| the morphometrics of babesia motasi (wales) and its transmission by haemaphysalis punctata (canestrini and fanzago 1877) to sheep. | babesia motasi (wales) was transmitted to sheep by larvae, nymphs and adult female haemaphysalis punctata ticks which were either collected from rough grazing pasture, at mynydd mawr, aberdaron, north wales, or from laboratory cultures derived from ticks collected at the above site. both transovarial and transstadial transmission of infection were demonstrated. only larvae were shown to pick up infection. the parasite could not be demonstrated in intact sheep, either in blood films or following ... | 1988 | 3245109 |
| serodiagnosis of babesia motasi (wales), theileria recondita (wales) and cytoecetes phagocytophila infection in sheep. | the indirect fluorescent antibody test (ifat) was used to diagnose some tick-borne infections of sheep, babesia motasi (wales), theileria recondita (wales) and cytoecetes phagocytophila. antigen was prepared from blood derived from splenectomised sheep except for c phagocytophila which was derived from a normal animal. a field survey was made to assess the prevalence of b motasi and t recondita in north wales and a comparison made between the titres using the b motasi (wales) antigen with those ... | 1987 | 3306850 |
| the pathogenesis of babesia motasi (wales) infection in sheep. | studies on the pathogenesis of babesia motasi (wales) infection following blood transfusion of infected blood to normal or splenectomised recipients showed that the intact animal is refractory to infection but that infection in splenectomised animals caused weight loss, fever, anorexia, lassitude and a macrocytic hypochromic anaemia which coincided with the peak of parasitaemia. there was an initial leucocytosis, largely due to a neutrophilia. the prepatent period following blood transfusion was ... | 1988 | 3369074 |
| some observations on babesia motasi infection in sheep in andhra pradesh. | | 1966 | 5950635 |
| babesia motasi in sheep on the island of gotland in sweden. | serological survey with an ifa-test showed that 58% (73/125) of the lambs and 88% (46/52) of the ewes on the island of gotland (sweden) has antibodies against babesia motasi. it is likely that such a high incidence of the blood protozoan organisms plays an important part in causing the often observed anaemia in sheep on that island. | 1981 | 7046205 |
| investigations on babesia motasi isolated from wales. | a number of observations were carried out on the effect of the newly isolated parasite, babesia motasi (wales) on intact and splenectomised sheep and on a splenectomised goat. the parasite was not pathogenic for intact animals but caused fever and anaemia in splenectomised ones. b motasi (wales) was also studied morphologically, morphometrically and serologically. the common form of the parasite was seen to be a double pyriform, the mean length of one side being 2.23 micrometer. it appeared to b ... | 1981 | 7323470 |
| blood parasites of sheep in the netherlands. ii. babesia motasi (sporozoa, babesiidae). | a large babesia species occurs in sheep on the north sea islands of the netherlands. the tick haemaphysalis punctata is a vector. its pathogenicity appears to be low. it is morphologically similar to a turkish strain, considered to be b. motasi, which is also transmitted by haemaphysalis ticks. it differs from the turkish parasite serologically as well as in cross-immunity tests and in not being effective to goats. there may be a group of morphologically similar parasites with serological differ ... | 1980 | 7352333 |
| piroplasms of domestic animals in the macedonia region of greece. 3. piroplasms of small ruminants. | a study was carried out on piroplasms of small ruminants in the macedonia region of greece. during 1984-1985, 721 serum samples were collected from sheep in 49 localities and 487 from goats in 43 localities. blood smears were also prepared from 26 sheep and eight goats in 16 localities. the prevalence of positive ifa titres for sheep and goat sera, respectively, was: 24.6% and 0.6% for theileria ovis, 52.1% and 36.4% for babesia ovis, 10.5% and 4.2% for babesia motasi, 12.6% and 6.6% for babesia ... | 1996 | 8792581 |
| the indirect fluorescent antibody test based on schizont antigen for study of the sheep parasite theileria lestoquardi. | an indirect fluorescent antibody test (ifat), based on schizont-infected lymphoblastoid cells, was applied to study the course of antibody production in adult sheep inoculated with attenuated, in vitro grown, theileria lestoquardi (theileria hirci) infected cells. bright fluorescence of the intracellular schizonts could first be demonstrated 15 days after inoculation. a 32-64-fold rise in antibody titres was recorded 1 month after infection, and substantial titres were still observed 90 days aft ... | 1997 | 9187025 |
