Publications

TitleAbstractYear
Filter
PMID
Filter
light-dependent adsorption of photosynthetic cyanophages to synechococcus sp. wh7803.cyanophages infecting marine synechococcus strains are abundant in the world's oceans and are of considerable ecological significance by virtue of their hosts' role as prominent primary producers in the marine environment. in nature, cyanobacteria experience diel light-dark (ld) cycles, which may exert significant effects on the phage life cycle. an investigation into the role of light revealed that cyanophage s-pm2 adsorption to synechococcus sp. wh7803 was a light-dependent process. phage adso ...201020704597
oligonucleotide microarrays for bacteriophage expression studies.gene expression microarrays offer the ability to monitor the expression of all phage genes over an infection cycle. however, there are relatively few reports to date of microarrays being used to investigate phage biology. this chapter aims to provide an overview of how to design and implement a microarray experiment to investigate phage biology. given the nature of microarrays being specific to an organism, each will provide a number of unique issues. in this chapter, we outline the basic theory ...200919082558
the genome of s-pm2, a "photosynthetic" t4-type bacteriophage that infects marine synechococcus strains.bacteriophage s-pm2 infects several strains of the abundant and ecologically important marine cyanobacterium synechococcus. a large lytic phage with an isometric icosahedral head, s-pm2 has a contractile tail and by this criterion is classified as a myovirus (1). the linear, circularly permuted, 196,280-bp double-stranded dna genome of s-pm2 contains 37.8% g+c residues. it encodes 239 open reading frames (orfs) and 25 trnas. of these orfs, 19 appear to encode proteins associated with the cell en ...200515838046
cyanophage infection and photoinhibition in marine cyanobacteria.members of two cyanobacterial genera, synechococcus and prochlorococcus, are dominant within the prokaryotic component of the picophytoplankton and contribute significantly to global photosynthetic productivity. these organisms are known to be susceptible to infection by bacteriophages (viruses that infect bacteria) and it is believed that phage infection in the oceans has exerted selective pressures on the evolution of both phage and host and continues to influence community structure. understa ...200415501648
genetic organization of the psbad region in phages infecting marine synechococcus strains.the discovery of the genes psba and psbd, encoding the d1 and d2 core components of the photosynthetic reaction center psii (photosystem ii), in the genome of the bacteriophage s-pm2 (a cyanomyovirus) that infects marine cyanobacteria begs the question as to how these genes were acquired. in an attempt to answer this question, it was established that the occurrence of the genes is widespread among marine cyanomyovirus isolates and may even extend to podoviruses. the phage psba genes fall into a ...200415263091
a conserved genetic module that encodes the major virion components in both the coliphage t4 and the marine cyanophage s-pm2.sequence analysis of a 10-kb region of the genome of the marine cyanomyovirus s-pm2 reveals a homology to coliphage t4 that extends as a contiguous block from gene (g)18 to g23. the order of the s-pm2 genes in this region is similar to that of t4, but there are insertions and deletions of small orfs of unknown function. in t4, g18 codes for the tail sheath, g19, the tail tube, g20, the head portal protein, g21, the prohead core protein, g22, a scaffolding protein, and g23, the major capsid prote ...200111553768
an antisense rna in a lytic cyanophage links psba to a gene encoding a homing endonuclease.cyanophage genomes frequently possess the psba gene, encoding the d1 polypeptide of photosystem ii. this protein is believed to maintain host photosynthetic capacity during infection and enhance phage fitness under high-light conditions. although the first documented cyanophage-encoded psba gene contained a group i intron, this feature has not been widely reported since, despite a plethora of new sequences becoming available. in this study, we show that in cyanophage s-pm2, this intron is splice ...201020410936
infection by the 'photosynthetic' phage s-pm2 induces increased synthesis of phycoerythrin in synechococcus sp. wh7803.phycoerythrin-containing synechococcus strains are unicellular cyanobacteria that are of great ecological importance in the marine environment. these organisms are known to be susceptible to infection by cyanophages (viruses that infect cyanobacteria). the infection cycle takes several hours and during this time the cyanophages may potentially modify the cyanobacterial light-harvesting apparatus. this study based on a model system consisting of synechococcus sp. wh7803 and cyanophage s-pm2 revea ...200818479290
Displaying items 1 - 8 of 8