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a cross-sectional survey of gastrointestinal parasites with dispersal stages in feces from costa rican dairy calves.a cross-sectional study was carried out to determine the prevalence of gastrointestinal parasites and lungworm nematodes in dairy calves from five different ecoclimatic areas of costa rica. also intensity of infection of nematodes was determined. in order to describe management practices and anthelmintic control, a questionnaire was applied in 73 farms. the influence of area, farm, host (breed, age) and ecological factors (low and high rainfall period) upon eggs per gram feces (epg) of gastroint ...201020810217
synlophe patterns of the haemonchinae of ruminants (nematoda: trichostrongyloidea).the pattern of longitudinal ridges (synlophe) on the external cuticular surface of trichostrongylid nematodes has been shown to be of value for distinguishing species and determining relationships among higher taxa. in the process of studying mecistocirrus digitatus, the large stomach worm of bovids of asia that has been imported and established in the americas, we observed remarkably similar synlophe patterns to those described for 3 species of haemonchus and to those we examined in a species o ...200011128486
examination of gastrointestinal helminth in livestock grazing in grassland of bangladesh.to determine association of grassland with parasitic diseases of livestock in bangladesh, the 'tracer' animals (two cow calves and two goats) were released for a month in a grassland used for communal grazing of livestock near school premise in kanthal, trishal, mymensingh, bangladesh. after slaughtering of the tracer animals, their gastrointestinal tract examination revealed six species of nematode and one cestode. the nematode species were haemonchus contortus, trichostrongylus axei, mecistoci ...200011002657
pathophysiological aspects of mecistocirrus digitatus (nematoda: trichostrongylidae) infection in calves.three groups of four calves were experimentally infected with infectious larvae of mecistocirrus digitatus. one group received a trickle infection of 5000 l3 per day for 8 days, the other two groups received a single infection of 5000 and 40,000 l3, respectively. all animals were necropsied 120 days after infection. prepatent periods varied between 61 and 79 days, and maximal faecal egg output was reached between 80 and 100 days after infection. repeated infections were not additive and worm cou ...19979195735
bilateral, perivulval cuticular pores in trichostrongylid nematodes.a new hypodermal gland was discovered in female nematodes of the family trichostrongylidae. because the new structure appears to be associated with the vulva, it was named the perivulval pore. it is similar, based on light and scanning electron microscopy, to phasmids that are located laterally on the tails of nematodes of the class secernentea. like phasmids, perivulval pores are paired and bilateral, with cuticular ducts to the surface in the areas of the lateral chords. they are located sligh ...19957623208
pcr-based species-specific amplification of its of mecistocirrus digitatus and its application in identification of gi nematode eggs in bovine faeces.differential diagnosis of mecistocirrus digitatus infection relies on morphological examination of either eggs in faecal samples or l3 larvae developed in vitro. technical limitations hinder the practicability of these approaches. hence, in order to develop a specific diagnostic measure for m. digitatus infection, we determined the sequence of the internal transcribed spacer (its) of its ribosomal dna (rdna) and designed primers for pcr-based species-specific amplification of the its to differen ...200616679725
the life cycle of mecistocirrus digitatus, a trichostrongylid parasite of ruminants. 196514275199
morphology, systematics, and geographic distribution of mecistocirrus digitatus, a trichostrongylid parasite of ruminants. 196514275198
development of immunity to mecistocirrus digitatus (nematoda: trichostrongylidae) in calves.the importance of the third (l3), fourth (l4) and adult stages of mecistocirrus digitatus in inducing resistance to reinfection was examined. three groups of 5 calves (a, b, c) were immunized for 2 consecutive days with a daily dose of 20,000 m. digitatus infectious larvae. group a was treated with ivermectin 7 days post-infection (exposed to l3), group b was treated after 26 days (exposed to l3 and l4) and group c after 60 days (exposed to l3, l4 and adults). thirty days posttreatment, animals ...19989695103
epidemiology of mecistocirrus digitatus and other gastrointestinal nematode infections in cattle in mindanao, philippines.over a period of 14 months, faecal and serum samples were collected monthly from cattle of 1 to 30 months old on three ranches (20-25 animals ranch-1 sample-1) in mindanao, philippines. individual faecal egg counts, serum pepsinogen levels and mecistocirrus digitatus specific antibodies were determined, and infective larvae from pooled faecal samples were examined. post mortem worm counts were carried out on tracer calves (n = 16) that had been kept for 30 days with the herds on two of the ranch ...19989493308
sublateral hypodermal chords in haemonchus (nematoda: trichostrongyloidea): description and potential as a systematic character.prominent sublateral hypodermal chords (shc) are described in haemonchus spp. four large shc are located 1 muscle cell away from the lateral chords from the level of the middle of the esophagus to midbody. the shc are largest in midbody and merge with the lateral chords at about 65% of the body length from the anterior end. with the aid of transmission electron microscopy, a band of nerve tissue was located parallel and medial to the shc. haemonchus spp. and mecistocirrus digitatus have shc that ...19948064530
seasonal dynamics in the incidence of mecistocirrus digitatus--(von linstow--1906), a parasite of the abomasum of ruminants. 19665974801
studies on the migratory habits of the infective larvae of mecistocirrus digitatus (von linstow-1906). 19665940397
effect of temperature on the eggs and the larvae of mecistocirrus--digitatus-(von linstow-1906). 19665931154
effects of desiccation on the eggs and the larvae of mecistocirrus digitatus (von linstow-1906). 19655893570
bionomics of the free living stages of mecistocirrus digitatus--(von linstow--1906), a commonly occurring trichostrongyloid worm of cattle and buffaloes. 19655892244
