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diaphorobacter nitroreducens gen nov, sp nov, a poly(3-hydroxybutyrate)-degrading denitrifying bacterium isolated from activated sludge.three denitrifying strains of bacteria capable of degrading poly(3-hydroxybutyrate) (phb) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (phbv) were isolated from activated sludge and characterized. all of the isolates had almost identical phenotypic characteristics. they were motile gram-negative rods with single polar flagella and grew well with simple organic compounds, as well as with phb and phbv, as carbon and energy sources under both aerobic and anaerobic denitrifying conditions. howev ...200212682868
[isolation and characterization of a p-chloroaniline-degrading bacterial strain].a bacterial strain that degrades p-chloroaniline is isolated from activated sludge of wastewater treatment plant treating wastewater from a chemical factory. results indicate that this strain took p-chloroaniline as sole carbon, nitrogen and energy sources. it is identified as diaphorobacter sp. pca039 according to its morphology, and biochemical properties, and 16s rdna sequence analysis. further study indicate that chloride anion is released simultaneously and stoichemically to p-chloroaniline ...200515859428
[a novel metabolism pathway for the biodegradation of chloroanilines].up to date, p-chloroaniline was reported to be biodegraded only through ortho-or modified ortho-cleavage pathway. in this paper, the intermediates of p-chloroaniline metabolized by strain diaphorobacter pca039 were analyzed and identified by hplc and lc/ms methods. the results showed that intermediates such as 4-chlorocatechol, 2-hydroxy-5-chloromuconic semialdehyde, 5-chloro-4-oxalocrotonate, 5-chloro-2-oxo-4-hydroxypentanote, chloro-acetate were formed and consumed in order during the degradat ...200717436630
simultaneous nitrification and denitrification by diverse diaphorobacter sp.eight bacterial isolates closely related to diaphorobacter sp. were isolated from activated biomass surviving on wastewater laden with dyes and nitro-substituted chemicals and were identified by 16s rdna sequence analysis. the isolates showed sequence similarity of 99-100% to other diaphorobacter strains such as zy 2006b, f2, na5, pca039, d. nitroreducens ksp4, and ksp3 and 98-99% sequence homology to d. nitroreducens na10b (type strain jcm 11421). neighbor-joining tree revealed that all the eig ...200717876578
pseudacidovorax intermedius gen. nov., sp. nov., a novel nitrogen-fixing betaproteobacterium isolated from soil.a gram-negative, short rod-shaped, nitrogen-fixing bacterium (cc-21(t)) was isolated from a soil sample collected from the regional agricultural research station in kaohsiung county (taiwan). using 16s rrna gene sequence analysis, it could be clearly demonstrated that this isolate was novel: it showed <97 % similarity to species of the genera acidovorax, alicycliphilus, giesbergeria, simplicispira and diaphorobacter. the organism used several organic acids, but only a few sugars as substrates. t ...200818218955
molecular characterization and in situ quantification of anoxic arsenite-oxidizing denitrifying enrichment cultures.to explore the bacteria involved in the oxidation of arsenite (as(iii)) under denitrifying conditions, three enrichment cultures (ecs) and one mixed culture (mc) were characterized that originated from anaerobic environmental samples. the oxidation of as(iii) (0.5 mm) was dependent on no(3) (-) addition and n(2) formation was dependent on as(iii) addition. the ratio of n(2)-n formed to as(iii) fed approximated the expected stoichiometry of 2.5. a 16s rrna gene clone library analysis revealed thr ...200919187211
diaphorobacter oryzae sp. nov., isolated from a thiosulfate-oxidizing enrichment culture.a taxonomic study was performed on two isolates, strains rf3(t) and rf21, recovered from a thiosulfate-oxidizing enrichment culture. comparative 16s rrna gene sequence analysis showed that these isolates were clearly affiliated with the class betaproteobacteria. strains rf3(t) and rf21 were most closely related to diaphorobacter nitroreducens na10b(t) (97.9 and 98.2 % 16s rrna gene sequence similarity, respectively). the level of 16s rrna gene sequence similarity between strains rf3(t) and rf21 ...200919196757
molecular characterization of early colonizer bacteria from wastes in a steel plant.forty-nine bacteria isolated from four newly-produced waste samples of a steel industry, which had a high content of cao, mgo, cr and p2o5, were characterized molecularly and phenotypically by susceptibility testing against heavy metals.200819241517
broad-range pcr, cloning and sequencing of the full 16s rrna gene for detection of bacterial dna in synovial fluid samples of tunisian patients with reactive and undifferentiated arthritis.broad-range rdna pcr provides an alternative, cultivation-independent approach for identifying bacterial dna in reactive and other form of arthritis. the aim of this study was to use broad-range rdna pcr targeting the 16s rrna gene in patients with reactive and other forms of arthritis and to screen for the presence of dna from any given bacterial species in synovial fluid (sf) samples.200919570210
bacterial community structure and dominant bacteria in activated sludge from a 70 degrees c ultrasound-enhanced anaerobic reactor for treating carbazole-containing wastewater.status of, and changes in, the bacterial communities at two acclimation stages (with- and without-ultrasound) in a small 70 degrees c ultrasound-enhanced anaerobic reactor for treating carbazole-containing wastewater reactor were analyzed by pcr-dgge and real-time pcr techniques. pcr-dgge results indicated that a large number of bands occurred in the whole sludge samples. pseudomonas sp., comamonas sp., and diaphorobacter sp. were identified as being able to utilize carbazole as a carbon source, ...201019733065
