| eperythrozoon ovis. i. serological diagnosis of infection by the indirect immunoflurescent antibody test. | the indirect immunofluorescent antibody test (ifat) was evaluated for the detection of eperythrozoon ovis infection in sheep. the test first became positive, on the average, 10 days following exposure to e. ovis and remained positive for 24 months, the longest period checked. reciprocal titres in the first three days after becoming positive in the ifat were between 4 and 80, but rose to 640 within 9 weeks; in general titres were between 4 and 640, exceptionally titres of 1280 were obtained. nega ... | 1978 | 83044 |
| electron-microscopic studies on theileria ovis rodhain, 1916: development of kinetics in the gut of the vector tick, rhipicephalus evertsi evertsi neumann, 1897, and their transformation within cells of the salivary glands. | gamogony of theileria ovis rodhain occurs within the gut of nymphs of the tick rhipicephalus evertsi evertsi neumann. after molting, spherical and ovoid parasites are found within the intestinal cells of the ticks. these stages are thought to be zygotes, because they undergo a transformation process leading ultimately (in 3 days) to the formation of a motile stage, the kinete, the fine structure of which is very similar to that of the ookinetes of the hemosporidia. the kinete leaves the gut cell ... | 1979 | 119849 |
| observations on haematoxenus separatus uilenberg & andreasen 1974 in the erythrocytes of kenyan sheep. | two kenyan sheep were splenectomised; on examination of giemsa stained blood smears large numbers of piroplasms identical in morphology to theileria ovis and haematoxenus separatus (with a veil in the infected erythrocyte) were detected. uncoagulated infected blood was examined under phase-contrast and it was found that the vast majority of the infected erythrocytes had veils. the veils were more resistant to lysis than the erythrocyte itself and in lysed preparations the veils were seen floatin ... | 1977 | 415344 |
| isolation and identification of babesia ovis in extremadura (spain). | a morphological and morphometric comparison was made of a babesia ovis strain isolated in extremadura (spain) and a b. ovis strain from turkey. strains were morphologically similar, with a clear predominance of single sub-spherical or anaplasmoid forms. the use of an image analyser to measure b. ovis merozoites revealed significant differences in the long axis, short axis and volume between the two strains. both strains however behaved in a similar manner in the indirect fluorescent antibody (if ... | 1990 | 1693020 |
| [ticks and hemoparasitic diseases in cattle in senegal. iv. the southern sudan area]. | the authors report on the results of an investigation on ticks and hemoparasitoses of cattle, sheep and goats in the south sudanian area of senegal. systematic routine dipping against ticks of cattle, 40 sheep and 40 goats was set during 15 months, with a view to determine the population dynamics together with an acurate localization of the different species concerned. the following parasites were collected from these ruminants: amblyomma variegatum, boophilus geigyi, hyalomma truncatum, h. m. r ... | 1990 | 2218038 |
| the significance of eperythrozoon ovis in ill-thrift in sheep in the eastern cape coastal areas of south africa. | ill-thrift in sheep in the coastal region of the eastern cape is described. it is shown to be a complex problem attributable to many causes, most of which can be eliminated by supplementary feeding, drenching, vaccination, dipping and management. however, studies on the aetiology of ill-thrift in young sheep indicate that arthropod-borne anaemia-producing pathogens are an important contributing factor, which cannot readily be diagnosed and controlled. experiments were conducted on various farms ... | 1988 | 3210217 |
| the experimental transmission of theileria ovis by rhipicephalus evertsi mimeticus and r. bursa. | | 1972 | 4197846 |
| the piroplasm theileria ovis detected in sheep in south wales. | | 1981 | 6789542 |
| attempts to transmit theileria ovis through the ticks haemaphysalis bispinosa and rhipicephalus haemaphysaloides. | | 1980 | 7189305 |
| [ticks and hemoparasitoses of livestock in senegal. vi. the soudano-sahelian zone]. | the authors report the results of a study on ticks and hemoparasitoses among cattle, sheep and goats in the sudano-sahelian zone. during a period of 15 months, ticks were systematically removed from 40 cattle, 40 sheep and 40 goats in order to assess the population dynamics and determine more accurately the preferential sites of settlement of the different species. the following species were collected in ruminants: hyalomma truncatum, h. marginatum rufipes, rhipicephalus evertsi evertsi, rh. gui ... | 1994 | 7991897 |
