| mesorhizobium septentrionale sp. nov. and mesorhizobium temperatum sp. nov., isolated from astragalus adsurgens growing in the northern regions of china. | ninety-five rhizobial strains isolated from astragalus adsurgens growing in the northern regions of china were classified into three main groups, candidate species i, ii and iii, based on a polyphasic approach. comparative analysis of full-length 16s rrna gene sequences of representative strains showed that candidate species i and ii were mesorhizobium, while candidate species iii, which consisted of non-nodulating strains, was closely related to agrobacterium tumefaciens. the phylogenetic relat ... | 2004 | 15545425 |
| different mesorhizobium species associated with caragana carry similar symbiotic genes and have common host ranges. | fourteen strains representing 11 caragana-nodulating mesorhizobium genomic species were identified as representing mesorhizobium amorphae, mesorhizobium huakuii, mesorhizobium septentrionale and groups related to mesorhizobium plurifarium, mesorhizobium temperatum, mesorhizobium tianshanense and mesorhizobium mediterraneum by sequencing of the 16s rrna gene, 16s-23s internal transcribed spacer, partial housekeeping reca gene, and previously performed sodium dodecyl sulfate polyacrylamide gel ele ... | 2008 | 18422620 |
| mesorhizobium caraganae sp. nov., a novel rhizobial species nodulated with caragana spp. in china. | five rhizobial strains representing the largest group in the genus mesorhizobium associated with caragana spp. in china were characterized taxonomically. phylogenetic analysis based on 16s rrna gene sequences indicated that these microsymbionts belonged to the genus mesorhizobium, with mesorhizobium tianshanense usda 3592(t), mesorhizobium temperatum sdw018(t) and mesorhizobium mediterraneum upm-ca36(t) as the closest neighbours (>/=99.5 % 16s rrna gene sequence similarity). genotypic fingerprin ... | 2008 | 18984708 |
| mesorhizobium robiniae sp. nov., isolated from root nodules of robinia pseudoacacia. | previously, five rhizobial strains isolated from root nodules of robinia pseudoacacia were assigned to the same genospecies on the basis of identical 16s rrna gene sequences and phylogenetic analyses of the noda, nodc and nifh genes, in which the five isolates formed a well-supported group that excluded other sequences found in public databases. in this study, the 16s rrna gene sequence similarities between the isolates and mesorhizobium mediterraneum upm-ca36(t) and mesorhizobium temperatum sdw ... | 2010 | 20008111 |
| evolution and taxonomy of native mesorhizobia nodulating medicinal glycyrrhiza species in china. | previously, 159 bacterial strains were isolated from the root nodules of wild perennial glycyrrhiza legume species grown on 40 sites in central and north-western china, in which 57 strains were classified as "true symbionts" belonging to the genus mesorhizobium based on amplified fragment length polymorphism (aflp) genomic fingerprinting and partial sequences of the 16s rrna gene [20]. in the present work, the phylogeny of glycyrrhiza nodulating mesorhizobia was further examined by multilocus se ... | 2016 | 27105685 |
| genetic diversity, community structure and distribution of rhizobia in the root nodules of caragana spp. from arid and semi-arid alkaline deserts, in the north of china. | the genetic diversity of 88 caragana nodule rhizobial isolates, collected from arid and semi-arid alkaline sandy soils in the north of china, was assessed by pcr-rflp of the 16s rrna gene and the 16s-23s igs, as well as the phylogenies of housekeeping genes (atpd, glnii and reca) and symbiotic genes (nodc and nifh). of the 88 strains, 69 were placed in the genus mesorhizobium, 16 in rhizobium and 3 in bradyrhizobium. mesorhizobium amorphae, mesorhizobium septentrionale, mesorhizobium temperatum ... | 2012 | 22463809 |
| genetic diversity and distribution of rhizobia associated with the medicinal legumes astragalus spp. and hedysarum polybotrys in agricultural soils. | with the increasing cultivation of medicinal legumes in agricultural fields, the rhizobia associated with these plants are facing new stresses, mainly from fertilization and irrigation. in this study, investigations on the nodulation of three cultivated medicinal legumes, astragalus mongholicus, astragalus membranaceus and hedysarum polybotrys were performed. bacterial isolates from root nodules of these legumes were subjected to genetic diversity and multilocus sequence analyses. in addition, t ... | 2016 | 26915496 |
| low temperature adaptation and the effects of cryoprotectants on mesorhizobia strains. | in this study, the tolerance of mesorhizobium sp. acmp18, mesorhizobium sp. usda3350, and mesorhizobium temperatum lmg23931 strains, to cold and freezing were investigated. the ability to withstand freezing at -20 °c and -70 °c for 24 months was different among the studied strains and depended on the cryoprotectant used. the survivability of mesorhizobial strains at -20 °c and -70 °c was significantly improved by some cryoprotectans (glycerol and sucrose/peptone). it is worth noting that the gre ... | 2016 | 26879468 |