| general review of tick species present in portugal. | at present, 24 species are known to occur in portugal: argas vespertilionis, ornithodoros maritimus and ornithodoros erraticus in argasidae; ixodes acuminatus, ixodes bivari, ixodes canisuga, ixodes frontalis, ixodes hexagonus, ixodes ricinus, ixodes simplex simplex, ixodes ventalloi, ixodes vespertilionis, dermacentor marginatus, dermacentor pictus', haemaphysalis hispanica, haemaphysalis inermis, haemaphysalis punctata, rhipicephalus bursa, rhipicephalus pusillus, rhipicephalus sanguineus, rhi ... | 1999 | 11071535 |
| an epidemiological study on ovine babesiosis in the mashhad suburb area, province of khorasan, iran. | the prevalence of babesia spp. infection was studied in sheep of the mashhad area in iran from 1998 to 2000. a total of 677 sheep originating from 115 flocks were clinically examined and investigated for the presence of babesia spp. in appropriate blood smears and any tick species on the body of the animals. the study revealed that the infection rate for babesia ovis and babesia motasi were 167 (24.6%) and 4 (0.5%), respectively. double (mixed) infections occurred in 21 (3%) sheep. differences i ... | 2002 | 12208039 |
| [on the host specificity of babesia motasi and babesia ovis (piroplasmidea)]. | | 1964 | 14221622 |
| identification, genetic diversity and prevalence of theileria and babesia species in a sheep population from northern spain. | the genetic diversity and prevalence of virtually all theileria and babesia species in a sheep population were studied using a specifically designed reverse line blot macroarray. the amplified hypervariable v4 region of the 18s rrna gene was hybridised against generic and species-specific probes. in a first screening (study i), 320 apparently healthy animals corresponding to 32 flocks located in the basque country (northern spain) were analysed. the survey demonstrated a high prevalence of subcl ... | 2004 | 15313132 |
| development of a polymerase chain reaction method for diagnosis of babesia ovis infection in sheep and goats. | in this study, a pair of oligonucleotide primers were designed according to the nucleotide sequence of the small subunit ribosomal rna (ssu rrna) gene of babesia ovis isolated from sheep in eastern turkey. the primers were used to detect parasite dna from blood samples of b. ovis-infected sheep and goats by polymerase chain reaction (pcr). a 549-bp dna fragment was specifically amplified from blood samples from sheep and goats, naturally infected with b. ovis. no pcr products resulted from babes ... | 2005 | 16043298 |
| determination of rhipicephalus spp. as vectors for babesia ovis in iran. | the tick-borne diseases of livestock constitute a complex of several diseases with different etiological agents, such as protozoa, rickettsia, bacteria, and viruses. one problem discussed in protozoan infection is the determination and characterization of the transmitter agent. because many analyses were performed with the salivary gland smears using the methyl-green-pyronin staining method or the feulgen staining method, the transfer vector remains unanswered in some cases. the aim of this stud ... | 2007 | 17554562 |
| ticks of small ruminants in china. | the importance of ticks and tick-borne diseases of small ruminants in china is discussed. of the 109 species of ticks identified to date in china, 45 species infest small ruminants. five species have been proved to be involved, or possibly involved, in the transmission of tick-borne diseases. anaplasma ovis, babesia motasi, babesia ovis and two unidentified species of theileria, have been recorded in small ruminants in china. the diseases caused by these organisms are widespread in china, causin ... | 2007 | 17823826 |
| investigations into the natural infection rate of haemaphysalis qinghaiensis with piroplasma using a nested pcr. | here we describe the natural infection rate in china of haemaphysalis qinghaiensis with four piroplasma species, namely theileria uilenbergi, t. luwenshuni, t. sinensis and babesia motasi. specifically, a nested pcr was designed based on 18s ribosomal rna genes and its specificity and sensitivity were established. the result showed that 62 flat adult field h. qinghaiensis ticks (27 females and 35 males) out of 136 (55 females and 81 males) were infected by one or more parasites. all 62 (45.6%) w ... | 2008 | 18273685 |
