| [study on the test and evaluation of the toxicity of the strains preparation of the different subspecies of bacillus thuringiensis and on the research into its standardization]. | in this paper using the preparation of bacillus thuringiensis of usa standard sample-subsp. kurstaki hd-1-s-1980 (h3a3b) the toxicity to different subspecies preparations such as subsp. dendrolimus u strain(h4a4b), subsp. galleriae c88 strain (h5a5b) is tested and evaluated. and product's standardization is also researched. comparing their toxicity to various test-insects between the standard sample prepared from u strain and usa, standard sample, the boilogical determined results are as follows ... | 1996 | 9206272 |
| caterpillar setae in the deep cornea and anterior chamber. | to report a case of caterpillar setae embedded in the deep cornea and anterior chamber. | 2000 | 10704559 |
| transgenic loblolly pine (pinus taeda l.) plants expressing a modified delta-endotoxin gene of bacillus thuringiensis with enhanced resistance to dendrolimus punctatus walker and crypyothelea formosicola staud. | a synthetic version of the cry1ac gene of bacillus thuringiensis has been used for the transformation of loblolly pine (pinus taeda l.) using particle bombardment. mature zygotic embryos were used to be bombarded and to generate organogenic callus and transgenic regenerated plants. expression vector pb48.215 dna contained a synthetic bacillus thuringiensis (b.t.) cry1ac coding sequence flanked by the double cauliflower mosaic virus (camv) 35s promoter and nopaline synthase (nos) terminator seque ... | 2003 | 12554726 |
| genomic sequence analyses of segments 1 to 6 of dendrolimus punctatus cytoplasmic polyhedrosis virus. | the complete nucleotide sequences of genomic segments s1 to s6 from dendrolimus punctatus cypovirus 1 (dpcpv-1) have been determined. each segment of s1 to s6 possess a single open reading frame. conserved motifs 5' (aguaa) and 3'(guuagcc) were found at the ends of each segment. comparison of the proteins of dpcpv with those of other members in the family reoviridae lead us to suggest that s1, s3, s4 and s6 encode the viral structural protein vp1, vp2, vp3 and vp4, respectively. s5 encoded viral ... | 2003 | 12827465 |
| nucleotide sequences of genome segments s6, s7 and s10 of dendrolimus punctatus cypovirus 1. | the nucleotide sequences of genome segments s6, s7 and s10 of dendrolimus punctatus cypovirus 1 hunan i (dpcpv-hn(i)) and dpcpv-hn(i)-se(3) (dpcpv-hn(i) passed three times in spodoptera exigua) were determined. segment s10 was 944 nucleotides in length and encoded a polyhedrin of 248 amino acids (28,439 da). only two nucleotide mutations were found between dpcpv-hn(i) s10 and dpcpv-hn(i)-se3 s10, and the deduced amino acid sequences of the polyhedrin proteins were identical. segment s7, 1 501 nu ... | 2004 | 14689283 |
| molecular monitoring and evaluation of the application of the insect-pathogenic fungus beauveria bassiana in southeast china. | to monitor the fate of inundatively applied strains of beauveria bassiana against masson's pine caterpillar, dendrolimus punctatus, in magushan forest, southeast china, and evaluate the potential environmental risks of biocontrol introduction. | 2004 | 15012826 |
| mapping the rna-binding domain on the dpcpv vp4. | the rna-binding properties of vp4 protein of dendrolimus punctatus cytoplasmic polyhedrosis virus (dpcpv) vp4 were analyzed. vp4 was expressed in e. coli and assayed for rna binding activity by gel mobility shift assay. vp4 was found to bind rna (ssrna and dsrna) in a sequence-independent manner, but did not interact with dna. to identify the domain(s) of the protein important for rna binding, a number of deletions were made and tested by gel mobility shift assays and northwestern blot. the cent ... | 2006 | 16195791 |
| insecticidal evaluation of beauveria bassiana engineered to express a scorpion neurotoxin and a cuticle degrading protease. | to improve the insecticidal efficacy of the entomopathogen beauveria bassiana, the fungus was genetically modified with an insect-specific scorpion neurotoxin aait and an insect cuticle degrading protease pr1a from another insect pathogen (metarhizium anisopliae). the wild-type and the transformants were bioassayed against the larvae of masson's pine caterpillar dendrolimus punctatus and the wax moth galleria mellonella. in comparison to the wild-type strain, engineered isolates took fewer spore ... | 2008 | 18800183 |