| [ticks and hemoparasitoses in livestock in senegal. v. the northern guinea area]. | the authors report the results of a study on ticks and hemoparasitoses among cattle, sheep and goats in the north-guinean zone. during a period of 15 months, ticks were systematically removed from 40 cattle, 40 sheep and 40 goats in order to assess the population dynamics and to determine more accurately the preferential sites of settlement of the following different species collected from these animals: amblyomma variegatum, boophilus geigyi, hyalomma truncatum, h. marginatum rufipes, rhipiceph ... | 1993 | 8073170 |
| piroplasms of domestic animals in the macedonia region of greece. 3. piroplasms of small ruminants. | a study was carried out on piroplasms of small ruminants in the macedonia region of greece. during 1984-1985, 721 serum samples were collected from sheep in 49 localities and 487 from goats in 43 localities. blood smears were also prepared from 26 sheep and eight goats in 16 localities. the prevalence of positive ifa titres for sheep and goat sera, respectively, was: 24.6% and 0.6% for theileria ovis, 52.1% and 36.4% for babesia ovis, 10.5% and 4.2% for babesia motasi, 12.6% and 6.6% for babesia ... | 1996 | 8792581 |
| the indirect fluorescent antibody test based on schizont antigen for study of the sheep parasite theileria lestoquardi. | an indirect fluorescent antibody test (ifat), based on schizont-infected lymphoblastoid cells, was applied to study the course of antibody production in adult sheep inoculated with attenuated, in vitro grown, theileria lestoquardi (theileria hirci) infected cells. bright fluorescence of the intracellular schizonts could first be demonstrated 15 days after inoculation. a 32-64-fold rise in antibody titres was recorded 1 month after infection, and substantial titres were still observed 90 days aft ... | 1997 | 9187025 |
| seroprevalence of theileria ovis in small ruminants in north-east spain determined by the indirect fluorescent antibody test. | | 1999 | 10530884 |
| studies on theileria and babesia infecting live and slaughtered animals in al arish and el hasanah, north sinai governorate, egypt. | during the year 2001, a total of 475 sheep, 200 goats, 135 cattle and 190 camels in el arich city and el hassanah center were examined for babesia ovis and theileria ovis. blood films were taken from the vein of the ear. meanwhile, the animals were examined for tick infestations. b. ovis and th. ovis were detected in 13 (2.7%), and 14 (2.9%) sheep, 14 (7.0%), and 15 (7.5%) goats, 13 (9.6%), and 11 (8.1%) cattle and 18 (9.5%), and 24 (12.6%) camels respectively. on the other hand, double infectio ... | 2002 | 12214937 |
| [on the susceptibility of domestic and wild ruminants for theileria ovis (piroplasmidea)]. | | 1964 | 14221618 |
| identification, genetic diversity and prevalence of theileria and babesia species in a sheep population from northern spain. | the genetic diversity and prevalence of virtually all theileria and babesia species in a sheep population were studied using a specifically designed reverse line blot macroarray. the amplified hypervariable v4 region of the 18s rrna gene was hybridised against generic and species-specific probes. in a first screening (study i), 320 apparently healthy animals corresponding to 32 flocks located in the basque country (northern spain) were analysed. the survey demonstrated a high prevalence of subcl ... | 2004 | 15313132 |
| detection of theileria ovis in naturally infected sheep by nested pcr. | a nested polymerase chain reaction (pcr) for the detection of theileria ovis in sheep using oligonucleotide primers designed from the small subunit ribosomal rna (ssu rrna) gene sequence of t. ovis from sheep in eastern turkey is described. a 398-bp dna fragment was specifically amplified from blood samples from sheep, naturally infected with t. ovis. no pcr products resulted from t. lestoquardi, t. annulata, t. parva, t. buffeli and babesia spp. dna using these specific primers. the sensitivity ... | 2005 | 15631901 |
| pcr-based detection of theileria ovis in rhipicephalus bursa adult ticks. | tick-borne diseases in ruminants are common in tropical and subtropical regions and lead to meat and milk production losses. in this study, polymerase chain reaction (pcr) was used to assess the presence of theileria ovis in rhipicephalus bursa ticks. we have demonstrated that the pcr enabled detection of t. ovis in field isolates of r. bursa collected from naturally infested sheep and goats in eastern turkey. the sampling was done in spring season (between may and june 2004). a total of 420 r. ... | 2006 | 16682122 |
| molecular detection and characterization of piroplasms infecting cervids and chamois in northern spain. | wildlife can act as reservoir of different tick-borne pathogens of veterinary and zoonotic importance. to investigate the role of wild ruminants as reservoir of piroplasm infection, 28 red deer, 69 roe deer and 38 chamois from northern spain were examined by reverse line blot (rlb) hybridization. the survey detected a prevalence of 85.7% in red deer, 62.3% in roe deer and 28.9% in chamois. four different piroplasms were identified: theileria sp. ot3 (previously described in sheep) as the most pr ... | 2007 | 17076924 |
