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reclassification of bacteroides putredinis (weinberg et al., 1937) in a new genus alistipes gen. nov., as alistipes putredinis comb. nov., and description of alistipes finegoldii sp. nov., from human sources.during studies on the bacteriology of appendicitis in children, we often isolated from inflamed and non-inflamed tissue samples, an unusual bile-resistant pigment-producing strictly anaerobic gram-negative rod. phenotypically this organism resembles members of bacteroides fragilis group of species, as it is resistant to bile and exhibits a special-potency-disk pattern (resistance to vancomycin, kanamycin and colistin) typical for the b. fragilis group. however, the production of brown pigment on ...200312866844
alistipes onderdonkii sp. nov. and alistipes shahii sp. nov., of human origin.two groups of previously unknown gram-negative, strictly anaerobic, pigment-producing, rod-shaped bacteria, which phenotypically and phylogenetically displayed a close association with the recently described species alistipes finegoldii, were characterized using phenotypic and molecular taxonomic methods. a 16s rrna gene sequence divergence of approximately 3 % between the two unknown bacteria and a. finegoldii, as well as distinguishable biochemical characteristics, demonstrates that these orga ...200616902041
alistipes finegoldii in blood cultures from colon cancer patients. 200717953110
comparison of the cecal microbiota of domestic and wild turkeys.the extent to which production methods alter intestinal microbial communities of livestock is currently unknown. as the intestinal microbiota may affect animal health, nutrition, and food safety, a baseline comparison of the cecal communities of domestic and wild turkeys was performed. oligonucleotide fingerprinting of ribosomal rna (rrna) genes (ofrg) of 2,990 16s rrna clones and dot blot quantification of dominant populations were used to identify the dominant bacterial taxa. seventy-three per ...200818183454
application of several molecular techniques to study numerically predominant bifidobacterium spp. and bacteroidales order strains in the feces of healthy children.bifidobacteria and bacteroides-like bacteria are strictly anaerobic nonpathogenic members of human intestinal microflora. here we describe an analysis of the species and subspecies composition of these bacterial populations in healthy children using a combination of culture and molecular methods at two different time points. it was found that b. bifidum and b. longum are the most common dominant taxons in infants aged between 8 and 16 months. the majority of the infants carried several dominant ...200818323636
evaluation of the bacterial diversity in the feces of cattle using 16s rdna bacterial tag-encoded flx amplicon pyrosequencing (btefap).the microbiota of an animal's intestinal tract plays important roles in the animal's overall health, productivity and well-being. there is still a scarcity of information on the microbial diversity in the gut of livestock species such as cattle. the primary reason for this lack of data relates to the expense of methods needed to generate such data. here we have utilized a bacterial tag-encoded flx 16s rdna amplicon pyrosequencing (btefap) approach that is able to perform diversity analyses of ga ...200818652685
towards the human intestinal microbiota phylogenetic core.the paradox of a host specificity of the human faecal microbiota otherwise acknowledged as characterized by global functionalities conserved between humans led us to explore the existence of a phylogenetic core. we investigated the presence of a set of bacterial molecular species that would be altogether dominant and prevalent within the faecal microbiota of healthy humans. a total of 10 456 non-chimeric bacterial 16s rrna sequences were obtained after cloning of pcr-amplified rdna from 17 human ...200919601958
human gut bacterial communities are altered by addition of cruciferous vegetables to a controlled fruit- and vegetable-free diet.in the human gut, commensal bacteria metabolize food components that typically serve as energy sources. these components have the potential to influence gut bacterial community composition. cruciferous vegetables, such as broccoli and cabbage, contain distinctive compounds that can be utilized by gut bacteria. for example, glucosinolates can be hydrolyzed by certain bacteria, and dietary fibers can be fermented by a range of species. we hypothesized that cruciferous vegetable consumption would a ...200919640972
alistipes indistinctus sp. nov. and odoribacter laneus sp. nov., common members of the human intestinal microbiota isolated from faeces.two anaerobic, non-spore-forming, non-motile, gram-negative-staining bacteria, strains yit 12060(t) and yit 12061(t), were isolated from human faeces. cells of strain yit 12060(t) were coccoid to rod-shaped with round ends, positive for catalase, negative for indole and oxidase production, produced succinic and acetic acids as end products of glucose metabolism in peptone/yeast extract/glucose medium and had a dna g+c content of 55.2 mol%. the main respiratory quinones were mk-10 (40%) and mk-11 ...201019667375
re-assessment of phenotypic identifications of bacteroides putredinis to alistipes species using molecular methods.alistipes (previously bacteroides) are strictly anaerobic gram-negative rods that resemble the bacteroides fragilis group in that most species are bile-resistant and indole-positive; however, they are only weakly saccharolytic and most species produce light brown pigment only on laked rabbit blood agar. in this retrospective study, we re-identified 18 organisms previously identified phenotypically as "bacteroides putredinis-like", but that did not produce pigment on routine media. the strains we ...201121527349
identification and characterization of potential performance-related gut microbiotas in broiler chickens across various feeding trials.three broiler feeding trials were investigated in order to identify gut bacteria consistently linked with improvements in bird performance as measured by feed efficiency. trials were done in various geographic locations and varied in diet composition, broiler breed, and bird age. gut microbial communities were investigated using microbial profiling. eight common performance-linked operational taxonomic units (otus) were identified within both the ilea (180, 492, and 564-566) and ceca (140-142, 2 ...201121742925
gastrointestinal microbiome signatures of pediatric patients with irritable bowel syndrome.background & aims: the intestinal microbiomes of healthy children and pediatric patients with irritable bowel syndrome (ibs) are not well defined. studies in adults have indicated that the gastrointestinal microbiota could be involved in ibs. methods: we analyzed 71 samples from 22 children with ibs (pediatric rome iii criteria) and 22 healthy children, ages 7-12 years, by 16s ribosomal rna gene sequencing, with an average of 54,287 reads/stool sample (average 454 read length = 503 bases). data ...201121741921
species composition of bacteroidales order bacteria in the feces of healthy people of various ages.a study of species distribution of numerically predominant bacteroidales order isolates in feces of healthy people aged 1-33 years was accomplished using a combination of amplified ribosomal dna restriction analysis (ardra) and 16s ribosomal dna (rdna) sequencing. it was found that the majority of isolates in all age groups belonged to species b. xylanisolvens, b. vulgatus, and b. uniformis. members of genera alistipes, parabacteroides, odoribacter, barnesiella, and prevotella were also detected ...201222232251
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