naja naja atra, and bovine pancreas and by group ii enzymes from vipera russelli russelli, agkistrodon halys blomhoffii, and trimeresurus flavoviridis, were studied by the ph-stat assay method at 25 degrees c, ph 7.5-8.2, and an ionic strength of 0.1 or 0.2 in the absence or presence of an a ... | 1994 | 7883763 |
| positional distribution of octadecadienoic acids in sponge phosphatidylethanolamines. | the distribution of c14-c22 fatty acids in the phosphatidylethanolamines isolated from the sponges agelas sp. and spongia tampa was investigated. selective changes with phospholipase a2 (from agkistrodon halys blomhoffii) followed by thin-layer chromatographic separation of the resulting lysophosphatidylethanolamines and free fatty acids and subsequent methylation with hcl/meoh and diazomethane, respectively, revealed that the 5,9-octadecadienoic acid and the 9,12-octadecadienoic acid present sh ... | 1994 | 7968275 |
| inhibition of hemorrhagic activities of various snake venoms by purified antihemorrhagic factor obtained from japanese habu snake. | the ability of purified antihemorrhagic factor isolated from the serum of japanese habu (trimeresurus flavoviridis) was tested on 17 snake venoms to inhibit their hemorrhagic activity. the factor strongly inhibited that of the venoms of crotalus horridus horridus and vipera latastei gaditana in addition to that of the homologous (t. flavoviridis) venom. hemorrhagic activity of agkistrodon halys blomhoffi, agkistrodon contortrix contortrix, bothrops atrox asper and crotalus atrox venoms was also ... | 1994 | 8016857 |
| [the antithrombotic action of a protein c activator from the venom of agkistrodon blomhoffi ussuriensis in thrombus formation in an extracorporeal shunt in rats]. | an antithrombotic action of the protein c (pc) activator from the venom of agkistrodon blomhoffi ussuriensis on the model of platelet-dependent thrombosis in the arteriovenous shunt in rats was under investigation. administration of the pc activator to rats resulted in a dose-dependent prolongation of the thrombus formation time, in a decrease in pc and factor v levels in blood and in aptt prolongation. there were no changes in the tissue-type plasminogen activator level and in the adp- or epine ... | 1994 | 8205048 |
| purification of a capillary permeability-increasing enzyme-2 from the venom of agkistrodon caliginosus (kankoku-mamushi). | a capillary permeability-increasing enzyme-2 was purified from the venom of a. caliginosus by ion-exchange chromatography and gel filtration on sephadex g-100. by this procedure, 3.1 mg of purified enzyme was obtained from 4 g of the venom. the mol. wt of the purified enzyme was estimated to be approximately 44,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the enzyme hydrolyzed n-alpha-tosyl-l-arginine methylester with a specific activity of 56.5 units/mg of protein, and did ... | 1993 | 8303715 |
| protein c activator from the venom of agkistrodon blomhoffi ussuriensis retards thrombus formation in the arterio-venous shunt in rats. | protein c (pc) is an anticoagulant protein which, being activated by thrombin, degrades factors v/va and viii/viiia and releases a tissue-type plasminogen activator. some agkistrodon snake venoms contain pc activators which, in experiments, exert an anticoagulant action. an antithrombotic effect of the pc activator from the venom of a. blomhoffi ussuriensis on the model of thrombus formation in the arterio-venous shunt in rats was under investigation. administration of the pc activator resulted ... | 1993 | 8378894 |
| hydrolysis of micellar diheptanoylphosphatidylcholine catalyzed by bovine pancreatic phospholipase a2: kinetic characterization of group i and ii enzymes. | initial velocity data for the hydrolysis of micellar 1,2-diheptanoyl-sn-glycero-3-phosphorylcholine (dic7pc) catalyzed by bovine pancreatic pla2 (group i) were analyzed using the michaelis-menten equation. the km value for the micellar substrate was found to be independent of ca2+ concentration, as was the km value for the monodispersed substrate. the ph dependence curve of km in the presence of saturating amounts of ca2+ showed two transitions reflecting large pk shifts of two ionizable groups ... | 1993 | 8454568 |
