| detection of xylella fastidiosa in potential insect vectors by immunomagnetic separation and nested polymerase chain reaction. | a sensitive and specific assay for detecting xylella fastidiosa in potential insect vectors was developed. this assay involves immunomagnetic separation of the bacteria from the insect, followed by a two-step, nested polymerase chain reaction (pcr) amplification using previously developed oligonucleotide primers specific to x. fastidiosa. a total of 347 leafhoppers representing 16 species were captured and sampled from american elm (ulmus americana l.) trees growing in a nursery where bacterial ... | 1997 | 9281861 |
| seasonal abundance and natural inoculativity of insect vectors of xylella fastidiosa in oklahoma tree nurseries and vineyards. | xylella fastidiosa is the causative agent of diseases of perennial plants including peach, plum, elm, oak, pecan, and grape. this bacterial pathogen is transmitted by xylem-feeding insects. in recent years, pierce's disease of grape has been detected in 10 counties in central and northeastern oklahoma, prompting further investigation of the disease epidemiology in this state. we surveyed vineyards and tree nurseries in oklahoma for potential insect vectors to determine species composition, infec ... | 2015 | 26331482 |