| phylogenetics of theileria species in small ruminants. | our study is based on the collection of blood and ticks from sheep in iran and italy. polymerase chain reaction (pcr) testing was performed to target the 18s rrna gene and rlb was performed using previously published probes. in italy and iran 78.7% and 76.0% of the sheep were pcr positive, which after sequencing and rlb showed that they were theileria ovis and theileria lestoquardi, respectively. phylogenetic analysis was performed using the clustal w multiple sequence alignment program and our ... | 2006 | 17135559 |
| [survey of theileria ovis in sheep and goats in the elazig region using the polymerase chain reaction.]. | this study was carried out in order to investigate the presence of theileria ovis in small ruminants in the elazig region between april-october 2004. a total of 164 whole blood and thin blood smears (from 103 sheep and 61 goats) were collected from 15 flocks in different locations. t. ovis piroplasm dna extracted from sheep and goats' blood was amplified by the polymerase chain reaction (pcr) using specific primers. thin blood smears were examined for theileria piroplasms by microscopic examinat ... | 2005 | 17167737 |
| sequence polymorphism in the ribosomal dna internal transcribed spacers differs among theileria species. | the genomic region spanning the two ribosomal rna internal transcribed spacers (its1 and its2) and the 5.8s rrna gene was cloned and sequenced from sixteen theileria isolates. each theileria species possessed its1 and its2 of unique size(s) and species specific nucleotide sequences. varying degrees of its1 and its2 intra- and inter-species sequence polymorphism were found among ruminant theileria species. the spacers were most polymorphic in the agent of tropical theileriosis, theileria annulata ... | 2007 | 17513053 |
| molecular detection of theileria sp. in ticks and naturally infected sheep. | seven healthy sheep and 10 sheep diagnosed with piroplasmosis based on clinical signs were tested for the presence of babesiae and theileriae. using the molecular techniques, two species of theileriae were detected and characterized. theileria ovis was present mostly in healthy sheep and in rhipicephalus ticks collected from infected sheep. theileria sp. ot3 parasite was detected mostly in ill animals which represent additional evidence to the possible pathogenic nature of theileria sp. ot3. the ... | 2008 | 18158215 |
| hemoprotozoa of domestic animals in france: prevalence and molecular characterization. | very limited information is available on epizootiology of haematozoan infections in french domestic animals. in an attempt to address this issue, prevalence of piroplasmida was studied in carnivores and ruminants, whereas prevalence of hepatozoon spp. was only investigated in carnivores. in total, 383 animals were included in the survey (namely 116 cats, 108 dogs, 91 sheep and 68 cows). parasite diagnosis was carried out using molecular methods such as pcr and sequencing of the 18s rrna gene. in ... | 2009 | 19013719 |
| high prevalence of theileria sp. in wild chinese water deer (hydropotes inermis argyropus) in south korea. | wild chinese water deer exist in large numbers in northeast asia, including south korea. the deer population is so widespread that they are common even in urban areas, resulting in increased contact with humans and domestic animals. the purpose of this study was to examine the prevalence of theileria sp. infection in wild chinese water deer in south korea. using biomolecular techniques, blood samples taken from 18 wild chinese water deer were examined. thirteen of the 18 samples (72%) tested pos ... | 2009 | 19577370 |
| experimental transmission of theileria ovis by hyalomma anatolicum anatolicum. | the experimental transmission of a newly isolated theileria ovis is described. hyalomma anatolicum anatolicum nymphs developed from larvae engorged on sheep infected with t. ovis were able to transmit the t. ovis to splenectomized sheep. meanwhile, h. anatolicum anatolicum adults developed from nymphs engorged on sheep infected with t. ovis were also able to transmit t. ovis to splenectomized sheep. these experiments suggested that t. ovis could be transmitted by a h. anatolicum anatolicum, and ... | 2010 | 20165879 |
| theileria ovis discovered in china. | a polymerase chain reaction (pcr) using 989/990 primers was conducted to identify a newly isolated theileria sp. in xinjiang province of china. the target dna fragments of the complete 18s rrna gene were cloned and sequenced. the phylogenetic relationship of newly isolated theileria spp. was inferred based on the 18s rrna gene. the results showed that the new theileria sp. belonged to the cluster of theileria ovis. moreover, the findings were confirmed by t. ovis species-specific pcr. an expecte ... | 2011 | 20619262 |