| isolation and amino acid sequence of a phospholipase a2 inhibitor from the blood plasma of agkistrodon blomhoffii siniticus. | phospholipase a2 inhibitor (pli) was purified from the blood plasma of chinese mamushi, agkistrodon blomhoffii siniticus, by sequential chromatography on sephadex g-200, mono q, and blue-sepharose cl-6b columns. the purified pli was a glycoprotein with an apparent molecular mass of 75 kda and was composed of a single subunit with a mass of about 20 kda. from the results of a cross-linking experiment, the pli was found to present as a homotrimer of the subunit. the fundamental properties of a. bl ... | 1993 | 8514730 |
| pharmacological study on agkistrodon blomhoffii blomhoffii boie. v. anti-fatigue effect of the 50% ethanol extract in acute weight-loaded forced swimming-treated rats. | the anti-fatigue effect of 50% ethanol extract ([m]) from the dried whole body of agkistrodon blomhoffii blomhoffii boie, was investigated using an acute weight-loaded forced swimming (awlfs) test by monitoring swimming times, blood biochemical parameters, thiobarbiturate-reactive substances (tbars) as an index of lipid peroxide and antioxidative enzyme activities in blood and tissue. [m] (500 mg/kg/d), given orally for three successive days, significantly prolonged swimming times. it also inhib ... | 1996 | 8820913 |
| inhibition of angiotensin converting enzyme and potentiation of bradykinin by retro-inverso analogues of short peptides and sequences related to angiotensin i and bradykinin. | there is pharmacological evidence indicating that, in addition to the inhibition of angiotensin converting enzyme (ace; ec 3.4.15.1), the potentiation of bradykinin (bk) responses may also involve the bk receptor or some binding site in the structures involved in the contractile response to this peptide. dipeptides such as val-trp and some of its analogues as well as tripeptide homologues, including total and partial retro-inverso peptides, were synthesized and assayed for their ability to inhib ... | 1996 | 8866827 |
| effect of extract of agkistrodon blomhoffii on acute gastric mucosal lesions induced by ischaemia-reperfusion in rats. | | 1997 | 9055201 |
| capillary permeability-increasing enzyme-2 from the venom of agkistrodon caliginosus (kankoku-mamushi): activity resulting from the release of peptides from fibrinogen. | when a mixture of the purified capillary permeability-increasing enzyme-2 from agkistrodon caliginosus venom and rabbit plasma was injected into the depilated skin of the back of rabbits, the capillary permeability-increasing activity was much greater than that induced by injection of the enzyme alone. to isolate the active substance, the mixture was subjected to reversed-phase high-performance liquid chromatography. among the active peptides, five peptides were purified and their amino acid seq ... | 1997 | 9133714 |
| biochemical characterization and pharmacological properties of a phospholipase a2 myotoxin inhibitor from the plasma of the snake bothrops asper. | a protein that neutralizes the biological activities of basic phospholipase a2 (pla2) myotoxin isoforms from the venom of the snake bothrops asper was isolated from its blood by affinity chromatography with sepharose-immobilized myotoxins. biochemical characterization of this b. asper myotoxin inhibitor protein (bamip) indicated a subunit molecular mass of 23-25 kda, an isoelectric point of 4, and glycosylation. gel-filtration studies revealed a molecular mass of 120 kda, suggesting that bamip p ... | 1997 | 9307037 |
| purification and amino acid sequence of halystase from snake venom of agkistrodon halys blomhoffii, a serine protease that cleaves specifically fibrinogen and kininogen. | we have isolated a serine protease, halystase, from agkistrodon halys blomhoffii venom by chromatography on deae-sepharose, heparin-sepharose and q-sepharose columns, and have determined the complete amino acid sequence by edman degradation and by mass spectral analysis of peptides generated by enzymatic and chemical cleavage. the 238-residue sequence of halystase, containing n-linked carbohydrates (about 13%) at two sites showed significant similarity to other thrombin-like snake venom serine p ... | 1998 | 9546675 |
