| use of monoclonal antibodies in an elisa for detecting an invasive pest insect, liriomyza trifolii (diptera: agromyzidae). | a monoclonal antibody was prepared by the hybridoma technology. it reacted only with the protein of liriomyza trifolii (burgess) and not with that of chromatomyia horticola goureau or liriomyza sativae blanchard in indirect enzyme-linked immunosorbent assay. it was effective even after being diluted more than 8.192×10(6)-fold. the detection sensitivity of the antibody was 31.3 µg/ml under controlled conditions. positive reaction was achieved with all laboratory-reared l. trifolii samples, includ ... | 2015 | 26470159 |
| the leafminer liriomyza trifolii (diptera: agromyzidae) encapsulates its koinobiont parasitoid halticoptera circulus (hymenoptera: pteromalidae): implications for biological control. | the koinobiont parasitoid halticoptera circulus (walker) is a potential biological control agent of leafminers, but it has only rarely been collected from the invasive leafminer, liriomyza trifolii (burgess), in japan. to understand why this is the case, parasitism and development of h. circulus in l. trifolii was compared with parasitism and development in two indigenous leafminer species, liriomyza chinensis kato and chromatomyia horticola (goureau). there was no significant difference in para ... | 2016 | 26639841 |
| the mitochondrial genome of the garden pea leafminer chromatomyia horticola (goureau, 1851) (diptera: agromyzidae). | here we report the mitochondrial genome sequence of the garden pea leafminer chromatomyia horticola (goureau, 1851) (diptera: agromyzidae) (genbank accession no. kr047789). this is the first species with sequenced mitochondrial genome from the genus chromatomyia. the current length with partial a + t-rich region of this mitochondrial genome is 15,320 bp with an a + t content of 77.54%. all the 13 protein-coding, two rrna, and 22 trna genes were sequenced, except for the a + t-rich region. ... | 2016 | 26066024 |
| primer design for identifying economically important liriomyza species (diptera: agromyzidae) by multiplex pcr. | leafminer flies, especially, liriomyza huidobrensis, liriomyza sativae and liriomyza trifolii, are quarantine species in many countries. their morphological similarity makes identification difficult. to develop a rapid, reliable, sensitive and simple molecular identification method using multiplex pcr, we newly sequenced the mitochondrial cytochrome oxidase i (coi) genes of liriomyza bryoniae, liriomyza chinensis, l. huidobrensis, l. sativae, l. trifolii, chromatomyia horticola and four parasito ... | 2013 | 23039969 |
| polymerase chain reaction-restriction fragment length polymorphism assays to distinguish liriomyza huidobrensis (diptera: agromyzidae) from associated species on lettuce cropping systems in italy. | the pea leafminer, liriomyza huidobrensis (blanchard) (diptera: agromyzidae), is a serious insect pest infesting open field lettuce plantings in northern italy. in these cropping systems, it coexists with several other agromyzid species that have negligible economic importance on open field vegetables. the rapid detection of l. huidobrensis is crucial for effective management strategies, but the identification of agromyzids to species can be very difficult at adult as well at immature stages. in ... | 2006 | 16937681 |