Publications

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functional immunomics of the squash bug, anasa tristis (de geer) (heteroptera: coreidae).the squash bug, anasa tristis (de geer), is a major piercing/sucking pest of cucurbits, causing extensive damage to plants and fruits, and transmitting phytopathogens. no genomic resources to facilitate field and laboratory studies of this pest were available; therefore the first de novo exome for this destructive pest was assembled. rna was extracted from insects challenged with bacterial and fungal immunoelicitors, insects fed on different cucurbit species, and insects from all life stages fro ...201326462532
metabolism during embryonic and metamorphic development of insects.1. for species of insects that deposit their eggs on foliage, as leptinotarsa decemlineata, crioceris asparagi, and anasa tristis, the rates of metabolism as measured by the co(2) output or the oxygen intake indicate, during embryonic development, a short formative period, followed by a very active extended growth. those species that deposit their eggs in the soil, as cotinis nitida and popillia japonica, show that the formative period is greatly extended and the growth period similarly lengthen ...192519872155
watermelon seedling growth and mortality as affected by anasa tristis (heteroptera: coreidae).adult squash bugs, anasa tristis (de geer), were confined on seedling watermelon plants at densities of zero, one, two, and four per plant. squash bugs were allowed to feed on the plants until plants died or reached 30 cm in height. number of leaves and length of plant vine were recorded at 2- or 3-d intervals. seedling foliage, stems, and roots were harvested and dried after plants reached 30 cm in height. growth of seedlings was regressed on number of squash bugs and results indicated that an ...200212076005
watermelon growth, fruit yield and plant survival as affected by squash bug (hemiptera: coreidae) feeding.adult squash bugs, anasa tristis (de geer) (heteroptera: coreidae), were confined on watermelon plants at differing phenological stages and at densities of zero to four per plant in one trial and zero to 32 per plant in three additional trials. squash bugs were allowed to feed on the plants until plants died or fruit matured. plant foliage, roots, and fruit were harvested and weighed to determine effects on growth and productivity. growth and fruit production was regressed on number of squash bu ...200312650346
a survey of the species of squash bug (hemiptera: coreidae) egg parasitoids in virginia and their distribution.squash bug, anasa tristis degeer (hemiptera: coreidae), is a major pest of squash and pumpkins in the united states. in order to better understand the importance of natural egg parasitism of this species in virginia, we conducted a 2-yr statewide survey. in total, 1,127 squash bug egg masses (~20,000 total eggs) were sampled from squash and pumpkins from 43 counties in virginia from 2014 to 2015. egg masses were brought back to the lab to record levels of squash bug nymphal emergence or adult pa ...201729126157
the case of anasa tristis. 190717755005
chemical attraction of kleptoparasitic flies to heteropteran insects caught by orb-weaving spiders.insects of the heteropteran families pentatomidae (stink bugs) and coreidae (squash bugs), when being eaten by the orb-weaving spider nephila clavipes, attract flies of the family milichiidae. the flies aggregate on the bugs and, as kleptoparasites, share in the spider's meal. stink bugs and squash bugs typically eject defensive sprays when attacked; they do so when caught by nephila, but the spray only minimally affects the spider. evidence is presented indicating that it is the spray of the bu ...19911896468
comparison of management strategies for squash bugs (hemiptera: coreidae) in watermelon.two watermelon pest management practices, a squash trap crop and a standard recommendation using soil-applied carbofuran, were compared using large-scale field plots to assess trap crop suitability as a replacement for the standard in 2000, 2001, and 2002. in both systems, foliar insecticide applications were used to control squash bugs when populations exceeded threshold levels. during 2001 and 2002, a treatment of untreated watermelon was used. early season adult insects, from seedling to frui ...200415666757
predator-prey trophic relationships in response to organic management practices.a broad range of environmental conditions likely regulate predator-prey population dynamics and impact the structure of these communities. central to understanding the interplay between predator and prey populations and their importance is characterizing the corresponding trophic interactions. here, we use a well-documented molecular approach to examine the structure of the community of natural enemies preying upon the squash bug, anasa tristis, a herbivorous cucurbit pest that severely hinders ...201424673741
impact of the egg parasitoid, gryon pennsylvanicum (hymenoptera: scelionidae), on sentinel and wild egg masses of the squash bug (hemiptera: coreidae) in maryland.seasonal changes in egg parasitism and predation rates on sentinel (laboratory-reared) and wild (naturally occurring) egg masses of the squash bug, anasa tristis (de geer), were evaluated in squash fields in maryland from june through september in 2013 and 2014. rates of egg parasitism were significantly higher on wild egg masses than on sentinel egg masses. squash bug nymphal emergence was significantly higher on sentinel egg masses than on wild egg masses. between the first week of july and th ...201626759367