| a novel phospholipase a2 inhibitor with leucine-rich repeats from the blood plasma of agkistrodon blomhoffii siniticus. sequence homologies with human leucine-rich alpha2-glycoprotein. | the phospholipase a2 (pla2) inhibitor plibeta, purified from the blood plasma of chinese mamushi snake (agkistrodon blomhoffii siniticus), is a 160-kda trimer with three 50-kda subunits; and it inhibits specifically the enzymatic activity of the basic pla2 from its own venom (ohkura, n., okuhara, h., inoue, s., ikeda, k., and hayashi, k. (1997) biochem. j. 325, 527-531). in the present study, the 50-kda subunit was found to be glycosylated with n-linked carbohydrate, and enzymatic deglycosylatio ... | 1998 | 9677367 |
| blomhotin: a novel peptide with smooth muscle contractile activity identified in the venom of agkistrodon halys blomhoffii. | a novel peptide has been isolated from the venom of agkistrodon halys blomhoffii using a bioassay that monitors the stimulant effect on rat stomach fundus. the 11-amino acid peptide, named blomhotin, was purified to homogeneity by gel-filtration column chromatography and reverse-phase hplc. the amino acid sequence of blomhotin was determined to be pglu-gly-arg-pro-pro-gly-pro-pro-ile-pro-arg, which is similar to that of bradykinin-potentiating peptides which themselves cause no contraction of sm ... | 1999 | 10519653 |
| cdna cloning and bacterial expression of phospholipase a(2) inhibitor plialpha from the serum of the chinese mamushi, agkistrodon blomhoffii siniticus(1). | the cdna encoding of a phospholipase a(2) inhibitor (plialpha) of the chinese mamushi, agkistrodon blomhoffii siniticus, was identified from a liver cdna library by use of a probe prepared by polymerase chain reaction (pcr) on the basis of the amino acid sequence of plialpha. it encoded a polypeptide of 166 amino acid residues, including 19 residues of the signal sequence and 147 residues of the complete mature sequence of plialpha. the plialpha cdna was subcloned into the expression vector pet- ... | 1999 | 10526227 |
| beta-agkistrodotoxin inhibits fast and ca2+-activated k+ currents recorded from mouse motor nerve terminals. | beta-agkistrodotoxin (beta-agtx), a polypeptide purified from the venom of agkistrodon blomhoffii brevicaudus, is a presynaptic blocker acting on neurotransmitter release. in this work, perineural recording technique was employed to study the effects of beta-agtx on sodium, potassium and calcium currents of mouse motor nerve terminals. the results showed that beta-agtx selectively inhibited ca2+-dependent (i(k,ca)) and fast (i(k,f) k+ currents, but did not affect slow k+ current (i(k,s)), sodium ... | 2000 | 10665799 |
| cdna cloning of bradykinin-potentiating peptides-c-type natriuretic peptide precursor, and characterization of the novel peptide leu3-blomhotin from the venom of agkistrodon blomhoffi. | a cdna clone, 1.8 kb long, was isolated from a venom gland cdna library of agkistrodon blomhoffi that encodes a large plurifunctional precursor composed of 263 amino-acid residues. nucleotide sequence analysis of this clone revealed that sequences which code for blomhotin and a novel peptide leu3-blomhotin are located in the n-terminal region of the precursor polypeptide, followed by four tandemly aligned sequences which code for three types of bradykinin-potentiating peptide. in the c-terminal ... | 2000 | 10866809 |
| structure-activity studies of analogues of blomhotin mediating contraction of rat fundus. | blomhotin is a novel peptide (pglu1-gly2-arg3-pro4-pro5-gly6-pro7-pro8-ile9-pro10-arg11) which has been isolated from the venom of agkistrodon halys blomhoffii and exhibits contractile activity on rat stomach fundus. we carried out a structure-activity study of blomhotin and its related peptides, and the findings suggested that the n-terminal portion of blomhotin is mainly responsible for affinity for the blomhotin receptor, whereas the c-terminal portion of blomhotin, pro-ile-pro-arg, is respon ... | 2000 | 11085371 |
| conservation of k1 immunoreactivity against early cortical neurones in the vertebrate telencephalon. | a monoclonal antibody, k1, immunostains neurones generated earliest in the rat neocortex. the k1 immunoreactivity was found in both mouse and human embryos. in the human marginal zone, the subpial granular layer and the inner sublayer were stained at the 19th and 20th week of gestation, respectively. western blot analysis revealed that the k1 immunoreactivity was conserved in a variety of vertebrates. while a protein of low molecular weight (200 kda) reacted dominantly in an amphibian (xenopus l ... | 2001 | 11164259 |