| identification of different theileria species (theileria lestoquardi, theileria ovis, and theileria annulata) in naturally infected sheep using nested pcr-rflp. | ovine theileriosis is an important hemoprotozoal disease of sheep and goats in tropical and subtropical regions that leads to economic losses in these animals. a nested pcr-restriction fragment length polymorphism (rflp) was carried out to identification theileria species in sheep in some area in western half of iran (sari, rasht, urmia, ilam, and ahvaz). two hundred and fifty blood samples were taken from sheep during tick activating season (summer of 2008). microscopic examination revealed tha ... | 2010 | 20978792 |
| development and evaluation of a loop-mediated isothermal amplification method for rapid detection of anaplasma ovis. | anaplasma ovis is an intraerythrocytic rickettsial pathogen of small ruminants. loop-mediated isothermal amplification (lamp) is a nucleic acid detection method in which the target dna can be efficiently amplified with high specificity and sensitivity under isothermal conditions. in this study, a lamp method was developed for the specific detection of a. ovis, using lamp primers designed on the basis of the major surface protein 4 gene. lamp was performed at 65°c for 30 min. its specificity was ... | 2011 | 21471346 |
| development of a loop-mediated isothermal amplification method for detection of theileria lestoquardi. | a loop-mediated isothermal amplification (lamp) assay was developed for the diagnosis of theileria lestoquardi infection. the primers were designed based on the clone-5 sequence of t. lestoquardi. the specificity and sensitivity of the assay were established. analysis of the specificity showed that the selected lamp primers amplified the target sequence from t. lestoquardi dna successfully, while no amplification was seen with dna from theileria annulata, theileria ovis, babesia ovis, anaplasma ... | 2011 | 21744022 |
| first molecular detection of theileria ovis in rhipicephalus sanguineus tick in iran. | to determine tick infestation of domestic ruminants and their infection to ovine theileriosis in northern iran. | 2012 | 22182639 |
| Babesia ovis as the main causative agent of sheep babesiosis in Iran. | Babesiosis is a haemoparasitic disease with high economical losses in livestock industry worldwide. The early diagnosis and successful therapy of babesiosis belong to the key steps of control and health management of livestock. Ethanol-fixed blood samples of 400 sheep were analyzed for Babesia infection. Reverse line blot (RLB) was established specifically for Theileria lestoquardi, Theileria (China 1), Theileria (China 2), Theileria ovis, Theileria separata, Babesia ovis, Babesia motasi, Babesi ... | 2011 | 21975684 |
| a study on ovine tick-borne hemoprotozoan parasites (theileria and babesia) in the east black sea region of turkey. | in this study, the frequency of theileria and babesia species was assessed via reverse line blotting and blood smear-based diagnostic methods in small ruminants. a total of 201 apparently healthy animals from 26 randomly selected herds located in 4 locations (artvin, giresun, gumushane, and tokat) of east black sea region of turkey were investigated for the blood protozoans. in a polymerase chain reaction (pcr), the hypervariable v4 region of the 18s ribosomal rna gene was amplified with a set o ... | 2012 | 22231266 |
| seroprevalence of bovine theileriosis in northern china. | bovine theileriosis is a common disease transmitted by ticks, and can cause loss of beef and dairy cattle worldwide. here, an indirect enzyme-linked immunosorbent assay (ielisa) based on theileria luwenshuni surface protein (tlsp) was developed and used to carry out a seroepidemiological survey of bovine theileriosis in northern china. | 2016 | 27863521 |
| blood parasites of livestock in certain regions in saudi arabia. | blood samples from camels, sheep, goats and cattle from six regions in saudi arabia were examined for blood parasites. asir region camels were disinfected while those of the eastern, jazan, northern frontiers, riyadh and tabouk regions were infected with trypanosoma evansi (5-40%), those of riyadh and the eastern regions were infected with dipetalonema evansi (1-6%) and those of the eastern, jazan and riyadh regions were infected with eperythrozoon species (8-20%). sheep and goats of all tested ... | 0 | 23961044 |
| assessment of exposure to piroplasms in sheep grazing in communal mountain pastures by using a multiplex dna bead-based suspension array. | piroplasms are tick-borne hemoprotozoans with a major impact on extensive management systems. detection of sub-clinical low-level carriers, which can act as source of infection for vector ticks, is key to protect livestock trade and facilitate preventive control programs. the purpose of this study was to develop a method for the detection of ovine piroplasms and to use it in a field study aimed at investigating piroplasms infection in semi-extensive production systems in the basque country (nort ... | 2013 | 24499621 |