| biometrical and genetical characterization of large babesia ovis in iran. | one species of babesia was identified on the blood smear of 20 different naturally infected sheep in the northwest of iran. it was polymorphic, including double pyriform with acute or obtuse angle, single pyriform, and ring form. the size of typical paired pyriforms with acute angle was 2.7 x 0.4 microm (n=10) and with obtuse angle was 3.5 x 0.6 microm (n=10). although the morphological and biometrical parameters resembled the babesia motasi, the results of seminested polymerase chain reaction ( ... | 2008 | 18386062 |
| differentiation of two ovine babesia based on the ribosomal dna internal transcribed spacer (its) sequences. | the first and second internal transcribed spacers (its1, its2) as well as the intervening 5.8s coding region of the rrna gene for six babesia spp. isolated from different geographic origins were characterized. varying degrees of its1 and its2 intra- and inter-species sequence polymorphism were found among these isolates. phylogenetic analysis of the its1-5.8s gene-its2 region clearly separated the isolates into two clusters. one held an unidentified babesia sp. transmitted by hyalomma anatolicum ... | 2009 | 18977349 |
| small-scale expressed sequence tag analysis of theileria uilenbergi: identification of a gene family encoding potential antigenic proteins. | recently, theileria sp. (china) has been designated as t. luwenshuni[formerly theileria sp. (china 1)] and t. uilenbergi[formerly theileria sp. (china 2)]. a cdna library of t. uilenbergi merozoites was constructed and subjected to random sequencing. among the obtained sequences were three highly identical cdna clones, indicating a gene family. bioinformatic analyses indicated these genes contain signal peptides and encode potential immunogenic proteins. the presence of tandemly arranged and add ... | 2008 | 19120214 |
| detection and differentiation of ovine theileria and babesia by reverse line blotting in china. | a reverse line blot (rlb) assay was developed for detection and specific identification of the different ovine theileria and babesia parasites. in a polymerase chain reaction (pcr), the hypervariable region 4 (v4 region) of the 18s ribosomal dna gene was amplified with a set of general primers specific for members of the genera theileria and babesia. meanwhile, specific oligonucleotide probes were designed and bound on membrane. after one single-pcr amplification, the amplified fragment was hybr ... | 2009 | 19205742 |
| blood parasites of sheep in the netherlands. ii. babesia motasi (sporozoa, babesiidae). | summary a large babesia species occurs in sheep on the north sea islands of the netherlands. the tick haemaphysalis punctata is a vector. its pathogenicity appears to be low. it is morphologically similar to a turkish strain, considered to be b. motasi, which is also transmitted by haemaphysalis ticks. it differs from the turkish parasite serologically as well as in cross-immunity tests and in not being effective to goats. there may be a group of morphologically similar parasites with ser ... | 1980 | 22039853 |
| Babesia ovis as the main causative agent of sheep babesiosis in Iran. | Babesiosis is a haemoparasitic disease with high economical losses in livestock industry worldwide. The early diagnosis and successful therapy of babesiosis belong to the key steps of control and health management of livestock. Ethanol-fixed blood samples of 400 sheep were analyzed for Babesia infection. Reverse line blot (RLB) was established specifically for Theileria lestoquardi, Theileria (China 1), Theileria (China 2), Theileria ovis, Theileria separata, Babesia ovis, Babesia motasi, Babesi ... | 2011 | 21975684 |
| a study on ovine tick-borne hemoprotozoan parasites (theileria and babesia) in the east black sea region of turkey. | in this study, the frequency of theileria and babesia species was assessed via reverse line blotting and blood smear-based diagnostic methods in small ruminants. a total of 201 apparently healthy animals from 26 randomly selected herds located in 4 locations (artvin, giresun, gumushane, and tokat) of east black sea region of turkey were investigated for the blood protozoans. in a polymerase chain reaction (pcr), the hypervariable v4 region of the 18s ribosomal rna gene was amplified with a set o ... | 2012 | 22231266 |
| molecular detection and identification of piroplasms in sika deer (cervus nippon) from jilin province, china. | piroplasmosis is an important disease of domestic animals and wildlife and is caused by organisms from the genera theileria and babesia. wildlife such as sika deer play an important role as reservoir hosts for several species of theileria and babesia. using blood samples collected from sika deer, we investigated the epidemiology of theileria spp. and babesia spp. in sika deer from jilin province in china and identified those species that cause pathogenic infections in sika deer. | 2016 | 26984286 |