| cloning and characterization of novel snake venom proteins that block smooth muscle contraction. | in this study, we isolated a 25-kda novel snake venom protein, designated ablomin, from the venom of the japanese mamushi snake (agkistrodon blomhoffi). the amino-acid sequence of this protein was determined by peptide sequencing and cdna cloning. the deduced sequence showed high similarity to helothermine from the mexican beaded lizard (heloderma horridum horridum), which blocks voltage-gated calcium and potassium channels, and ryanodine receptors. ablomin blocked contraction of rat tail arteri ... | 2002 | 12047379 |
| case report: extraocular muscle paresis caused by snakebite. | a 51 year-old man presented with binocular diplopia on the three days after the snakebite in the fifth finger of the right hand by an agkistrodon blomhoffi (mamushi). in the primary position he had an exotropia and right hypertropia, which became apparent when his head was tilted to the right. from ocular angle of deviation measured by synoptophore and hess chart test, he was diagnosed as having medial rectus muscle paresis as well as inferior oblique muscle paresis of the left eye. elevation de ... | 2003 | 12698017 |
| anticoagulant activity of m-lao, l-amino acid oxidase purified from agkistrodon halys blomhoffii, through selective inhibition of factor ix. | one of haemorrhagic toxins present in snake venoms is l-amino acid oxidase (lao), which catalyzes the oxidative deamination of l-amino acids with the generation of hydrogen peroxide. although it is widely accepted that lao alters platelet function, the effects of lao on human blood coagulation remain largely unknown. the present study demonstrated, for the first time, that m-lao, lao purified from the venom of agkistrodon halys blomhoffii (japanese mamushi), possesses an anticoagulant activity. ... | 2003 | 12818190 |
| relapse or reinfection in tetanus and diphtheria, and reenvenomation in mamushi and habu snake bites. problems associated with reinjection of horse serum antitoxin or antivenom in man. | | 1964 | 14192591 |
| the relationship between the hemorrhagic and lethal activities of japanese mamushi (agkistrodon halys blomhoffii) venom. | | 1964 | 14302275 |
| purification and cloning of cysteine-rich proteins from trimeresurus jerdonii and naja atra venoms. | three 26 kda proteins, named as tj-crvp, na-crvp1 and na-crvp2, were isolated from the venoms of trimeresurus jerdonii and naja atra, respectively. the n-terminal sequences of tj-crvp and na-crvps were determined. these components were devoid of the enzymatic activities tested, such as phospholipase a(2), arginine esterase, proteolysis, l-amino acid oxidase, 5'nucleotidase, acetylcholinesterase. furthermore, these three components did not have the following biological activities: coagulant and a ... | 2003 | 14529736 |
| effect of agkistroden blomhoffi (mamushi) on the proliferation of human fibroblasts. | the purpose of this study is to investigate the effect of agkistroden blomhoffi (mamushi) aqueous extract on human patellar tendon cells in vitro, to pharmacologically explain the natural medicine's healing effect on tendon, bone and muscle injuries. | 2004 | 14725945 |
| inhibitory effects on phospholipase a2 and antivenin activity of melanin extracted from thea sinensis linn. | antivenin activity of melanin extracted from black tea (mebt) was reported for the first time. the antagonistic effect of mebt was evaluated for agkistrodon contortrix laticinctus (broadbanded copperhead), agkistrodon halys blomhoffii (japanese mamushi), and crotalus atrox (western diamondback rattlesnake) snake venoms administered i.p. to icr mice. mebt was injected i.p. immediately after the venom administration in dose of 3 mg per mouse in the same place of venom injection. mebt demonstrated ... | 2004 | 14967198 |
| [purification and characteristics of polypeptide with inhibition of platelet aggregation from agkistrodon blomhoffii bioe]. | a polypeptide, sv-pp-2, was purified from agkistrodon blomhoffii bioe by hplc c18 column. the sv-pp-2 has molecular weight of 23339.00 da, and can inhibit the adp-induced platelet aggregation in dose-dependent manner. there have been no such polypeptides reported yet which has inhibition of platelet aggregation and similar molecular weight to sv-pp-2. | 2004 | 15807242 |