| molecular detection and characterization of tick-borne pathogens in dogs and ticks from nigeria. | only limited information is currently available on the prevalence of vector borne and zoonotic pathogens in dogs and ticks in nigeria. the aim of this study was to use molecular techniques to detect and characterize vector borne pathogens in dogs and ticks from nigeria. | 2013 | 23505591 |
| canine and ovine tick-borne pathogens in camels, nigeria. | in april 2008, whole blood samples were collected from 36 dromedary camels in sokoto, north-western nigeria. following pcr and reverse line blotting, twenty-two samples (61%) resulted positive for ehrlichia/anaplasma spp. and three (8%) for theileria/babesia spp., with three (8%) cases of co-infections being found. both sequence and blast analyses identified ehrlichia/anaplasma spp. and theileria/babesia spp. positive cases as anaplasma platys and theileria ovis, respectively. this is the first ... | 2016 | 27692338 |
| prevalence of piroplasms in small ruminants in north-west tunisia and the first genetic characterisation of babesia ovis in africa. | in this study, the prevalence of piroplasms in sheep and goats was assessed with giemsa-stained blood smear examination, pcr and nested pcr-restriction fragment length polymorphism (rflp) to identify babesia and theileria species, respectively, in 338 small ruminants (172 sheep and 166 goats) from three sites in north-west tunisia during the 2011 summer season. the overall infection prevalence of piroplasms in giemsa-stained blood smears was 3.2% (11/338), with a parasitaemia ranging from 0.01 t ... | 2014 | 24849588 |
| molecular evidence of tick-borne hemoprotozoan-parasites (theileria ovis and babesia ovis) and bacteria in ticks and blood from small ruminants in northern algeria. | using qpcr, standard pcr and/or sequencing, we investigated the presence of tick-associated microorganisms in ticks and blood from sheep and goats from souk ahras, algeria. borrelia theileri, was detected in (7/120, 5.8%) blood from sheep and (13/120, 10.8%) goats. anaplasma ovis was screened in (38/73, 52%) rhipicephalus bursa and (5/22, 22.7%) r. turanicus and in (74/120, 61.7%), (65/120, 54.2%) blood of sheep and goats respectively. coxiella burnetii tested positive in r. bursa (4/73, 5.5%) a ... | 2017 | 28131376 |
| molecular detection and genetic characterization of babesia, theileria and anaplasma amongst apparently healthy sheep and goats in the central region of turkey. | babesia spp., theileria spp. and anaplasma spp. are significant tick-borne pathogens of livestock globally. in this study, we investigated the presence and distribution of babesia ovis, theileria ovis and anaplasma ovis in 343 small ruminants (249 sheep and 94 goats) from 13 towns in the central anatolia region of turkey using species-specific pcr assays. the pcr were conducted using the primers based on the b. ovis ssu rrna (bossurrna), t. ovis ssu rrna (tossurrna) and a. ovis major surface pro ... | 2017 | 27908771 |
| risks of suffering tick-borne diseases in sheep translocated to a tick infested area: a laboratory approach for the investigation of an outbreak. | this study was designed to investigate an outbreak of high mortality that occurred in naïve assaf sheep introduced into a latxa sheep flock in the basque country, a region where piroplasmosis is endemic. to identify the causes of this outbreak, a panel of different methods, including traditional pathological, biopathological and parasitological analyses combined with recently developed molecular methods, was used. these novel molecular methods included a multiplex real-time pcr assay to screen f ... | 2015 | 25257849 |
| coinfection of sheep with anaplasma, theileria and babesia species in the kurdistan region, iraq. | infections of small ruminants with anaplasma, theileria and babesia species are widely distributed in the old world and are of great economic impact. in iraq, data on disease occurrence in sheep caused by above-mentioned infectious agents are scarce. this study provides information on various haemoparasitic agents infecting sheep in the kurdistan region, iraq, using molecular diagnostic tools. altogether, 195 samples originating from three governorates in the kurdistan region, namely duhok, erbi ... | 2013 | 24589110 |
| tick-borne agents in domesticated and stray cats from the city of campo grande, state of mato grosso do sul, midwestern brazil. | anaplasmataceae agents, piroplasmids and hepatozoon spp. have emerged as important pathogens among domestic and wild felines. the present work aimed to detect the presence of species belonging to the anaplasmataceae family, piroplasmas and hepatozoon spp. dna in blood samples of domesticated and stray cats in the city of campo grande, state of mato grosso do sul, midwestern brazil. between january and april 2013, whole blood samples were collected from 151 cats (54 males, 95 females and two with ... | 2015 | 26187416 |