| blood parasites of livestock in certain regions in saudi arabia. | blood samples from camels, sheep, goats and cattle from six regions in saudi arabia were examined for blood parasites. asir region camels were disinfected while those of the eastern, jazan, northern frontiers, riyadh and tabouk regions were infected with trypanosoma evansi (5-40%), those of riyadh and the eastern regions were infected with dipetalonema evansi (1-6%) and those of the eastern, jazan and riyadh regions were infected with eperythrozoon species (8-20%). sheep and goats of all tested ... | 0 | 23961044 |
| assessment of exposure to piroplasms in sheep grazing in communal mountain pastures by using a multiplex dna bead-based suspension array. | piroplasms are tick-borne hemoprotozoans with a major impact on extensive management systems. detection of sub-clinical low-level carriers, which can act as source of infection for vector ticks, is key to protect livestock trade and facilitate preventive control programs. the purpose of this study was to develop a method for the detection of ovine piroplasms and to use it in a field study aimed at investigating piroplasms infection in semi-extensive production systems in the basque country (nort ... | 2013 | 24499621 |
| expression analysis and biological characterization of babesia sp. bq1 (lintan) (babesia motasi-like) rhoptry-associated protein 1 and its potential use in serodiagnosis via elisa. | in china, ovine babesiosis is one of the most important tick-borne haemoparasitic diseases of small ruminants. it has a significant economic impact, and several babesia motasi-like isolates have been recently shown to be responsible for ovine babesiosis in this country. | 2016 | 27245213 |
| development of an immunochromatographic strip for the serodiagnosis of theileria infection in sheep. | theileria uilenbergi and t. luwenshuni are tick-borne protozoan parasites, transmitted by haemaphysalis qinghaiensis and h. longicornis, respectively. they are the main causative agents of theileriosis in small ruminants in china. the disease has resulted in severe economic losses and hindered the development of sheep and goat husbandry industry in the endemic regions. | 2015 | 26631226 |
| a rapid, simple and sensitive loop-mediated isothermal amplification method to detect anaplasma bovis in sheep and goats samples. | a loop-mediated isothermal amplification (lamp) technique has been widely used in detecting the nucleic acid of various pathogenic bacteria. in this study, a set of four lamp primers was designed to specifically test anaplasma bovis. the lamp assay was performed at 62°c for 60min in a water bath. the specificity was confirmed by amplifying a. bovis isolate, while no cross reaction was observed with other five pathogens (anaplasma bovis, anaplasma phagocytophilum, theileria luwenshuni, babesia mo ... | 2017 | 28351721 |
| a loop-mediated isothermal amplification (lamp) assay targeting 16s rrna gene for rapid detection of anaplasma phagocytophilum infection in sheep and goats. | anaplasma phagocytophilum is a zoonotic pathogen and the causative agent of human granulocytic anaplasmosis (hga) in humans and tick-borne fever in various kinds of animals. in the present study, a loop-mediated isothermal amplification (lamp) assay for rapid detection of a. phagocytophilum was developed using primers specific to 16s rrna gene of this organism. the lamp assay was performed at 65 c for 60 min and terminated at 80 c for 10 min. the optimal reaction conditions, under which no cross ... | 2017 | 28118097 |
| evaluating an indirect rmpsp enzyme-linked immunosorbent assay for the detection of bovine theileria infection in china. | bovine theileriosis, a tick-borne protozoan disease caused by theileria annulata, theileria orientalis and theileria sinensis, is widespread in china and is a serious economic problem for the chinese livestock industry. in this study, recombinant major piroplasma surface proteins (mpsp) of t. annulata, t. orientalis and t. sinensis based on mpsp genes were expressed in escherichia coli bl21(de3). the immunogenicity and specificity of the three purified recombinant mpsp proteins were evaluated wi ... | 2017 | 27942962 |
| theileria sp. ot3 and other tick-borne pathogens in sheep and ticks in italy: molecular characterization and phylogeny. | pcr reverse line blot (rlb) hybridization and sequencing were used to determine the dynamics of infection with tick-borne pathogens in one hundred apparently healthy sheep in italy. blood samples were tested once prior to the onset of the grazing season (june 2010) and once after the end of the grazing season (august 2010). ticks collected from sheep and from the vegetation were also tested by pcr/rlb. before grazing, 56% of the sheep harbored several tick-borne pathogens: anaplasma ovis was the ... | 2015 | 25448422 |