| [purification of small peptide from agkistrodon blomhoffii bioe and its inhibition on platelet aggregation]. | this is the first report to purify a new peptide sv-pp-1 from agkistrodon blomhoffii bioe. sv-pp-1 has molecular weight of 1234.616 da and can inhibit on platelet aggregation. sv-pp-1 significantly inhibited the adp-induced platelet aggregation. it has been found up to now that sv-pp-1 is the smallest peptide inhibiting on platelet aggregation from snake venom. | 2004 | 15810593 |
| actin-inhibition and folding of vertebrate deoxyribonuclease i are affected by mutations at residues 67 and 114. | amino acid (aa) residues (val-67 and ala-114) have been suggested as being mainly responsible for actin-binding in human and bovine deoxyribonucleases i (dnase i). this study presents evidence of these two aa mutational mechanisms, not only for actin-binding but also for folding of dnase i in mammals, reptiles and amphibians. human and viper snake (agkistrodon blomhoffii) enzymes are inhibited by actin, whereas porcine, rat snake (elaphe quadrivirgata), and african clawed frog (xenopus laevis) e ... | 2006 | 16311052 |
| [purification of a new phospholipase a2 homologue from agkistrodon blomhoffii siniticus and its effects on gene expression profile of hep3b cells]. | to isolate and purify a new phospholipase a2 (pla2) homologue from agkistrodon blomhoffii siniticus and investigate its effects on the gene expression profile of hep3b cells. | 2006 | 16495181 |
| standardization of regional reference for mamushi (gloydius blomhoffii) antivenom in japan, korea, and china. | the mamushi (gloydius blomhoffii) snakes that inhabit japan, korea, and china produce venoms with similar serological characters to each other. individual domestic standard mamushi antivenoms have been used for national quality control (potency testing) of mamushi antivenom products in these countries, because of the lack of an international standard material authorized by the world health organization. this precludes comparison of the results of product potency testing among countries. we estab ... | 2006 | 16495629 |
| effect of agkistroden blomhoffi (mamushi) on superoxide generation in human neutrophils. | the purpose of this study is to investigate the effect of agkistroden blomhoffi (mamushi) aqueous extract on stimulus-induced superoxide generation in human neutrophils. | 2006 | 16714011 |
| a novel peptide from the acei/bpp-cnp precursor in the venom of crotalus durissus collilineatus. | in crotaline venoms, angiotensin-converting enzyme inhibitors [aceis, also known as bradykinin potentiating peptides (bpps)], are products of a gene coding for an acei/bpp-c-type natriuretic peptide (cnp) precursor. in the genes from bothrops jararaca and gloydius blomhoffii, acei/bpp sequences are repeated. sequencing of a cdna clone from venom glands of crotalus durissus collilineatus showed that two aceis/bpps are located together at the n-terminus, but without repeats. an additional sequence ... | 2006 | 16979945 |
| [characterization of platelets aggregation inhibitor from agkistrodon blomhoffii ussuriensis snake venom]. | platelet aggregation inhibitor--"blomus-b" from agkistrodon blomhoffii ussuriensis venom has been isolated by affinity and ion-exchange chromatography. the purified inhibitor is a novel non-enzymatic single-chain protein with molecular weigth of 13 kda. "blomus-b" causes a change of platelets shape and takes effect on adp- and adrenalin-induced platelet aggregation. | 2006 | 17100321 |
| purification, characterization and potent lung lesion activity of an l-amino acid oxidase from agkistrodon blomhoffii ussurensis snake venom. | l-amino acid oxidases (laos) are one of the major components of snake venoms, which possess numerous biological functions. however, little is known of the influence of laos on organ lesions. in the present study, a unique lao from agkistrodon blomhoffii ussurensis snake venom named abu-lao was purified by heparin-sepharose ff chromatography followed by an ion-exchange chromatography procedure. the purified abu-lao appears a dimer with a molecular mass of approximately 108.8kda. kinetics studies ... | 2007 | 17854853 |