| molecular identification of theileria and babesia in ticks collected from sheep and goats in the black sea region of turkey. | a molecular survey was undertaken in the black sea region of turkey to determine the presence of theileria and babesia species of medical and veterinary importance. the ticks were removed from sheep and goats, pooled according to species and locations, and analyzed by pcr-based reverse line blot (rlb) and sequencing. a total of 2241 ixodid ticks belonging to 5 genus and 12 species were collected and divided into 310 pools. infection rates were calculated as the maximum likelihood estimation (mle ... | 2015 | 25260692 |
| molecular detection of theileria spp. and babesia spp. in sheep and ixodid ticks from the northeast of iran. | theilerioses and babesioses are important diseases in iranian sheep. the present study was undertaken to identify and classify/specify theileria spp. and babesia spp. in sheep and vector ticks. investigation was carried out from 2009 to 2011 in the khorasan razavi province, iran. in total, 302 sheep originating from 60 different flocks were clinically examined and their blood collected. in addition, from the same flocks, ixodid ticks were sampled. stained blood smears were microscopically examin ... | 2013 | 22924924 |
| molecular detection of theileria, babesia, and hepatozoon spp. in ixodid ticks from palestine. | ixodid ticks transmit various infectious agents that cause disease in humans and livestock worldwide. a cross-sectional survey on the presence of protozoan pathogens in ticks was carried out to assess the impact of tick-borne protozoa on domestic animals in palestine. ticks were collected from herds with sheep, goats and dogs in different geographic districts and their species were determined using morphological keys. the presence of piroplasms and hepatozoon spp. was determined by pcr amplifica ... | 2016 | 26969489 |
| development of loop-mediated isothermal amplification assay for specific and rapid detection of differential goat pox virus and sheep pox virus. | capripox viruses are economically important pathogens in goat and sheep producing areas of the world, with specific focus on goat pox virus (gtpv), sheep pox virus (sppv) and the lumpy skin disease virus (lsdv). clinically, sheep pox and goat pox have the same symptoms and cannot be distinguished serologically. this presents a real need for a rapid, inexpensive, and easy to operate and maintain genotyping tool to facilitate accurate disease diagnosis and surveillance for better management of cap ... | 2014 | 24438089 |
| molecular surveillance of theileria ovis, theileria lestoquardi and theileria annulata infection in sheep and ixodid ticks in iran. | a molecular study was undertaken to detect theileria ovis, theileria lestoquardi and theileria annulata in sheep and tick vectors. investigation was conducted from 2010 to 2011 in the south of khorasan razavi province, iran. a total of 150 blood samples were collected from 30 different sheep flocks. in addition, ixodid ticks were sampled from the same flocks. the stained blood smears were microscopically examined for the presence of piroplasms and a semi-nested polymerase chain reaction-restrict ... | 2013 | 24396911 |
| molecular detection of piroplasms in ixodid ticks infesting cattle and sheep in western oromia, ethiopia. | in ethiopia, ticks and tick-borne diseases are widely distributed and contribute to important economic losses. several studies investigated the prevalence and species composition of ticks infesting ruminants; however, data on tick-borne pathogens are still scarce. during the study period from october 2010 to april 2011, a total of 1,246 adult ticks and 264 nymphs were collected from 267 cattle and 45 sheep in bako district, western oromia, ethiopia. the study showed infestation of 228/267 (85.4 ... | 2014 | 23846769 |
| a molecular study of tick-borne haemoprotozoan parasites (theileria and babesia) in small ruminants in northern tunisia. | in this study, the frequency of theileria and babesia species in sheep and goats was assessed via reverse line blotting (rlb). a total of 263 apparently healthy sheep and goats, from 16 randomly selected flocks located in 9 localities situated in 3 bioclimatic zones in tunisia, were investigated for the blood protozoans. rlb hybridization with polymerase chain reaction detected only theileria ovis in sheep and goats, accounting for 22.4% (95% confidence interval [ci]: 17.6-27.1%) positive sample ... | 2013 | 24029713 |
| prevalence of tick-borne haemoparasites in small ruminants in turkey and diagnostic sensitivity of single-pcr and rlb. | tick-borne haemoparasitic diseases (tbhds), caused by theileria, babesia, anaplasma and ehrlichia, are common in regions of the world where the distributions of host, pathogen and vector overlap. many of these diseases threaten livestock production and some also represent a concern to human public health. the primary aim of this study was to determine the prevalence of the above-mentioned pathogens in a large number of blood samples (n = 1979) collected from sheep (n = 1727) and goats (n = 252) ... | 2017 | 28449722 |