| genomic and biological characterization of a new cypovirus isolated from dendrolimus punctatus. | a novel cypovirus (designated dpcpv-mc) was isolated from the pine moth dendrolimus punctatus using serial in vivo cloning procedures. dpcpv-mc occurs in typical polyhedral occlusion bodies, containing a number of spherical virions. laboratory bioassays indicated that the infectivity of dpcpv-mc against second-instar spodoptera exigua larvae does not differ significantly from that of dendrolimus punctatus cypovirus 1. full-length amplification of the dpcpv-mc cdnas identified 16 dsrna genome seg ... | 2014 | 25419713 |
| interactions among dendrolimus punctatus cypovirus proteins and identification of the genomic segment encoding its a-spike. | revealing the interactions among cypovirus proteins would facilitate our understanding of the replication and assembly of this virus. in the present study, interactions among proteins encoded by the 10 segments of dendrolimus punctatus cypovirus (dpcpv) were identified using yeast two-hybrid (y2h) and far-western blotting assays. in total, 24 pairs of interactions were detected. twelve pairs of one-direction interactions, four pairs of binary interactions and four pairs of self-associations were ... | 2014 | 24700101 |
| effect of needle damage on the release rate of masson pine (pinus massoniana) volatiles. | the effect of needle damage on the release rate of masson pine (pinus massoniana lamb.) volatiles was examined. needles were continuously damaged by mechanical damage (mdp) or by feeding of pine caterpillar (dendrolimus punctatus) larvae (lfp); undamaged pine was used as a control (udp). volatiles were collected before damage, and at 16, 24, 40, 48, 64, 72, 88 and 96 h post-damage, and analyzed. the analyses revealed that 19 compounds identified as constitutive volatiles from udp were terpenes a ... | 2009 | 19067110 |
| rna-binding properties of dendrolimus punctatus tetravirus p17 protein. | the rna-binding properties of the p17 protein of dendrolimus punctatus tetravirus were analysed. we have demonstrated that p17 protein, a nonstructural protein with a potentially important role in viral replication, is a rna-binding protein, which has not been previously described for any member of the family tetraviridae. p17 protein was expressed in escherichia coli and was used in uv cross-linking analysis, using a digoxigenin-utp-labeled rna probe and chemical cross-linking analysis. the ana ... | 2008 | 18945507 |
| development and characterization of a continuous cell line, afkm-on-h, from hemocytes of the european corn borer ostrinia nubilalis (hübner) (lepidoptera, pyralidae). | the corn borer, ostrinia nubilalis, is a very important pest in different countries, and the in vitro system of the insect could be a useful tool for isolation and characterization of the pathogens and physiological responses of the insect. in this context, a cell line was derived from the hemocytes of the european corn borer and was named afkm-on-h for, respectively, o. nubilalis, armand frappier, king mongkut institutes, and hemocytes. this cell line was initiated and maintained in ex-cell 400 ... | 2007 | 17846857 |
| characterisation and partial sequence analysis of two novel cypoviruses isolated from the winter moth operophtera brumata (lepidoptera: geometridae). | the complete nucleotide sequences of genomic segments s5 to s10 from operophtera brumata cypovirus 18 (opbucpv18), and the complete nucleotide sequences of genomic segments s2, s5, s9 and s10 from operophtera brumata cypovirus 19 (opbucpv19) have been determined. each genome segment contained a single open reading frame (orf). conserved motifs 5' (aguaaa....guuagcu) 3' were found at the ends of each segment of opbucpv18, whilst conserved motifs 5' (aacaaa....uuugc) 3' were found at each segment ... | 2007 | 17546493 |