two novel pyrene-degrading diaphorobacter sp. and pseudoxanthomonas sp. isolated from soil.two new bacterial strains, kotlb and rn402, which have the ability to utilize pyrene as their sole source of carbon and energy, were isolated from soil. strains kotlb and rn402 degraded 99% of 100 mg/l of pyrene in liquid cultures within 16 days. besides pyrene, both strains also degraded 100 mg/l of phenanthrene almost completely within 8 days and degraded 99% and 55% of 100 mg/l of fluoranthene within 24 days, respectively. 16s rdna-based phylogenetic analysis suggested that strains kotlb and ...200919914581
novel tn4371-ice like element in ralstonia pickettii and genome mining for comparative elements.integrative conjugative elements (ices) are important factors in the plasticity of microbial genomes. an element related to the ice tn4371 was discovered during a bioinformatic search of the ralstonia pickettii 12j genome. this element was analysed and further searches carried out for additional elements.a pcr method was designed to detect and characterise new elements of this type based on this scaffold and a culture collection of fifty-eight ralstonia pickettii and ralstonia insidiosa strains ...200919941653
molecular characterization of the polymicrobial flora in chronic rhinosinusitis.conventional cultures have implicated staphylococcus aureus (sa) and coagulase-negative staphylococcus (cns) as principal pathogens in chronic rhinosinusitis (crs). these results are questioned by recent studies in which molecular probes implicate haemophilus influenzae instead.201020211106
diagnosis and quantification of glycerol assimilating denitrifying bacteria in an integrated fixed-film activated sludge reactor via 13c dna stable-isotope probing.glycerol, a byproduct of biodiesel and oleo-chemical manufacturing operations, represents an attractive alternate to methanol as a carbon and electron donor for enhanced denitrification. however, unlike methanol, little is known about the diversity and activity of glycerol assimilating bacteria in activated sludge. in this study, the microbial ecology of glycerol assimilating denitrifying bacteria in a sequencing batch integrated fixed film activated sludge (sb-ifas) reactor was investigated usi ...201021053955
identification of the biochemical degradation pathway of triazophos and its intermediate in diaphorobacter sp. tpd-1.triazophos is one of the most widely used organophosphorus insecticides usually detectable in the environment. a bacterial strain, diaphorobacter sp. tpd-1, capable of using triazophos and its intermediate, 1-phenyl-3-hydroxy-1,2,4-triazole (pht), as its sole carbon sources for growth was isolated from a triazophos-contaminated soil in china. this strain could completely degrade 50 mg l(-1) triazophos and pht to non-detectable level in 24 and 56 h, respectively. during pht degradation, three met ...201121212954
adsorption and degradation of triazophos, chlorpyrifos and their main hydrolytic metabolites in paddy soil from chaohu lake, china.triazophos and chlorpyrifos are organophosphorus pesticides (ops), and their primary hydrolytic metabolites are 1-phenyl-3-hydroxy-1,2,4-triazole (bzc) and 3,5,6-trichloro-2-pyridinol (tcp). in this study, the adsorption and degradation of triazophos, chlorpyrifos, bzc and tcp were investigated in paddy soil from chaohu lake, china. adsorption tests demonstrated that the adsorption of these compounds to soils could be described by the freundlich equation. moreover, chlorpyrifos displayed the hig ...201121592646
cultivation-independent analysis of archaeal and bacterial communities of the formation water in an indian coal bed to enhance biotransformation of coal into methane.biogenic origin of the significant proportion of coal bed methane has indicated the role of microbial communities in methanogenesis. by using cultivation-independent approach, we have analysed the archaeal and bacterial community present in the formation water of an indian coal bed at 600-700 m depth to understand their role in methanogenesis. presence of methanogens in the formation water was inferred by epifluorescence microscopy and pcr amplification of mcra gene. archaeal 16s rrna gene clone ...201122202965
flexible bacterial strains that oxidize arsenite in anoxic or aerobic conditions and utilize hydrogen or acetate as alternative electron donors.arsenic is a carcinogenic compound widely distributed in the groundwater around the world. the fate of arsenic in groundwater depends on the activity of microorganisms either by oxidizing arsenite (as(iii)), or by reducing arsenate (as(v)). because of the higher toxicity and mobility of as(iii) compared to as(v), microbial-catalyzed oxidation of as(iii) to as(v) can lower the environmental impact of arsenic. although aerobic as(iii)-oxidizing bacteria are well known, anoxic oxidation of as(iii) ...201121706372
comparison of denitrification between paracoccus sp. and diaphorobacter sp.denitrification was compared between paracoccus sp. and diaphorobacter sp. in this study, both of which were isolated from activated sludge of a denitrifying reactor. denitrification of both isolates showed contrasting patterns, where diaphorobacter sp. showed accumulation of nitrite in the medium while paracoccus sp. showed no accumulation. the nitrate reduction rate was 1.5 times more than the nitrite reduction in diaphorobacter sp., as analyzed by the resting state denitrification kinetics. i ...201121509603
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