| identification of novel theileria genotypes from grant's gazelle. | blood samples collected from grant's gazelles (nanger granti) in kenya were screened for hemoparasites using a combination of microscopic and molecular techniques. all 69 blood smears examined by microscopy were positive for hemoparasites. in addition, theileria/babesia dna was detected in all 65 samples screened by pcr for a ~450-base pair fragment of the v4 hypervariable region of the 18s rrna gene. sequencing and blast analysis of a subset of pcr amplicons revealed widespread co-infection (25 ... | 2015 | 25973394 |
| molecular detection and characterization of theileria infecting wildebeest (connochaetes taurinus) in the maasai mara national reserve, kenya. | theileria is a genus of tick-borne protozoan that is globally widespread and infects nearly all ungulates in which they cause either latent infection or lethal disease. wild animals are considered reservoir hosts of many species of theileria and their diversity in wildlife species is increasingly becoming of interest. the molecular characterization and identification of theileria infecting wildlife has been studied in a few species including buffalo, which are considered reservoir host for theil ... | 2015 | 26295263 |
| molecular identification of different theileria and babesia species infecting sheep in sudan. | the epidemiological aspects of sheep piroplasmosis in sudan are poorly studied, and further investigations using sensitive and precise techniques are required. in this study, the reverse line blot (rlb) hybridization assay was used to detect and simultaneously differentiate between theileria and babesia species. dna was extracted from blood collected on filter paper (n=219) from apparently healthy sheep from six different geographical localities in sudan. results indicated that theileria ovis (8 ... | 2016 | 27262957 |
| molecular identification of theileria and babesia in sheep and goats in the black sea region in turkey. | this study was carried out to investigate presence and distribution of theileria and babesia species via microscopic examination and reverse line blotting (rlb) techniques in sheep and goats in the black sea region of turkey. for this purpose, 1,128 blood samples (869 sheep and 259 goats) were collected by active surveillance from sheep and goats in different provinces of various cities in the region in the years 2010 and 2011. smears were prepared from the blood samples, stained with giemsa, an ... | 2013 | 23689604 |
| prevalence and molecular diagnosis of babesia ovis and theileria ovis in lohi sheep at livestock experiment station (les), bahadurnagar, okara, pakistan. | babesia ovis and theileria ovis are among the important and main etiological agents causing ovine babesiosis and ovine theileriosis, causing severe economic losses among sheep and goats. the aim of the present study was to determine the prevalence and molecular diagnosis of b. ovis and t. ovis in lohi sheep at livestock experiment station bahadurnagar, okara, pakistan. | 2013 | 25516738 |
| prevalence of theileria and babesia species in tunisian sheep. | in this study, the prevalence of theileria and babesia species in sheep was assessed with giemsastained blood smear examination and polymerase chain reaction to identify the different piroplasms in 270 sheep from three tunisian bioclimatic zones (north, centre, and south). the overall infection prevalence by babesia spp. and theileria spp. in giemsa-stained blood smears was 2.9% (8/270) and 4.8% (13/270) respectively. the molecular results showed that sheep were more often infected by theileria ... | 2016 | 27247070 |
| molecular and parasitological survey of ovine piroplasmosis, including the first report of theileria annulata (apicomplexa: theileridae) in sheep and goats from turkey. | blood and tick samples were collected from 333 apparently healthy sheep and 257 goats as well as 10 sheep exhibiting clinical signs of babesiosis in adana, gaziantep, and adiyaman provinces in southern turkey. fully engorged female ticks were selected and maintained in an incubator until they oviposited. the tick carcasses and their egg masses were examined. piroplasms compatible with babesia spp. and theileria spp. were observed in both symptomatic and asymptomatic small ruminants. genomic dna ... | 2016 | 27559124 |
| ticks threatening lineage of anatolian wild sheep (ovis gmelinii anatolica) and determination of their tick-borne pathogens. | we aimed to determine the ticks of the anatolian wild sheep and to define their tick-borne pathogens while molecularly studying their relationships with those of the domestic sheep. furthermore, another aim of this study is to investigate tick paralysis resulting in the death of the anatolian wild sheep. ticks and blood samples were collected from the wild sheep whilst tick samples were also collected from hares, guinea fowls, chickens, and a turkey living in the anatolian wild sheep breeding ar ... | 2016 | 27692336 |