| expression and characterization of rna-dependent rna polymerase of dendrolimus punctatus tetravirus. | dendrolimus punctatus tetravirus (dptv) has been identified as a new member of the genus omegatetravirus of the family tetraviridae that may be related serologically to nudaurelia capensis virus (nomegav). to establish the function of dptv rna genome and to better understand the mechanism of viral replication, the putative rna-dependent rna polymerase (rdrp) domain has been cloned and expressed in escherichia coli. the recombinant protein was purified on a ni-chelating histrap affinity column an ... | 2006 | 17002878 |
| stimulation of sex pheromone production by pban-like substance in the pine caterpillar moth, dendrolimus punctatus (lepidoptera: lasiocampidae). | sex pheromone production in the female pine caterpillar moth, dendrolimus punctatus is controlled by a pban-like substance located in the head of female moth. pheromone titer was significantly decreased by decapitation of female moth, and restored by injection of either hez-pban or head extract prepared from male or female moth. stimulation of pheromone production by head extract followed a dose-dependent pattern from 0.5 to at least 4 head equivalent. a gland in vitro assay was used to study th ... | 2002 | 11857674 |
| identification of transmembrane domain of a membrane associated protein ns5 of dendrolimus punctatus cytoplasmic polyhedrosis virus. | we examined the intracellular localization of ns5 protein of dendrolimus punctatus cytoplasmic polyhedrosis virus (dpcpv) by expressing ns5-gfp fusion protein and proteins from deletion mutants of ns5 in baculovirus recombinant infected insect spodoptera frugiperda (sf-9) cells. it was found that the ns5 protein was present at the plasma membrane of the cells, and that the n-terminal portion of the protein played a key role in the localization. a transmembrane region was identified to be present ... | 2006 | 16889685 |
| [molecular cloning and sequencing of dendrolimus punctatus wenshanensis cytoplasmic polyhedrosis virus (dpwcpv) ns5 protein gene]. | dpwcpv were purified from infected dendrolimus punctatus wenshanensis larvae by density gradient centrifugation. the genomic dsrna segments were subsequently extracted directly from the purified polyhedra by sds treatmen, phenol/chloroform extraction and ethanol precipitation. defferent segments of genomic dsrna were separated by low melting point agarose electrophoresis, and s9 dsrna was purified after being excised from the gel. s9 rna was denaturalized in high temperature. the primers was des ... | 2003 | 16276867 |
| characterization of the rna-binding domain in the dendrolimus punctatus cytoplasmic polyhedrosis virus nonstructural protein p44. | dendrolimus punctatus cytoplasmic polyhedrosis virus (dpcpv-1) belongs to the cypovirus genus in the reoviridae family. the orf of genome segment 8 (s8) of dpcpv-1 was cloned into vector pmal-c2x and used to express a 44kda protein (p44) in e. coli, which was detected by western blotting. the gel mobility shift assays showed that p44 had ssrna-binding activity. competitive assay indicated that this protein only bind to ssrna and could not interact with dna and dsrna. the binding of p44 to ssrna ... | 2005 | 16023751 |
| [effects of ultra low volume beauveria bassiana oil formulation on biological diversity]. | in 2001-2003, an ultra low volume (ulv) beauveria bassiana (bb) oil formulation was used to prevent against dendrolimus punctatus, and its effect on biological diversity (species richness, individual number, species diversity index and evenness index, and dominant index) was investigated. the results showed that there were 473, 392 and 266 species in non-control, bb control and chemical control areas, respectively. the individual number ratio of natural enemies to pest in bb control area (0.1049 ... | 2004 | 15825449 |
| isolation and identification of a new tetravirus from dendrolimus punctatus larvae collected from yunnan province, china. | in this study, dendrolimus punctatus tetravirus (dptv) has been identified as a new member of the genus omegatetravirus of the family tetraviridae that may be related serologically to nudaurelia capensis omega virus (nomegav). dptv particles are isometric, with a diameter of about 40 nm and a buoyant density of 1.281 g cm(-3) in cscl. the virus has two capsid proteins (of 62 500 and 6800 da) and two single-stranded rna molecules (rna1 and rna2), which are 5492 and 2490 nt long, respectively. rna ... | 2005 | 15722541 |