| properties and cdna cloning of antihemorrhagic factors in sera of chinese and japanese mamushi (gloydius blomhoffi). | an antihemorrhagic protein has been isolated from the serum of chinese mamushi (gloydius blomhoffi brevicaudus) by using a combination of ethanol precipitation and a reverse-phase high-performance liquid chromatography (hplc) on a c8 column. this protein-designated chinese mamushi serum factor (cmsf)-suppressed mamushi venom-induced hemorrhage in a dose-dependent manner. it had no effect on trypsin, chymotrypsin, thermolysin, and papain but inhibited the proteinase activities of several snake ve ... | 2008 | 18037153 |
| amino acid sequence of a kinin-releasing enzyme, kr-e-1, from the venom of agkistrodon caliginosus (kankoku-mamushi). | the amino acid sequence of a bradykinin-releasing enzyme, named kr-e-1, isolated from the venom of agkistrodon caliginosus (kankoku-mamushi) was determined by edman sequencing of the peptides which was derived from digests with cyanogen bromide, hydroxylamine, achromobacter protease i, trypsin, v8 protease, arginine endopeptidase, and endoproteinase asp-n. kr-e-1 consisted of 235 amino acids and showed conservation of the catalytic amino acid residues (his(57), asp(102), and ser(195)) of the chy ... | 2008 | 18723043 |
| rapid evolution in the wild: changes in body size, life-history traits, and behavior in hunted populations of the japanese mamushi snake. | rapid evolution caused by human exploitation of wildlife is not usually addressed in studies of the impacts of such exploitation despite its direct relevance to population persistence. japanese mamushi (gloydius blomhoffii), an endemic venomous snake of the japanese archipelago, has been heavily hunted by humans, and many populations appear to be declining or are already extirpated. we compared local populations that have been hunted regularly with populations that have not been hunted. mamushi ... | 2009 | 18798855 |
| snake fetuin: isolation and structural analysis of new fetuin family proteins from the sera of venomous snakes. | novel proteins were isolated from the sera of chinese mamushi (gloydius blomhoffi brevicaudus) and habu (trimeresurus flavoviridis). the primary structures of these proteins were determined by protein sequencing, and the nucleotide sequences were established by cdna cloning from the liver mrnas. they belonged to the fetuin family having a double-headed cystatin-like domain and a his-rich domain, akin to hsf, an antihemorrhagic factor isolated from habu serum. they showed no antihemorrhagic activ ... | 2009 | 19481564 |
| biochemical, functional and structural characterization of akbu-laao: a novel snake venom l-amino acid oxidase from agkistrodon blomhoffii ussurensis. | an l-amino acid oxidase (akbu-laao) was isolated from the venom of agkistrodon blomhoffii ussurensis snake using deae sephadex a-50 ion-exchange, sephadex g-75 gel filtration, and high performance liquid chromatographies. the homogeneity and molecular mass of akbu-laao were analyzed by sds-page and maldi-tof spectrometry. the sequences of ten peptides from akbu-laao were established by hplc-nesi-ms/ms analysis. protein sequence alignment indicated that i) that akbu-laao is a new snake venom laao ... | 2010 | 20100538 |
| [binocular diplopia and ptosis due to snakebite (agkistrodon blomhoffi "mamushi")--a case report]. | we treated a patient who developed binocular diplopia and ptosis after being bitten by an agkistrodon blomhoffi (mamushi). the patient was a 49-year-old man who presented with binocular diplopia after the snakebite on the second finger of his right hand. he experienced local pain and swelling and a few hours later, he developed diplopia. in the primary position he had no tropia. on the basis the ocular angle of deviation measured by a hess chart test, he was diagnosed with paresis of the medial ... | 2010 | 20297733 |
| application of mass spectrometry to sequence analysis of pyroglutamyl peptides from snake venoms: contribution to the confirmation of the amino acid sequence of bradykinin-potentiating peptides b, c and e isolated from the venom of agkistrodon halys blomhoffii. | | 2016 | 4775967 |