| prevalence of <i>theileria</i> and <i>babesia</i> species in tunisian sheep. | in this study, the prevalence of theileria and babesia species in sheep was assessed with giemsastained blood smear examination and polymerase chain reaction to identify the different piroplasms in 270 sheep from three tunisian bioclimatic zones (north, centre, and south). the overall infection prevalence by babesia spp. and theileria spp. in giemsa-stained blood smears was 2.9% (8/270) and 4.8% (13/270) respectively. the molecular results showed that sheep were more often infected by theileria ... | 2016 | 27796112 |
| molecular and parasitological survey of ovine piroplasmosis, including the first report of theileria annulata (apicomplexa: theileridae) in sheep and goats from turkey. | blood and tick samples were collected from 333 apparently healthy sheep and 257 goats as well as 10 sheep exhibiting clinical signs of babesiosis in adana, gaziantep, and adiyaman provinces in southern turkey. fully engorged female ticks were selected and maintained in an incubator until they oviposited. the tick carcasses and their egg masses were examined. piroplasms compatible with babesia spp. and theileria spp. were observed in both symptomatic and asymptomatic small ruminants. genomic dna ... | 2017 | 28082649 |
| multiplex pcr for diagnosis of theileria uilenbergi, theileria luwenshuni, and theileria ovis in small ruminants. | infections with theileria sp. may cause significant economic losses to the sheep industry. species identification based on microscopic examination is difficult, and more suitable methods are required for the rapid detection and identification of theileria sp, in clinical specimens. in this study, a multiplex polymerase chain reaction (mpcr) assay was developed to simultaneously identify three individual theileria species in small ruminants. three pairs of specific, sensitive primers were designe ... | 2014 | 24241125 |
| an indirect elisa for detection of theileria spp. antibodies using a recombinant protein (rtlsp) from theileria luwenshuni. | theileria is a tick-borne, intracellular protozoan parasite of worldwide economic and veterinary importance in small ruminants. here, an enzyme-linked immunosorbent assay (elisa) was developed based on theileria luwenshuni recombinant surface protein (rtlsp) and was used in the standardization and validation of an elisa for the detection of circulating antibodies against ovine and caprine theileriosis. a total of 233 sera samples were used for the calculation of the cut-off value which served as ... | 2016 | 27048941 |
| molecular detection of theileria spp. in sheep and vector ticks in the north khorasan province, iran. | a survey was carried in north khorasan province, iran in 2010-2011, designed to identify theileria spp. infections of both sheep and ticks. the tick species were also examined. ninety sheep from different flocks were clinically examined, and blood samples and ixodid ticks were collected. light microscopy of blood smears revealed theileria spp. infection in 37 (41.1 %), while 74 (82.2 %) of blood samples were positive using semi-nested pcr. theileria ovis, theileria lestoquardi, and mixed infecti ... | 2013 | 22791188 |
| identification and sequence characterization of novel theileria genotypes from the waterbuck (kobus defassa) in a theileria parva-endemic area in kenya. | waterbuck (kobus defassa), an ungulate species endemic to the eastern african savannah, is suspected of being a wildlife reservoir for tick-transmitted parasites infective to livestock. waterbuck is infested by large numbers of rhipicephalus appendiculatus, the tick vector for theileria parva, and previous data suggests that the species may be a source of t. parva transmission to cattle. in the present study, a total of 86 cattle and 26 waterbuck blood samples were obtained from marula, a site i ... | 2014 | 24690249 |
| identification and characterization of theileria ovis surface protein (tosp) resembled tasp in theileria annulata. | ovine theileriosis is an important hemoprotozoal disease of sheep and goats in tropical and subtropical regions which caused high economic loses in the livestock industry. theileria annulata surface protein (tasp) was used previously as a tool for serological analysis in livestock. since the amino acid sequences of tasp is, at least, in part very conserved in t. annulata, theileria lestoquardi and theileria china i and ii, it is very important to determine the amino acid sequence of this protein ... | 2016 | 26833323 |
| genetic variability, phylogenetic evaluation and first global report of theileria luwenshuni, t. buffeli, and t. ovis in sheepdogs in iran. | theileriosis, caused by tick-borne hematozoan organisms, is a protozoan disease of domestic and wild mammals. the most phylo-molecular explorations of genus theileria have been globally focused on the livestock and here are few investigations about canine theileriosis. twenty three and 52 blood samples were collected from sheepdogs and their owners, respectively, at different geographical foci of mazandaran province (east, central, and west regions), northern iran. to primary identify, the taken ... | 2016 | 26997339 |