| ssg, a putative glycosyltransferase, functions in lipo- and exopolysaccharide biosynthesis and cell surface-related properties in pseudomonas alkylphenolia. | in the presence of vaporized p-cresol, pseudomonas alkylphenolia kl28 forms specialized aerial structures (sas). a transposon mutant of strain kl28 (c23) incapable of forming mature sas was isolated. genetic analysis of the c23 mutant revealed the transposon insertion in a gene (ssg) encoding a putative glycosyltransferase, which is homologous to the pseudomonas aeruginosa pao1 pa5001 gene. deletion of ssg in kl28 caused the loss of lipopolysaccharide o antigen and altered the composition of the ... | 2010 | 21166709 |
| Identification of a p-Cresol Degradation Pathway by a GFP-Based Transposon in Pseudomonas and Its Dominant Expression in Colonies. | In this study, the chromosome-encoded pcuRCAXB genes that are required for p-cresol degradation have been identified by using a newly constructed green fluorescent protein (GFP)-based promoter probe transposon in the long-chain alkylphenol degrader Pseudomonas alkylphenolia. The deduced amino acid sequences of the genes showed the highest identities at the levels of 65-93% compared with those in the databases. The transposon was identified to be inserted in the pcuA gene, with the promoterless g ... | 2011 | 22127130 |
| an alginate-like exopolysaccharide biosynthesis gene cluster involved in biofilm aerial structure formation by pseudomonas alkylphenolia. | pseudomonas alkylphenolia is known to form different types of multicellular structures depending on the environmental stimuli. aerial structures formed during vapor p-cresol utilization are unique. transposon mutants that showed a smooth colony phenotype failed to form a differentiated biofilm, including aerial structures and pellicles, and showed deficient surface spreading motility. the transposon insertion sites were located to a gene cluster designated epm (extracellular polymer matrix), whi ... | 2014 | 24493568 |
| complete genome sequence of the mushroom-like aerial structure-forming pseudomonas alkylphenolia, a platform bacterium for mass production of poly-β-d-mannuronates. | pseudomonas alkylphenolia (kctc 22206, jcm 16553) metabolizes phenols with an alkyl chain (c1-c5) at the para position. this strain forms mushroom-like aerial structures under p-cresol vapor, and its mutants are exploited to augment the production of poly-β-d-mannuronate and its derivatives. in this study, we report the 5.7mbp complete genome sequence of p. alkylphenolia, which will provide a basis for biotechnological applications of this strain. | 2014 | 25300744 |