cell lines derived from the squash bug, anasa tristis (coreidae: hemiptera).the squash bug, anasa tristis, is a pest of cucurbits that exerts direct damage on crops and is a vector of plant pathogens. we established cell lines from this insect to serve as tools for basic biology, including virology and immunology, as well as applied studies, such as insecticide development programs. we initiated 15 cell cultures, using nine media or combinations of media. the media yielding the best results were a modification of kimura's medium and a combination of two commercially ava ...201728205141
assessment of habitat modification and varied planting dates to enhance potential natural enemies of anasa tristis (hemiptera: coreidae) in squash.the squash bug, anasa tristis (degeer) (hemiptera: coreidae), is an indigenous pest of squash and other cucurbits. pesticides can control squash bug populations although many small-scale growers in the southeast seek alternative methods of management. cultural control methods, including varying the planting date and farmscaping, are not well understood under southeastern conditions. the goal of farmscaping is to increase natural ecosystem functions to aid in the control of pest populations. in t ...201728334210
prostaglandin actions in established insect cell lines.prostaglandins (pgs) are oxygenated metabolites of arachidonic acid (aa) and two other c20 polyunsaturated fatty acids that serve as biochemical signals mediating physiological functions. we reported that pgs influence protein expression in insect cell lines, which prompted the question: do pgs influence cell proliferation or viability in insect cell lines? here, we report on the outcomes of experiments designed to address the question in cell lines from three insect orders: hemiptera (squash bu ...201728455813
toads killed by squash-bugs. 190117773545
rate of circulation of the body fluid in adult tenebrio molitor linnaeus, anasa tristis (de goer), and murgantia histrionica (hahn). 195114845697
overwintering squash bugs harbor and transmit the causal agent of cucurbit yellow vine disease.since 1988, cucurbit crops, particularly watermelon, cantaloupe, and squash, grown in oklahoma and texas have experienced devastating losses from cucurbit yellow vine disease (cyvd), caused by the phloem-limited bacterium serratia marcescens bizio. squash bug, anasa tristis (de geer), is a putative vector of the pathogen. in 2000-2001, overwintering populations of squash bug collected from deleon, tx, were tested for their ability to harbor and transmit the bacterium. individual squash bugs (n = ...200414998129
effects of cucumber mosaic virus infection on vector and non-vector herbivores of squash.plant chemicals mediating interactions with insect herbivores seem a likely target for manipulation by insectvectored plant pathogens. yet, little is currently known about the chemical ecology of insect-vectored diseases or their effects on the ecology of vector and nonvector insects. we recently reported that a widespread plant pathogen, cucumber mosaic virus (cmv), greatly reduces the quality of host-plants (squash) for aphid vectors, but that aphids are nevertheless attracted to the odors of ...201021331245
companion planting with white yarrow or with feverfew for squash bug, anasa tristis (hemiptera: coreidae), management on summer squash.the squash bug, anasa tristis (de geer), is a major insect pest of cucurbits. control of squash bugs with insecticidal chemicals is difficult to achieve. we investigated the potential of companion planting with white yarrow (achillea millefolium l.) or feverfew [tanacetum parthenium (l.) sch. bip.] for squash bug management in field plantings of summer squash (cucurbita pepo l.).201627604891
seasonal phenology and natural enemies of the squash bug (hemiptera: coreidae) in kentucky.the squash bug, anasa tristis (de geer), is a major indigenous pest of cucurbita species across the united states and a vector of cucurbit yellow vine disease. the seasonal phenology of the squash bug in central kentucky and its natural enemies were studied using summer squash planted sequentially throughout the 2005 and 2006 growing seasons. the squash bug was first detected on 5 june 2005 and 3 june 2006. in both years, peak numbers of all squash bug stages occurred in july and august. our fie ...200818559172
spatial pattern and sequential sampling of squash bug (heteroptera: coreidae) adults in watermelon.spatial distribution patterns of adult squash bugs were determined in watermelon, citrullus lanatus (thunberg) matsumura and nakai, during 2001 and 2002. results of analysis using taylor's power law regression model indicated that squash bugs were aggregated in watermelon. taylor's power law provided a good fit with r2 = 0.94. a fixed precision sequential sampling plan was developed for estimating adult squash bug density at fixed precision levels in watermelon. the plan was tested using a resam ...200616686160
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