| molecular characterization of segments 7-10 of dendrolimus punctatus cytoplasmic polyhedrosis virus provides the complete genome. | the nucleotide sequences of genomic segments s7-s10 from dendrolimus punctatus cypovirus strain hunan (dpcpv-hn) have been determined. this provides the complete genome sequences of dpcpv-hn. each segment of s7-s10 possess a single segment each. homology searches showed that the nucleotide sequences and the deduced amino acid sequences of dpcpv s7-10 had high level of identities with those of bombyx mori cypovirus (bmcpv) s7-10, respectively. while the amino acid sequences of the proteins encode ... | 2003 | 12837553 |
| [correspondence analysis between occurrence type of dendrolimus punctatus and topographical and vegetative features]. | the relationship between the occurrence type of dendrolimus punctatus and the geographical and vegetative features in subcompartments was analyzed using correspondence analysis and shown by a figure of cluster. the results showed that the majority subcompartments in which d. punctatus occurred frequently were at low elevation and low slope, and distributed among low hills or middle hills, while the subcompartments at high elevation or high slopes, or in low mountains or middle mountains were saf ... | 2002 | 12561192 |
| cysteine protease enhances plant-mediated bollworm rna interference. | oral ingestion of plant-expressed double stranded rna (dsrna) triggers target gene suppression in insect. an important step of this process is the transmission of dsrna from plant to midgut cells. insect peritrophic matrix (pm) presents a barrier that prevents large molecules from entering midgut cells. here, we show that uptake of plant cysteine proteases, such as ghcp1 from cotton (gossypium hirsutum) and atcp2 from arabidopsis, by cotton bollworm (helicoverpa armigera) larvae resulted in atte ... | 2013 | 23460027 |
| [spectral features analysis of pinus massoniana with pest of dendrolimus punctatus walker and levels detection]. | taking 51 field measured hyperspectral data with different pest levels in yanping, fujian province as objects, the spectral reflectance and first derivative features of 4 levels of healthy, mild, moderate and severe insect pest were analyzed. on the basis of 7 detecting parameters construction, the pest level detecting models were built. the results showed that (1) the spectral reflectance of pinus massoniana with pests were significantly lower than that of healthy state, and the higher the pest ... | 2013 | 23697126 |
| mitochondrial phylogenomics and genetic relationships of closely related pine moth (lasiocampidae: dendrolimus) species in china, using whole mitochondrial genomes. | pine moths (lepidoptera; bombycoidea; lasiocampidae: dendrolimus spp.) are among the most serious insect pests of forests, especially in southern china. although coi barcodes (a standardized portion of the mitochondrial cytochrome c oxidase subunit i gene) can distinguish some members of this genus, the evolutionary relationships of the three morphospecies dendrolimus punctatus, d. tabulaeformis and d. spectabilis have remained largely unresolved. we sequenced whole mitochondrial genomes of eigh ... | 2015 | 26040695 |
| transcriptome characterization of dendrolimus punctatus and expression profiles at different developmental stages. | the pine moth dendrolimus punctatus (walker) is a common insect pest that confers serious damage to conifer forests in south of china. extensive physiology and ecology studies on d. punctatus have been carried out, but the lack of genetic information has limited our understanding of the molecular mechanisms behind its development and resistance. using rna-seq approach, we characterized the transcriptome of this pine moth and investigated its developmental expression profiles during egg, larval, ... | 2016 | 27560151 |
| cloning of three genes involved in the flavonoid metabolic pathway and their expression during insect resistance in pinus massoniana lamb. | pinus massoniana lamb. is an important timber and turpentine-producing tree species in china. dendrolimus punctatus and dasychira axutha are leaf-eating pests that have harmful effects on p. massoniana production. few studies have focused on the molecular mechanisms underlying pest resistance in p. massoniana. based on sequencing analysis of the transcriptomes of insect-resistant p. massoniana, three key genes involved in the flavonoid metabolic pathway were identified in the present study (pmf3 ... | 2016 | 28081282 |