| interaction of monodispersed and micellar phospholipids with an agkistrodon halys blomhoffii phospholipase a2, in which the alpha-amino group had been modified to an alpha-keto group. | the ph dependence of the binding constant of ca2+ to a phospholipase a2 of agkistrodon halys blomhoffii, in which the alpha-amino group had been selectively modified to an alpha-keto group, was studied at 25 degrees c and ionic strength 0.1 by the tryptophyl fluorescence method. the dependence was compared with the results for the intact enzyme (ikeda et al. (1981) j. biochem. 90, 1125-1130). the ph-dependence curve could be well interpreted in terms of the participation of the two ionizable gro ... | 2014 | 3957900 |
| the fate of proteinases of mamushi venom during preparation of toxoid. | | 2015 | 4596709 |
| venomous snake bites: clinical diagnosis and treatment. | snake bites are life-threatening injuries that can require intensive care. the diagnosis and treatment of venomous snake bites is sometimes difficult for clinicians because sufficient information has not been provided in clinical practice. here we review the literature to present the proper management of bites by mamushi, habu, and yamakagashi snakes, which widely inhabit japan and other asian countries. no definite diagnostic markers or kits are available for clinical practice; therefore, defin ... | 2015 | 25866646 |
| structure of a basic phospholipase a2 from agkistrodon halys pallas at 2.13 a resolution. | the basic phospholipase a2 isolated from the venom of agkistrodon halys pallas (agkistrodon blomhoffii brevicaudus) is a hemolytic toxin and one of the few pla2's capable of hydrolyzing the phospholipids of e. coli membranes in the presence of a bactericidal/permeability-increasing protein (bpi) of neutrophils. the crystal structure has been determined and refined at 2.13 a to an r factor of 16.5% (f > 3sigma) with excellent stereochemistry. a superposition of the two molecules in the asymmetric ... | 1998 | 9761847 |
| cloning of cdnas encoding c-type lectins from elapidae snakes bungarus fasciatus and bungarus multicinctus. | a number of c-type lectins with various biological activities have been purified and characterized from viperidae snake venoms. in contrast, only a few reports could be found in literature concerning the c-type lectins in elapidae snake venoms. based on the published cdna sequences of c-type lectins from viperidae snake venoms, oligonucleotide primers were designed and used to screen the cdna libraries made from the venom glands of bungarus fasciatus and bungarus multicinctus. this allowed the c ... | 2001 | 11600152 |
| presence of heat-stable hemorrhagic toxins in snake venoms. | twenty-eight snake venoms (seven agkistrodon venoms, six bothrops venoms, 13 crotalus venoms, one sistrurus venom, and one bitis venom) were examined for the presence of heat-stable (100 degrees c, 5 min) hemorrhagic toxins. both heated and unheated venoms were analyzed for their protein composition by sds-page, and tested for their hemorrhagic activity in vivo in mice and for their proteolytic activity on two different substrates. heating all venoms led to the denaturation and loss of some prot ... | 1994 | 7985199 |
| isolation and amino acid sequence of a phospholipase a2 inhibitor from the blood plasma of the sea krait, laticauda semifasciata. | a phospholipase a2 (pla2) inhibitor was purified from the blood plasma of a sea krait, laticauda semifasciata, by sequential chromatography on sephadex g-200, mono q, and phenyl sepharose columns. the purified inhibitor was found to be the same type as the pla2 inhibitors, named pligamma, that had been purified from the blood plasma of the thai cobra naja naja kaouthia [ohkura et al. (1994) biochem. biophys. res. commun. 200, 784-788] and chinese mamushi agkistrodon blomhoffii siniticus [ohkura ... | 1999 | 9990137 |
| purification and characterization of three distinct types of phospholipase a2 inhibitors from the blood plasma of the chinese mamushi, agkistrodon blomhoffii siniticus. | three distinct types of phospholipase a2 (pla2) inhibitory proteins (plialpha, plibeta, and pligamma) were isolated from the blood plasma of the chinese mamushi, agkistrodon blomhoffii siniticus. plialpha is an inhibitor that we have already purified and whose amino acid sequence we have already determined [ohkura, inoue, ikeda and hayashi (1993) j. biochem. (tokyo) 113, 413-419]. it inhibited selectively the group-ii acidic pla2s from crotalidae venom. plibeta was a 160-kda glycoprotein having ... | 1997 | 9230137 |