| genetic diversity and molecular characterization of babesia motasi-like in small ruminants and ixodid ticks from china. | ovine babesioses, an important tick-borne disease of sheep and goats in china, is caused by the reproduction of intraerythrocytic protozoa of the babesia genus. babesia motasi-like is a babesia parasite that infects small ruminant in china, and two sub-groups of b. motasi-like can be subdivided based on differences in the rhoptry-associated-protein-1 gene. this study aimed to characterize the distribution, epidemiology and genetics of b. motasi-like in animals and ticks. a molecular investigatio ... | 2016 | 26976477 |
| a member of the hsp90 family from ovine babesia in china: molecular characterization, phylogenetic analysis and antigenicity. | heat shock protein 90 (hsp90) is a key component of the molecular chaperone complex essential for activating many signalling proteins involved in the development and progression of pathogenic cellular transformation. a hsp90 gene (bqhsp90) was cloned and characterized from babesia sp. bq1 (lintan), an ovine babesia isolate belonging to babesia motasi-like group, by screening a cdna expression library and performing rapid amplification of cdna ends. the full-length cdna of bqhsp90 is 2399 bp with ... | 2015 | 26156495 |
| strong conservation of rhoptry-associated-protein-1 (rap-1) locus organization and sequence among babesia isolates infecting sheep from china (babesia motasi-like phylogenetic group). | rhoptry-associated-protein 1 (rap-1) is considered as a potential vaccine candidate due to its involvement in red blood cell invasion by parasites in the genus babesia. we examined its value as a vaccine candidate by studying rap-1 conservation in isolates of babesia sp. bq1 ningxian, babesia sp. tianzhu and babesia sp. hebei, responsible for ovine babesiosis in different regions of china. the rap-1 locus in these isolates has very similar features to those described for babesia sp. bq1 lintan, ... | 2014 | 25200723 |
| sequence and organization of the rhoptry-associated-protein-1 (rap-1) locus for the sheep hemoprotozoan babesia sp. bq1 lintan (b. motasi phylogenetic group). | babesiosis is a frequent infection of animals worldwide by tick borne pathogen babesia, and several species are responsible for ovine babesiosis. recently, several babesia motasi-like isolates were described in sheep in china. in this study, we sequenced the multigenic rap-1 gene locus of one of these isolates, babesia sp. bq1 lintan. the rap-1 proteins are involved in the process of red blood cells invasion and thus represent a potential target for vaccine development. a complex composition and ... | 2013 | 24075419 |
| a sero-epidemiological survey of chinese babesia motasi for small ruminants in china. | babesia motasi bq1 (lintan) was first isolated from haemaphysalis qinghaiensis collected in gannan tibet autonomous region, gansu province in april 2000. in this study, a total of 3,204 serum samples from small ruminants in 22 provinces located in different districts of china were tested for antibodies against merozoite antigens from cultured b. motasi bq1 (lintan) by enzyme-linked immunosorbent assay. this method can survey the prevalence of low-pathogenic chinese b. motasi. the results of this ... | 2013 | 23371500 |
| molecular detection of theileria spp. and babesia spp. in sheep and ixodid ticks from the northeast of iran. | theilerioses and babesioses are important diseases in iranian sheep. the present study was undertaken to identify and classify/specify theileria spp. and babesia spp. in sheep and vector ticks. investigation was carried out from 2009 to 2011 in the khorasan razavi province, iran. in total, 302 sheep originating from 60 different flocks were clinically examined and their blood collected. in addition, from the same flocks, ixodid ticks were sampled. stained blood smears were microscopically examin ... | 2013 | 22924924 |
| prevalence of tick-borne haemoparasites in small ruminants in turkey and diagnostic sensitivity of single-pcr and rlb. | tick-borne haemoparasitic diseases (tbhds), caused by theileria, babesia, anaplasma and ehrlichia, are common in regions of the world where the distributions of host, pathogen and vector overlap. many of these diseases threaten livestock production and some also represent a concern to human public health. the primary aim of this study was to determine the prevalence of the above-mentioned pathogens in a large number of blood samples (n = 1979) collected from sheep (n = 1727) and goats (n = 252) ... | 2017 | 28449722 |