| genome editing of wnt-1, a gene associated with segmentation, via crispr/cas9 in the pine caterpillar moth, dendrolimus punctatus. | the pine caterpillar moth, dendrolimus punctatus, is a devastating forest pest. genetic manipulation of this insect pest is limited due to the lack of genomic and functional genomic toolsets. recently, crispr/cas9 technology has been demonstrated to be a promising approach to modify the genome. to investigate gene functions during the embryogenesis, we introduced crispr/cas9 system in d. punctatus to precisely and effectively manipulate gene expressions inmutant embryos. compared to controls, kn ... | 2016 | 28111552 |
| identification, expression pattern, and feature analysis of cuticular protein genes in the pine moth dendrolimus punctatus (lepidoptera: lasiocampidae). | cuticular proteins (cps) are vital components of the insects' cuticle that support movement and protect insect from adverse environmental conditions. the cps exist in a large number and diversiform structures, thus, the accurate annotation is the first step to interpreting their roles in insect growth. the rapid development of sequencing technology has simplified the access to the information on protein sequences, especially for non-model species. dendrolimus punctatus is a lepidopteran defoliat ... | 2017 | 28284855 |
| mtase domain of dendrolimus punctatus cypovirus vp3 mediates virion attachment and interacts with host alp protein. | dendrolimus punctatus cypovirus (dpcpv) is an important pathogen of d. punctatus, but little is known about the mechanisms of dpcpv infection. here, we investigated the effects of vp3, vp4 and vp5 structural proteins on the viral invasion. both the c-terminal of vp3 (methyltransferase (mtase) domain) and vp4 (a-spike) bound to spodoptera exigua midgut brush border membrane vesicles (bbmvs) in a dose-dependent manner, and the binding was inhibited by purified dpcpv virions. importantly, anti-mtas ... | 2017 | 28368302 |
| nucleotide sequence and genomic organization of a newly isolated densovirus infecting dendrolimus punctatus. | the nucleotide sequence of a novel icosahedral dna virus infecting dendrolimus punctatus has been determined. the genome is 5039 nt long and includes inverted terminal repeats of 200 nt containing 131 nt long j-shaped terminal hairpins. the 'plus' strand of the genome contains three large open reading frames (orfs), the left and the mid-orfs (within the left orf) in the left-half encoding the non-structural proteins and the right orf in the right-half encoding viral capsid proteins. ns1 protein ... | 2005 | 16033964 |
| molecular characterization of genome segments 1 and 3 encoding two capsid proteins of antheraea mylitta cytoplasmic polyhedrosis virus. | antheraea mylitta cytoplasmic polyhedrosis virus (amcpv), a cypovirus of reoviridae family, infects indian non-mulberry silkworm, antheraea mylitta, and contains 11 segmented double stranded rna (s1-s11) in its genome. some of its genome segments (s2 and s6-s11) have been previously characterized but genome segments encoding viral capsid have not been characterized. | 2010 | 20684765 |
| elucidation of the sex-pheromone biosynthesis producing 5,7-dodecadienes in dendrolimus punctatus (lepidoptera: lasiocampidae) reveals delta 11- and delta 9-desaturases with unusual catalytic properties. | sex pheromones produced by female moths of the lasiocampidae family include conjugated 5,7-dodecadiene components with various oxygenated terminal groups. here we describe the molecular cloning, heterologous expression and functional characterization of desaturases associated with the biosynthesis of these unusual chemicals. by homology-based pcr screening we characterized five cdnas from the female moth pheromone gland that were related to other moth desaturases, and investigated their role in ... | 2010 | 20403437 |