| rosmarinic acid in argusia argentea inhibits snake venom-induced hemorrhage. | a methanolic extract of argusia (or messerschmidia or tournefortia) argentea (boraginaceae) significantly inhibited hemorrhage induced by crude venom of trimeresurus flavoviridis. the extract was then separated according to antivenom activity by using silica gel column chromatography and hplc equipped with an octadecylsilanized silica gel (ods) column to afford rosmarinic acid (ra) (1) as an active principle. ra (1) significantly inhibited the hemorrhagic effect of crude venoms of t. flavoviridi ... | 2010 | 20512530 |
| muscle extract of hedgehog, erinaceus europaeus, inhibits hemorrhagic activity of snake venoms. | the antihemorrhagic activity of muscle extract of hedgehog, erinaceus europaeus, was tested on various snake venoms with hemorrhagic activity. the extract inhibited strongly hemorrhagic activity of venoms from bitis arietans, bothrops jararaca and vipera latastei gaditana, and remarkably that of venoms from agkistrodon halys blomhoffi, bitis gabonica rhinoceros, bitis nasicornis, bothrops atrox asper, crotalus horridus horridus and vipera berus. the antihemorrhagic activity against eight other s ... | 1994 | 7846699 |
| molecular cloning and characterization of satellite dna sequences from constitutive heterochromatin of the habu snake (protobothrops flavoviridis, viperidae) and the burmese python (python bivittatus, pythonidae). | highly repetitive dna sequences of the centromeric heterochromatin provide valuable molecular cytogenetic markers for the investigation of genomic compartmentalization in the macrochromosomes and microchromosomes of sauropsids. here, the relationship between centromeric heterochromatin and karyotype evolution was examined using cloned repetitive dna sequences from two snake species, the habu snake (protobothrops flavoviridis, crotalinae, viperidae) and burmese python (python bivittatus, pythonid ... | 2015 | 26205503 |
| characterization and cdna cloning of aminopeptidase a from the venom of gloydius blomhoffi brevicaudus. | the aminopeptidase activities of snake venoms from gloydius blomhoffi brevicaudus, gloydius halys blomhoffii, trimeresurus flavoviridis, bothrops jararaca and crotalus atrox were investigated. aminopeptidase a (apa), aminopeptidase b and aminopeptidase n activities were present in all snake venoms. the strongest apa activity was found in venom from g. blomhoffi brevicaudus. the susceptibility to metallopeptidase inhibitors and the ph optimum of the partially purified enzyme from g. blomhoffi bre ... | 2007 | 17383704 |
| bradykinin-potentiating peptides and c-type natriuretic peptides from snake venom. | cloning of cdnas encoding bradykinin-potentiating peptides (bpps)-c-type natriuretic peptide (cnp) precursor or its homologue was performed for cdna libraries of bothrops jararaca (south american snake), trimeresurus flavoviridis, trimeresurus gramineus and agkistrodon halys blomhoffi (asian snakes), all belonging to crotalinae subfamily. each cdna library was constructed from the venom glands of a single snake to preclude ambiguity by intraspecies variation in venom components. thirteen positiv ... | 1999 | 10604536 |
| immunochemical analysis of a snake venom phospholipase a2 neurotoxin, crotoxin, with monoclonal antibodies. | crotoxin is the major neurotoxic component of the venom of the south american rattlesnake, crotalus durissus terrificus. the crotoxin molecule is composed of two subunits: a basic and weakly toxic phospholipase a2 (pla2) called component-b (cb), and an acidic, nonenzymatic and nontoxic subunit called component-a (ca). crotoxin exists as a mixture of several isoforms (or variants) resulting from the association of several subunit isoforms. we prepared monoclonal antibodies (mabs) against each iso ... | 2016 | 1378931 |
| a single amino acid substitution of leu130ile in snake dnases i contributes to the acquisition of thermal stability. | we purified pancreatic deoxyribonucleases i (dnases i) from three snakes, elaphe quadrivirgata, elaphe climacophora and agkistrodon blomhoffii, and cloned their cdnas. each mature snake dnase i protein comprised 262 amino acids. wild-type snake dnases i with leu130 were more thermally unstable than wild-type mammalian and avian dnases i with ile130. after substitution of leu130ile, the thermal stabilities of the snake enzymes were higher than those of their wild-type counterparts and similar to ... | 2003 | 12605681 |