[mecistocirrus digitatus von linstow, 1906, the parasite of cattle of guadeloupe]. 19744480453
immunological studies of mecistocirrus digitatus infection in calves. 19705499306
[study of the inhibitory action of phenothiazine on carbohydrate metabolism in mecistocirrus digitatus (nematoda: rhabditida)].the presence of phenothiazine in incubation medium of m. digitatus exerted the following effects: the increase of glycogene splitting and lactic acid excretion in aerobic conditions; the increase of acetic acid excretion and the decrease of alpha-methylbutyric acid excretion; glycolysis process inhibition in homogenates and supernatants during incubation with glucose. however, at phenothiazine incubation with fdf glycolysis intensity was unaffected.1976958716
proteomic analysis of mecistocirrus digitatus and haemonchus contortus intestinal protein extracts and subsequent efficacy testing in a vaccine trial.gastrointestinal nematode infections, such as haemonchus contortus and mecistocirrus digitatus, are ranked in the top twenty diseases affecting small-holder farmers' livestock, yet research into m. digitatus, which infects cattle and buffalo in asia is limited. intestine-derived native protein vaccines are effective against haemonchus, yet the protective efficacy of intestine-derived m. digitatus proteins has yet to be determined.201424901227
relative importance of larval and adult mecistocirrus digitatus in inducing resistance to a reinfection in calves.calves immunized with adult mecistocirrus digitatus implanted directly into the abomasum did not develop a substantial degree of immunity to a subsequent large oral (challenge) dose of larvae, which developed to maturity. in contrast animals immunized by oral infection developed strong resistance. the calves implanted with adult worms appeared to show a greater degree of susceptibility to maturation of the challenge infection than controls which received a challenge of the same magnitude without ...19873105161
epidemiology of abomasal nematodes of dairy cattle in hokkaido, northern japan.the prevalence and intensity of infection with abomasal nematodiasis was studied in dairy cattle of hokkaido, northern japan, for successive two years. during the period of march in 1985 to september in 1987, a total number of 393 abomasa of holstein-friesian cows was examined for nematode parasites. nematodes were detected from 75% of the cows. the prevalence of nematode species detected was ostertagia ostertagi 250 (63.6%), mecistocirrus digitatus 181 (46.1%), trichostrongylus axei 85 (21.6%) ...19902348599
efficacy of ivermectin against the abomasal nematode mecistocirrus digitatus in naturally infected zebu calves.anthelmintic efficacy of ivermectin against mecistocirrus digitatus was evaluated in 10 naturally infected zebu calves. group 1 (n = 5) was not treated, and group 2 (n = 5) was treated sc with 200 micrograms of ivermectin/kg of body weight. calves were euthanatized 14 or 15 days after treatment. there was 100% overall reduction of m digitatus in treated calves, which was significant (p = 0.0079) compared with reduction in controls. cooperia punctata, haemonchus similis, and trichostrongylus axei ...19873434907
development and validation of real-time pcr for rapid detection of mecistocirrus digitatus.hematophagous activity of mecistocirrus digitatus, which causes substantial blood and weight loss in large ruminants, is an emerging challenge due to the economic loss it brings to the livestock industry. infected animals are treated with anthelmintic drugs, based on the identification of helminth species and the severity of infection; however, traditional methods such as microscopic identification and the counting of eggs for diagnosis and determination of level of infection are laborious, cumb ...201323646171
[characteristics of lipids from the nematode mecistocirrus digitatus]. 19744831016
reappearance of mecistocirrus digitatus in cattle from the mexican tropics: prevalence, molecular, and scanning electron microscopy identification.mecistocirrus digitatus is a hematophagous abomasal nematode which causes significant blood and production losses in cattle. the objectives of the present study were to: (1) report the reappearance of m. digitatus in cattle from the mexican tropics using microscopy, scanning electron microscopy (sem), and molecular identification, and (2) determine the prevalence of m. digitatus in slaughtered adult cattle from the mexican tropics. gastroinestinal nematodes (gin) were recovered from the abomasum ...201424433062
[oxidative processes in tissues of the nematode mecistocirrus digitatus].the tissues of the nematode m. digitatus were used to obtain mitochondria which swell under the effect of several factors. intensive oxidation of succinate, fumarate, malate, oxalacetate, alpha-ketoglutarate and less intensive one -- of citrate, isocitrate and pyruvate were observed in the mitochondria; cis-aconitate was not oxidized at all. nad and nadp increased the intensity of substrate oxidation, whereas parachlormercuribenzoate, malonate and benzoate decreased the latter.1976983566
the significance of the gastrointestinal parasites of asian buffalo in sri lanka.buffalo aged 3-15 months on institutional farms and in the villages of an irrigation project were monitored for gastrointestinal nematodes by faecal egg counts over two years. apart from treatment for toxocara vitulorum at 10-16 days of age, no anthelmintics were used. half of the strongylid egg counts were zero and 90% were less than 500 epg of faeces on the institutional farms, and 67% were zero and 97% less than 500 epg in the villages. no problems arose during four years without anthelmintic ...19902284708
molecular identification of mecistocirrus digitatus and toxocara vitulorum in the mithun (bos frontalis) from north-east india.mecistocirrus digitatus and toxocara vitulorum are common pathogenic nematode parasites of mithun (bos frontalis). species identification by morphological features was confirmed by molecular identification of m. digitatus and t. vitulorum. the internal transcribed spacer-2 (its-2) region and beta tubulin gene of m. digitatus were polymerase chain reaction (pcr) amplified and sequenced. its-2 sequence analysis showed 100% homology with other isolates of m. digitatus and 83% identity with haemonch ...201728848270
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