pseudomonas psychrotolerans sp. nov.three yellow-pigmented, gram-negative, rod-shaped, non-spore-forming bacterial strains, c36t, c37 and c39, were isolated in the medical clinic for small animals and ungulates at the university for veterinary medicine in vienna, austria. on the basis of 16s rrna gene sequence similarity, strain c36t was shown to belong to the genus pseudomonas; pseudomonas oleovorans dsm 1045t was the nearest relative (99.5 % sequence similarity). other pseudomonas species shared <97 % sequence similarity with st ...200415388721
draft genome sequence of pseudomonas psychrotolerans l19, isolated from copper alloy coins.we report the draft genome sequence of pseudomonas psychrotolerans strain l19, isolated from a european 50-cent copper alloy coin. multiple genes potentially involved in copper resistance were identified; however, it is unknown if these copper ion resistance determinants contribute to prolonged survival of this strain on dry metallic copper.201222374955
expression, characterization of a novel nitrilase ppl19 from pseudomonas psychrotolerans with s-selectivity toward mandelonitrile present in active inclusion identify a novel nitrilase with s-selectivity toward mandelonitrile that can produce (s)-mandelic acid in one step.201626564406
two cases with bacteremia suspected to be due to relatively rare pseudomonas (flavimonas) oryzihabitans.pseudomonas oryzihabitans (formerly flavimonas oryzihabitans) is a glucose non-fermentative, gram-negative bacillus which is rarely isolated from human specimens. when isolated, it is on very rare occasion as a causative pathogen of catheter-related bloodstream infection in an immunocompromised patient. herein, we describe two hematological malignancy patients suspected to have p. oryzihabitans bacteremia. we also review cases with bacteremia due to this pathogen and its microbiological characte ...201526184853
phytohormone production endowed with antagonistic potential and plant growth promoting abilities of culturable endophytic bacteria isolated from clerodendrum colebrookianum this study, culturable endophytic bacterial isolates obtained from an ethnomedicinal plant clerodendrum colebrookianum walp., were assessed for their diversity, in vitro screening for their plant growth promoting (pgp) activities and to use them as inoculant for in vivo pgp activities with biocontrol potential. totally, 73 isolates were recovered from different tissues of c. colebrookianum were identified by 16s rrna gene sequencing and phylogenetically analyzed by using box-pcr fingerprintin ...201627825487
unveiling the pan-genome of the sxt/r391 family of ices: molecular characterisation of new variable regions of sxt/r391-like ices detected in pseudoalteromonas sp. and vibrio scophthalmi.integrating conjugative elements (ices) of the sxt/r391 family have been identified in fish-isolated bacterial strains collected from marine aquaculture environments of the northwestern iberian peninsula. here we analysed the variable regions of two ices, one preliminarily characterised in a previous study (icevscspa3) and one newly identified (icepspspa1). bacterial strains harboring these ices were phylogenetically assigned to vibrio scophthalmi and pseudoalteromonas sp., thus constituting the ...201627230650
directed evolution of nitrilase ppl19 from pseudomonas psychrotolerans l19 and identification of enantiocomplementary mutants toward mandelonitrile.nitrilase ppl19 from pseudomonas psychrotolerans l19 can hydrolyze racemic mandelonitrile to (s)-mandelic acid with an enantiomeric excess (ee) value of 52.7%. in this study, random mutagenesis combined with site-directed mutagenesis was performed to identify the key residues responsible for nitrilase enantioselectivity. five enzyme mutants exhibiting distinct selectivity were generated and four "hot spots" (m113, r128, a136, and i168) responsible for enantioselectivity toward mandelonitrile wer ...201526577409
enzymatic characterization and gene identification of aconitate isomerase, an enzyme involved in assimilation of trans-aconitic acid, from pseudomonas sp. wu-0701.trans-aconitic acid is an unsaturated organic acid that is present in some plants such as soybean and wheat; however, it remains unclear how trans-aconitic acid is degraded and/or assimilated by living cells in nature. from soil, we isolated pseudomonas sp. wu-0701 assimilating trans-aconitic acid as a sole carbon source. in the cell-free extract of pseudomonas sp. wu-0701, aconitate isomerase (ai; ec activity was detected. therefore, it seems likely that strain pseudomonas sp. wu-0701 ...201526293748
genomic and phenotypic analyses of pseudomonas psychrotolerans prs08-11306 reveal a turnerbactin biosynthesis gene cluster that contributes to nitrogen fixation.plant-microbe interactions can provide agronomic benefits, such as enhancing nutrient uptake and providing fixed nitrogen. the pseudomonas psychrotolerans strain prs08-11306 was isolated from rice seeds and can enhance plant growth. here, we analyzed the p. psychrotolerans genome, which is ∼5mb, with 4389 coding sequences, 77 trnas, and 7 rrnas. genome analysis identified a cluster of turnerbactin biosynthetic genes, which are responsible for the production of a catecholate siderophore and contr ...201728528782
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