| [effects of dendrolimus punctatus feeding and methyl jasmonate (meja)--or terpenes fumigation on abscisic acid and jasmonic acid contents in pinus massoniana seedling needles]. | abscisic acid (aba) and jasmonic acid (ja) play an important role in inducing the stress-resistance of plants. in this study, parts of the needles on a ring of pinus massoniana seedling shoots were subjected to 4 hours dendrolimus punctatus feeding or 4 hours fumigation with 10 micromol x l(-1) of methyl jasmonate (meja) or terpenes, and the aba and ja contents in treated needles, untreated neighboring sister needles, and untreated needles above and below the ring were determined by gc/ms. an ob ... | 2009 | 19803176 |
| identification and characterization of rna duplex unwinding and atpase activities of an alphatetravirus superfamily 1 helicase. | dendrolimus punctatus tetravirus (dptv) belongs to the genus omegatetravirus of the alphatetraviridae family. sequence analysis predicts that dptv replicase contains a putative helicase domain (hel). however, the helicase activity in alphatetraviruses has never been formally determined. in this study, we determined that dptv hel is a functional rna helicase belonging to superfamily-1 helicase with 5'-3' dsrna unwinding directionality. further characterization determined the length requirement of ... | 2012 | 22995190 |
| molecular characterization of protein p50 of dendrolimus punctatus cytoplasmic polyhedrosis virus. | genome segment 7 of the 10-segmented rna genomes of dendrolimus punctatus cytoplasmic polyhedrosis virus (dpcpv) comprises 1502 nucleotides with one orf of 1347 bp. this orf was predicted to encode a protein of 448 amino acids with a molecular mass of 49,756 da (p50). antisera against both p50 and an antigen domain (ad) near the n-terminus of p50 specifically bound to a viral structural protein of ca. 33 kda (v5), indicating that v5 was an n-terminal product of p50. immunoblotting analysis with ... | 2013 | 22581499 |
| [effects of environmental temperature on dendrolimus punctatus development and survival]. | in a simulation test, three treatments of low, normal and high temperature were designed to examine the effects of environmental fluctuant temperature on the development and survival of second generation dendrolimus punctatus larvae. the results showed that under different temperature but same light and dark cycle, the mortality of lst to approximately 3rd instar was apparently different. under long photophase, the larval mortality of 1st to approximately 3rd instar was 25.55%, 55.13% and 58.23% ... | 2006 | 16724747 |
| sex pheromone biosynthesis in the pine caterpillar moth, dendrolimus punctatus (lepidoptera: lasiocampidae): pathways leading to z5-monoene and 5,7-conjugated diene components. | biosynthesis of the sex pheromone components (z)-5-dodecenol and (z,e)-5,7-dodecadienol in dendrolimus punctatus was studied by topical application of deuterium-labeled fatty acids to pheromone glands and subsequent analysis of fatty acyl groups and pheromone components by gas chromatography-mass spectrometry. our studies suggest that both (z)-5-dodecenol and (z,e)-5,7-dodecadienol can be biosynthetically derived from chain elongation of palmitate to stearate in the gland, and its subsequent del ... | 2004 | 14871622 |
| pinemoth caterpillar disease. | pinemoth caterpillar disease is a well-recognised disorder which occurs in south-eastern china and is due to contact with the cocoons or larvae of the pinemoth (dendrolimus punctatus). generalised symptoms, which may be noted within two weeks of contact, consist of pyrexia, anorexia, malaise, rigors, headache, or dizziness. localised abnormalities include dermatologic changes, skin nodules, and arthritis. the arthritis is usually monoarticular and most frequently involves the knee, ankle, and wr ... | 1986 | 3094159 |
| [cloning and mapping of dpkettin gene on z chromosome of the pine caterpillar by real-time quantitative pcr]. | the ratio of z chromosome number to autosomal chromosome (a) is different between male and female lepidoptera insects. in males: 2z : 2a=1, and in females: z : 2a=0.5. the ratio of the copies of gene (such as k) on z chromosome number to the copies of gene (such as n) on autosomal chromosome (k/n) is also different between male and female. in males: 2k : 2n =1, and in females: k : 2n=0.5. the dpkettin gene of the pine caterpillar dendrolimus punctatus was cloned with the primers designed accordi ... | 2007 | 17646148 |